Protein engineering: Expanding splicing versatility

Posted on Sep 19th, 2017 - By Karin Kuehnel

Nature Chemical Biology 13, 1057 (2017). doi:10.1038/nchembio.2485 Author: Karin…

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Bioorthogonal chemistry: Click on, click off

Posted on Sep 19th, 2017 - By Caitlin Deane

Nature Chemical Biology 13, 1057 (2017). doi:10.1038/nchembio.2482 Author: Caitlin…

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Developmental signaling: Peaking early

Posted on Sep 19th, 2017 - By Grant Miura

Nature Chemical Biology 13, 1057 (2017). doi:10.1038/nchembio.2484 Author: Grant…

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Target class drug discovery

Posted on Sep 19th, 2017 - By Kimberly D Barnash

Nature Chemical Biology 13, 1053 (2017). doi:10.1038/nchembio.2473 Authors: Kimberly D Barnash, Lindsey I James & Stephen V Frye Selection of molecular targets based on disease understanding is a dominant paradigm in drug discovery. We argue that a focus on classes of targets with central roles in biology provides a complimentary approach that has higher quality outcomes in early discovery…

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Genome mining: Digging the tunnel for chemical space

Posted on Sep 19th, 2017 - By Ha An Nguyen

Nature Chemical Biology 13, 1061 (2017). doi:10.1038/nchembio.2480 Authors: Ha An Nguyen & Christine M Dunham Genome mining reveals a new ribosomally synthesized and post-translationally modified peptide (RiPP) from Klebsiella pneumoniae. This new antibiotic inhibits bacterial ribosomes by obstructing the peptide exit tunnel, and its modular nature presents a unique opportunity for future engineering of antibacterial…

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Biosynthesis: SAM cycles up for colibactin

Posted on Sep 19th, 2017 - By Steven G Van Lanen

Nature Chemical Biology 13, 1059 (2017). doi:10.1038/nchembio.2479 Author: Steven G Van Lanen The introduction of a cyclopropyl group is critical for imparting colibactin with the ability to cleave DNA. Nonribosomal peptide synthetase ClbH and polyketide synthase ClbI are now shown to work in concert to convert S-adenosylmethionine into this cyclopropyl…

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Erratum: A Vibrio cholerae autoinducer–receptor pair that controls biofilm formation

Posted on Sep 19th, 2017 - By Kai Papenfort

Nature Chemical Biology 13, 1137 (2017). doi:10.1038/nchembio1017-1137a Author: Kai Papenfort, Justin E Silpe, Kelsey R Schramma, Jian-Ping Cong, Mohammad R Seyedsayamdost & Bonnie L…

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Corrigendum: Parallel evolution of non-homologous isofunctional enzymes in methionine biosynthesis

Posted on Sep 19th, 2017 - By Karine Bastard

Nature Chemical Biology 13, 1137 (2017). doi:10.1038/nchembio1017-1137c Author: Karine Bastard, Alain Perret, Aline Mariage, Thomas Bessonnet, Agnès Pinet-Turpault, Jean-Louis Petit, Ekaterina Darii, Pascal Bazire, Carine Vergne-Vaxelaire, Clémence Brewee, Adrien Debard, Virginie Pellouin, Marielle Besnard-Gonnet, François Artiguenave, Claudine Médigue, David Vallenet, Antoine Danchin, Anne Zaparucha, Jean Weissenbach, Marcel Salanoubat & Véronique de…

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Erratum: A Vibrio cholerae autoinducer–receptor pair that controls biofilm formation

Posted on Sep 19th, 2017 - By Kai Papenfort

Nature Chemical Biology 13, 1137 (2017). doi:10.1038/nchembio1017-1137b Author: Kai Papenfort, Justin E Silpe, Kelsey R Schramma, Jian-Ping Cong, Mohammad R Seyedsayamdost & Bonnie L…

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Chemical methods: DREADDs see no CNO

Posted on Sep 19th, 2017 - By Mirella Bucci

Nature Chemical Biology 13, 1057 (2017). doi:10.1038/nchembio.2483 Author: Mirella…

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Klebsazolicin inhibits 70S ribosome by obstructing the peptide exit tunnel

Posted on Aug 28th, 2017 - By Mikhail Metelev

Nature Chemical Biology 13, 1129 (2017). doi:10.1038/nchembio.2462 Authors: Mikhail Metelev, Ilya A Osterman, Dmitry Ghilarov, Nelli F Khabibullina, Alexander Yakimov, Konstantin Shabalin, Irina Utkina, Dmitry Y Travin, Ekaterina S Komarova, Marina Serebryakova, Tatyana Artamonova, Mikhail Khodorkovskii, Andrey L Konevega, Petr V Sergiev, Konstantin Severinov & Yury S…

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Oxidative cleavage of polysaccharides by monocopper enzymes depends on H2O2

Posted on Aug 28th, 2017 - By Bastien Bissaro

Nature Chemical Biology 13, 1123 (2017). doi:10.1038/nchembio.2470 Authors: Bastien Bissaro, Åsmund K Røhr, Gerdt Müller, Piotr Chylenski, Morten Skaugen, Zarah Forsberg, Svein J Horn, Gustav Vaaje-Kolstad & Vincent G H…

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Metals induce transient folding and activation of the twister ribozyme

Posted on Aug 21st, 2017 - By Subrata Panja

Nature Chemical Biology 13, 1109 (2017). doi:10.1038/nchembio.2459 Authors: Subrata Panja, Boyang Hua, Diego Zegarra, Taekjip Ha & Sarah A…

