[ASAP] Improving Chromosome Synthesis with a Semiquantitative Phenotypic Assay and Refined Assembly Strategy

Posted on Sep 19th, 2019 - By pthomas2

[ASAP] Low-Burden Biological Feedback Controllers for Near-Perfect Adaptation

Posted on Sep 12th, 2019 - By Harrison Steel* and Antonis Papachristodoulou*

[ASAP] Modular Thermal Control of Protein Dimerization

[ASAP] Recombination-Independent Genome Editing through CRISPR/Cas9-Enhanced TargeTron Delivery

[ASAP] Development of a Vanillate Biosensor for the Vanillin Biosynthesis Pathway in <italic toggle=”yes”>E. coli</italic>

[ASAP] A Model for the Spatiotemporal Design of Gene Regulatory Circuits<xref rid=”notes-00″ ref-type=”statement”>†</xref>

[ASAP] Construction of Genetic Logic Gates Based on the T7 RNA Polymerase Expression System in <italic toggle=”yes”>Rhodococcus opacus</italic> PD630

Posted on Aug 6th, 2019 - By Drew M. DeLorenzo† and Tae Seok Moon*†‡

[ASAP] Genetic Selection for Small Molecule Production in Competitive Microfluidic Droplets

Posted on Aug 4th, 2019 - By pthomas2

[ASAP] Reversing an Extracellular Electron Transfer Pathway for Electrode-Driven Acetoin Reduction

[ASAP] Fluorescence-Activated Droplet Sorting for Single-Cell Directed Evolution

[ASAP] Genetic Compatibility and Extensibility of Orthogonal Replication

Posted on May 21st, 2019 - By Alex A. Javanpour, Chang C. Liu

[ASAP] Elucidating the Determinants of Polymerase Specificity by Microfluidic-Based Deep Mutational Scanning

Posted on May 12th, 2019 - By Ali Nikoomanzar, Derek Vallejo, John C. Chaput

[ASAP] RNA-Catalyzed Polymerization of Deoxyribose, Threose, and Arabinose Nucleic Acids

[ASAP] Synthetic Bistability and Differentiation in Yeast

Posted on Apr 30th, 2019 - By Yaoyu Yang, Jennifer L. Nemhauser, Eric Klavins

[ASAP] Combinatorial Sensor Design in <italic toggle=”yes”>Caulobacter crescentus</italic> for Selective Environmental Uranium Detection

Posted on Apr 2nd, 2019 - By Dan M. Park, Michael J. Taffet

[ASAP] Building an Inducible T7 RNA Polymerase/T7 Promoter Circuit in <italic toggle=”yes”>Synechocystis</italic> sp. PCC6803

Posted on Apr 2nd, 2019 - By Haojie Jin, Peter Lindblad, Devaki Bhaya

[ASAP] Systematic Evaluation of Genetic and Environmental Factors Affecting Performance of Translational Riboswitches

Posted on Apr 1st, 2019 - By R. Kent, N. Dixon

[ASAP] An Accessible Continuous-Culture Turbidostat for Pooled Analysis of Complex Libraries

[ASAP] Modeling Genetic Circuit Behavior in Transiently Transfected Mammalian Cells

Posted on Mar 27th, 2019 - By Junmin Wang, Samuel A. Isaacson, Calin Belta

[ASAP] Enhanced Pyruvate Production in <italic toggle=”yes”>Candida glabrata</italic> by Engineering ATP Futile Cycle System

[ASAP] Structural Characterization of a Synthetic Tandem-Domain Bacterial Microcompartment Shell Protein Capable of Forming Icosahedral Shell Assemblies

[ASAP] Spatiotemporal Gene Repression System in the Heterocyst-Forming Multicellular Cyanobacterium <italic toggle=”yes”>Anabaena</italic> sp. PCC 7120

Posted on Mar 18th, 2019 - By Akiyoshi Higo, Shigeki Ehira

[ASAP] Desymmetrization of Cyclodepsipeptides by Assembly Mode Switching of Iterative Nonribosomal Peptide Synthetases

[ASAP] Measuring Amber Initiator tRNA Orthogonality in a Genomically Recoded Organism

[ASAP] Temporal Pattern Recognition through Analog Molecular Computation

Posted on Mar 13th, 2019 - By Jackson O’Brien, Arvind Murugan

[ASAP] Construction of Incoherent Feedforward Loop Circuits in a Cell-Free System and in Cells

Posted on Mar 6th, 2019 - By Shaobin Guo, Richard M. Murray

[ASAP] Metabolic Engineering of a Homoserine-Derived Non-Natural Pathway for the <italic toggle=”yes”>De Novo</italic> Production of 1,3-Propanediol from Glucose

[ASAP] Synthetic Inducible Regulatory Systems Optimized for the Modulation of Secondary Metabolite Production in <italic toggle=”yes”>Streptomyces</italic>

Posted on Mar 4th, 2019 - By Chang-Hun Ji, Hiyoung Kim, Hahk-Soo Kang

[ASAP] Physical Plasma Membrane Perturbation Using Subcellular Optogenetics Drives Integrin-Activated Cell Migration

Posted on Feb 22nd, 2019 - By Xenia Meshik, Patrick R. O’Neill, N. Gautam

[ASAP] Combining 26s rDNA and the Cre-loxP System for Iterative Gene Integration and Efficient Marker Curation in <italic toggle=”yes”>Yarrowia lipolytica</italic>

[ASAP] Redirecting Metabolic Flux <italic toggle=”yes”>via</italic> Combinatorial Multiplex CRISPRi-Mediated Repression for Isopentenol Production in <italic toggle=”yes”>Escherichia coli</italic>

