A Secretion-Amplification Role for Salmonella enterica Translocon Protein SipD

A Modular Toolkit for Generating Pichia pastoris Secretion Libraries

Posted on Mar 15th, 2017 - By Ulrike Obst, Timothy K. Lu and Volker Sieber

CipA and CipB as Scaffolds To Organize Proteins into Crystalline Inclusions

Posted on Feb 10th, 2017 - By Yang Wang, Ralf Heermann and Kirsten Jung

Engineered Photoactivatable Genetic Switches Based on the Bacterium Phage T7 RNA Polymerase

Posted on Nov 15th, 2016 - By Tiyun Han, Quan Chen and Haiyan Liu

Cyanobacterial Surface Display System Mediates Engineered Interspecies and Abiotic Binding

Posted on Nov 9th, 2016 - By Derek T. Fedeson and Daniel C. Ducat

Reproducibility of High-Throughput Plate-Reader Experiments in Synthetic Biology

Posted on Nov 9th, 2016 - By Michael Chavez, Jonathan Ho and Cheemeng Tan

Fourteen Ways to Reroute Cooperative Communication in the Lactose Repressor: Engineering Regulatory Proteins with Alternate Repressive Functions

Benchmarking of TALE- and CRISPR/dCas9-Based Transcriptional Regulators in Mammalian Cells for the Construction of Synthetic Genetic Circuits

Posted on Jun 28th, 2016 - By Tina Lebar and Roman Jerala

Transcriptomics-Guided Design of Synthetic Promoters for a Mammalian System

Posted on Jun 16th, 2016 - By Joseph K. Cheng and Hal S. Alper

Supervised Learning in Adaptive DNA Strand Displacement Networks

Posted on May 11th, 2016 - By Matthew R. Lakin and Darko Stefanovic

Repurposing Synechocystis PCC6803 UirS–UirR as a UV-Violet/Green Photoreversible Transcriptional Regulatory Tool in E. coli

Posted on May 11th, 2016 - By Prabha Ramakrishnan and Jeffrey J. Tabor

Rewiring Multidomain Protein Switches: Transforming a Fluorescent Zn2+ Sensor into a Light-Responsive Zn2+ Binding Protein

Posted on Apr 12th, 2016 - By Stijn J.A. Aper and Maarten Merkx

Efficient Analysis of Systems Biology Markup Language Models of Cellular Populations Using Arrays

Posted on Mar 8th, 2016 - By Leandro Watanabe and Chris J. Myers

Survival of Phenotypic Information during Cellular Growth Transitions

Posted on Mar 7th, 2016 - By J. Christian J. Ray

Slowing Translation between Protein Domains by Increasing Affinity between mRNAs and the Ribosomal Anti-Shine–Dalgarno Sequence Improves Solubility

Posted on Dec 16th, 2015 - By pthomas2

A Toolbox of Diverse Promoters Related to Methanol Utilization: Functionally Verified Parts for Heterologous Pathway Expression in Pichia pastoris

Posted on Dec 11th, 2015 - By pthomas2

Two Distinct Cyclodipeptide Synthases from a Marine Actinomycete Catalyze Biosynthesis of the Same Diketopiperazine Natural Product

Posted on Dec 9th, 2015 - By pthomas2

In-Yeast Engineering of a Bacterial Genome Using CRISPR/Cas9

Posted on Dec 7th, 2015 - By pthomas2

In Vivo Real-Time Control of Gene Expression: A Comparative Analysis of Feedback Control Strategies in Yeast

Posted on Dec 4th, 2015 - By pthomas2

Memory and Combinatorial Logic Based on DNA Inversions: Dynamics and Evolutionary Stability

Posted on Nov 24th, 2015 - By pthomas2

Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia coli

Posted on Nov 20th, 2015 - By pthomas2

Controlling Central Carbon Metabolism for Improved Pathway Yields in Saccharomyces cerevisiae

Posted on Nov 18th, 2015 - By pthomas2

Development of an Unnatural Amino Acid Incorporation System in the Actinobacterial Natural Product Producer Streptomyces venezuelae ATCC 15439

Posted on Nov 18th, 2015 - By pthomas2

Marching along to an Offbeat Drum: Entrainment of Synthetic Gene Oscillators by a Noisy Stimulus

Posted on Nov 12th, 2015 - By pthomas2

Metabolic Engineering toward Sustainable Production of Nylon-6

Posted on Nov 11th, 2015 - By pthomas2

Engineering a Polyketide Synthase for In Vitro Production of Adipic Acid

Posted on Nov 10th, 2015 - By pthomas2

Electrochemical Measurement of the β-Galactosidase Reporter from Live Cells: A Comparison to the Miller Assay

Posted on Nov 6th, 2015 - By pthomas2

Genome-Wide Tuning of Protein Expression Levels to Rapidly Engineer Microbial Traits

Posted on Nov 5th, 2015 - By pthomas2

CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology

Posted on Nov 4th, 2015 - By pthomas2

Optical Control of Peroxisomal Trafficking

Posted on Nov 2nd, 2015 - By pthomas2

Correlating in Vitro and in Vivo Activities of Light-Inducible Dimers: A Cellular Optogenetics Guide

