Extracellular vesicles: Taking metabolism on the road

Posted on Aug 18th, 2017 - By Lucas B Sullivan

Nature Chemical Biology 13, 924 (2017). doi:10.1038/nchembio.2455 Author: Lucas B Sullivan Extracellular vesicles (EVs) are a class of secreted membrane particles capable of transferring biological molecules between cells. Metabolomics measurements indicate that isolated EVs also have autonomous metabolic enzyme activities, including the unexpected identification of endogenous human asparaginase…

Continue Reading

Protein design: I like to fold it, fold it

Posted on Aug 18th, 2017 - By Caitlin Deane

Nature Chemical Biology 13, 923 (2017). doi:10.1038/nchembio.2467 Author: Caitlin…

Continue Reading

Neurobiology: Lighting up neurons

Posted on Aug 18th, 2017 - By Grant Miura

Nature Chemical Biology 13, 923 (2017). doi:10.1038/nchembio.2469 Author: Grant…

Continue Reading

Bacterial immunity: A virtuous CRISPR cycle

Posted on Aug 18th, 2017 - By Terry L. Sheppard

Nature Chemical Biology 13, 923 (2017). doi:10.1038/nchembio.2468 Author: Terry L.…

Continue Reading

Drug development: Locking down metabolism

Posted on Aug 18th, 2017 - By William R Bishai

Nature Chemical Biology 13, 925 (2017). doi:10.1038/nchembio.2452 Author: William R Bishai An allosteric inhibitor of Mycobacterium tuberculosis tryptophan synthase—an enzyme that is nonessential for in vitro growth—has potent antimicrobial activity, revealing a potentially expanded target list for antimicrobials and greater chemical space for new…

Continue Reading

A kinetic view of GPCR allostery and biased agonism

Posted on Aug 18th, 2017 - By J Robert Lane

Nature Chemical Biology 13, 929 (2017). doi:10.1038/nchembio.2431 Authors: J Robert Lane, Lauren T May, Robert G Parton, Patrick M Sexton & Arthur…

Continue Reading

Ubiquitin-proteasome system: Rescuing EBV latency

Posted on Aug 18th, 2017 - By Mirella Bucci

Nature Chemical Biology 13, 923 (2017). doi:10.1038/nchembio.2466 Author: Mirella…

Continue Reading

Protein engineering: Redirecting membrane machinery

Posted on Aug 18th, 2017 - By Kalistyn Burley

Nature Chemical Biology 13, 927 (2017). doi:10.1038/nchembio.2451 Authors: Kalistyn Burley & Celia W Goulding The ability to solubilize membrane proteins while retaining their native function is a persistent challenge. Re-engineering of the membrane protein DsbB into a soluble cytoplasmic version maintained its activity and enabled recompartmentalization of the periplasmic DsbAB disulfide bond–forming…

Continue Reading

Copper import in Escherichia coli by the yersiniabactin metallophore system

Posted on Jul 24th, 2017 - By Eun-Ik Koh

Nature Chemical Biology 13, 1016 (2017). doi:10.1038/nchembio.2441 Authors: Eun-Ik Koh, Anne E Robinson, Nilantha Bandara, Buck E Rogers & Jeffrey P…

Continue Reading

FRET monitoring of a nonribosomal peptide synthetase

Posted on Jul 24th, 2017 - By Jonas Alfermann

Nature Chemical Biology 13, 1009 (2017). doi:10.1038/nchembio.2435 Authors: Jonas Alfermann, Xun Sun, Florian Mayerthaler, Thomas E Morrell, Eva Dehling, Gerrit Volkmann, Tamiki Komatsuzaki, Haw Yang & Henning D…

Continue Reading

Toxicity and repair of DNA adducts produced by the natural product yatakemycin

Posted on Jul 24th, 2017 - By Elwood A Mullins

Nature Chemical Biology 13, 1002 (2017). doi:10.1038/nchembio.2439 Authors: Elwood A Mullins, Rongxin Shi & Brandt F…

Continue Reading

Peptidomimetic inhibitors of APC–Asef interaction block colorectal cancer migration

Posted on Jul 24th, 2017 - By Haiming Jiang

Nature Chemical Biology 13, 994 (2017). doi:10.1038/nchembio.2442 Authors: Haiming Jiang, Rong Deng, Xiuyan Yang, Jialin Shang, Shaoyong Lu, Yanlong Zhao, Kun Song, Xinyi Liu, Qiufen Zhang, Yu Chen, Y Eugene Chinn, Geng Wu, Jian Li, Guoqiang Chen, Jianxiu Yu & Jian…

Continue Reading

Functional annotation of chemical libraries across diverse biological processes

Posted on Jul 24th, 2017 - By Jeff S Piotrowski

Nature Chemical Biology 13, 982 (2017). doi:10.1038/nchembio.2436 Authors: Jeff S Piotrowski, Sheena C Li, Raamesh Deshpande, Scott W Simpkins, Justin Nelson, Yoko Yashiroda, Jacqueline M Barber, Hamid Safizadeh, Erin Wilson, Hiroki Okada, Abraham A Gebre, Karen Kubo, Nikko P Torres, Marissa A LeBlanc, Kerry Andrusiak, Reika Okamoto, Mami Yoshimura, Eva DeRango-Adem, Jolanda van Leeuwen, Katsuhiko Shirahige, Anastasia Baryshnikova, Grant W Brown, Hiroyuki Hirano, Michael…

Continue Reading

Redox regulation: Scaffolding H2O2 signaling

Posted on Jul 18th, 2017 - By Hadley D Sikes

Nature Chemical Biology 13, 818 (2017). doi:10.1038/nchembio.2432 Author: Hadley D Sikes Peroxidases, rather than simply reducing H2O2 to water, also convey oxidation signals to proteins such as transcription factors. A new study reveals how a scaffold protein enables formation of a mixed disulfide between the peroxidase and a transcription…