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Crystal structures of trimeric HIV envelope with entry inhibitors BMS-378806 and BMS-626529

Posted on Aug 21st, 2017 - By Marie Pancera

Nature Chemical Biology 13, 1115 (2017). doi:10.1038/nchembio.2460 Authors: Marie Pancera, Yen-Ting Lai, Tatsiana Bylund, Aliaksandr Druz, Sandeep Narpala, Sijy O'Dell, Arne Schön, Robert T Bailer, Gwo-Yu Chuang, Hui Geng, Mark K Louder, Reda Rawi, Djade I Soumana, Andrés Finzi, Alon Herschhorn, Navid Madani, Joseph Sodroski, Ernesto Freire, David R Langley, John R Mascola, Adrian B McDermott & Peter D…

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Bacterial immunity: A virtuous CRISPR cycle

Posted on Aug 18th, 2017 - By Terry L. Sheppard

Nature Chemical Biology 13, 923 (2017). doi:10.1038/nchembio.2468 Author: Terry L.…

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Drug development: Locking down metabolism

Posted on Aug 18th, 2017 - By William R Bishai

Nature Chemical Biology 13, 925 (2017). doi:10.1038/nchembio.2452 Author: William R Bishai An allosteric inhibitor of Mycobacterium tuberculosis tryptophan synthase—an enzyme that is nonessential for in vitro growth—has potent antimicrobial activity, revealing a potentially expanded target list for antimicrobials and greater chemical space for new…

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A kinetic view of GPCR allostery and biased agonism

Posted on Aug 18th, 2017 - By J Robert Lane

Nature Chemical Biology 13, 929 (2017). doi:10.1038/nchembio.2431 Authors: J Robert Lane, Lauren T May, Robert G Parton, Patrick M Sexton & Arthur…

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Ubiquitin-proteasome system: Rescuing EBV latency

Posted on Aug 18th, 2017 - By Mirella Bucci

Nature Chemical Biology 13, 923 (2017). doi:10.1038/nchembio.2466 Author: Mirella…

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Protein engineering: Redirecting membrane machinery

Posted on Aug 18th, 2017 - By Kalistyn Burley

Nature Chemical Biology 13, 927 (2017). doi:10.1038/nchembio.2451 Authors: Kalistyn Burley & Celia W Goulding The ability to solubilize membrane proteins while retaining their native function is a persistent challenge. Re-engineering of the membrane protein DsbB into a soluble cytoplasmic version maintained its activity and enabled recompartmentalization of the periplasmic DsbAB disulfide bond–forming…

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Extracellular vesicles: Taking metabolism on the road

Posted on Aug 18th, 2017 - By Lucas B Sullivan

Nature Chemical Biology 13, 924 (2017). doi:10.1038/nchembio.2455 Author: Lucas B Sullivan Extracellular vesicles (EVs) are a class of secreted membrane particles capable of transferring biological molecules between cells. Metabolomics measurements indicate that isolated EVs also have autonomous metabolic enzyme activities, including the unexpected identification of endogenous human asparaginase…

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Protein design: I like to fold it, fold it

Posted on Aug 18th, 2017 - By Caitlin Deane

Nature Chemical Biology 13, 923 (2017). doi:10.1038/nchembio.2467 Author: Caitlin…

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Neurobiology: Lighting up neurons

Posted on Aug 18th, 2017 - By Grant Miura

Nature Chemical Biology 13, 923 (2017). doi:10.1038/nchembio.2469 Author: Grant…

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Small-molecule studies identify CDK8 as a regulator of IL-10 in myeloid cells

Posted on Aug 14th, 2017 - By Liv Johannessen

Nature Chemical Biology 13, 1102 (2017). doi:10.1038/nchembio.2458 Authors: Liv Johannessen, Thomas B Sundberg, Daniel J O'Connell, Raivo Kolde, James Berstler, Katelyn J Billings, Bernard Khor, Brinton Seashore-Ludlow, Anne Fassl, Caitlin N Russell, Isabel J Latorre, Baishan Jiang, Daniel B Graham, Jose R Perez, Piotr Sicinski, Andrew J Phillips, Stuart L Schreiber, Nathanael S Gray, Alykhan F Shamji & Ramnik J…

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Optogenetic control of kinetochore function

Posted on Aug 14th, 2017 - By Huaiying Zhang

Nature Chemical Biology 13, 1096 (2017). doi:10.1038/nchembio.2456 Authors: Huaiying Zhang, Chanat Aonbangkhen, Ekaterina V Tarasovetc, Edward R Ballister, David M Chenoweth & Michael A…

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Kinetochore regulation: Let there be light

Posted on Aug 14th, 2017 - By Ana C Figueiredo

Nature Chemical Biology 13, 1058 (2017). doi:10.1038/nchembio.2464 Authors: Ana C Figueiredo & Helder Maiato Kinetochores form the critical interface with spindle microtubules that accounts for chromosome movement and segregation fidelity during mitosis. Spatial and temporal control of motor protein and checkpoint signaling at kinetochores is now possible with a new set of optogenetic…

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Colibactin assembly line enzymes use S-adenosylmethionine to build a cyclopropane ring

Posted on Aug 7th, 2017 - By Li Zha

Nature Chemical Biology 13, 1063 (2017). doi:10.1038/nchembio.2448 Authors: Li Zha, Yindi Jiang, Matthew T Henke, Matthew R Wilson, Jennifer X Wang, Neil L Kelleher & Emily P Balskus Despite containing an α-amino acid, the versatile cofactor S-adenosylmethionine (SAM) is not a known building block for nonribosomal peptide synthetase (NRPS) assembly lines. Here we report an unusual NRPS module from colibactin biosynthesis that uses SAM for amide bond formation and…

