Enzymology: Radical ring resizing

Posted on May 17th, 2017 - By Caitlin Deane

Nature Chemical Biology 13, 569 (2017). doi:10.1038/nchembio.2399 Author: Caitlin…

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Synthetic biology: Return to sender

Posted on May 17th, 2017 - By Grant Miura

Nature Chemical Biology 13, 569 (2017). doi:10.1038/nchembio.2398 Author: Grant…

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Plant development: Get lit on steroids

Posted on May 17th, 2017 - By Mirella Bucci

Nature Chemical Biology 13, 569 (2017). doi:10.1038/nchembio.2401 Author: Mirella…

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Signal Transduction: Notch catches a Jagged edge

Posted on May 17th, 2017 - By Stephen C Blacklow

Nature Chemical Biology 13, 570 (2017). doi:10.1038/nchembio.2379 Author: Stephen C Blacklow Notch signaling is an essential cell–cell communication pathway that influences numerous cell fate decisions during development. Structural and biochemical studies of a Notch–Jagged complex dramatically advance current understanding of ligand recognition, and reveal evidence of catch-bond behavior in the…

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Erratum: A Vibrio cholerae autoinducer–receptor pair that controls biofilm formation

Posted on May 17th, 2017 - By Kai Papenfort

Nature Chemical Biology 13, 691 (2017). doi:10.1038/nchembio0617-691a Author: Kai Papenfort, Justin E Silpe, Kelsey R Schramma, Jian-Ping Cong, Mohammad R Seyedsayamdost & Bonnie L…

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Optogenetics: Switching with red and blue

Posted on May 17th, 2017 - By Fuun Kawano

Nature Chemical Biology 13, 573 (2017). doi:10.1038/nchembio.2387 Authors: Fuun Kawano, Fan Shi & Masayuki Yazawa Multiple optogenetic technologies are required to control biological activity simultaneously with different colors of light. Optimizing a near-infrared-induced heterodimerization system, which can be combined with blue-light-controlled domains, enables precise spatiotemporal control of target molecules in live mammalian…

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Small-molecule pheromones and hormones controlling nematode development

Posted on May 17th, 2017 - By Rebecca A Butcher

Nature Chemical Biology 13, 577 (2017). doi:10.1038/nchembio.2356 Author: Rebecca A…

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Illuminating developmental biology through photochemistry

Posted on May 17th, 2017 - By Lukasz Kowalik

Nature Chemical Biology 13, 587 (2017). doi:10.1038/nchembio.2369 Authors: Lukasz Kowalik & James K…

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The perception of strigolactones in vascular plants

Posted on May 17th, 2017 - By Shelley Lumba

Nature Chemical Biology 13, 599 (2017). doi:10.1038/nchembio.2340 Authors: Shelley Lumba, Duncan Holbrook-Smith & Peter…

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Erratum: Full antagonism of the estrogen receptor without a prototypical ligand side chain

Posted on May 17th, 2017 - By Sathish Srinivasan

Nature Chemical Biology 13, 691 (2017). doi:10.1038/nchembio0617-691b Author: Sathish Srinivasan, Jerome C Nwachukwu, Nelson E Bruno, Venkatasubramanian Dharmarajan, Devrishi Goswami, Irida Kastrati, Scott Novick, Jason Nowak, Valerie Cavett, Hai-Bing Zhou, Nittaya Boonmuen, Yuechao Zhao, Jian Min, Jonna Frasor, Benita S Katzenellenbogen, Patrick R Griffin, John A Katzenellenbogen & Kendall W…

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Shaping embryonic development

Nature Chemical Biology 13, 559 (2017). doi:10.1038/nchembio.2403 The growing intersection between chemical tools and principles and developmental biology is providing new insights into the molecular-level details of developmental…

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Small-molecule phenotypic screening with stem cells

Posted on May 17th, 2017 - By Andrei Ursu

Nature Chemical Biology 13, 560 (2017). doi:10.1038/nchembio.2383 Authors: Andrei Ursu, Hans R Schöler & Herbert Waldmann To fully leverage the potential of human-induced pluripotent stem cells (hiPSCs), improved and standardized reprogramming methods and large-scale collections of hiPSC lines are needed, and the stem cell community must embrace chemical biology methodology for target identification and…

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Corrigendum: Full antagonism of the estrogen receptor without a prototypical ligand side chain

Posted on May 17th, 2017 - By Sathish Srinivasan

Nature Chemical Biology 13, 691 (2017). doi:10.1038/nchembio0617-691c Author: Sathish Srinivasan, Jerome C Nwachukwu, Nelson E Bruno, Venkatasubramanian Dharmarajan, Devrishi Goswami, Irida Kastrati, Scott Novick, Jason Nowak, Valerie Cavett, Hai-Bing Zhou, Nittaya Boonmuen, Yuechao Zhao, Jian Min, Jonna Frasor, Benita S Katzenellenbogen, Patrick R Griffin, John A Katzenellenbogen & Kendall W…

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Infectious disease: A lethal sugar fix

Posted on May 17th, 2017 - By Alison Farrell

Nature Chemical Biology 13, 569 (2017). doi:10.1038/nchembio.2400 Author: Alison…

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Unraveling cell-to-cell signaling networks with chemical biology

Posted on May 17th, 2017 - By Zev J Gartner

Nature Chemical Biology 13, 564 (2017). doi:10.1038/nchembio.2391 Authors: Zev J Gartner, Jennifer A Prescher & Luke D Lavis Cell-to-cell signaling networks, although poorly understood, guide tissue development, regulate tissue function and may become dysregulated in disease. Chemical biologists can develop the next generation of tools to untangle these complex and dynamic networks of interacting…