Continue Reading

Enzymology: Tracking off-targets

Posted on Jul 18th, 2017 - By Alison Farrell

Nature Chemical Biology 13, 817 (2017). doi:10.1038/nchembio.2445 Author: Alison…

Continue Reading

Erratum: The EED protein–protein interaction inhibitor A-395 inactivates the PRC2 complex

Posted on Jul 18th, 2017 - By Yupeng He

Nature Chemical Biology 13, 922 (2017). doi:10.1038/nchembio0817-922b Author: Yupeng He, Sujatha Selvaraju, Michael L Curtin, Clarissa G Jakob, Haizhong Zhu, Kenneth M Comess, Bailin Shaw, Juliana The, Evelyne Lima-Fernandes, Magdalena M Szewczyk, Dong Cheng, Kelly L Klinge, Huan-Qiu Li, Marina Pliushchev, Mikkel A Algire, David Maag, Jun Guo, Justin Dietrich, Sanjay C Panchal, Andrew M Petros, Ramzi F Sweis, Maricel Torrent, Lance J Bigelow, Guillermo Senisterra, Fengling Li,…

Continue Reading

Antibacterials: PUMmeling RNAP

Posted on Jul 18th, 2017 - By Terry L. Sheppard

Nature Chemical Biology 13, 817 (2017). doi:10.1038/nchembio.2446 Author: Terry L.…

Continue Reading

Erratum: Structural and conformational determinants of macrocycle cell permeability

Posted on Jul 18th, 2017 - By Björn Over

Nature Chemical Biology 13, 922 (2017). doi:10.1038/nchembio0817-922a Author: Björn Over, Pär Matsson, Christian Tyrchan, Per Artursson, Bradley C Doak, Michael A Foley, Constanze Hilgendorf, Stephen E Johnston, Maurice D Lee, Richard J Lewis, Patrick McCarren, Giovanni Muncipinto, Ulf Norinder, Matthew W D Perry, Jeremy R Duvall & Jan…

Continue Reading

Biocatalysis: Achieving aminase activity

Posted on Jul 18th, 2017 - By Caitlin Deane

Nature Chemical Biology 13, 817 (2017). doi:10.1038/nchembio.2447 Author: Caitlin…

Continue Reading

Harnessing yeast organelles for metabolic engineering

Posted on Jul 18th, 2017 - By Sarah K Hammer

Nature Chemical Biology 13, 823 (2017). doi:10.1038/nchembio.2429 Authors: Sarah K Hammer & José L…

Continue Reading

Structural biology: Full monty of family B GPCRs

Posted on Jul 18th, 2017 - By Thue W Schwartz

Nature Chemical Biology 13, 819 (2017). doi:10.1038/nchembio.2438 Authors: Thue W Schwartz & Thomas M Frimurer New structures of the glucagon-like peptide-1 (GLP-1) and glucagon receptors in complex with peptide and nonpeptide ligands provide a comprehensive, detailed picture of the molecular mechanisms of action of family B GPCRs. This opens the door for true structure-based drug discovery aimed at both novel orthosteric and allosteric subsites of the…

Continue Reading

Hints of hidden chemistry

Nature Chemical Biology 13, 815 (2017). doi:10.1038/nchembio.2449 Biosynthetic pathways harbor diverse enzyme functions, and identifying those that catalyze unusual or synthetically challenging transformations offers new routes for biocatalytic…

Continue Reading

Biosynthesis: Methylating mushrooms

Posted on Jul 18th, 2017 - By Albert A Bowers

Nature Chemical Biology 13, 821 (2017). doi:10.1038/nchembio.2437 Author: Albert A Bowers The genome of the poisonous mushroom Omphalotus olearius provides a potent new biocatalytic strategy for installing backbone N-methyl amides on ribosomally synthesized peptides. This discovery could yield new biotechnologies for drug development from peptide…

Continue Reading

Neurodegenerative disease: Not stuck on repeat

Posted on Jul 18th, 2017 - By Mirella Bucci

Nature Chemical Biology 13, 817 (2017). doi:10.1038/nchembio.2444 Author: Mirella…

Continue Reading

Structures of carboxylic acid reductase reveal domain dynamics underlying catalysis

Posted on Jul 17th, 2017 - By Deepankar Gahloth

Nature Chemical Biology 13, 975 (2017). doi:10.1038/nchembio.2434 Authors: Deepankar Gahloth, Mark S Dunstan, Daniela Quaglia, Evaldas Klumbys, Michael P Lockhart-Cairns, Andrew M Hill, Sasha R Derrington, Nigel S Scrutton, Nicholas J Turner & David…

Continue Reading

An excited state underlies gene regulation of a transcriptional riboswitch

Posted on Jul 17th, 2017 - By Bo Zhao

Nature Chemical Biology 13, 968 (2017). doi:10.1038/nchembio.2427 Authors: Bo Zhao, Sharon L Guffy, Benfeard Williams & Qi…

Continue Reading

Allosteric sensitization of proapoptotic BAX

Posted on Jul 10th, 2017 - By Jonathan R Pritz

Nature Chemical Biology 13, 961 (2017). doi:10.1038/nchembio.2433 Authors: Jonathan R Pritz, Franziska Wachter, Susan Lee, James Luccarelli, Thomas E Wales, Daniel T Cohen, Paul Coote, Gregory J Heffron, John R Engen, Walter Massefski & Loren D…

Continue Reading

A new strategy for aromatic ring alkylation in cylindrocyclophane biosynthesis

Posted on Jun 26th, 2017 - By Hitomi Nakamura

Nature Chemical Biology 13, 916 (2017). doi:10.1038/nchembio.2421 Authors: Hitomi Nakamura, Erica E Schultz & Emily P…