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Bisphosphoglycerate mutase controls serine pathway flux via 3-phosphoglycerate

Posted on Aug 7th, 2017 - By Rob C Oslund

Nature Chemical Biology 13, 1081 (2017). doi:10.1038/nchembio.2453 Authors: Rob C Oslund, Xiaoyang Su, Michael Haugbro, Jung-Min Kee, Mark Esposito, Yael David, Boyuan Wang, Eva Ge, David H Perlman, Yibin Kang, Tom W Muir & Joshua D…

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A genetically encoded tool for manipulation of NADP+/NADPH in living cells

Posted on Aug 7th, 2017 - By Valentin Cracan

Nature Chemical Biology 13, 1088 (2017). doi:10.1038/nchembio.2454 Authors: Valentin Cracan, Denis V Titov, Hongying Shen, Zenon Grabarek & Vamsi K…

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Molecular basis for the unusual ring reconstruction in fungal meroterpenoid biogenesis

Posted on Jul 31st, 2017 - By Takahiro Mori

Nature Chemical Biology 13, 1066 (2017). doi:10.1038/nchembio.2443 Authors: Takahiro Mori, Taiki Iwabuchi, Shotaro Hoshino, Hang Wang, Yudai Matsuda & Ikuro…

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Modulating the DNA polymerase β reaction equilibrium to dissect the reverse reaction

Posted on Jul 31st, 2017 - By David D Shock

Nature Chemical Biology 13, 1074 (2017). doi:10.1038/nchembio.2450 Authors: David D Shock, Bret D Freudenthal, William A Beard & Samuel H…

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Copper import in Escherichia coli by the yersiniabactin metallophore system

Posted on Jul 24th, 2017 - By Eun-Ik Koh

Nature Chemical Biology 13, 1016 (2017). doi:10.1038/nchembio.2441 Authors: Eun-Ik Koh, Anne E Robinson, Nilantha Bandara, Buck E Rogers & Jeffrey P…

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FRET monitoring of a nonribosomal peptide synthetase

Posted on Jul 24th, 2017 - By Jonas Alfermann

Nature Chemical Biology 13, 1009 (2017). doi:10.1038/nchembio.2435 Authors: Jonas Alfermann, Xun Sun, Florian Mayerthaler, Thomas E Morrell, Eva Dehling, Gerrit Volkmann, Tamiki Komatsuzaki, Haw Yang & Henning D…

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Toxicity and repair of DNA adducts produced by the natural product yatakemycin

Posted on Jul 24th, 2017 - By Elwood A Mullins

Nature Chemical Biology 13, 1002 (2017). doi:10.1038/nchembio.2439 Authors: Elwood A Mullins, Rongxin Shi & Brandt F…

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Peptidomimetic inhibitors of APC–Asef interaction block colorectal cancer migration

Posted on Jul 24th, 2017 - By Haiming Jiang

Nature Chemical Biology 13, 994 (2017). doi:10.1038/nchembio.2442 Authors: Haiming Jiang, Rong Deng, Xiuyan Yang, Jialin Shang, Shaoyong Lu, Yanlong Zhao, Kun Song, Xinyi Liu, Qiufen Zhang, Yu Chen, Y Eugene Chinn, Geng Wu, Jian Li, Guoqiang Chen, Jianxiu Yu & Jian…

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Functional annotation of chemical libraries across diverse biological processes

Posted on Jul 24th, 2017 - By Jeff S Piotrowski

Nature Chemical Biology 13, 982 (2017). doi:10.1038/nchembio.2436 Authors: Jeff S Piotrowski, Sheena C Li, Raamesh Deshpande, Scott W Simpkins, Justin Nelson, Yoko Yashiroda, Jacqueline M Barber, Hamid Safizadeh, Erin Wilson, Hiroki Okada, Abraham A Gebre, Karen Kubo, Nikko P Torres, Marissa A LeBlanc, Kerry Andrusiak, Reika Okamoto, Mami Yoshimura, Eva DeRango-Adem, Jolanda van Leeuwen, Katsuhiko Shirahige, Anastasia Baryshnikova, Grant W Brown, Hiroyuki Hirano, Michael…

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Structural biology: Full monty of family B GPCRs

Posted on Jul 18th, 2017 - By Thue W Schwartz

Nature Chemical Biology 13, 819 (2017). doi:10.1038/nchembio.2438 Authors: Thue W Schwartz & Thomas M Frimurer New structures of the glucagon-like peptide-1 (GLP-1) and glucagon receptors in complex with peptide and nonpeptide ligands provide a comprehensive, detailed picture of the molecular mechanisms of action of family B GPCRs. This opens the door for true structure-based drug discovery aimed at both novel orthosteric and allosteric subsites of the…

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Hints of hidden chemistry

Nature Chemical Biology 13, 815 (2017). doi:10.1038/nchembio.2449 Biosynthetic pathways harbor diverse enzyme functions, and identifying those that catalyze unusual or synthetically challenging transformations offers new routes for biocatalytic…

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Biosynthesis: Methylating mushrooms

Posted on Jul 18th, 2017 - By Albert A Bowers

Nature Chemical Biology 13, 821 (2017). doi:10.1038/nchembio.2437 Author: Albert A Bowers The genome of the poisonous mushroom Omphalotus olearius provides a potent new biocatalytic strategy for installing backbone N-methyl amides on ribosomally synthesized peptides. This discovery could yield new biotechnologies for drug development from peptide…

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Neurodegenerative disease: Not stuck on repeat