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Protein degradation: DCAFinating splicing

Posted on Apr 24th, 2017 - By Georg E Winter

Nature Chemical Biology 13, 575 (2017). doi:10.1038/nchembio.2378 Author: Georg E Winter Chemical control of protein homeostasis and induction of protein destabilization are emerging therapeutic strategies. Two recent studies identify a set of sulfonamides that can modulate the CRL4DCAF15 E3 ligase complex to target the splicing factor RBM39 for proteasomal…

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Global survey of the immunomodulatory potential of common drugs

Posted on Apr 24th, 2017 - By Gregory I Vladimer

Nature Chemical Biology 13, 681 (2017). doi:10.1038/nchembio.2360 Authors: Gregory I Vladimer, Berend Snijder, Nikolaus Krall, Johannes W Bigenzahn, Kilian V M Huber, Charles-Hugues Lardeau, Kumar Sanjiv, Anna Ringler, Ulrika Warpman Berglund, Monika Sabler, Oscar Lopez de la Fuente, Paul Knöbl, Stefan Kubicek, Thomas Helleday, Ulrich Jäger & Giulio…

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Selective degradation of splicing factor CAPERα by anticancer sulfonamides

Posted on Apr 24th, 2017 - By Taisuke Uehara

Nature Chemical Biology 13, 675 (2017). doi:10.1038/nchembio.2363 Authors: Taisuke Uehara, Yukinori Minoshima, Koji Sagane, Naoko Hata Sugi, Kaoru Ogawa Mitsuhashi, Noboru Yamamoto, Hiroshi Kamiyama, Kentaro Takahashi, Yoshihiko Kotake, Mai Uesugi, Akira Yokoi, Atsushi Inoue, Taku Yoshida, Miyuki Mabuchi, Akito Tanaka & Takashi…

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Immunology: Mind the immuno-connection gap

Posted on Apr 24th, 2017 - By Wolfgang Link

Nature Chemical Biology 13, 572 (2017). doi:10.1038/nchembio.2373 Author: Wolfgang Link Biologic drugs that modulate the immune system have revolutionized the therapeutic landscape for several selected cancer types. A new study reports an image-based assay system to monitor cell–cell interactions, identifying small-molecule compounds with immunomodulatory…

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Using the pimeloyl-CoA synthetase adenylation fold to synthesize fatty acid thioesters

Posted on Apr 17th, 2017 - By Menglu Wang

Nature Chemical Biology 13, 660 (2017). doi:10.1038/nchembio.2361 Authors: Menglu Wang, Lucile Moynié, Peter J Harrison, Van Kelly, Andrew Piper, James H Naismith & Dominic J…

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The pimeloyl-CoA synthetase BioW defines a new fold for adenylate-forming enzymes

Posted on Apr 17th, 2017 - By Paola Estrada

Nature Chemical Biology 13, 668 (2017). doi:10.1038/nchembio.2359 Authors: Paola Estrada, Miglena Manandhar, Shi-Hui Dong, Jaigeeth Deveryshetty, Vinayak Agarwal, John E Cronan & Satish K…

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Optogenetics: Lighting up kinases

Posted on Apr 13th, 2017 - By Grant Miura

Nature Chemical Biology 13, 451 (2017). doi:10.1038/nchembio.2365 Author: Grant…

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Bacterial Membranes: Tear down this wall

Posted on Apr 13th, 2017 - By Caitlin Deane

Nature Chemical Biology 13, 451 (2017). doi:10.1038/nchembio.2366 Author: Caitlin…

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Microbiology: A new language for small talk

Posted on Apr 13th, 2017 - By Yi-Ming Shi

Nature Chemical Biology 13, 453 (2017). doi:10.1038/nchembio.2362 Authors: Yi-Ming Shi & Helge B Bode A new signal–receptor pair involved in regulating biofilm formation and virulence was detected in Vibrio cholerae. Both the signal and the transcription factor belong to common classes of natural products and receptor proteins, suggesting widespread importance of related systems in…

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Intrinsic Disorder: Fuzzy fast feedback

Posted on Apr 13th, 2017 - By Mirella Bucci

Nature Chemical Biology 13, 451 (2017). doi:10.1038/nchembio.2367 Author: Mirella…

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Targeted protein degradation: You can glue it too!

Posted on Apr 13th, 2017 - By Michal J Walczak

Nature Chemical Biology 13, 452 (2017). doi:10.1038/nchembio.2355 Authors: Michal J Walczak, Georg Petzold & Nicolas H Thomä Proteolysis-targeting chimera (PROTACs) are synthetic molecules that recruit neo-substrate proteins to a ubiquitin ligase for ubiquitination and subsequent degradation. Structural insight into the VHL–MZ1–BRD4 complex reveals how the rationally designed MZ1–PROTAC molecule mediates degradation of an unnatural protein…

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Anti-Bacterials: Out-SMARting drug resistance

Posted on Apr 13th, 2017 - By Alison Farrell

Nature Chemical Biology 13, 451 (2017). doi:10.1038/nchembio.2368 Author: Alison…

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The chemical basis for electrical signaling

Posted on Apr 13th, 2017 - By William A Catterall

Nature Chemical Biology 13, 455 (2017). doi:10.1038/nchembio.2353 Authors: William A Catterall, Goragot Wisedchaisri & Ning…

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CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters

Posted on Apr 10th, 2017 - By Mingzi M Zhang

Nature Chemical Biology 13, 607 (2017). doi:10.1038/nchembio.2341 Authors: Mingzi M Zhang, Fong Tian Wong, Yajie Wang, Shangwen Luo, Yee Hwee Lim, Elena Heng, Wan Lin Yeo, Ryan E Cobb, Behnam Enghiad, Ee Lui Ang & Huimin Zhao Here we report an efficient CRISPR–Cas9 knock-in strategy to activate silent biosynthetic gene clusters (BGCs) in streptomycetes. We applied this one-step strategy to activate multiple BGCs of different classes in five Streptomyces species and…

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Mechanistic insights into energy conservation by flavin-based electron bifurcation

Posted on Apr 10th, 2017 - By Carolyn E Lubner

Nature Chemical Biology 13, 655 (2017). doi:10.1038/nchembio.2348 Authors: Carolyn E Lubner, David P Jennings, David W Mulder, Gerrit J Schut, Oleg A Zadvornyy, John P Hoben, Monika Tokmina-Lukaszewska, Luke Berry, Diep M Nguyen, Gina L Lipscomb, Brian Bothner, Anne K Jones, Anne-Frances Miller, Paul W King, Michael W W Adams & John W…

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Phosphorylated glycosphingolipids essential for cholesterol mobilization in Caenorhabditis elegans

Posted on Apr 3rd, 2017 - By Sebastian Boland

Nature Chemical Biology 13, 647 (2017). doi:10.1038/nchembio.2347 Authors: Sebastian Boland, Ulrike Schmidt, Vyacheslav Zagoriy, Julio L Sampaio, Raphael F Fritsche, Regina Czerwonka, Tilo Lübken, Jakob Reimann, Sider Penkov, Hans-Joachim Knölker & Teymuras V…

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AtaT blocks translation initiation by N-acetylation of the initiator tRNAfMet

Posted on Apr 3rd, 2017 - By Dukas Jurėnas

Nature Chemical Biology 13, 640 (2017). doi:10.1038/nchembio.2346 Authors: Dukas Jurėnas, Sneha Chatterjee, Albert Konijnenberg, Frank Sobott, Louis Droogmans, Abel Garcia-Pino & Laurence Van…

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Structural and functional insight into human O-GlcNAcase

Posted on Mar 27th, 2017 - By Christian Roth

Nature Chemical Biology 13, 610 (2017). doi:10.1038/nchembio.2358 Authors: Christian Roth, Sherry Chan, Wendy A Offen, Glyn R Hemsworth, Lianne I Willems, Dustin T King, Vimal Varghese, Robert Britton, David J Vocadlo & Gideon J Davies O-GlcNAc hydrolase (OGA) removes O-linked N-acetylglucosamine (O-GlcNAc) from a myriad of nucleocytoplasmic proteins. Through co-expression and assembly of OGA fragments, we determined the three-dimensional structure of human OGA, revealing an…

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Insights into activity and inhibition from the crystal structure of human O-GlcNAcase

Posted on Mar 27th, 2017 - By Nathaniel L Elsen

Nature Chemical Biology 13, 613 (2017). doi:10.1038/nchembio.2357 Authors: Nathaniel L Elsen, Sangita B Patel, Rachael E Ford, Dawn L Hall, Fred Hess, Hari Kandula, Maria Kornienko, John Reid, Harold Selnick, Jennifer M Shipman, Sujata Sharma, Kevin J Lumb, Stephen M Soisson & Daniel J Klein O-GlcNAc hydrolase (OGA) catalyzes removal of βα-linked N-acetyl-D-glucosamine from serine and threonine residues. We report crystal structures of Homo sapiens OGA catalytic…

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Diabetes reversal by inhibition of the low-molecular-weight tyrosine phosphatase

Posted on Mar 27th, 2017 - By Stephanie M Stanford

Nature Chemical Biology 13, 624 (2017). doi:10.1038/nchembio.2344 Authors: Stephanie M Stanford, Alexander E Aleshin, Vida Zhang, Robert J Ardecky, Michael P Hedrick, Jiwen Zou, Santhi R Ganji, Matthew R Bliss, Fusayo Yamamoto, Andrey A Bobkov, Janna Kiselar, Yingge Liu, Gregory W Cadwell, Shilpi Khare, Jinghua Yu, Antonio Barquilla, Thomas D Y Chung, Tomas Mustelin, Simon Schenk, Laurie A Bankston, Robert C Liddington, Anthony B Pinkerton & Nunzio…

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Chemical screening identifies ATM as a target for alleviating senescence

Posted on Mar 27th, 2017 - By Hyun Tae Kang

Nature Chemical Biology 13, 616 (2017). doi:10.1038/nchembio.2342 Authors: Hyun Tae Kang, Joon Tae Park, Kobong Choi, Yongsub Kim, Hyo Jei Claudia Choi, Chul Won Jung, Young-Sam Lee & Sang Chul…

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Near-infrared optogenetic pair for protein regulation and spectral multiplexing

Posted on Mar 27th, 2017 - By Taras A Redchuk

Nature Chemical Biology 13, 633 (2017). doi:10.1038/nchembio.2343 Authors: Taras A Redchuk, Evgeniya S Omelina, Konstantin G Chernov & Vladislav V…

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Drug discovery: Uncoupling coupled transport

Posted on Mar 22nd, 2017 - By Mirella Bucci

Nature Chemical Biology 13, 343 (2017). doi:10.1038/nchembio.2351 Author: Mirella…

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Development: Marking the transition

