[ASAP] Structure-Based Design of Versatile Biosensors for Small Molecules Based on the PAS Domain of a Thermophilic Histidine Kinase

Posted on Dec 10th, 2018 - By Kai U. Cormann, Meike Baumgart, Michael Bott

[ASAP] Retroelement-Based Genome Editing and Evolution

[ASAP] Transcription Driven by Reversible Photocontrol of Hyperstable G-Quadruplexes

Posted on Oct 16th, 2018 - By Shinzi Ogasawara

[ASAP] Enhancing Light-Driven 1,3-Propanediol Production by Using Natural Compartmentalization of Differentiated Cells

Posted on Sep 19th, 2018 - By Hongyu Liu, Jun Ni, Ping Xu, Fei Tao

[ASAP] Optimization of Artificial Curcumin Biosynthesis in <italic toggle=”yes”>E. coli</italic> by Randomized 5′-UTR Sequences To Control the Multienzyme Pathway

Posted on Sep 3rd, 2018 - By Sun-Young Kang, Kyung Taek Heo, Young-Soo Hong

[ASAP] Tamoxifen- and Mifepristone-Inducible Versions of CRISPR Effectors, Cas9 and Cpf1

Posted on Sep 3rd, 2018 - By Alazne Dominguez-Monedero, Jamie A. Davies

[ASAP] <italic toggle=”yes”>In Vitro</italic> Transcription Networks Based on Hairpin Promoter Switches

Posted on Jul 31st, 2018 - By Shaunak Kar, Andrew D. Ellington

[ASAP] Robustness of a Reconstituted <italic toggle=”yes”>Escherichia coli</italic> Protein Translation System Analyzed by Computational Modeling

[ASAP] Tight Translational Control Using Site-Specific Unnatural Amino Acid Incorporation with Positive Feedback Gene Circuits

Posted on Jul 19th, 2018 - By Yusuke Kato

[ASAP] Drug-Inducible Control of Lethality Genes: A Low Background Destabilizing Domain Architecture Applied to the Gal4-UAS System in <italic toggle=”yes”>Drosophila</italic>

Posted on May 14th, 2018 - By Manjunatha Kogenaru, Mark Isalan

[ASAP] A Dynamic Model of Resource Allocation in Response to the Presence of a Synthetic Construct

[ASAP] Modularization and Response Curve Engineering of a Naringenin-Responsive Transcriptional Biosensor

Wicket: A Versatile Tool for the Integration and Optimization of Exogenous Pathways in Saccharomyces cerevisiae

Posted on Feb 28th, 2018 - By Sha Hou, Qin Qin and Junbiao Dai

Directed Evolution of Membrane Transport Using Synthetic Selections

Orthogonal Information Encoding in Living Cells with High Error-Tolerance, Safety, and Fidelity

Posted on Feb 21st, 2018 - By Lifu Song and An-Ping Zeng

Genetically Encoded Circuit for Remote Regulation of Cell Migration by Magnetic Fields

Posted on Jan 26th, 2018 - By Abdullah A. Mosabbir and Kevin Truong

Stringency of Synthetic Promoter Sequences in Clostridium Revealed and Circumvented by Tuning Promoter Library Mutation Rates

Posted on Jan 26th, 2018 - By Paweł M. Mordaka and John T. Heap

Analyzing and Tuning Ribozyme Activity by Deep Sequencing To Modulate Gene Expression Level in Mammalian Cells

Posted on Jan 24th, 2018 - By Shungo Kobori and Yohei Yokobayashi

MinGenome: An In Silico Top-Down Approach for the Synthesis of Minimized Genomes

Posted on Jan 20th, 2018 - By Lin Wang and Costas D. Maranas

Metabolic Feedback Circuits Provide Rapid Control of Metabolite Dynamics

Posted on Jan 8th, 2018 - By Di Liu and Fuzhong Zhang

RNA Thermometers for the PURExpress System

Development of a Pigment-Based Whole-Cell Zinc Biosensor for Human Serum

Posted on Dec 22nd, 2017 - By Daniel M. Watstein and Mark P. Styczynski

A Genetic Toolbox for Modulating the Expression of Heterologous Genes in the Cyanobacterium Synechocystis sp. PCC 6803

Cell-Free and In Vivo Characterization of Lux, Las, and Rpa Quorum Activation Systems in E. coli