Posted on Jul 18th, 2017 - By Mirella Bucci

Nature Chemical Biology 13, 817 (2017). doi:10.1038/nchembio.2444 Author: Mirella…

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Redox regulation: Scaffolding H2O2 signaling

Posted on Jul 18th, 2017 - By Hadley D Sikes

Nature Chemical Biology 13, 818 (2017). doi:10.1038/nchembio.2432 Author: Hadley D Sikes Peroxidases, rather than simply reducing H2O2 to water, also convey oxidation signals to proteins such as transcription factors. A new study reveals how a scaffold protein enables formation of a mixed disulfide between the peroxidase and a transcription…

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Enzymology: Tracking off-targets

Posted on Jul 18th, 2017 - By Alison Farrell

Nature Chemical Biology 13, 817 (2017). doi:10.1038/nchembio.2445 Author: Alison…

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Erratum: The EED protein–protein interaction inhibitor A-395 inactivates the PRC2 complex

Posted on Jul 18th, 2017 - By Yupeng He

Nature Chemical Biology 13, 922 (2017). doi:10.1038/nchembio0817-922b Author: Yupeng He, Sujatha Selvaraju, Michael L Curtin, Clarissa G Jakob, Haizhong Zhu, Kenneth M Comess, Bailin Shaw, Juliana The, Evelyne Lima-Fernandes, Magdalena M Szewczyk, Dong Cheng, Kelly L Klinge, Huan-Qiu Li, Marina Pliushchev, Mikkel A Algire, David Maag, Jun Guo, Justin Dietrich, Sanjay C Panchal, Andrew M Petros, Ramzi F Sweis, Maricel Torrent, Lance J Bigelow, Guillermo Senisterra, Fengling Li,…

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Antibacterials: PUMmeling RNAP

Posted on Jul 18th, 2017 - By Terry L. Sheppard

Nature Chemical Biology 13, 817 (2017). doi:10.1038/nchembio.2446 Author: Terry L.…

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Erratum: Structural and conformational determinants of macrocycle cell permeability

Posted on Jul 18th, 2017 - By Björn Over

Nature Chemical Biology 13, 922 (2017). doi:10.1038/nchembio0817-922a Author: Björn Over, Pär Matsson, Christian Tyrchan, Per Artursson, Bradley C Doak, Michael A Foley, Constanze Hilgendorf, Stephen E Johnston, Maurice D Lee, Richard J Lewis, Patrick McCarren, Giovanni Muncipinto, Ulf Norinder, Matthew W D Perry, Jeremy R Duvall & Jan…

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Biocatalysis: Achieving aminase activity

Posted on Jul 18th, 2017 - By Caitlin Deane

Nature Chemical Biology 13, 817 (2017). doi:10.1038/nchembio.2447 Author: Caitlin…

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Harnessing yeast organelles for metabolic engineering

Posted on Jul 18th, 2017 - By Sarah K Hammer

Nature Chemical Biology 13, 823 (2017). doi:10.1038/nchembio.2429 Authors: Sarah K Hammer & José L…

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An excited state underlies gene regulation of a transcriptional riboswitch

Posted on Jul 17th, 2017 - By Bo Zhao

Nature Chemical Biology 13, 968 (2017). doi:10.1038/nchembio.2427 Authors: Bo Zhao, Sharon L Guffy, Benfeard Williams & Qi…

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Structures of carboxylic acid reductase reveal domain dynamics underlying catalysis

Posted on Jul 17th, 2017 - By Deepankar Gahloth

Nature Chemical Biology 13, 975 (2017). doi:10.1038/nchembio.2434 Authors: Deepankar Gahloth, Mark S Dunstan, Daniela Quaglia, Evaldas Klumbys, Michael P Lockhart-Cairns, Andrew M Hill, Sasha R Derrington, Nigel S Scrutton, Nicholas J Turner & David…

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Allosteric sensitization of proapoptotic BAX

Posted on Jul 10th, 2017 - By Jonathan R Pritz

Nature Chemical Biology 13, 961 (2017). doi:10.1038/nchembio.2433 Authors: Jonathan R Pritz, Franziska Wachter, Susan Lee, James Luccarelli, Thomas E Wales, Daniel T Cohen, Paul Coote, Gregory J Heffron, John R Engen, Walter Massefski & Loren D…

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The structure of vanadium nitrogenase reveals an unusual bridging ligand

Posted on Jul 10th, 2017 - By Daniel Sippel

Nature Chemical Biology 13, 956 (2017). doi:10.1038/nchembio.2428 Authors: Daniel Sippel & Oliver…

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A small-molecule allosteric inhibitor of Mycobacterium tuberculosis tryptophan synthase

Posted on Jul 3rd, 2017 - By Samantha Wellington

Nature Chemical Biology 13, 943 (2017). doi:10.1038/nchembio.2420 Authors: Samantha Wellington, Partha P Nag, Karolina Michalska, Stephen E Johnston, Robert P Jedrzejczak, Virendar K Kaushik, Anne E Clatworthy, Noman Siddiqi, Patrick McCarren, Besnik Bajrami, Natalia I Maltseva, Senya Combs, Stewart L Fisher, Andrzej Joachimiak, Stuart L Schreiber & Deborah T…

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A molecular rheostat maintains ATP levels to drive a synthetic biochemistry system

Posted on Jul 3rd, 2017 - By Paul H Opgenorth

Nature Chemical Biology 13, 938 (2017). doi:10.1038/nchembio.2418 Authors: Paul H Opgenorth, Tyler P Korman, Liviu Iancu & James U…