Posted on Mar 22nd, 2017 - By Grant Miura

Nature Chemical Biology 13, 343 (2017). doi:10.1038/nchembio.2349 Author: Grant…

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Fatty acid synthases: Re-engineering biofactories

Posted on Mar 22nd, 2017 - By Timm Maier

Nature Chemical Biology 13, 344 (2017). doi:10.1038/nchembio.2338 Author: Timm Maier Systematically modifying biological assembly lines for the synthesis of novel products remains a challenge. Structural insights and computational modeling have now paved the way for efficient redesigns of giant fatty acid…

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Bioconjugation: Methionine’s time to shine

Posted on Mar 22nd, 2017 - By Caitlin Deane

Nature Chemical Biology 13, 343 (2017). doi:10.1038/nchembio.2350 Author: Caitlin…

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Imaging: Luciferase matchmaker

Posted on Mar 22nd, 2017 - By Caitlin Deane

Nature Chemical Biology 13, 343 (2017). doi:10.1038/nchembio.2352 Author: Caitlin…

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Versatile modes of cellular regulation via cyclic dinucleotides

Posted on Mar 22nd, 2017 - By Petya Violinova Krasteva

Nature Chemical Biology 13, 350 (2017). doi:10.1038/nchembio.2337 Authors: Petya Violinova Krasteva & Holger…

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Signaling: Spatial regulation of axonal cAMP

Posted on Mar 22nd, 2017 - By Pierre Vincent

Nature Chemical Biology 13, 348 (2017). doi:10.1038/nchembio.2339 Authors: Pierre Vincent & Liliana R Castro In early-stage developing neurons, the cAMP–PKA (protein kinase A) signaling pathway is strongly inhibited. This negative control is later removed, unleashing cAMP–PKA signaling, particularly in distal axonal parts, thus allowing for axonal…

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Protein folding: Illuminating chaperone activity

Posted on Mar 22nd, 2017 - By Danny M Hatters

Nature Chemical Biology 13, 346 (2017). doi:10.1038/nchembio.2332 Author: Danny M Hatters Pharmacological chaperones are small drugs that stabilize a protein's fold and are being developed to treat diseases arising from protein misfolding. A mathematical framework to model their activity in cells enables insight into their mechanism and capacity to rescue protein…

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Simultaneous quantification of protein order and disorder

Posted on Mar 22nd, 2017 - By Pietro Sormanni

Nature Chemical Biology 13, 339 (2017). doi:10.1038/nchembio.2331 Authors: Pietro Sormanni, Damiano Piovesan, Gabriella T Heller, Massimiliano Bonomi, Predrag Kukic, Carlo Camilloni, Monika Fuxreiter, Zsuzsanna Dosztanyi, Rohit V Pappu, M Madan Babu, Sonia Longhi, Peter Tompa, A Keith Dunker, Vladimir N Uversky, Silvio C E Tosatto & Michele Vendruscolo Nuclear magnetic resonance spectroscopy is transforming our views of proteins by revealing how their structures and dynamics…

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A Vibrio cholerae autoinducer–receptor pair that controls biofilm formation

Posted on Mar 20th, 2017 - By Kai Papenfort

Nature Chemical Biology 13, 551 (2017). doi:10.1038/nchembio.2336 Authors: Kai Papenfort, Justin E Silpe, Kelsey R Schramma, Jian-Ping Cong, Mohammad R Seyedsayamdost & Bonnie L…

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The direct role of selenocysteine in [NiFeSe] hydrogenase maturation and catalysis

Posted on Mar 20th, 2017 - By Marta C Marques

Nature Chemical Biology 13, 544 (2017). doi:10.1038/nchembio.2335 Authors: Marta C Marques, Cristina Tapia, Oscar Gutiérrez-Sanz, Ana Raquel Ramos, Kimberly L Keller, Judy D Wall, Antonio L De Lacey, Pedro M Matias & Inês A C…

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Metagenomic discovery of polybrominated diphenyl ether biosynthesis by marine sponges

Posted on Mar 20th, 2017 - By Vinayak Agarwal

Nature Chemical Biology 13, 537 (2017). doi:10.1038/nchembio.2330 Authors: Vinayak Agarwal, Jessica M Blanton, Sheila Podell, Arnaud Taton, Michelle A Schorn, Julia Busch, Zhenjian Lin, Eric W Schmidt, Paul R Jensen, Valerie J Paul, Jason S Biggs, James W Golden, Eric E Allen & Bradley S…

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Decoding cyclase-dependent assembly of hapalindole and fischerindole alkaloids

Posted on Mar 13th, 2017 - By Shasha Li

Nature Chemical Biology 13, 467 (2017). doi:10.1038/nchembio.2327 Authors: Shasha Li, Andrew N Lowell, Sean A Newmister, Fengan Yu, Robert M Williams & David H Sherman The formation of C–C bonds in an enantioselective fashion to create complex polycyclic scaffolds in the hapalindole- and fischerindole- type alkaloids from Stigonematales cyanobacteria represents a compelling and urgent challenge in adapting microbial biosynthesis as a catalytic platform in drug…

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Structural basis of PROTAC cooperative recognition for selective protein degradation

Posted on Mar 13th, 2017 - By Morgan S Gadd

Nature Chemical Biology 13, 514 (2017). doi:10.1038/nchembio.2329 Authors: Morgan S Gadd, Andrea Testa, Xavier Lucas, Kwok-Ho Chan, Wenzhang Chen, Douglas J Lamont, Michael Zengerle & Alessio…

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The Rrp4–exosome complex recruits and channels substrate RNA by a unique mechanism