Posted on Oct 30th, 2015 - By pthomas2

Targeted Large-Scale Deletion of Bacterial Genomes Using CRISPR-Nickases

Posted on Oct 25th, 2015 - By Kylie Standage-Beier, Qi Zhang and Xiao Wang

De Novo Biosynthesis of β-Valienamine in Engineered Streptomyces hygroscopicus 5008

Posted on Oct 13th, 2015 - By pthomas2

Engineering Translational Activators with CRISPR-Cas System

Posted on Oct 8th, 2015 - By pthomas2

Automatic Control of Gene Expression in Mammalian Cells

Posted on Oct 6th, 2015 - By pthomas2

Orthogonal Modular Gene Repression in Escherichia coli Using Engineered CRISPR/Cas9

Posted on Sep 30th, 2015 - By pthomas2

Comprehensive Sequence-Flux Mapping of a Levoglucosan Utilization Pathway in E. coli

Posted on Sep 22nd, 2015 - By pthomas2

A Microfluidic Platform for Long-Term Monitoring of Algae in a Dynamic Environment

Posted on Sep 14th, 2015 - By pthomas2

Regulatable and Modulable Background Expression Control in Prokaryotic Synthetic Circuits by Auxiliary Repressor Binding Sites

Posted on Sep 14th, 2015 - By Davide Merulla and Jan Roelof van der Meer

TALENs-Assisted Multiplex Editing for Accelerated Genome Evolution To Improve Yeast Phenotypes

Posted on Sep 11th, 2015 - By pthomas2

Flexible and Versatile Strategy for the Construction of Large Biochemical Pathways

Posted on Sep 9th, 2015 - By Yongbo Yuan, Erik Andersen and Huimin Zhao

Engineering a Lysine-ON Riboswitch for Metabolic Control of Lysine Production in Corynebacterium glutamicum

Posted on Sep 1st, 2015 - By Li-Bang Zhou and An-Ping Zeng

Engineering Synthetic cis-Regulatory Elements for Simultaneous Recognition of Three Transcriptional Factors in Bacteria

Posted on Aug 31st, 2015 - By pthomas2

Accurate DNA Assembly and Genome Engineering with Optimized Uracil Excision Cloning

Posted on Aug 25th, 2015 - By pthomas2

Evolutionary Design of Choline-Inducible and -Repressible T7-Based Induction Systems

Posted on Aug 25th, 2015 - By pthomas2

Engineering Transcriptional Regulator Effector Specificity Using Computational Design and In Vitro Rapid Prototyping: Developing a Vanillin Sensor

Posted on Aug 19th, 2015 - By pthomas2

Repurposing a Prokaryotic Toxin-Antitoxin System for the Selective Killing of Oncogenically Stressed Human Cells

Posted on Aug 10th, 2015 - By pthomas2

Refactoring the Six-Gene Photosystem II Core in the Chloroplast of the Green Algae Chlamydomonas reinhardtii

Posted on Aug 7th, 2015 - By pthomas2

Synthetic Quorum Sensing and Cell–Cell Communication in Gram-Positive Bacillus megaterium

Posted on Aug 6th, 2015 - By Nicholas Marchand and Cynthia H. Collins

Tunable Riboregulator Switches for Post-transcriptional Control of Gene Expression

Posted on Jul 27th, 2015 - By pthomas2

Probabilistic Analysis of Localized DNA Hybridization Circuits

Posted on Jul 27th, 2015 - By pthomas2

Pterin-Dependent Mono-oxidation for the Microbial Synthesis of a Modified Monoterpene Indole Alkaloid

Posted on Jul 27th, 2015 - By A. M. Ehrenworth, S. Sarria and P. Peralta-Yahya

Engineering of a Synthetic Metabolic Pathway for the Assimilation of (d)-Xylose into Value-Added Chemicals

Posted on Jul 23rd, 2015 - By pthomas2

Tn7-Based Device for Calibrated Heterologous Gene Expression in Pseudomonas putida

Posted on Jul 20th, 2015 - By pthomas2

Development of a Synthetic Malonyl-CoA Sensor in Saccharomyces cerevisiae for Intracellular Metabolite Monitoring and Genetic Screening

Posted on Jul 15th, 2015 - By Sijin Li, Tong Si, Meng Wang and Huimin Zhao

A Barcoding Strategy Enabling Higher-Throughput Library Screening by Microscopy

Posted on Jul 15th, 2015 - By pthomas2

Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide

Posted on Jul 13th, 2015 - By pthomas2

Cell–Cell Communication in Yeast Using Auxin Biosynthesis and Auxin Responsive CRISPR Transcription Factors

Posted on Jul 6th, 2015 - By pthomas2

Genome Calligrapher: A Web Tool for Refactoring Bacterial Genome Sequences for de Novo DNA Synthesis

Generating Effective Models and Parameters for RNA Genetic Circuits

Polymerase Chain Reaction on a Viral Nanoparticle

Posted on Jul 1st, 2015 - By pthomas2

Predicting the Genetic Stability of Engineered DNA Sequences with the EFM Calculator