Continue Reading

Targeting the N terminus for site-selective protein modification

Posted on Jun 20th, 2017 - By Christian B Rosen

Nature Chemical Biology 13, 697 (2017). doi:10.1038/nchembio.2416 Authors: Christian B Rosen & Matthew B…

Continue Reading

Metal homeostasis: Pumping iron

Posted on Jun 20th, 2017 - By Grant Miura

Nature Chemical Biology 13, 693 (2017). doi:10.1038/nchembio.2423 Author: Grant…

Continue Reading

Antimicrobials: Targeting fungal chromatin

Posted on Jun 20th, 2017 - By Stéphane Larochelle

Nature Chemical Biology 13, 693 (2017). doi:10.1038/nchembio.2424 Author: Stéphane…

Continue Reading

Circadian regulation: Switching periods

Posted on Jun 20th, 2017 - By Grant Miura

Nature Chemical Biology 13, 693 (2017). doi:10.1038/nchembio.2426 Author: Grant…

Continue Reading

Synthetic biology: Playing favorites

Posted on Jun 20th, 2017 - By Ross Cloney

Nature Chemical Biology 13, 693 (2017). doi:10.1038/nchembio.2425 Author: Ross…

Continue Reading

Peptidoglycan: Another brick in the wall

Posted on Jun 20th, 2017 - By Anthony J Clarke

Nature Chemical Biology 13, 695 (2017). doi:10.1038/nchembio.2419 Author: Anthony J Clarke Lipid II embodies the bricks used to build the essential bacterial cell wall component peptidoglycan. A facile new procedure for preparation of species-specific Lipid II in high yields can now be used to unlock the door to antibiotic…

Continue Reading

A scaffold protein that chaperones a cysteine-sulfenic acid in H2O2 signaling

Posted on Jun 19th, 2017 - By Antoine Bersweiler

Nature Chemical Biology 13, 909 (2017). doi:10.1038/nchembio.2412 Authors: Antoine Bersweiler, Benoît D'Autréaux, Hortense Mazon, Alexandre Kriznik, Gemma Belli, Agnès Delaunay-Moisan, Michel B Toledano & Sophie…

Continue Reading

Nucleation and growth of a bacterial functional amyloid at single-fiber resolution

Posted on Jun 19th, 2017 - By Mike Sleutel

Nature Chemical Biology 13, 902 (2017). doi:10.1038/nchembio.2413 Authors: Mike Sleutel, Imke Van den Broeck, Nani Van Gerven, Cécile Feuillie, Wim Jonckheere, Claire Valotteau, Yves F Dufrêne & Han…

Continue Reading

Cpf1 proteins excise CRISPR RNAs from mRNA transcripts in mammalian cells

Posted on Jun 19th, 2017 - By Guocai Zhong

Nature Chemical Biology 13, 839 (2017). doi:10.1038/nchembio.2410 Authors: Guocai Zhong, Haimin Wang, Yujun Li, Mai H Tran & Michael Farzan Cpf1 is a CRISPR effector protein that has greater specificity than Streptococcus pyogenes Cas9 (SpCas9) in genome-editing applications. Here we show that Lachnospiraceae bacterium (Lb) and Acidaminococus sp. (As) Cpf1 orthologs have RNase activities that can excise multiple CRISPR RNAs (crRNAs) from a single RNA polymerase…

Continue Reading

A heme-dependent enzyme forms the nitrogen–nitrogen bond in piperazate

Posted on Jun 19th, 2017 - By Yi-Ling Du

Nature Chemical Biology 13, 836 (2017). doi:10.1038/nchembio.2411 Authors: Yi-Ling Du, Hai-Yan He, Melanie A Higgins & Katherine S Ryan Molecules containing a nitrogen–nitrogen (N–N) linkage have a variety of structures and biological activities; however, no enzyme has yet been demonstrated to catalyze N–N bond formation in an organic molecule. Here we report that the heme-dependent enzyme KtzT from Kutzneria sp. 744 catalyzes N–N bond formation in the…

Continue Reading

Oxidation of phosphorothioate DNA modifications leads to lethal genomic instability

Posted on Jun 12th, 2017 - By Stefanie Kellner

Nature Chemical Biology 13, 888 (2017). doi:10.1038/nchembio.2407 Authors: Stefanie Kellner, Michael S DeMott, Ching Pin Cheng, Brandon S Russell, Bo Cao, Delin You & Peter C…

Continue Reading

Genetically encoding phosphotyrosine and its nonhydrolyzable analog in bacteria

Posted on Jun 12th, 2017 - By Xiaozhou Luo

Nature Chemical Biology 13, 845 (2017). doi:10.1038/nchembio.2405 Authors: Xiaozhou Luo, Guangsen Fu, Rongsheng E Wang, Xueyong Zhu, Claudio Zambaldo, Renhe Liu, Tao Liu, Xiaoxuan Lyu, Jintang Du, Weimin Xuan, Anzhi Yao, Sean A Reed, Mingchao Kang, Yuhan Zhang, Hui Guo, Chunhui Huang, Peng-Yu Yang, Ian A Wilson, Peter G Schultz & Feng…

Continue Reading

A mutant O-GlcNAcase enriches Drosophila developmental regulators

Posted on Jun 12th, 2017 - By Nithya Selvan

Nature Chemical Biology 13, 882 (2017). doi:10.1038/nchembio.2404 Authors: Nithya Selvan, Ritchie Williamson, Daniel Mariappa, David G Campbell, Robert Gourlay, Andrew T Ferenbach, Tonia Aristotelous, Iva Hopkins-Navratilova, Matthias Trost & Daan M F van…