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Extracellular vesicles are independent metabolic units with asparaginase activity

Posted on Jul 3rd, 2017 - By Nunzio Iraci

Nature Chemical Biology 13, 951 (2017). doi:10.1038/nchembio.2422 Authors: Nunzio Iraci, Edoardo Gaude, Tommaso Leonardi, Ana S H Costa, Chiara Cossetti, Luca Peruzzotti-Jametti, Joshua D Bernstock, Harpreet K Saini, Maurizio Gelati, Angelo Luigi Vescovi, Carlos Bastos, Nuno Faria, Luigi G Occhipinti, Anton J Enright, Christian Frezza & Stefano…

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A new strategy for aromatic ring alkylation in cylindrocyclophane biosynthesis

Posted on Jun 26th, 2017 - By Hitomi Nakamura

Nature Chemical Biology 13, 916 (2017). doi:10.1038/nchembio.2421 Authors: Hitomi Nakamura, Erica E Schultz & Emily P…

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Monitoring thioredoxin redox with a genetically encoded red fluorescent biosensor

Posted on Jun 26th, 2017 - By Yichong Fan

Nature Chemical Biology 13, 1045 (2017). doi:10.1038/nchembio.2417 Authors: Yichong Fan, Merna Makar, Michael X Wang & Hui-wang…

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Molecular basis of the evolution of alternative tyrosine biosynthetic routes in plants

Posted on Jun 26th, 2017 - By Craig A Schenck

Nature Chemical Biology 13, 1029 (2017). doi:10.1038/nchembio.2414 Authors: Craig A Schenck, Cynthia K Holland, Matthew R Schneider, Yusen Men, Soon Goo Lee, Joseph M Jez & Hiroshi A…

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2′-Deoxyadenosine 5′-diphosphoribose is an endogenous TRPM2 superagonist

Posted on Jun 26th, 2017 - By Ralf Fliegert

Nature Chemical Biology 13, 1036 (2017). doi:10.1038/nchembio.2415 Authors: Ralf Fliegert, Andreas Bauche, Adriana-Michelle Wolf Pérez, Joanna M Watt, Monika D Rozewitz, Riekje Winzer, Mareike Janus, Feng Gu, Annette Rosche, Angelika Harneit, Marianne Flato, Christelle Moreau, Tanja Kirchberger, Valerie Wolters, Barry V L Potter & Andreas H…

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Targeting the N terminus for site-selective protein modification

Posted on Jun 20th, 2017 - By Christian B Rosen

Nature Chemical Biology 13, 697 (2017). doi:10.1038/nchembio.2416 Authors: Christian B Rosen & Matthew B…

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Metal homeostasis: Pumping iron

Posted on Jun 20th, 2017 - By Grant Miura

Nature Chemical Biology 13, 693 (2017). doi:10.1038/nchembio.2423 Author: Grant…

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Antimicrobials: Targeting fungal chromatin

Posted on Jun 20th, 2017 - By Stéphane Larochelle

Nature Chemical Biology 13, 693 (2017). doi:10.1038/nchembio.2424 Author: Stéphane…

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Circadian regulation: Switching periods

Posted on Jun 20th, 2017 - By Grant Miura

Nature Chemical Biology 13, 693 (2017). doi:10.1038/nchembio.2426 Author: Grant…

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Synthetic biology: Playing favorites

Posted on Jun 20th, 2017 - By Ross Cloney

Nature Chemical Biology 13, 693 (2017). doi:10.1038/nchembio.2425 Author: Ross…

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Peptidoglycan: Another brick in the wall

Posted on Jun 20th, 2017 - By Anthony J Clarke

Nature Chemical Biology 13, 695 (2017). doi:10.1038/nchembio.2419 Author: Anthony J Clarke Lipid II embodies the bricks used to build the essential bacterial cell wall component peptidoglycan. A facile new procedure for preparation of species-specific Lipid II in high yields can now be used to unlock the door to antibiotic…

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Nucleation and growth of a bacterial functional amyloid at single-fiber resolution

Posted on Jun 19th, 2017 - By Mike Sleutel

Nature Chemical Biology 13, 902 (2017). doi:10.1038/nchembio.2413 Authors: Mike Sleutel, Imke Van den Broeck, Nani Van Gerven, Cécile Feuillie, Wim Jonckheere, Claire Valotteau, Yves F Dufrêne & Han…

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Cpf1 proteins excise CRISPR RNAs from mRNA transcripts in mammalian cells

Posted on Jun 19th, 2017 - By Guocai Zhong

Nature Chemical Biology 13, 839 (2017). doi:10.1038/nchembio.2410 Authors: Guocai Zhong, Haimin Wang, Yujun Li, Mai H Tran & Michael Farzan Cpf1 is a CRISPR effector protein that has greater specificity than Streptococcus pyogenes Cas9 (SpCas9) in genome-editing applications. Here we show that Lachnospiraceae bacterium (Lb) and Acidaminococus sp. (As) Cpf1 orthologs have RNase activities that can excise multiple CRISPR RNAs (crRNAs) from a single RNA polymerase…

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A heme-dependent enzyme forms the nitrogen–nitrogen bond in piperazate

Posted on Jun 19th, 2017 - By Yi-Ling Du

Nature Chemical Biology 13, 836 (2017). doi:10.1038/nchembio.2411 Authors: Yi-Ling Du, Hai-Yan He, Melanie A Higgins & Katherine S Ryan Molecules containing a nitrogen–nitrogen (N–N) linkage have a variety of structures and biological activities; however, no enzyme has yet been demonstrated to catalyze N–N bond formation in an organic molecule. Here we report that the heme-dependent enzyme KtzT from Kutzneria sp. 744 catalyzes N–N bond formation in the…