Posted on Mar 13th, 2017 - By Milos A Cvetkovic

Nature Chemical Biology 13, 522 (2017). doi:10.1038/nchembio.2328 Authors: Milos A Cvetkovic, Jan Philip Wurm, Maxime J Audin, Stefan Schütz & Remco…

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In silico design of novel probes for the atypical opioid receptor MRGPRX2

Posted on Mar 13th, 2017 - By Katherine Lansu

Nature Chemical Biology 13, 529 (2017). doi:10.1038/nchembio.2334 Authors: Katherine Lansu, Joel Karpiak, Jing Liu, Xi-Ping Huang, John D McCorvy, Wesley K Kroeze, Tao Che, Hiroshi Nagase, Frank I Carroll, Jian Jin, Brian K Shoichet & Bryan L…

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L-2-Hydroxyglutarate production arises from noncanonical enzyme function at acidic pH

Posted on Mar 6th, 2017 - By Andrew M Intlekofer

Nature Chemical Biology 13, 494 (2017). doi:10.1038/nchembio.2307 Authors: Andrew M Intlekofer, Bo Wang, Hui Liu, Hardik Shah, Carlos Carmona-Fontaine, Ariën S Rustenburg, Salah Salah, M R Gunner, John D Chodera, Justin R Cross & Craig B…

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The structure of a nucleolytic ribozyme that employs a catalytic metal ion

Posted on Mar 6th, 2017 - By Yijin Liu

Nature Chemical Biology 13, 508 (2017). doi:10.1038/nchembio.2333 Authors: Yijin Liu, Timothy J Wilson & David M J…

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Total RNA-seq to identify pharmacological effects on specific stages of mRNA synthesis

Posted on Mar 6th, 2017 - By Sarah A Boswell

Nature Chemical Biology 13, 501 (2017). doi:10.1038/nchembio.2317 Authors: Sarah A Boswell, Andrew Snavely, Heather M Landry, L Stirling Churchman, Jesse M Gray & Michael…

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Capzimin is a potent and specific inhibitor of proteasome isopeptidase Rpn11

Posted on Feb 28th, 2017 - By Jing Li

Nature Chemical Biology 13, 486 (2017). doi:10.1038/nchembio.2326 Authors: Jing Li, Tanya Yakushi, Francesco Parlati, Andrew L Mackinnon, Christian Perez, Yuyong Ma, Kyle P Carter, Sharon Colayco, Gavin Magnuson, Brock Brown, Kevin Nguyen, Stefan Vasile, Eigo Suyama, Layton H Smith, Eduard Sergienko, Anthony B Pinkerton, Thomas D Y Chung, Amy E Palmer, Ian Pass, Sonja Hess, Seth M Cohen & Raymond J…

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A fully automated flow-based approach for accelerated peptide synthesis

Posted on Feb 28th, 2017 - By Alexander J Mijalis

Nature Chemical Biology 13, 464 (2017). doi:10.1038/nchembio.2318 Authors: Alexander J Mijalis, Dale A Thomas, Mark D Simon, Andrea Adamo, Ryan Beaumont, Klavs F Jensen & Bradley L Pentelute Here we report a fully automated, flow-based approach to solid-phase polypeptide synthesis, with amide bond formation in 7 seconds and total synthesis times of 40 seconds per amino acid residue. Crude peptide purities and isolated yields were comparable to those for standard-batch…

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A new genome-mining tool redefines the lasso peptide biosynthetic landscape

Posted on Feb 28th, 2017 - By Jonathan I Tietz

Nature Chemical Biology 13, 470 (2017). doi:10.1038/nchembio.2319 Authors: Jonathan I Tietz, Christopher J Schwalen, Parth S Patel, Tucker Maxson, Patricia M Blair, Hua-Chia Tai, Uzma I Zakai & Douglas A…

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The Arabidopsis O-fucosyltransferase SPINDLY activates nuclear growth repressor DELLA

Posted on Feb 28th, 2017 - By Rodolfo Zentella

Nature Chemical Biology 13, 479 (2017). doi:10.1038/nchembio.2320 Authors: Rodolfo Zentella, Ning Sui, Benjamin Barnhill, Wen-Ping Hsieh, Jianhong Hu, Jeffrey Shabanowitz, Michael Boyce, Neil E Olszewski, Pei Zhou, Donald F Hunt & Tai-ping…

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Ligand-promoted protein folding by biased kinetic partitioning

Posted on Feb 20th, 2017 - By Karan S Hingorani

Nature Chemical Biology 13, 369 (2017). doi:10.1038/nchembio.2303 Authors: Karan S Hingorani, Matthew C Metcalf, Derrick T Deming, Scott C Garman, Evan T Powers & Lila M Gierasch Protein folding in cells occurs in the presence of high concentrations of endogenous binding partners, and exogenous binding partners have been exploited as pharmacological chaperones. A combined mathematical modeling and experimental approach shows that a ligand improves the folding of a…

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Expanding the product portfolio of fungal type I fatty acid synthases

Posted on Feb 20th, 2017 - By Zhiwei Zhu

Nature Chemical Biology 13, 360 (2017). doi:10.1038/nchembio.2301 Authors: Zhiwei Zhu, Yongjin J Zhou, Anastasia Krivoruchko, Martin Grininger, Zongbao K Zhao & Jens Nielsen Fungal type I fatty acid synthases (FASs) are mega-enzymes with two separated, identical compartments, in which the acyl carrier protein (ACP) domains shuttle substrates to catalytically active sites embedded in the chamber wall. We devised synthetic FASs by integrating heterologous enzymes into the…