Continue Reading

A front-face ‘SNi synthase’ engineered from a retaining ‘double-SN2’ hydrolase

Posted on Jun 12th, 2017 - By Javier Iglesias-Fernández

Nature Chemical Biology 13, 874 (2017). doi:10.1038/nchembio.2394 Authors: Javier Iglesias-Fernández, Susan M Hancock, Seung Seo Lee, Maola Khan, Jo Kirkpatrick, Neil J Oldham, Katherine McAuley, Anthony Fordham-Skelton, Carme Rovira & Benjamin G…

Continue Reading

A scalable platform to identify fungal secondary metabolites and their gene clusters

Posted on Jun 12th, 2017 - By Kenneth D Clevenger

Nature Chemical Biology 13, 895 (2017). doi:10.1038/nchembio.2408 Authors: Kenneth D Clevenger, Jin Woo Bok, Rosa Ye, Galen P Miley, Maria H Verdan, Thomas Velk, Cynthia Chen, KaHoua Yang, Matthew T Robey, Peng Gao, Matthew Lamprecht, Paul M Thomas, Md Nurul Islam, Jonathan M Palmer, Chengcang C Wu, Nancy P Keller & Neil L…

Continue Reading

Site-specific incorporation of phosphotyrosine using an expanded genetic code

Posted on Jun 12th, 2017 - By Christian Hoppmann

Nature Chemical Biology 13, 842 (2017). doi:10.1038/nchembio.2406 Authors: Christian Hoppmann, Allison Wong, Bing Yang, Shuwei Li, Tony Hunter, Kevan M Shokat & Lei Wang Access to phosphoproteins with stoichiometric and site-specific phosphorylation status is key to understanding the role of protein phosphorylation. Here we report an efficient method to generate pure, active phosphotyrosine-containing proteins by genetically encoding a stable phosphotyrosine analog that is…

Continue Reading

Blocking an N-terminal acetylation–dependent protein interaction inhibits an E3 ligase

Posted on Jun 5th, 2017 - By Daniel C Scott

Nature Chemical Biology 13, 850 (2017). doi:10.1038/nchembio.2386 Authors: Daniel C Scott, Jared T Hammill, Jaeki Min, David Y Rhee, Michele Connelly, Vladislav O Sviderskiy, Deepak Bhasin, Yizhe Chen, Su-Sien Ong, Sergio C Chai, Asli N Goktug, Guochang Huang, Julie K Monda, Jonathan Low, Ho Shin Kim, Joao A Paulo, Joe R Cannon, Anang A Shelat, Taosheng Chen, Ian R Kelsall, Arno F Alpi, Vishwajeeth Pagala, Xusheng Wang, Junmin Peng, Bhuvanesh Singh, J Wade Harper, Brenda A…

Continue Reading

Activity-based probes for functional interrogation of retaining β-glucuronidases

Posted on Jun 5th, 2017 - By Liang Wu

Nature Chemical Biology 13, 867 (2017). doi:10.1038/nchembio.2395 Authors: Liang Wu, Jianbing Jiang, Yi Jin, Wouter W Kallemeijn, Chi-Lin Kuo, Marta Artola, Wei Dai, Cas van Elk, Marco van Eijk, Gijsbert A van der Marel, Jeroen D C Codée, Bogdan I Florea, Johannes M F G Aerts, Herman S Overkleeft & Gideon J…

Continue Reading

Parallel evolution of non-homologous isofunctional enzymes in methionine biosynthesis

Posted on Jun 5th, 2017 - By Karine Bastard

Nature Chemical Biology 13, 858 (2017). doi:10.1038/nchembio.2397 Authors: Karine Bastard, Alain Perret, Aline Mariage, Thomas Bessonnet, Agnès Pinet-Turpault, Jean-Louis Petit, Ekaterina Darii, Pascal Bazire, Carine Vergne-Vaxelaire, Clémence Brewee, Adrien Debard, Virginie Pellouin, Marielle Besnard-Gonnet, François Artiguenave, Claudine Médigue, David Vallenet, Antoine Danchin, Anne Zaparucha, Jean Weissenbach, Marcel Salanoubat & Véronique de…

Continue Reading

Autocatalytic backbone N-methylation in a family of ribosomal peptide natural products

Posted on Jun 5th, 2017 - By Niels S van der Velden

Nature Chemical Biology 13, 833 (2017). doi:10.1038/nchembio.2393 Authors: Niels S van der Velden, Noemi Kälin, Maximilian J Helf, Jörn Piel, Michael F Freeman & Markus Künzler Peptide backbone N-methylation, as seen in cyclosporin A, has been considered to be exclusive to nonribosomal peptides. We have identified the first post-translationally modified peptide or protein harboring internal α-N-methylations through discovery of the genetic locus for the…

Continue Reading

Functional selectivity of GPCR-directed drug action through location bias

Posted on May 29th, 2017 - By Roshanak Irannejad

Nature Chemical Biology 13, 799 (2017). doi:10.1038/nchembio.2389 Authors: Roshanak Irannejad, Veronica Pessino, Delphine Mika, Bo Huang, Philip B Wedegaertner, Marco Conti & Mark von…

Continue Reading

Targeting S-adenosylmethionine biosynthesis with a novel allosteric inhibitor of Mat2A

Posted on May 29th, 2017 - By Casey L Quinlan

Nature Chemical Biology 13, 785 (2017). doi:10.1038/nchembio.2384 Authors: Casey L Quinlan, Stephen E Kaiser, Ben Bolaños, Dawn Nowlin, Rita Grantner, Shannon Karlicek-Bryant, Jun Li Feng, Stephen Jenkinson, Kevin Freeman-Cook, Stephen G Dann, Xiaoli Wang, Peter A Wells, Valeria R Fantin, Al E Stewart & Stephan K…

Continue Reading

Structural basis for high-affinity fluorophore binding and activation by RNA Mango

Posted on May 29th, 2017 - By Robert J Trachman

Nature Chemical Biology 13, 807 (2017). doi:10.1038/nchembio.2392 Authors: Robert J Trachman, Natalia A Demeshkina, Matthew W L Lau, Shanker Shyam S Panchapakesan, Sunny C Y Jeng, Peter J Unrau & Adrian R…

