[ASAP] Living Therapeutics: The Next Frontier of Precision Medicine

[ASAP] An Automated Model Test System for Systematic Development and Improvement of Gene Expression Models

Posted on Oct 14th, 2020 - By Alexander C. Reis and Howard M. Salis

[ASAP] Isothermal SARS-CoV-2 Diagnostics: Tools for Enabling Distributed Pandemic Testing as a Means of Supporting Safe Reopenings

[ASAP] Reading and Writing Digital Information in TNA

[ASAP] Conditional Protein Rescue by Binding-Induced Protective Shielding

Posted on Oct 6th, 2020 - By Andrew S. Gaynor and Wilfred Chen

[ASAP] Reconstitution of the Ornithine Cycle with Arginine:Glycine Amidinotransferase to Engineer <italic toggle=”yes”>Escherichia coli</italic> into an Efficient Whole-Cell Catalyst of Guanidinoacetate

Posted on Aug 6th, 2020 - By pthomas2

[ASAP] Engineering <italic toggle=”yes”>Yarrowia lipolytica</italic> as a Chassis for <italic toggle=”yes”>De Novo</italic> Synthesis of Five Aromatic-Derived Natural Products and Chemicals

Posted on Jul 22nd, 2020 - By pthomas2

[ASAP] Programmed Allelic Mutagenesis of a DNA Polymerase with Single Amino Acid Resolution

Posted on Jun 29th, 2020 - By pthomas2

[ASAP] Artificial Protein-Responsive Riboswitches Upregulate Non-AUG Translation Initiation in Yeast

Posted on Jun 22nd, 2020 - By Fumihiro Horie, Kei Endo*, and Koichi Ito*

[ASAP] Harnessing Natural Modularity of Metabolism with Goal Attainment Optimization to Design a Modular Chassis Cell for Production of Diverse Chemicals

Posted on Jun 22nd, 2020 - By Sergio Garcia†‡ and Cong T. Trinh*†‡

[ASAP] CRISPRi-dCas12a: A dCas12a-Mediated CRISPR Interference for Repression of Multiple Genes and Metabolic Engineering in Cyanobacteria

Posted on May 18th, 2020 - By Sun Young Choi†‡ and Han Min Woo*†‡

[ASAP] Translation Related Factors Improve the Productivity of a <italic toggle=”yes”>Streptomyces</italic>-Based Cell-Free Protein Synthesis System

Posted on May 5th, 2020 - By Huiling Xu, Wan-Qiu Liu, and Jian Li*

[ASAP] The Protection Role of Magnesium Ions on Coupled Transcription and Translation in Lyophilized Cell-Free System

[ASAP] Yeast Surface Display System for Facilitated Production and Application of Phage Endolysin

Posted on Mar 10th, 2020 - By pthomas2

[ASAP] MEMO: A Method for Computing Metabolic Modules for Cell-Free Production Systems

Posted on Mar 2nd, 2020 - By Axel von Kamp† and Steffen Klamt*†

[ASAP] A New Biosensor for Stilbenes and a Cannabinoid Enabled by Genome Mining of a Transcriptional Regulator

[ASAP] Development of a Quorum-Sensing Based Circuit for Control of Coculture Population Composition in a Naringenin Production System

[ASAP] Highly Efficient Biosynthesis of Hypoxanthine in <italic toggle=”yes”>Escherichia coli</italic> and Transcriptome-Based Analysis of the Purine Metabolism

[ASAP] Mitochondrial Compartmentalization Confers Specificity to the 2-Ketoacid Recursive Pathway: Increasing Isopentanol Production in <italic toggle=”yes”>Saccharomyces cerevisiae</italic>

[ASAP] Engineering a DNAzyme-Based Operon System for the Production of DNA Nanoscaffolds in Living Bacteria

[ASAP] Reinforcement Learning for Bioretrosynthesis

Posted on Dec 30th, 2019 - By pthomas2

[ASAP] Tunable Repression of Key Photosynthetic Processes Using Cas12a CRISPR Interference in the Fast-Growing Cyanobacterium <italic toggle=”yes”>Synechococcus</italic> sp. UTEX 2973

[ASAP] Orthogonal Protein-Responsive mRNA Switches for Mammalian Synthetic Biology

Posted on Dec 13th, 2019 - By pthomas2

[ASAP] Unravelling the Biosynthetic Flexibility of UK-2A Enables Enzymatic Synthesis of Its Structural Variants

Posted on Nov 25th, 2019 - By pthomas2

[ASAP] Enhanced Population Control in a Synthetic Bacterial Consortium by Interconnected Carbon Cross-Feeding