Posted on Jul 1st, 2015 - By pthomas2

A Versatile Microfluidic Device for Automating Synthetic Biology

Posted on Jun 15th, 2015 - By pthomas2

Synthetic Auxotrophs with Ligand-Dependent Essential Genes for a BL21(DE3) Biosafety Strain

Posted on Jun 15th, 2015 - By Gabriel Lopez and J. Christopher Anderson

Engineering the Controlled Assembly of Filamentous Injectisomes in E. coli K-12 for Protein Translocation into Mammalian Cells

Posted on Jun 12th, 2015 - By pthomas2

The Dual Characteristics of Light-Induced Cryptochrome 2, Homo-oligomerization and Heterodimerization, for Optogenetic Manipulation in Mammalian Cells

Posted on Jun 8th, 2015 - By pthomas2

Enzymatic Menthol Production: One-Pot Approach Using Engineered Escherichia coli

Posted on Jun 5th, 2015 - By pthomas2

Genome Engineering in Cyanobacteria: Where We Are and Where We Need To Go

Posted on Jun 5th, 2015 - By pthomas2

Probing Yeast Polarity with Acute, Reversible, Optogenetic Inhibition of Protein Function

Posted on Jun 2nd, 2015 - By Anna Payne-Tobin Jost and Orion D. Weiner

Partitioning Variability of a Compartmentalized In Vitro Transcriptional Thresholding Circuit

Posted on May 22nd, 2015 - By Korbinian Kapsner and Friedrich C. Simmel

In Vitro Selection for Small-Molecule-Triggered Strand Displacement and Riboswitch Activity

Posted on May 22nd, 2015 - By pthomas2

GPCR-Based Chemical Biosensors for Medium-Chain Fatty Acids

Posted on May 20th, 2015 - By pthomas2

Genome Engineering of the 2,3-Butanediol Biosynthetic Pathway for Tight Regulation in Cyanobacteria

Posted on May 19th, 2015 - By Nicole E. Nozzi and Shota Atsumi

Enhanced Protein Production in Escherichia coli by Optimization of Cloning Scars at the Vector–Coding Sequence Junction

Posted on May 15th, 2015 - By pthomas2

Systematic Identification of a Panel of Strong Constitutive Promoters from Streptomyces albus

Posted on May 7th, 2015 - By pthomas2

Low-Cost, High-Throughput Sequencing of DNA Assemblies Using a Highly Multiplexed Nextera Process

Posted on May 6th, 2015 - By pthomas2

A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly

Posted on May 1st, 2015 - By

ANT: Software for Generating and Evaluating Degenerate Codons for Natural and Expanded Genetic Codes

Posted on Apr 30th, 2015 - By Martin K. M. Engqvist and Jens Nielsen

Influence of Electrostatics on Small Molecule Flux through a Protein Nanoreactor

Posted on Apr 28th, 2015 - By

Fast and Accurate Circuit Design Automation through Hierarchical Model Switching

Posted on Apr 28th, 2015 - By Linh Huynh and Ilias Tagkopoulos

A Hybrid of Metabolic Flux Analysis and Bayesian Factor Modeling for Multiomic Temporal Pathway Activation

Posted on Apr 27th, 2015 - By

Bacterial Recombineering: Genome Engineering via Phage-Based Homologous Recombination

Posted on Apr 27th, 2015 - By

Engineering Escherichia coli into a Protein Delivery System for Mammalian Cells

CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR Arrays for dCas9-Mediated Multiplex Transcriptional Repression in E. coli

BASIC: A New Biopart Assembly Standard for Idempotent Cloning Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology

Rationally Designed MicroRNA-Based Genetic Classifiers Target Specific Neurons in the Brain

Generating Systems Biology Markup Language Models from the Synthetic Biology Open Language

CRISPR-Cas9 Based Engineering of Actinomycetal Genomes

Regulation of Bacterial Gene Expression by Protease-Alleviated Spatial Sequestration (PASS)

Red Light-Regulated Reversible Nuclear Localization of Proteins in Mammalian Cells and Zebrafish

CasEMBLR: Cas9-Facilitated Multiloci Genomic Integration of in Vivo Assembled DNA Parts in Saccharomyces cerevisiae

Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units

Quantifying Impact of Chromosome Copy Number on Recombination in Escherichia coli

Posted on Mar 19th, 2015 - By T. Steele Reynolds and Ryan T. Gill

A tetO Toolkit To Alter Expression of Genes in Saccharomyces cerevisiae

Functional Exchangeability of Oxidase and Dehydrogenase Reactions in the Biosynthesis of Hydroxyphenylglycine, a Nonribosomal Peptide Building Block

Short Synthetic Terminators for Improved Heterologous Gene Expression in Yeast

A Competitive Flow Cytometry Screening System for Directed Evolution of Therapeutic Enzyme

Enhancing Bidirectional Electron Transfer of Shewanella oneidensis by a Synthetic Flavin Pathway

DNA-Linked Enzyme-Coupled Assay for Probing Glucosyltransferase Specificity

Orthogonal Fatty Acid Biosynthetic Pathway Improves Fatty Acid Ethyl Ester Production in Saccharomyces cerevisiae