Continue Reading

Lipid II overproduction allows direct assay of transpeptidase inhibition by β-lactams

Posted on May 29th, 2017 - By Yuan Qiao

Nature Chemical Biology 13, 793 (2017). doi:10.1038/nchembio.2388 Authors: Yuan Qiao, Veerasak Srisuknimit, Frederick Rubino, Kaitlin Schaefer, Natividad Ruiz, Suzanne Walker & Daniel…

Continue Reading

Structural and functional characterization of the hydrogenase-maturation HydF protein

Posted on May 29th, 2017 - By Giorgio Caserta

Nature Chemical Biology 13, 779 (2017). doi:10.1038/nchembio.2385 Authors: Giorgio Caserta, Ludovic Pecqueur, Agnieszka Adamska-Venkatesh, Cecilia Papini, Souvik Roy, Vincent Artero, Mohamed Atta, Edward Reijerse, Wolfgang Lubitz & Marc…

Continue Reading

Engineering protein stability with atomic precision in a monomeric miniprotein

Posted on May 22nd, 2017 - By Emily G Baker

Nature Chemical Biology 13, 764 (2017). doi:10.1038/nchembio.2380 Authors: Emily G Baker, Christopher Williams, Kieran L Hudson, Gail J Bartlett, Jack W Heal, Kathryn L Porter Goff, Richard B Sessions, Matthew P Crump & Derek N…

Continue Reading

A combinatorial screen of the CLOUD uncovers a synergy targeting the androgen receptor

Posted on May 22nd, 2017 - By Marco P Licciardello

Nature Chemical Biology 13, 771 (2017). doi:10.1038/nchembio.2382 Authors: Marco P Licciardello, Anna Ringler, Patrick Markt, Freya Klepsch, Charles-Hugues Lardeau, Sara Sdelci, Erika Schirghuber, André C Müller, Michael Caldera, Anja Wagner, Rebecca Herzog, Thomas Penz, Michael Schuster, Bernd Boidol, Gerhard Dürnberger, Yasin Folkvaljon, Pär Stattin, Vladimir Ivanov, Jacques Colinge, Christoph Bock, Klaus Kratochwill, Jörg Menche, Keiryn L Bennett &…

Continue Reading

Recognition of EGF-like domains by the Notch-modifying O-fucosyltransferase POFUT1

Posted on May 22nd, 2017 - By Zhijie Li

Nature Chemical Biology 13, 757 (2017). doi:10.1038/nchembio.2381 Authors: Zhijie Li, Kristina Han, John E Pak, Malathy Satkunarajah, Dongxia Zhou & James M…

Continue Reading

Engineering RGB color vision into Escherichia coli

Posted on May 22nd, 2017 - By Jesus Fernandez-Rodriguez

Nature Chemical Biology 13, 706 (2017). doi:10.1038/nchembio.2390 Authors: Jesus Fernandez-Rodriguez, Felix Moser, Miryoung Song & Christopher A Voigt Optogenetic tools use colored light to rapidly control gene expression in space and time. We designed a genetically encoded system that gives Escherichia coli the ability to distinguish between red, green, and blue (RGB) light and respond by changing gene expression. We use this system to produce 'color photographs' on…

Continue Reading

Enzymology: Radical ring resizing

Posted on May 17th, 2017 - By Caitlin Deane

Nature Chemical Biology 13, 569 (2017). doi:10.1038/nchembio.2399 Author: Caitlin…

Continue Reading

Synthetic biology: Return to sender

Posted on May 17th, 2017 - By Grant Miura

Nature Chemical Biology 13, 569 (2017). doi:10.1038/nchembio.2398 Author: Grant…

Continue Reading

Plant development: Get lit on steroids

Posted on May 17th, 2017 - By Mirella Bucci

Nature Chemical Biology 13, 569 (2017). doi:10.1038/nchembio.2401 Author: Mirella…

Continue Reading

Signal Transduction: Notch catches a Jagged edge

Posted on May 17th, 2017 - By Stephen C Blacklow

Nature Chemical Biology 13, 570 (2017). doi:10.1038/nchembio.2379 Author: Stephen C Blacklow Notch signaling is an essential cell–cell communication pathway that influences numerous cell fate decisions during development. Structural and biochemical studies of a Notch–Jagged complex dramatically advance current understanding of ligand recognition, and reveal evidence of catch-bond behavior in the…

Continue Reading

Erratum: A Vibrio cholerae autoinducer–receptor pair that controls biofilm formation

Posted on May 17th, 2017 - By Kai Papenfort

Nature Chemical Biology 13, 691 (2017). doi:10.1038/nchembio0617-691a Author: Kai Papenfort, Justin E Silpe, Kelsey R Schramma, Jian-Ping Cong, Mohammad R Seyedsayamdost & Bonnie L…

Continue Reading

Optogenetics: Switching with red and blue

Posted on May 17th, 2017 - By Fuun Kawano

Nature Chemical Biology 13, 573 (2017). doi:10.1038/nchembio.2387 Authors: Fuun Kawano, Fan Shi & Masayuki Yazawa Multiple optogenetic technologies are required to control biological activity simultaneously with different colors of light. Optimizing a near-infrared-induced heterodimerization system, which can be combined with blue-light-controlled domains, enables precise spatiotemporal control of target molecules in live mammalian…

Continue Reading

Small-molecule pheromones and hormones controlling nematode development

Posted on May 17th, 2017 - By Rebecca A Butcher

Nature Chemical Biology 13, 577 (2017). doi:10.1038/nchembio.2356 Author: Rebecca A…