[ASAP] Interkingdom Genetic Mix-and-Match To Produce Novel Sunscreens

Posted on Nov 7th, 2019 - By Andrew R. Osborn and Taifo Mahmud*

[ASAP] <italic toggle=”yes”>In Vitro</italic> Transcriptional Regulation <italic toggle=”yes”>via</italic> Nucleic-Acid-Based Transcription Factors

[ASAP] Improving Chromosome Synthesis with a Semiquantitative Phenotypic Assay and Refined Assembly Strategy

Posted on Sep 19th, 2019 - By pthomas2

[ASAP] Low-Burden Biological Feedback Controllers for Near-Perfect Adaptation

Posted on Sep 12th, 2019 - By Harrison Steel* and Antonis Papachristodoulou*

[ASAP] Modular Thermal Control of Protein Dimerization

[ASAP] Recombination-Independent Genome Editing through CRISPR/Cas9-Enhanced TargeTron Delivery

[ASAP] Development of a Vanillate Biosensor for the Vanillin Biosynthesis Pathway in <italic toggle=”yes”>E. coli</italic>

[ASAP] A Model for the Spatiotemporal Design of Gene Regulatory Circuits<xref rid=”notes-00″ ref-type=”statement”>†</xref>

[ASAP] Construction of Genetic Logic Gates Based on the T7 RNA Polymerase Expression System in <italic toggle=”yes”>Rhodococcus opacus</italic> PD630

Posted on Aug 6th, 2019 - By Drew M. DeLorenzo† and Tae Seok Moon*†‡

[ASAP] Genetic Selection for Small Molecule Production in Competitive Microfluidic Droplets

Posted on Aug 4th, 2019 - By pthomas2

[ASAP] Reversing an Extracellular Electron Transfer Pathway for Electrode-Driven Acetoin Reduction

[ASAP] Fluorescence-Activated Droplet Sorting for Single-Cell Directed Evolution

[ASAP] Genetic Compatibility and Extensibility of Orthogonal Replication

Posted on May 21st, 2019 - By Alex A. Javanpour, Chang C. Liu

[ASAP] Elucidating the Determinants of Polymerase Specificity by Microfluidic-Based Deep Mutational Scanning

Posted on May 12th, 2019 - By Ali Nikoomanzar, Derek Vallejo, John C. Chaput

[ASAP] RNA-Catalyzed Polymerization of Deoxyribose, Threose, and Arabinose Nucleic Acids

[ASAP] Synthetic Bistability and Differentiation in Yeast

Posted on Apr 30th, 2019 - By Yaoyu Yang, Jennifer L. Nemhauser, Eric Klavins

[ASAP] Combinatorial Sensor Design in <italic toggle=”yes”>Caulobacter crescentus</italic> for Selective Environmental Uranium Detection

Posted on Apr 2nd, 2019 - By Dan M. Park, Michael J. Taffet

[ASAP] Building an Inducible T7 RNA Polymerase/T7 Promoter Circuit in <italic toggle=”yes”>Synechocystis</italic> sp. PCC6803

Posted on Apr 2nd, 2019 - By Haojie Jin, Peter Lindblad, Devaki Bhaya

[ASAP] Systematic Evaluation of Genetic and Environmental Factors Affecting Performance of Translational Riboswitches

Posted on Apr 1st, 2019 - By R. Kent, N. Dixon

[ASAP] An Accessible Continuous-Culture Turbidostat for Pooled Analysis of Complex Libraries

[ASAP] Modeling Genetic Circuit Behavior in Transiently Transfected Mammalian Cells

Posted on Mar 27th, 2019 - By Junmin Wang, Samuel A. Isaacson, Calin Belta

[ASAP] Enhanced Pyruvate Production in <italic toggle=”yes”>Candida glabrata</italic> by Engineering ATP Futile Cycle System

[ASAP] Structural Characterization of a Synthetic Tandem-Domain Bacterial Microcompartment Shell Protein Capable of Forming Icosahedral Shell Assemblies

[ASAP] Spatiotemporal Gene Repression System in the Heterocyst-Forming Multicellular Cyanobacterium <italic toggle=”yes”>Anabaena</italic> sp. PCC 7120

Posted on Mar 18th, 2019 - By Akiyoshi Higo, Shigeki Ehira

[ASAP] Desymmetrization of Cyclodepsipeptides by Assembly Mode Switching of Iterative Nonribosomal Peptide Synthetases

[ASAP] Measuring Amber Initiator tRNA Orthogonality in a Genomically Recoded Organism