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A scaffold protein that chaperones a cysteine-sulfenic acid in H2O2 signaling

Posted on Jun 19th, 2017 - By Antoine Bersweiler

Nature Chemical Biology 13, 909 (2017). doi:10.1038/nchembio.2412 Authors: Antoine Bersweiler, Benoît D'Autréaux, Hortense Mazon, Alexandre Kriznik, Gemma Belli, Agnès Delaunay-Moisan, Michel B Toledano & Sophie…

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A water-soluble DsbB variant that catalyzes disulfide-bond formation in vivo

Posted on Jun 19th, 2017 - By Dario Mizrachi

Nature Chemical Biology 13, 1022 (2017). doi:10.1038/nchembio.2409 Authors: Dario Mizrachi, Michael-Paul Robinson, Guoping Ren, Na Ke, Mehmet Berkmen & Matthew P…

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A front-face ‘SNi synthase’ engineered from a retaining ‘double-SN2’ hydrolase

Posted on Jun 12th, 2017 - By Javier Iglesias-Fernández

Nature Chemical Biology 13, 874 (2017). doi:10.1038/nchembio.2394 Authors: Javier Iglesias-Fernández, Susan M Hancock, Seung Seo Lee, Maola Khan, Jo Kirkpatrick, Neil J Oldham, Katherine McAuley, Anthony Fordham-Skelton, Carme Rovira & Benjamin G…

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A scalable platform to identify fungal secondary metabolites and their gene clusters

Posted on Jun 12th, 2017 - By Kenneth D Clevenger

Nature Chemical Biology 13, 895 (2017). doi:10.1038/nchembio.2408 Authors: Kenneth D Clevenger, Jin Woo Bok, Rosa Ye, Galen P Miley, Maria H Verdan, Thomas Velk, Cynthia Chen, KaHoua Yang, Matthew T Robey, Peng Gao, Matthew Lamprecht, Paul M Thomas, Md Nurul Islam, Jonathan M Palmer, Chengcang C Wu, Nancy P Keller & Neil L…

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Site-specific incorporation of phosphotyrosine using an expanded genetic code

Posted on Jun 12th, 2017 - By Christian Hoppmann

Nature Chemical Biology 13, 842 (2017). doi:10.1038/nchembio.2406 Authors: Christian Hoppmann, Allison Wong, Bing Yang, Shuwei Li, Tony Hunter, Kevan M Shokat & Lei Wang Access to phosphoproteins with stoichiometric and site-specific phosphorylation status is key to understanding the role of protein phosphorylation. Here we report an efficient method to generate pure, active phosphotyrosine-containing proteins by genetically encoding a stable phosphotyrosine analog that is…

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Oxidation of phosphorothioate DNA modifications leads to lethal genomic instability

Posted on Jun 12th, 2017 - By Stefanie Kellner

Nature Chemical Biology 13, 888 (2017). doi:10.1038/nchembio.2407 Authors: Stefanie Kellner, Michael S DeMott, Ching Pin Cheng, Brandon S Russell, Bo Cao, Delin You & Peter C…

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Genetically encoding phosphotyrosine and its nonhydrolyzable analog in bacteria

Posted on Jun 12th, 2017 - By Xiaozhou Luo

Nature Chemical Biology 13, 845 (2017). doi:10.1038/nchembio.2405 Authors: Xiaozhou Luo, Guangsen Fu, Rongsheng E Wang, Xueyong Zhu, Claudio Zambaldo, Renhe Liu, Tao Liu, Xiaoxuan Lyu, Jintang Du, Weimin Xuan, Anzhi Yao, Sean A Reed, Mingchao Kang, Yuhan Zhang, Hui Guo, Chunhui Huang, Peng-Yu Yang, Ian A Wilson, Peter G Schultz & Feng…

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A mutant O-GlcNAcase enriches Drosophila developmental regulators

Posted on Jun 12th, 2017 - By Nithya Selvan

Nature Chemical Biology 13, 882 (2017). doi:10.1038/nchembio.2404 Authors: Nithya Selvan, Ritchie Williamson, Daniel Mariappa, David G Campbell, Robert Gourlay, Andrew T Ferenbach, Tonia Aristotelous, Iva Hopkins-Navratilova, Matthias Trost & Daan M F van…

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Blocking an N-terminal acetylation–dependent protein interaction inhibits an E3 ligase

Posted on Jun 5th, 2017 - By Daniel C Scott

Nature Chemical Biology 13, 850 (2017). doi:10.1038/nchembio.2386 Authors: Daniel C Scott, Jared T Hammill, Jaeki Min, David Y Rhee, Michele Connelly, Vladislav O Sviderskiy, Deepak Bhasin, Yizhe Chen, Su-Sien Ong, Sergio C Chai, Asli N Goktug, Guochang Huang, Julie K Monda, Jonathan Low, Ho Shin Kim, Joao A Paulo, Joe R Cannon, Anang A Shelat, Taosheng Chen, Ian R Kelsall, Arno F Alpi, Vishwajeeth Pagala, Xusheng Wang, Junmin Peng, Bhuvanesh Singh, J Wade Harper, Brenda A…

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Activity-based probes for functional interrogation of retaining β-glucuronidases