Continue Reading

Illuminating developmental biology through photochemistry

Posted on May 17th, 2017 - By Lukasz Kowalik

Nature Chemical Biology 13, 587 (2017). doi:10.1038/nchembio.2369 Authors: Lukasz Kowalik & James K…

Continue Reading

The perception of strigolactones in vascular plants

Posted on May 17th, 2017 - By Shelley Lumba

Nature Chemical Biology 13, 599 (2017). doi:10.1038/nchembio.2340 Authors: Shelley Lumba, Duncan Holbrook-Smith & Peter…

Continue Reading

Erratum: Full antagonism of the estrogen receptor without a prototypical ligand side chain

Posted on May 17th, 2017 - By Sathish Srinivasan

Nature Chemical Biology 13, 691 (2017). doi:10.1038/nchembio0617-691b Author: Sathish Srinivasan, Jerome C Nwachukwu, Nelson E Bruno, Venkatasubramanian Dharmarajan, Devrishi Goswami, Irida Kastrati, Scott Novick, Jason Nowak, Valerie Cavett, Hai-Bing Zhou, Nittaya Boonmuen, Yuechao Zhao, Jian Min, Jonna Frasor, Benita S Katzenellenbogen, Patrick R Griffin, John A Katzenellenbogen & Kendall W…

Continue Reading

Shaping embryonic development

Nature Chemical Biology 13, 559 (2017). doi:10.1038/nchembio.2403 The growing intersection between chemical tools and principles and developmental biology is providing new insights into the molecular-level details of developmental…

Continue Reading

Small-molecule phenotypic screening with stem cells

Posted on May 17th, 2017 - By Andrei Ursu

Nature Chemical Biology 13, 560 (2017). doi:10.1038/nchembio.2383 Authors: Andrei Ursu, Hans R Schöler & Herbert Waldmann To fully leverage the potential of human-induced pluripotent stem cells (hiPSCs), improved and standardized reprogramming methods and large-scale collections of hiPSC lines are needed, and the stem cell community must embrace chemical biology methodology for target identification and…

Continue Reading

Corrigendum: Full antagonism of the estrogen receptor without a prototypical ligand side chain

Posted on May 17th, 2017 - By Sathish Srinivasan

Nature Chemical Biology 13, 691 (2017). doi:10.1038/nchembio0617-691c Author: Sathish Srinivasan, Jerome C Nwachukwu, Nelson E Bruno, Venkatasubramanian Dharmarajan, Devrishi Goswami, Irida Kastrati, Scott Novick, Jason Nowak, Valerie Cavett, Hai-Bing Zhou, Nittaya Boonmuen, Yuechao Zhao, Jian Min, Jonna Frasor, Benita S Katzenellenbogen, Patrick R Griffin, John A Katzenellenbogen & Kendall W…

Continue Reading

Infectious disease: A lethal sugar fix

Posted on May 17th, 2017 - By Alison Farrell

Nature Chemical Biology 13, 569 (2017). doi:10.1038/nchembio.2400 Author: Alison…

Continue Reading

Unraveling cell-to-cell signaling networks with chemical biology

Posted on May 17th, 2017 - By Zev J Gartner

Nature Chemical Biology 13, 564 (2017). doi:10.1038/nchembio.2391 Authors: Zev J Gartner, Jennifer A Prescher & Luke D Lavis Cell-to-cell signaling networks, although poorly understood, guide tissue development, regulate tissue function and may become dysregulated in disease. Chemical biologists can develop the next generation of tools to untangle these complex and dynamic networks of interacting…

Continue Reading

β-Lactone formation during product release from a nonribosomal peptide synthetase

Posted on May 15th, 2017 - By Jason E Schaffer

Nature Chemical Biology 13, 737 (2017). doi:10.1038/nchembio.2374 Authors: Jason E Schaffer, Margaret R Reck, Neha K Prasad & Timothy A…

Continue Reading

Developing Spindlin1 small-molecule inhibitors by using protein microarrays

Posted on May 15th, 2017 - By Narkhyun Bae

Nature Chemical Biology 13, 750 (2017). doi:10.1038/nchembio.2377 Authors: Narkhyun Bae, Monica Viviano, Xiaonan Su, Jie Lv, Donghang Cheng, Cari Sagum, Sabrina Castellano, Xue Bai, Claire Johnson, Mahmoud Ibrahim Khalil, Jianjun Shen, Kaifu Chen, Haitao Li, Gianluca Sbardella & Mark T…

Continue Reading

A conserved threonine prevents self-intoxication of enoyl-thioester reductases

Posted on May 15th, 2017 - By Raoul G Rosenthal

Nature Chemical Biology 13, 745 (2017). doi:10.1038/nchembio.2375 Authors: Raoul G Rosenthal, Bastian Vögeli, Tristan Wagner, Seigo Shima & Tobias J…

Continue Reading

Antibiotic resistance: Blocking tetracycline destruction

Posted on May 8th, 2017 - By Sonja Petkovic

Nature Chemical Biology 13, 694 (2017). doi:10.1038/nchembio.2396 Authors: Sonja Petkovic & Winfried Hinrichs Enzymology and structural and functional characterization of some FAD-dependent monooxygenases provide insights into degradation of tetracycline antibiotics, but also show unexpected features of substrate recognition, reaction mechanism, and competitive…

Continue Reading

Membrane curvature regulates ligand-specific membrane sorting of GPCRs in living cells

Posted on May 8th, 2017 - By Kadla R Rosholm

Nature Chemical Biology 13, 724 (2017). doi:10.1038/nchembio.2372 Authors: Kadla R Rosholm, Natascha Leijnse, Anna Mantsiou, Vadym Tkach, Søren L Pedersen, Volker F Wirth, Lene B Oddershede, Knud J Jensen, Karen L Martinez, Nikos S Hatzakis, Poul Martin Bendix, Andrew Callan-Jones & Dimitrios…