Posted on Nov 28th, 2017 - By Andrew D. Halleran and Richard M. Murray

Yield Improvement of the Anti-MRSA Antibiotics WAP-8294A by CRISPR/dCas9 Combined with Refactoring Self-Protection Genes in Lysobacter enzymogenes OH11

Controlling Bdellovibrio bacteriovorus Gene Expression and Predation Using Synthetic Riboswitches

Posted on Aug 18th, 2017 - By Mohammed Dwidar and Yohei Yokobayashi

Enabling Graded and Large-Scale Multiplex of Desired Genes Using a Dual-Mode dCas9 Activator in Saccharomyces cerevisiae

Posted on Jul 27th, 2017 - By Matthew Deaner, Julio Mejia and Hal S. Alper

Unique Thermal Stability of Unnatural Hydrophobic Ds Bases in Double-Stranded DNAs

Posted on Jul 26th, 2017 - By Michiko Kimoto and Ichiro Hirao

Developing a Genetically Encoded, Cross-Species Biosensor for Detecting Ammonium and Regulating Biosynthesis of Cyanophycin

Posted on Jul 13th, 2017 - By Yi Xiao, Wen Jiang and Fuzhong Zhang

CAGE: Chromatin Analogous Gene Expression

Posted on Jun 30th, 2017 - By Reza M. Zadegan and William L. Hughes

De Novo Biosynthesis of Glutarate via α-Keto Acid Carbon Chain Extension and Decarboxylation Pathway in Escherichia coli

Autonomous Cell Migration to CSF1 Sources via a Synthetic Protein-Based System

Posted on May 10th, 2017 - By Anam Qudrat and Kevin Truong

Deep Sequencing Analysis of Aptazyme Variants Based on a Pistol Ribozyme

Measurement of In Vivo Protein Binding Affinities in a Signaling Network with Mass Spectrometry

A Glucose-Sensing Toggle Switch for Autonomous, High Productivity Genetic Control

A Secretion-Amplification Role for Salmonella enterica Translocon Protein SipD

A Modular Toolkit for Generating Pichia pastoris Secretion Libraries

Posted on Mar 15th, 2017 - By Ulrike Obst, Timothy K. Lu and Volker Sieber

CipA and CipB as Scaffolds To Organize Proteins into Crystalline Inclusions

Posted on Feb 10th, 2017 - By Yang Wang, Ralf Heermann and Kirsten Jung

Engineered Photoactivatable Genetic Switches Based on the Bacterium Phage T7 RNA Polymerase

Posted on Nov 15th, 2016 - By Tiyun Han, Quan Chen and Haiyan Liu

Cyanobacterial Surface Display System Mediates Engineered Interspecies and Abiotic Binding

Posted on Nov 9th, 2016 - By Derek T. Fedeson and Daniel C. Ducat

Reproducibility of High-Throughput Plate-Reader Experiments in Synthetic Biology

Posted on Nov 9th, 2016 - By Michael Chavez, Jonathan Ho and Cheemeng Tan

Fourteen Ways to Reroute Cooperative Communication in the Lactose Repressor: Engineering Regulatory Proteins with Alternate Repressive Functions

Benchmarking of TALE- and CRISPR/dCas9-Based Transcriptional Regulators in Mammalian Cells for the Construction of Synthetic Genetic Circuits

Posted on Jun 28th, 2016 - By Tina Lebar and Roman Jerala

Transcriptomics-Guided Design of Synthetic Promoters for a Mammalian System

Posted on Jun 16th, 2016 - By Joseph K. Cheng and Hal S. Alper

Supervised Learning in Adaptive DNA Strand Displacement Networks

Posted on May 11th, 2016 - By Matthew R. Lakin and Darko Stefanovic

Repurposing Synechocystis PCC6803 UirS–UirR as a UV-Violet/Green Photoreversible Transcriptional Regulatory Tool in E. coli

Posted on May 11th, 2016 - By Prabha Ramakrishnan and Jeffrey J. Tabor

Rewiring Multidomain Protein Switches: Transforming a Fluorescent Zn2+ Sensor into a Light-Responsive Zn2+ Binding Protein

Posted on Apr 12th, 2016 - By Stijn J.A. Aper and Maarten Merkx

Efficient Analysis of Systems Biology Markup Language Models of Cellular Populations Using Arrays