Posted on Jun 5th, 2017 - By Liang Wu

Nature Chemical Biology 13, 867 (2017). doi:10.1038/nchembio.2395 Authors: Liang Wu, Jianbing Jiang, Yi Jin, Wouter W Kallemeijn, Chi-Lin Kuo, Marta Artola, Wei Dai, Cas van Elk, Marco van Eijk, Gijsbert A van der Marel, Jeroen D C Codée, Bogdan I Florea, Johannes M F G Aerts, Herman S Overkleeft & Gideon J…

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Parallel evolution of non-homologous isofunctional enzymes in methionine biosynthesis

Posted on Jun 5th, 2017 - By Karine Bastard

Nature Chemical Biology 13, 858 (2017). doi:10.1038/nchembio.2397 Authors: Karine Bastard, Alain Perret, Aline Mariage, Thomas Bessonnet, Agnès Pinet-Turpault, Jean-Louis Petit, Ekaterina Darii, Pascal Bazire, Carine Vergne-Vaxelaire, Clémence Brewee, Adrien Debard, Virginie Pellouin, Marielle Besnard-Gonnet, François Artiguenave, Claudine Médigue, David Vallenet, Antoine Danchin, Anne Zaparucha, Jean Weissenbach, Marcel Salanoubat & Véronique de…

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Autocatalytic backbone N-methylation in a family of ribosomal peptide natural products

Posted on Jun 5th, 2017 - By Niels S van der Velden

Nature Chemical Biology 13, 833 (2017). doi:10.1038/nchembio.2393 Authors: Niels S van der Velden, Noemi Kälin, Maximilian J Helf, Jörn Piel, Michael F Freeman & Markus Künzler Peptide backbone N-methylation, as seen in cyclosporin A, has been considered to be exclusive to nonribosomal peptides. We have identified the first post-translationally modified peptide or protein harboring internal α-N-methylations through discovery of the genetic locus for the…

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Structural and functional characterization of the hydrogenase-maturation HydF protein

Posted on May 29th, 2017 - By Giorgio Caserta

Nature Chemical Biology 13, 779 (2017). doi:10.1038/nchembio.2385 Authors: Giorgio Caserta, Ludovic Pecqueur, Agnieszka Adamska-Venkatesh, Cecilia Papini, Souvik Roy, Vincent Artero, Mohamed Atta, Edward Reijerse, Wolfgang Lubitz & Marc…

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Functional selectivity of GPCR-directed drug action through location bias

Posted on May 29th, 2017 - By Roshanak Irannejad

Nature Chemical Biology 13, 799 (2017). doi:10.1038/nchembio.2389 Authors: Roshanak Irannejad, Veronica Pessino, Delphine Mika, Bo Huang, Philip B Wedegaertner, Marco Conti & Mark von…

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Targeting S-adenosylmethionine biosynthesis with a novel allosteric inhibitor of Mat2A

Posted on May 29th, 2017 - By Casey L Quinlan

Nature Chemical Biology 13, 785 (2017). doi:10.1038/nchembio.2384 Authors: Casey L Quinlan, Stephen E Kaiser, Ben Bolaños, Dawn Nowlin, Rita Grantner, Shannon Karlicek-Bryant, Jun Li Feng, Stephen Jenkinson, Kevin Freeman-Cook, Stephen G Dann, Xiaoli Wang, Peter A Wells, Valeria R Fantin, Al E Stewart & Stephan K…

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Structural basis for high-affinity fluorophore binding and activation by RNA Mango

Posted on May 29th, 2017 - By Robert J Trachman

Nature Chemical Biology 13, 807 (2017). doi:10.1038/nchembio.2392 Authors: Robert J Trachman, Natalia A Demeshkina, Matthew W L Lau, Shanker Shyam S Panchapakesan, Sunny C Y Jeng, Peter J Unrau & Adrian R…

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Lipid II overproduction allows direct assay of transpeptidase inhibition by β-lactams

Posted on May 29th, 2017 - By Yuan Qiao

Nature Chemical Biology 13, 793 (2017). doi:10.1038/nchembio.2388 Authors: Yuan Qiao, Veerasak Srisuknimit, Frederick Rubino, Kaitlin Schaefer, Natividad Ruiz, Suzanne Walker & Daniel…

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Engineering RGB color vision into Escherichia coli

Posted on May 22nd, 2017 - By Jesus Fernandez-Rodriguez

Nature Chemical Biology 13, 706 (2017). doi:10.1038/nchembio.2390 Authors: Jesus Fernandez-Rodriguez, Felix Moser, Miryoung Song & Christopher A Voigt Optogenetic tools use colored light to rapidly control gene expression in space and time. We designed a genetically encoded system that gives Escherichia coli the ability to distinguish between red, green, and blue (RGB) light and respond by changing gene expression. We use this system to produce 'color photographs' on…

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Engineering protein stability with atomic precision in a monomeric miniprotein

Posted on May 22nd, 2017 - By Emily G Baker

Nature Chemical Biology 13, 764 (2017). doi:10.1038/nchembio.2380 Authors: Emily G Baker, Christopher Williams, Kieran L Hudson, Gail J Bartlett, Jack W Heal, Kathryn L Porter Goff, Richard B Sessions, Matthew P Crump & Derek N…

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A combinatorial screen of the CLOUD uncovers a synergy targeting the androgen receptor

Posted on May 22nd, 2017 - By Marco P Licciardello

Nature Chemical Biology 13, 771 (2017). doi:10.1038/nchembio.2382 Authors: Marco P Licciardello, Anna Ringler, Patrick Markt, Freya Klepsch, Charles-Hugues Lardeau, Sara Sdelci, Erika Schirghuber, André C Müller, Michael Caldera, Anja Wagner, Rebecca Herzog, Thomas Penz, Michael Schuster, Bernd Boidol, Gerhard Dürnberger, Yasin Folkvaljon, Pär Stattin, Vladimir Ivanov, Jacques Colinge, Christoph Bock, Klaus Kratochwill, Jörg Menche, Keiryn L Bennett &…