Continue Reading

Plasticity, dynamics, and inhibition of emerging tetracycline resistance enzymes

Posted on May 8th, 2017 - By Jooyoung Park

Nature Chemical Biology 13, 730 (2017). doi:10.1038/nchembio.2376 Authors: Jooyoung Park, Andrew J Gasparrini, Margaret R Reck, Chanez T Symister, Jennifer L Elliott, Joseph P Vogel, Timothy A Wencewicz, Gautam Dantas & Niraj H…

Continue Reading

Computational design of ligand-binding membrane receptors with high selectivity

Posted on May 1st, 2017 - By Xiang Feng

Nature Chemical Biology 13, 715 (2017). doi:10.1038/nchembio.2371 Authors: Xiang Feng, Joaquin Ambia, Kuang-Yui M Chen, Melvin Young & Patrick…

Continue Reading

Thiolutin is a zinc chelator that inhibits the Rpn11 and other JAMM metalloproteases

Posted on May 1st, 2017 - By Linda Lauinger

Nature Chemical Biology 13, 709 (2017). doi:10.1038/nchembio.2370 Authors: Linda Lauinger, Jing Li, Anton Shostak, Ibrahim Avi Cemel, Nati Ha, Yaru Zhang, Philipp E Merkl, Simon Obermeyer, Nicolas Stankovic-Valentin, Tobias Schafmeier, Walter J Wever, Albert A Bowers, Kyle P Carter, Amy E Palmer, Herbert Tschochner, Frauke Melchior, Raymond J Deshaies, Michael Brunner & Axel…

Continue Reading

Protein degradation: DCAFinating splicing

Posted on Apr 24th, 2017 - By Georg E Winter

Nature Chemical Biology 13, 575 (2017). doi:10.1038/nchembio.2378 Author: Georg E Winter Chemical control of protein homeostasis and induction of protein destabilization are emerging therapeutic strategies. Two recent studies identify a set of sulfonamides that can modulate the CRL4DCAF15 E3 ligase complex to target the splicing factor RBM39 for proteasomal…

Continue Reading

Global survey of the immunomodulatory potential of common drugs

Posted on Apr 24th, 2017 - By Gregory I Vladimer

Nature Chemical Biology 13, 681 (2017). doi:10.1038/nchembio.2360 Authors: Gregory I Vladimer, Berend Snijder, Nikolaus Krall, Johannes W Bigenzahn, Kilian V M Huber, Charles-Hugues Lardeau, Kumar Sanjiv, Anna Ringler, Ulrika Warpman Berglund, Monika Sabler, Oscar Lopez de la Fuente, Paul Knöbl, Stefan Kubicek, Thomas Helleday, Ulrich Jäger & Giulio…

Continue Reading

Selective degradation of splicing factor CAPERα by anticancer sulfonamides

Posted on Apr 24th, 2017 - By Taisuke Uehara

Nature Chemical Biology 13, 675 (2017). doi:10.1038/nchembio.2363 Authors: Taisuke Uehara, Yukinori Minoshima, Koji Sagane, Naoko Hata Sugi, Kaoru Ogawa Mitsuhashi, Noboru Yamamoto, Hiroshi Kamiyama, Kentaro Takahashi, Yoshihiko Kotake, Mai Uesugi, Akira Yokoi, Atsushi Inoue, Taku Yoshida, Miyuki Mabuchi, Akito Tanaka & Takashi…

Continue Reading

Immunology: Mind the immuno-connection gap

Posted on Apr 24th, 2017 - By Wolfgang Link

Nature Chemical Biology 13, 572 (2017). doi:10.1038/nchembio.2373 Author: Wolfgang Link Biologic drugs that modulate the immune system have revolutionized the therapeutic landscape for several selected cancer types. A new study reports an image-based assay system to monitor cell–cell interactions, identifying small-molecule compounds with immunomodulatory…

Continue Reading

Using the pimeloyl-CoA synthetase adenylation fold to synthesize fatty acid thioesters

Posted on Apr 17th, 2017 - By Menglu Wang

Nature Chemical Biology 13, 660 (2017). doi:10.1038/nchembio.2361 Authors: Menglu Wang, Lucile Moynié, Peter J Harrison, Van Kelly, Andrew Piper, James H Naismith & Dominic J…

Continue Reading

The pimeloyl-CoA synthetase BioW defines a new fold for adenylate-forming enzymes

Posted on Apr 17th, 2017 - By Paola Estrada

Nature Chemical Biology 13, 668 (2017). doi:10.1038/nchembio.2359 Authors: Paola Estrada, Miglena Manandhar, Shi-Hui Dong, Jaigeeth Deveryshetty, Vinayak Agarwal, John E Cronan & Satish K…

Continue Reading

Optogenetics: Lighting up kinases

Posted on Apr 13th, 2017 - By Grant Miura

Nature Chemical Biology 13, 451 (2017). doi:10.1038/nchembio.2365 Author: Grant…

Continue Reading

Bacterial Membranes: Tear down this wall

Posted on Apr 13th, 2017 - By Caitlin Deane

Nature Chemical Biology 13, 451 (2017). doi:10.1038/nchembio.2366 Author: Caitlin…

Continue Reading

Microbiology: A new language for small talk

Posted on Apr 13th, 2017 - By Yi-Ming Shi

Nature Chemical Biology 13, 453 (2017). doi:10.1038/nchembio.2362 Authors: Yi-Ming Shi & Helge B Bode A new signal–receptor pair involved in regulating biofilm formation and virulence was detected in Vibrio cholerae. Both the signal and the transcription factor belong to common classes of natural products and receptor proteins, suggesting widespread importance of related systems in…

Continue Reading

Intrinsic Disorder: Fuzzy fast feedback

Posted on Apr 13th, 2017 - By Mirella Bucci

Nature Chemical Biology 13, 451 (2017). doi:10.1038/nchembio.2367 Author: Mirella…

Continue Reading

Targeted protein degradation: You can glue it too!