Posted on Mar 8th, 2016 - By Leandro Watanabe and Chris J. Myers

Survival of Phenotypic Information during Cellular Growth Transitions

Posted on Mar 7th, 2016 - By J. Christian J. Ray

Slowing Translation between Protein Domains by Increasing Affinity between mRNAs and the Ribosomal Anti-Shine–Dalgarno Sequence Improves Solubility

Posted on Dec 16th, 2015 - By pthomas2

A Toolbox of Diverse Promoters Related to Methanol Utilization: Functionally Verified Parts for Heterologous Pathway Expression in Pichia pastoris

Posted on Dec 11th, 2015 - By pthomas2

Two Distinct Cyclodipeptide Synthases from a Marine Actinomycete Catalyze Biosynthesis of the Same Diketopiperazine Natural Product

Posted on Dec 9th, 2015 - By pthomas2

In-Yeast Engineering of a Bacterial Genome Using CRISPR/Cas9

Posted on Dec 7th, 2015 - By pthomas2

In Vivo Real-Time Control of Gene Expression: A Comparative Analysis of Feedback Control Strategies in Yeast

Posted on Dec 4th, 2015 - By pthomas2

Memory and Combinatorial Logic Based on DNA Inversions: Dynamics and Evolutionary Stability

Posted on Nov 24th, 2015 - By pthomas2

Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia coli

Posted on Nov 20th, 2015 - By pthomas2

Controlling Central Carbon Metabolism for Improved Pathway Yields in Saccharomyces cerevisiae

Posted on Nov 18th, 2015 - By pthomas2

Development of an Unnatural Amino Acid Incorporation System in the Actinobacterial Natural Product Producer Streptomyces venezuelae ATCC 15439

Posted on Nov 18th, 2015 - By pthomas2

Marching along to an Offbeat Drum: Entrainment of Synthetic Gene Oscillators by a Noisy Stimulus

Posted on Nov 12th, 2015 - By pthomas2

Metabolic Engineering toward Sustainable Production of Nylon-6

Posted on Nov 11th, 2015 - By pthomas2

Engineering a Polyketide Synthase for In Vitro Production of Adipic Acid

Posted on Nov 10th, 2015 - By pthomas2

Electrochemical Measurement of the β-Galactosidase Reporter from Live Cells: A Comparison to the Miller Assay

Posted on Nov 6th, 2015 - By pthomas2

Genome-Wide Tuning of Protein Expression Levels to Rapidly Engineer Microbial Traits

Posted on Nov 5th, 2015 - By pthomas2

CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology

Posted on Nov 4th, 2015 - By pthomas2

Optical Control of Peroxisomal Trafficking

Posted on Nov 2nd, 2015 - By pthomas2

Correlating in Vitro and in Vivo Activities of Light-Inducible Dimers: A Cellular Optogenetics Guide

Posted on Oct 30th, 2015 - By pthomas2

Targeted Large-Scale Deletion of Bacterial Genomes Using CRISPR-Nickases

Posted on Oct 25th, 2015 - By Kylie Standage-Beier, Qi Zhang and Xiao Wang

De Novo Biosynthesis of β-Valienamine in Engineered Streptomyces hygroscopicus 5008

Posted on Oct 13th, 2015 - By pthomas2

Engineering Translational Activators with CRISPR-Cas System

Posted on Oct 8th, 2015 - By pthomas2

Automatic Control of Gene Expression in Mammalian Cells

Posted on Oct 6th, 2015 - By pthomas2

Orthogonal Modular Gene Repression in Escherichia coli Using Engineered CRISPR/Cas9

Posted on Sep 30th, 2015 - By pthomas2

Comprehensive Sequence-Flux Mapping of a Levoglucosan Utilization Pathway in E. coli

Posted on Sep 22nd, 2015 - By pthomas2

A Microfluidic Platform for Long-Term Monitoring of Algae in a Dynamic Environment

Posted on Sep 14th, 2015 - By pthomas2

Regulatable and Modulable Background Expression Control in Prokaryotic Synthetic Circuits by Auxiliary Repressor Binding Sites

Posted on Sep 14th, 2015 - By Davide Merulla and Jan Roelof van der Meer

TALENs-Assisted Multiplex Editing for Accelerated Genome Evolution To Improve Yeast Phenotypes