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Recognition of EGF-like domains by the Notch-modifying O-fucosyltransferase POFUT1

Posted on May 22nd, 2017 - By Zhijie Li

Nature Chemical Biology 13, 757 (2017). doi:10.1038/nchembio.2381 Authors: Zhijie Li, Kristina Han, John E Pak, Malathy Satkunarajah, Dongxia Zhou & James M…

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Erratum: A Vibrio cholerae autoinducer–receptor pair that controls biofilm formation

Posted on May 17th, 2017 - By Kai Papenfort

Nature Chemical Biology 13, 691 (2017). doi:10.1038/nchembio0617-691a Author: Kai Papenfort, Justin E Silpe, Kelsey R Schramma, Jian-Ping Cong, Mohammad R Seyedsayamdost & Bonnie L…

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Optogenetics: Switching with red and blue

Posted on May 17th, 2017 - By Fuun Kawano

Nature Chemical Biology 13, 573 (2017). doi:10.1038/nchembio.2387 Authors: Fuun Kawano, Fan Shi & Masayuki Yazawa Multiple optogenetic technologies are required to control biological activity simultaneously with different colors of light. Optimizing a near-infrared-induced heterodimerization system, which can be combined with blue-light-controlled domains, enables precise spatiotemporal control of target molecules in live mammalian…

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Small-molecule pheromones and hormones controlling nematode development

Posted on May 17th, 2017 - By Rebecca A Butcher

Nature Chemical Biology 13, 577 (2017). doi:10.1038/nchembio.2356 Author: Rebecca A…

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Erratum: Full antagonism of the estrogen receptor without a prototypical ligand side chain

Posted on May 17th, 2017 - By Sathish Srinivasan

Nature Chemical Biology 13, 691 (2017). doi:10.1038/nchembio0617-691b Author: Sathish Srinivasan, Jerome C Nwachukwu, Nelson E Bruno, Venkatasubramanian Dharmarajan, Devrishi Goswami, Irida Kastrati, Scott Novick, Jason Nowak, Valerie Cavett, Hai-Bing Zhou, Nittaya Boonmuen, Yuechao Zhao, Jian Min, Jonna Frasor, Benita S Katzenellenbogen, Patrick R Griffin, John A Katzenellenbogen & Kendall W…

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Illuminating developmental biology through photochemistry

Posted on May 17th, 2017 - By Lukasz Kowalik

Nature Chemical Biology 13, 587 (2017). doi:10.1038/nchembio.2369 Authors: Lukasz Kowalik & James K…

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Small-molecule phenotypic screening with stem cells

Posted on May 17th, 2017 - By Andrei Ursu

Nature Chemical Biology 13, 560 (2017). doi:10.1038/nchembio.2383 Authors: Andrei Ursu, Hans R Schöler & Herbert Waldmann To fully leverage the potential of human-induced pluripotent stem cells (hiPSCs), improved and standardized reprogramming methods and large-scale collections of hiPSC lines are needed, and the stem cell community must embrace chemical biology methodology for target identification and…

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The perception of strigolactones in vascular plants

Posted on May 17th, 2017 - By Shelley Lumba

Nature Chemical Biology 13, 599 (2017). doi:10.1038/nchembio.2340 Authors: Shelley Lumba, Duncan Holbrook-Smith & Peter…

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Infectious disease: A lethal sugar fix

Posted on May 17th, 2017 - By Alison Farrell

Nature Chemical Biology 13, 569 (2017). doi:10.1038/nchembio.2400 Author: Alison…

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Shaping embryonic development

Nature Chemical Biology 13, 559 (2017). doi:10.1038/nchembio.2403 The growing intersection between chemical tools and principles and developmental biology is providing new insights into the molecular-level details of developmental…

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Unraveling cell-to-cell signaling networks with chemical biology

Posted on May 17th, 2017 - By Zev J Gartner

Nature Chemical Biology 13, 564 (2017). doi:10.1038/nchembio.2391 Authors: Zev J Gartner, Jennifer A Prescher & Luke D Lavis Cell-to-cell signaling networks, although poorly understood, guide tissue development, regulate tissue function and may become dysregulated in disease. Chemical biologists can develop the next generation of tools to untangle these complex and dynamic networks of interacting…

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Corrigendum: Full antagonism of the estrogen receptor without a prototypical ligand side chain

Posted on May 17th, 2017 - By Sathish Srinivasan

Nature Chemical Biology 13, 691 (2017). doi:10.1038/nchembio0617-691c Author: Sathish Srinivasan, Jerome C Nwachukwu, Nelson E Bruno, Venkatasubramanian Dharmarajan, Devrishi Goswami, Irida Kastrati, Scott Novick, Jason Nowak, Valerie Cavett, Hai-Bing Zhou, Nittaya Boonmuen, Yuechao Zhao, Jian Min, Jonna Frasor, Benita S Katzenellenbogen, Patrick R Griffin, John A Katzenellenbogen & Kendall W…

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Enzymology: Radical ring resizing

Posted on May 17th, 2017 - By Caitlin Deane

Nature Chemical Biology 13, 569 (2017). doi:10.1038/nchembio.2399 Author: Caitlin…

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Synthetic biology: Return to sender

Posted on May 17th, 2017 - By Grant Miura

Nature Chemical Biology 13, 569 (2017). doi:10.1038/nchembio.2398 Author: Grant…

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