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Engineering fatty acid synthases for directed polyketide production

Posted on Feb 20th, 2017 - By Jan Gajewski

Nature Chemical Biology 13, 363 (2017). doi:10.1038/nchembio.2314 Authors: Jan Gajewski, Floris Buelens, Sascha Serdjukow, Melanie Janßen, Niña Cortina, Helmut Grubmüller & Martin Grininger In this study, we engineered fatty acid synthases (FAS) for the biosynthesis of short-chain fatty acids and polyketides, guided by a combined in vitro and in silico approach. Along with exploring the synthetic capability of FAS, we aim to build a foundation for efficient…

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Accelerating the semisynthesis of alkaloid-based drugs through metabolic engineering

Posted on Feb 15th, 2017 - By Amy M Ehrenworth

Nature Chemical Biology 13, 249 (2017). doi:10.1038/nchembio.2308 Authors: Amy M Ehrenworth & Pamela…

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mRNA localization: If you have to ASH

Posted on Feb 15th, 2017 - By Joshua M. Finkelstein

Nature Chemical Biology 13, 243 (2017). doi:10.1038/nchembio.2323 Author: Joshua M.…

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Synthetic biology: Synthetic gene networks that smell

Posted on Feb 15th, 2017 - By Fahim Farzadfard

Nature Chemical Biology 13, 245 (2017). doi:10.1038/nchembio.2315 Authors: Fahim Farzadfard & Timothy K Lu Bioengineers have endowed a consortium of human cells with an artificial sense of smell, enabling the cells to detect, quantify, and remember the presence of gaseous volatile compounds in their…

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Enzyme mechanisms: Fickle about fluorine

Posted on Feb 15th, 2017 - By Caitlin Deane

Nature Chemical Biology 13, 243 (2017). doi:10.1038/nchembio.2324 Author: Caitlin…

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Plant infection: A decoy tactic

Posted on Feb 15th, 2017 - By Grant Miura

Nature Chemical Biology 13, 243 (2017). doi:10.1038/nchembio.2322 Author: Grant…

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Metabolism: A-way with biofilms

Posted on Feb 15th, 2017 - By Mirella Bucci

Nature Chemical Biology 13, 243 (2017). doi:10.1038/nchembio.2325 Author: Mirella…

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G-Protein-coupled receptors: Decoding mixed signals

Posted on Feb 15th, 2017 - By Thomas J Gardella

Nature Chemical Biology 13, 247 (2017). doi:10.1038/nchembio.2316 Author: Thomas J Gardella A new mechanism of functional crosstalk between two distinct G-protein-coupled receptors (GPCRs)—the parathyroid hormone receptor (PTHR) and β2-adrenergic receptor (β2 Ar)—that occurs at the level of G protein βγ subunits and a specific adenylyl cyclase isoform is identified. This crosstalk augments cAMP signaling by the PTHR from endosomes, and thus…

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Selective in vivo metabolic cell-labeling-mediated cancer targeting

Posted on Feb 13th, 2017 - By Hua Wang

Nature Chemical Biology 13, 415 (2017). doi:10.1038/nchembio.2297 Authors: Hua Wang, Ruibo Wang, Kaimin Cai, Hua He, Yang Liu, Jonathan Yen, Zhiyu Wang, Ming Xu, Yiwen Sun, Xin Zhou, Qian Yin, Li Tang, Iwona T Dobrucki, Lawrence W Dobrucki, Eric J Chaney, Stephen A Boppart, Timothy M Fan, Stéphane Lezmi, Xuesi Chen, Lichen Yin & Jianjun…

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AKAP-mediated feedback control of cAMP gradients in developing hippocampal neurons

Posted on Feb 13th, 2017 - By Kirill Gorshkov

Nature Chemical Biology 13, 425 (2017). doi:10.1038/nchembio.2298 Authors: Kirill Gorshkov, Sohum Mehta, Santosh Ramamurthy, Gabriele V Ronnett, Feng-Quan Zhou & Jin…

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An orthogonalized platform for genetic code expansion in both bacteria and eukaryotes

Posted on Feb 13th, 2017 - By James S Italia

Nature Chemical Biology 13, 446 (2017). doi:10.1038/nchembio.2312 Authors: James S Italia, Partha Sarathi Addy, Chester J J Wrobel, Lisa A Crawford, Marc J Lajoie, Yunan Zheng & Abhishek…

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Evolution of a split RNA polymerase as a versatile biosensor platform

Posted on Feb 13th, 2017 - By Jinyue Pu

Nature Chemical Biology 13, 432 (2017). doi:10.1038/nchembio.2299 Authors: Jinyue Pu, Julia Zinkus-Boltz & Bryan C…

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The GlcN6P cofactor plays multiple catalytic roles in the glmS ribozyme

Posted on Feb 13th, 2017 - By Jamie L Bingaman

Nature Chemical Biology 13, 439 (2017). doi:10.1038/nchembio.2300 Authors: Jamie L Bingaman, Sixue Zhang, David R Stevens, Neela H Yennawar, Sharon Hammes-Schiffer & Philip C…

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In vitro reconstitution demonstrates the cell wall ligase activity of LCP proteins

Posted on Feb 6th, 2017 - By Kaitlin Schaefer

Nature Chemical Biology 13, 396 (2017). doi:10.1038/nchembio.2302 Authors: Kaitlin Schaefer, Leigh M Matano, Yuan Qiao, Daniel Kahne & Suzanne…

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A water-mediated allosteric network governs activation of Aurora kinase A