Posted on Sep 11th, 2015 - By pthomas2

Flexible and Versatile Strategy for the Construction of Large Biochemical Pathways

Posted on Sep 9th, 2015 - By Yongbo Yuan, Erik Andersen and Huimin Zhao

Engineering a Lysine-ON Riboswitch for Metabolic Control of Lysine Production in Corynebacterium glutamicum

Posted on Sep 1st, 2015 - By Li-Bang Zhou and An-Ping Zeng

Engineering Synthetic cis-Regulatory Elements for Simultaneous Recognition of Three Transcriptional Factors in Bacteria

Posted on Aug 31st, 2015 - By pthomas2

Accurate DNA Assembly and Genome Engineering with Optimized Uracil Excision Cloning

Posted on Aug 25th, 2015 - By pthomas2

Evolutionary Design of Choline-Inducible and -Repressible T7-Based Induction Systems

Posted on Aug 25th, 2015 - By pthomas2

Engineering Transcriptional Regulator Effector Specificity Using Computational Design and In Vitro Rapid Prototyping: Developing a Vanillin Sensor

Posted on Aug 19th, 2015 - By pthomas2

Repurposing a Prokaryotic Toxin-Antitoxin System for the Selective Killing of Oncogenically Stressed Human Cells

Posted on Aug 10th, 2015 - By pthomas2

Refactoring the Six-Gene Photosystem II Core in the Chloroplast of the Green Algae Chlamydomonas reinhardtii

Posted on Aug 7th, 2015 - By pthomas2

Synthetic Quorum Sensing and Cell–Cell Communication in Gram-Positive Bacillus megaterium

Posted on Aug 6th, 2015 - By Nicholas Marchand and Cynthia H. Collins

Tunable Riboregulator Switches for Post-transcriptional Control of Gene Expression

Posted on Jul 27th, 2015 - By pthomas2

Probabilistic Analysis of Localized DNA Hybridization Circuits

Posted on Jul 27th, 2015 - By pthomas2

Pterin-Dependent Mono-oxidation for the Microbial Synthesis of a Modified Monoterpene Indole Alkaloid

Posted on Jul 27th, 2015 - By A. M. Ehrenworth, S. Sarria and P. Peralta-Yahya

Engineering of a Synthetic Metabolic Pathway for the Assimilation of (d)-Xylose into Value-Added Chemicals

Posted on Jul 23rd, 2015 - By pthomas2

Tn7-Based Device for Calibrated Heterologous Gene Expression in Pseudomonas putida

Posted on Jul 20th, 2015 - By pthomas2

Development of a Synthetic Malonyl-CoA Sensor in Saccharomyces cerevisiae for Intracellular Metabolite Monitoring and Genetic Screening

Posted on Jul 15th, 2015 - By Sijin Li, Tong Si, Meng Wang and Huimin Zhao

A Barcoding Strategy Enabling Higher-Throughput Library Screening by Microscopy

Posted on Jul 15th, 2015 - By pthomas2

Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide

Posted on Jul 13th, 2015 - By pthomas2

Cell–Cell Communication in Yeast Using Auxin Biosynthesis and Auxin Responsive CRISPR Transcription Factors

Posted on Jul 6th, 2015 - By pthomas2

Genome Calligrapher: A Web Tool for Refactoring Bacterial Genome Sequences for de Novo DNA Synthesis

Generating Effective Models and Parameters for RNA Genetic Circuits

Polymerase Chain Reaction on a Viral Nanoparticle

Posted on Jul 1st, 2015 - By pthomas2

Predicting the Genetic Stability of Engineered DNA Sequences with the EFM Calculator

Posted on Jul 1st, 2015 - By pthomas2

A Versatile Microfluidic Device for Automating Synthetic Biology

Posted on Jun 15th, 2015 - By pthomas2

Synthetic Auxotrophs with Ligand-Dependent Essential Genes for a BL21(DE3) Biosafety Strain

Posted on Jun 15th, 2015 - By Gabriel Lopez and J. Christopher Anderson

Engineering the Controlled Assembly of Filamentous Injectisomes in E. coli K-12 for Protein Translocation into Mammalian Cells

Posted on Jun 12th, 2015 - By pthomas2