[ASAP] Temporal Pattern Recognition through Analog Molecular Computation

Posted on Mar 13th, 2019 - By Jackson O’Brien, Arvind Murugan

[ASAP] Construction of Incoherent Feedforward Loop Circuits in a Cell-Free System and in Cells

Posted on Mar 6th, 2019 - By Shaobin Guo, Richard M. Murray

[ASAP] Metabolic Engineering of a Homoserine-Derived Non-Natural Pathway for the <italic toggle=”yes”>De Novo</italic> Production of 1,3-Propanediol from Glucose

[ASAP] Synthetic Inducible Regulatory Systems Optimized for the Modulation of Secondary Metabolite Production in <italic toggle=”yes”>Streptomyces</italic>

Posted on Mar 4th, 2019 - By Chang-Hun Ji, Hiyoung Kim, Hahk-Soo Kang

[ASAP] Physical Plasma Membrane Perturbation Using Subcellular Optogenetics Drives Integrin-Activated Cell Migration

Posted on Feb 22nd, 2019 - By Xenia Meshik, Patrick R. O’Neill, N. Gautam

[ASAP] Combining 26s rDNA and the Cre-loxP System for Iterative Gene Integration and Efficient Marker Curation in <italic toggle=”yes”>Yarrowia lipolytica</italic>

[ASAP] Redirecting Metabolic Flux <italic toggle=”yes”>via</italic> Combinatorial Multiplex CRISPRi-Mediated Repression for Isopentenol Production in <italic toggle=”yes”>Escherichia coli</italic>

[ASAP] Antisense RNA-Based Strategy for Enhancing Surfactin Production in <italic toggle=”yes”>Bacillus subtilis</italic> TS1726 via Overexpression of the Unconventional Biotin Carboxylase II To Enhance ACCase Activity

Posted on Jan 31st, 2019 - By Miaomiao Wang, Huimin Yu, Zhongyao Shen

[ASAP] Engineering a New Class of Anti-LacI Transcription Factors with Alternate DNA Recognition

Posted on Jan 20th, 2019 - By Ronald E. Rondon, Corey J. Wilson

[ASAP] Synthetic Transcription Factors Switch from Local to Long-Range Control during Cell Differentiation

Posted on Jan 17th, 2019 - By Takeo Wada, Sandrine Wallerich, Attila Becskei

[ASAP] An Artificial Pathway for Isoprenoid Biosynthesis Decoupled from Native Hemiterpene Metabolism

Posted on Jan 16th, 2019 - By Sean Lund, Rachael Hall, Gavin J Williams

[ASAP] Metabolic Engineering of <italic toggle=”yes”>Saccharomyces cerevisiae</italic> for Production of Shinorine, a Sunscreen Material, from Xylose

[ASAP] Biological Engineered Living Materials: Growing Functional Materials with Genetically Programmable Properties

Posted on Jan 9th, 2019 - By Charlie Gilbert, Tom Ellis

[ASAP] Structure-Based Design of Versatile Biosensors for Small Molecules Based on the PAS Domain of a Thermophilic Histidine Kinase

Posted on Dec 10th, 2018 - By Kai U. Cormann, Meike Baumgart, Michael Bott

[ASAP] Retroelement-Based Genome Editing and Evolution

[ASAP] Transcription Driven by Reversible Photocontrol of Hyperstable G-Quadruplexes

Posted on Oct 16th, 2018 - By Shinzi Ogasawara

[ASAP] Enhancing Light-Driven 1,3-Propanediol Production by Using Natural Compartmentalization of Differentiated Cells

Posted on Sep 19th, 2018 - By Hongyu Liu, Jun Ni, Ping Xu, Fei Tao

[ASAP] Optimization of Artificial Curcumin Biosynthesis in <italic toggle=”yes”>E. coli</italic> by Randomized 5′-UTR Sequences To Control the Multienzyme Pathway

Posted on Sep 3rd, 2018 - By Sun-Young Kang, Kyung Taek Heo, Young-Soo Hong

[ASAP] Tamoxifen- and Mifepristone-Inducible Versions of CRISPR Effectors, Cas9 and Cpf1

Posted on Sep 3rd, 2018 - By Alazne Dominguez-Monedero, Jamie A. Davies

[ASAP] <italic toggle=”yes”>In Vitro</italic> Transcription Networks Based on Hairpin Promoter Switches

Posted on Jul 31st, 2018 - By Shaunak Kar, Andrew D. Ellington

[ASAP] Robustness of a Reconstituted <italic toggle=”yes”>Escherichia coli</italic> Protein Translation System Analyzed by Computational Modeling