Posted on Feb 6th, 2017 - By Soreen Cyphers

Nature Chemical Biology 13, 402 (2017). doi:10.1038/nchembio.2296 Authors: Soreen Cyphers, Emily F Ruff, Julie M Behr, John D Chodera & Nicholas M…

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A tight tunable range for Ni(II) sensing and buffering in cells

Posted on Feb 6th, 2017 - By Andrew W Foster

Nature Chemical Biology 13, 409 (2017). doi:10.1038/nchembio.2310 Authors: Andrew W Foster, Rafael Pernil, Carl J Patterson, Andrew J P Scott, Lars-Olof Pålsson, Robert Pal, Ian Cummins, Peter T Chivers, Ehmke Pohl & Nigel J…

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Structure and specificity of a permissive bacterial C-prenyltransferase

Posted on Feb 6th, 2017 - By Sherif I Elshahawi

Nature Chemical Biology 13, 366 (2017). doi:10.1038/nchembio.2285 Authors: Sherif I Elshahawi, Hongnan Cao, Khaled A Shaaban, Larissa V Ponomareva, Thangaiah Subramanian, Mark L Farman, H Peter Spielmann, George N Phillips, Jon S Thorson & Shanteri Singh This study highlights the biochemical and structural characterization of the L-tryptophan C6 C-prenyltransferase (C-PT) PriB from Streptomyces sp. RM-5-8. PriB was found to be uniquely permissive to a diverse array of prenyl…

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Chromatin biology: Breaking into the PRC2 cage

Posted on Jan 30th, 2017 - By Daniel Holoch

Nature Chemical Biology 13, 345 (2017). doi:10.1038/nchembio.2313 Authors: Daniel Holoch & Raphaël Margueron New small-molecule inhibitors of the histone methyltransferase PRC2 interfere with the allosteric activation of enzymatic activity. These compounds are effective against PRC2-dependent tumors that are resistant to catalytic inhibitors and provide important new tools for altering chromatin…

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HTS-compatible FRET-based conformational sensors clarify membrane receptor activation

Posted on Jan 30th, 2017 - By Pauline Scholler

Nature Chemical Biology 13, 372 (2017). doi:10.1038/nchembio.2286 Authors: Pauline Scholler, David Moreno-Delgado, Nathalie Lecat-Guillet, Etienne Doumazane, Carine Monnier, Fabienne Charrier-Savournin, Ludovic Fabre, Cédric Chouvet, Stéphanie Soldevila, Laurent Lamarque, Geoffrey Donsimoni, Thomas Roux, Jurriaan M Zwier, Eric Trinquet, Philippe Rondard & Jean-Philippe…

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The EED protein–protein interaction inhibitor A-395 inactivates the PRC2 complex

Posted on Jan 30th, 2017 - By Yupeng He

Nature Chemical Biology 13, 389 (2017). doi:10.1038/nchembio.2306 Authors: Yupeng He, Sujatha Selvaraju, Michael L Curtin, Clarissa G Jakob, Haizhong Zhu, Kenneth M Comess, Bailin Shaw, Juliana The, Evelyne Lima-Fernandes, Magdalena M Szewczyk, Dong Cheng, Kelly L Klinge, Huan-Qiu Li, Marina Pliushchev, Mikkel A Algire, David Maag, Jun Guo, Justin Dietrich, Sanjay C Panchal, Andrew M Petros, Ramzi F Sweis, Maricel Torrent, Lance J Bigelow, Guillermo Senisterra, Fengling Li,…

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An allosteric PRC2 inhibitor targeting the H3K27me3 binding pocket of EED

Posted on Jan 30th, 2017 - By Wei Qi

Nature Chemical Biology 13, 381 (2017). doi:10.1038/nchembio.2304 Authors: Wei Qi, Kehao Zhao, Justin Gu, Ying Huang, Youzhen Wang, Hailong Zhang, Man Zhang, Jeff Zhang, Zhengtian Yu, Ling Li, Lin Teng, Shannon Chuai, Chao Zhang, Mengxi Zhao, HoMan Chan, Zijun Chen, Douglas Fang, Qi Fei, Leying Feng, Lijian Feng, Yuan Gao, Hui Ge, Xinjian Ge, Guobin Li, Andreas Lingel, Ying Lin, Yueqin Liu, Fangjun Luo, Minlong Shi, Long Wang, Zhaofu Wang, Yanyan Yu, Jue Zeng, Chenhui Zeng, Lijun…

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Redox regulation: Taking AKTion on HNEs

Posted on Jan 23rd, 2017 - By Eranthie Weerapana

Nature Chemical Biology 13, 244 (2017). doi:10.1038/nchembio.2311 Author: Eranthie Weerapana Differential redox regulation of kinase isoforms serves to provide intricate control of cellular signaling events. In a new study, a single isoform of Akt, Akt3, is shown to be preferentially modified by lipid-derived electrophiles to modulate downstream signaling events in mammalian cells and…

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The SUV4-20 inhibitor A-196 verifies a role for epigenetics in genomic integrity

Posted on Jan 23rd, 2017 - By Kenneth D Bromberg

Nature Chemical Biology 13, 317 (2017). doi:10.1038/nchembio.2282 Authors: Kenneth D Bromberg, Taylor R H Mitchell, Anup K Upadhyay, Clarissa G Jakob, Manisha A Jhala, Kenneth M Comess, Loren M Lasko, Conglei Li, Creighton T Tuzon, Yujia Dai, Fengling Li, Mohammad S Eram, Alexander Nuber, Niru B Soni, Vlasios Manaves, Mikkel A Algire, Ramzi F Sweis, Maricel Torrent, Gunnar Schotta, Chaohong Sun, Michael R Michaelides, Alex R Shoemaker, Cheryl H Arrowsmith, Peter J Brown,…