Posted on Apr 13th, 2017 - By Michal J Walczak

Nature Chemical Biology 13, 452 (2017). doi:10.1038/nchembio.2355 Authors: Michal J Walczak, Georg Petzold & Nicolas H Thomä Proteolysis-targeting chimera (PROTACs) are synthetic molecules that recruit neo-substrate proteins to a ubiquitin ligase for ubiquitination and subsequent degradation. Structural insight into the VHL–MZ1–BRD4 complex reveals how the rationally designed MZ1–PROTAC molecule mediates degradation of an unnatural protein…

Continue Reading

Anti-Bacterials: Out-SMARting drug resistance

Posted on Apr 13th, 2017 - By Alison Farrell

Nature Chemical Biology 13, 451 (2017). doi:10.1038/nchembio.2368 Author: Alison…

Continue Reading

The chemical basis for electrical signaling

Posted on Apr 13th, 2017 - By William A Catterall

Nature Chemical Biology 13, 455 (2017). doi:10.1038/nchembio.2353 Authors: William A Catterall, Goragot Wisedchaisri & Ning…

Continue Reading

CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters

Posted on Apr 10th, 2017 - By Mingzi M Zhang

Nature Chemical Biology 13, 607 (2017). doi:10.1038/nchembio.2341 Authors: Mingzi M Zhang, Fong Tian Wong, Yajie Wang, Shangwen Luo, Yee Hwee Lim, Elena Heng, Wan Lin Yeo, Ryan E Cobb, Behnam Enghiad, Ee Lui Ang & Huimin Zhao Here we report an efficient CRISPR–Cas9 knock-in strategy to activate silent biosynthetic gene clusters (BGCs) in streptomycetes. We applied this one-step strategy to activate multiple BGCs of different classes in five Streptomyces species and…

Continue Reading

Mechanistic insights into energy conservation by flavin-based electron bifurcation

Posted on Apr 10th, 2017 - By Carolyn E Lubner

Nature Chemical Biology 13, 655 (2017). doi:10.1038/nchembio.2348 Authors: Carolyn E Lubner, David P Jennings, David W Mulder, Gerrit J Schut, Oleg A Zadvornyy, John P Hoben, Monika Tokmina-Lukaszewska, Luke Berry, Diep M Nguyen, Gina L Lipscomb, Brian Bothner, Anne K Jones, Anne-Frances Miller, Paul W King, Michael W W Adams & John W…

Continue Reading

Phosphorylated glycosphingolipids essential for cholesterol mobilization in Caenorhabditis elegans

Posted on Apr 3rd, 2017 - By Sebastian Boland

Nature Chemical Biology 13, 647 (2017). doi:10.1038/nchembio.2347 Authors: Sebastian Boland, Ulrike Schmidt, Vyacheslav Zagoriy, Julio L Sampaio, Raphael F Fritsche, Regina Czerwonka, Tilo Lübken, Jakob Reimann, Sider Penkov, Hans-Joachim Knölker & Teymuras V…

Continue Reading

AtaT blocks translation initiation by N-acetylation of the initiator tRNAfMet

Posted on Apr 3rd, 2017 - By Dukas Jurėnas

Nature Chemical Biology 13, 640 (2017). doi:10.1038/nchembio.2346 Authors: Dukas Jurėnas, Sneha Chatterjee, Albert Konijnenberg, Frank Sobott, Louis Droogmans, Abel Garcia-Pino & Laurence Van…

Continue Reading

Structural and functional insight into human O-GlcNAcase

Posted on Mar 27th, 2017 - By Christian Roth

Nature Chemical Biology 13, 610 (2017). doi:10.1038/nchembio.2358 Authors: Christian Roth, Sherry Chan, Wendy A Offen, Glyn R Hemsworth, Lianne I Willems, Dustin T King, Vimal Varghese, Robert Britton, David J Vocadlo & Gideon J Davies O-GlcNAc hydrolase (OGA) removes O-linked N-acetylglucosamine (O-GlcNAc) from a myriad of nucleocytoplasmic proteins. Through co-expression and assembly of OGA fragments, we determined the three-dimensional structure of human OGA, revealing an…

Continue Reading

Insights into activity and inhibition from the crystal structure of human O-GlcNAcase

Posted on Mar 27th, 2017 - By Nathaniel L Elsen

Nature Chemical Biology 13, 613 (2017). doi:10.1038/nchembio.2357 Authors: Nathaniel L Elsen, Sangita B Patel, Rachael E Ford, Dawn L Hall, Fred Hess, Hari Kandula, Maria Kornienko, John Reid, Harold Selnick, Jennifer M Shipman, Sujata Sharma, Kevin J Lumb, Stephen M Soisson & Daniel J Klein O-GlcNAc hydrolase (OGA) catalyzes removal of βα-linked N-acetyl-D-glucosamine from serine and threonine residues. We report crystal structures of Homo sapiens OGA catalytic…

Continue Reading

Diabetes reversal by inhibition of the low-molecular-weight tyrosine phosphatase

Posted on Mar 27th, 2017 - By Stephanie M Stanford

Nature Chemical Biology 13, 624 (2017). doi:10.1038/nchembio.2344 Authors: Stephanie M Stanford, Alexander E Aleshin, Vida Zhang, Robert J Ardecky, Michael P Hedrick, Jiwen Zou, Santhi R Ganji, Matthew R Bliss, Fusayo Yamamoto, Andrey A Bobkov, Janna Kiselar, Yingge Liu, Gregory W Cadwell, Shilpi Khare, Jinghua Yu, Antonio Barquilla, Thomas D Y Chung, Tomas Mustelin, Simon Schenk, Laurie A Bankston, Robert C Liddington, Anthony B Pinkerton & Nunzio…

Continue Reading