[ASAP] Tight Translational Control Using Site-Specific Unnatural Amino Acid Incorporation with Positive Feedback Gene Circuits

Posted on Jul 19th, 2018 - By Yusuke Kato

[ASAP] Drug-Inducible Control of Lethality Genes: A Low Background Destabilizing Domain Architecture Applied to the Gal4-UAS System in <italic toggle=”yes”>Drosophila</italic>

Posted on May 14th, 2018 - By Manjunatha Kogenaru, Mark Isalan

[ASAP] A Dynamic Model of Resource Allocation in Response to the Presence of a Synthetic Construct

[ASAP] Modularization and Response Curve Engineering of a Naringenin-Responsive Transcriptional Biosensor

Wicket: A Versatile Tool for the Integration and Optimization of Exogenous Pathways in Saccharomyces cerevisiae

Posted on Feb 28th, 2018 - By Sha Hou, Qin Qin and Junbiao Dai

Directed Evolution of Membrane Transport Using Synthetic Selections

Orthogonal Information Encoding in Living Cells with High Error-Tolerance, Safety, and Fidelity

Posted on Feb 21st, 2018 - By Lifu Song and An-Ping Zeng

Genetically Encoded Circuit for Remote Regulation of Cell Migration by Magnetic Fields

Posted on Jan 26th, 2018 - By Abdullah A. Mosabbir and Kevin Truong

Stringency of Synthetic Promoter Sequences in Clostridium Revealed and Circumvented by Tuning Promoter Library Mutation Rates

Posted on Jan 26th, 2018 - By Paweł M. Mordaka and John T. Heap

Analyzing and Tuning Ribozyme Activity by Deep Sequencing To Modulate Gene Expression Level in Mammalian Cells

Posted on Jan 24th, 2018 - By Shungo Kobori and Yohei Yokobayashi

MinGenome: An In Silico Top-Down Approach for the Synthesis of Minimized Genomes

Posted on Jan 20th, 2018 - By Lin Wang and Costas D. Maranas

Metabolic Feedback Circuits Provide Rapid Control of Metabolite Dynamics

Posted on Jan 8th, 2018 - By Di Liu and Fuzhong Zhang

RNA Thermometers for the PURExpress System

Development of a Pigment-Based Whole-Cell Zinc Biosensor for Human Serum

Posted on Dec 22nd, 2017 - By Daniel M. Watstein and Mark P. Styczynski

A Genetic Toolbox for Modulating the Expression of Heterologous Genes in the Cyanobacterium Synechocystis sp. PCC 6803

Cell-Free and In Vivo Characterization of Lux, Las, and Rpa Quorum Activation Systems in E. coli

Posted on Nov 28th, 2017 - By Andrew D. Halleran and Richard M. Murray

Yield Improvement of the Anti-MRSA Antibiotics WAP-8294A by CRISPR/dCas9 Combined with Refactoring Self-Protection Genes in Lysobacter enzymogenes OH11

Controlling Bdellovibrio bacteriovorus Gene Expression and Predation Using Synthetic Riboswitches

Posted on Aug 18th, 2017 - By Mohammed Dwidar and Yohei Yokobayashi

Enabling Graded and Large-Scale Multiplex of Desired Genes Using a Dual-Mode dCas9 Activator in Saccharomyces cerevisiae

Posted on Jul 27th, 2017 - By Matthew Deaner, Julio Mejia and Hal S. Alper

Unique Thermal Stability of Unnatural Hydrophobic Ds Bases in Double-Stranded DNAs

Posted on Jul 26th, 2017 - By Michiko Kimoto and Ichiro Hirao

Developing a Genetically Encoded, Cross-Species Biosensor for Detecting Ammonium and Regulating Biosynthesis of Cyanophycin

Posted on Jul 13th, 2017 - By Yi Xiao, Wen Jiang and Fuzhong Zhang

CAGE: Chromatin Analogous Gene Expression

Posted on Jun 30th, 2017 - By Reza M. Zadegan and William L. Hughes

De Novo Biosynthesis of Glutarate via α-Keto Acid Carbon Chain Extension and Decarboxylation Pathway in Escherichia coli

Autonomous Cell Migration to CSF1 Sources via a Synthetic Protein-Based System

Posted on May 10th, 2017 - By Anam Qudrat and Kevin Truong

Deep Sequencing Analysis of Aptazyme Variants Based on a Pistol Ribozyme

Measurement of In Vivo Protein Binding Affinities in a Signaling Network with Mass Spectrometry

A Glucose-Sensing Toggle Switch for Autonomous, High Productivity Genetic Control

A Secretion-Amplification Role for Salmonella enterica Translocon Protein SipD