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Akt3 is a privileged first responder in isozyme-specific electrophile response

Posted on Jan 23rd, 2017 - By Marcus J C Long

Nature Chemical Biology 13, 333 (2017). doi:10.1038/nchembio.2284 Authors: Marcus J C Long, Saba Parvez, Yi Zhao, Sanjna L Surya, Yiran Wang, Sheng Zhang & Yimon…

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Enzyme-catalyzed cationic epoxide rearrangements in quinolone alkaloid biosynthesis

Posted on Jan 23rd, 2017 - By Yi Zou

Nature Chemical Biology 13, 325 (2017). doi:10.1038/nchembio.2283 Authors: Yi Zou, Marc Garcia-Borràs, Mancheng C Tang, Yuichiro Hirayama, Dehai H Li, Li Li, Kenji Watanabe, K N Houk & Yi…

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Host-Pathogen interactions: Nucleotide circles of life and death

Posted on Jan 19th, 2017 - By Lingyin Li

Nature Chemical Biology 13, 130 (2017). doi:10.1038/nchembio.2289 Author: Lingyin Li A phosphodiesterase, CdnP, from Mycobacterium tuberculosis (M. tb.) helps the pathogen evade immune detection by degrading the second messenger cyclic di-AMP that alerts the host to its presence. Genetic knockout of CdnP dampens the virulence of the pathogen, suggesting that CdnP inhibitors are potential anti–M. tb.…

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Pancreatic development: Changing identity

Posted on Jan 19th, 2017 - By Grant Miura

Nature Chemical Biology 13, 129 (2017). doi:10.1038/nchembio.2293 Author: Grant…

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Target engagement: Shining a light

Posted on Jan 19th, 2017 - By Kilian V M Huber

Nature Chemical Biology 13, 133 (2017). doi:10.1038/nchembio.2295 Author: Kilian V M Huber The ability to measure the binding of a compound to its intended target in live cells or tissue is a critical parameter for drug discovery. A new method using polarized light microscopy adds to the current toolbox by enabling monitoring of target engagement in vitro and in vivo at single-cell…

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RNA structure: Untying Zika’s knots

Posted on Jan 19th, 2017 - By Terry L. Sheppard

Nature Chemical Biology 13, 129 (2017). doi:10.1038/nchembio.2294 Author: Terry L.…

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The pharmacological regulation of cellular mitophagy

Posted on Jan 19th, 2017 - By Nikolaos D Georgakopoulos

Nature Chemical Biology 13, 136 (2017). doi:10.1038/nchembio.2287 Authors: Nikolaos D Georgakopoulos, Geoff Wells & Michelangelo…

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Synthetic biology: Sensing with modular receptors

Posted on Jan 19th, 2017 - By Matthew Brenner

Nature Chemical Biology 13, 131 (2017). doi:10.1038/nchembio.2290 Authors: Matthew Brenner, Jang Hwan Cho & Wilson W Wong Sensing and responding to diverse extracellular signals is a crucial aspect of cellular decision-making that is currently lacking in the synthetic biology toolkit. The development of modular receptor platforms allows for the rewiring of cellular input–output…

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Susan Lindquist (1949–2016)

Posted on Jan 19th, 2017 - By Tricia R Serio

Nature Chemical Biology 13, 127 (2017). doi:10.1038/nchembio.2305 Author: Tricia R Serio Susan Lindquist passed away on 27 October 2016, far too early for those who marveled at her brilliance through her many contributions to science, for all who knew her directly, and especially for her former…

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RNA modification: Reading Sex-lethal

Posted on Jan 19th, 2017 - By Grant Miura

Nature Chemical Biology 13, 129 (2017). doi:10.1038/nchembio.2291 Author: Grant…

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Nitrogenase: A fuel-producing microbe

Posted on Jan 19th, 2017 - By Holger Dobbek

Nature Chemical Biology 13, 134 (2017). doi:10.1038/nchembio.2288 Author: Holger Dobbek Nitrogenase has the canonical ability to reduce N2 to NH3, but under certain conditions, either in vitro or in vivo, it has the additional capability to convert CO2 to CO and CO to light…

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Biosynthesis: Terrifically tailored peptides

Posted on Jan 19th, 2017 - By Caitlin Deane

Nature Chemical Biology 13, 129 (2017). doi:10.1038/nchembio.2292 Author: Caitlin…

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Designed cell consortia as fragrance-programmable analog-to-digital converters

Posted on Jan 16th, 2017 - By Marius Müller

Nature Chemical Biology 13, 309 (2017). doi:10.1038/nchembio.2281 Authors: Marius Müller, Simon Ausländer, Andrea Spinnler, David Ausländer, Julian Sikorski, Marc Folcher & Martin…

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Recurrent RNA motifs as scaffolds for genetically encodable small-molecule biosensors

Posted on Jan 16th, 2017 - By Ely B Porter

Nature Chemical Biology 13, 295 (2017). doi:10.1038/nchembio.2278 Authors: Ely B Porter, Jacob T Polaski, Makenna M Morck & Robert T…

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Chemical proteomics reveals ADP-ribosylation of small GTPases during oxidative stress

Posted on Jan 16th, 2017 - By Nathan P Westcott

Nature Chemical Biology 13, 302 (2017). doi:10.1038/nchembio.2280 Authors: Nathan P Westcott, Joseph P Fernandez, Henrik Molina & Howard C…

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