[ASAP] Antisense RNA-Based Strategy for Enhancing Surfactin Production in <italic toggle=”yes”>Bacillus subtilis</italic> TS1726 via Overexpression of the Unconventional Biotin Carboxylase II To Enhance ACCase Activity

Posted on Jan 31st, 2019 - By Miaomiao Wang, Huimin Yu, Zhongyao Shen

[ASAP] Engineering a New Class of Anti-LacI Transcription Factors with Alternate DNA Recognition

Posted on Jan 20th, 2019 - By Ronald E. Rondon, Corey J. Wilson

[ASAP] Synthetic Transcription Factors Switch from Local to Long-Range Control during Cell Differentiation

Posted on Jan 17th, 2019 - By Takeo Wada, Sandrine Wallerich, Attila Becskei

[ASAP] An Artificial Pathway for Isoprenoid Biosynthesis Decoupled from Native Hemiterpene Metabolism

Posted on Jan 16th, 2019 - By Sean Lund, Rachael Hall, Gavin J Williams

[ASAP] Metabolic Engineering of <italic toggle=”yes”>Saccharomyces cerevisiae</italic> for Production of Shinorine, a Sunscreen Material, from Xylose

[ASAP] Biological Engineered Living Materials: Growing Functional Materials with Genetically Programmable Properties

Posted on Jan 9th, 2019 - By Charlie Gilbert, Tom Ellis

[ASAP] Structure-Based Design of Versatile Biosensors for Small Molecules Based on the PAS Domain of a Thermophilic Histidine Kinase

Posted on Dec 10th, 2018 - By Kai U. Cormann, Meike Baumgart, Michael Bott

[ASAP] Retroelement-Based Genome Editing and Evolution

[ASAP] Transcription Driven by Reversible Photocontrol of Hyperstable G-Quadruplexes

Posted on Oct 16th, 2018 - By Shinzi Ogasawara

[ASAP] Enhancing Light-Driven 1,3-Propanediol Production by Using Natural Compartmentalization of Differentiated Cells

Posted on Sep 19th, 2018 - By Hongyu Liu, Jun Ni, Ping Xu, Fei Tao

[ASAP] Optimization of Artificial Curcumin Biosynthesis in <italic toggle=”yes”>E. coli</italic> by Randomized 5′-UTR Sequences To Control the Multienzyme Pathway

Posted on Sep 3rd, 2018 - By Sun-Young Kang, Kyung Taek Heo, Young-Soo Hong

[ASAP] Tamoxifen- and Mifepristone-Inducible Versions of CRISPR Effectors, Cas9 and Cpf1

Posted on Sep 3rd, 2018 - By Alazne Dominguez-Monedero, Jamie A. Davies

[ASAP] <italic toggle=”yes”>In Vitro</italic> Transcription Networks Based on Hairpin Promoter Switches

Posted on Jul 31st, 2018 - By Shaunak Kar, Andrew D. Ellington

[ASAP] Robustness of a Reconstituted <italic toggle=”yes”>Escherichia coli</italic> Protein Translation System Analyzed by Computational Modeling

[ASAP] Tight Translational Control Using Site-Specific Unnatural Amino Acid Incorporation with Positive Feedback Gene Circuits

Posted on Jul 19th, 2018 - By Yusuke Kato

[ASAP] Drug-Inducible Control of Lethality Genes: A Low Background Destabilizing Domain Architecture Applied to the Gal4-UAS System in <italic toggle=”yes”>Drosophila</italic>

Posted on May 14th, 2018 - By Manjunatha Kogenaru, Mark Isalan

[ASAP] A Dynamic Model of Resource Allocation in Response to the Presence of a Synthetic Construct

[ASAP] Modularization and Response Curve Engineering of a Naringenin-Responsive Transcriptional Biosensor

Wicket: A Versatile Tool for the Integration and Optimization of Exogenous Pathways in Saccharomyces cerevisiae

Posted on Feb 28th, 2018 - By Sha Hou, Qin Qin and Junbiao Dai

Directed Evolution of Membrane Transport Using Synthetic Selections

Orthogonal Information Encoding in Living Cells with High Error-Tolerance, Safety, and Fidelity

Posted on Feb 21st, 2018 - By Lifu Song and An-Ping Zeng

Genetically Encoded Circuit for Remote Regulation of Cell Migration by Magnetic Fields

Posted on Jan 26th, 2018 - By Abdullah A. Mosabbir and Kevin Truong

Stringency of Synthetic Promoter Sequences in Clostridium Revealed and Circumvented by Tuning Promoter Library Mutation Rates

Posted on Jan 26th, 2018 - By Paweł M. Mordaka and John T. Heap

Analyzing and Tuning Ribozyme Activity by Deep Sequencing To Modulate Gene Expression Level in Mammalian Cells

Posted on Jan 24th, 2018 - By Shungo Kobori and Yohei Yokobayashi

MinGenome: An In Silico Top-Down Approach for the Synthesis of Minimized Genomes

Posted on Jan 20th, 2018 - By Lin Wang and Costas D. Maranas

Metabolic Feedback Circuits Provide Rapid Control of Metabolite Dynamics

Posted on Jan 8th, 2018 - By Di Liu and Fuzhong Zhang

RNA Thermometers for the PURExpress System

Development of a Pigment-Based Whole-Cell Zinc Biosensor for Human Serum

Posted on Dec 22nd, 2017 - By Daniel M. Watstein and Mark P. Styczynski

A Genetic Toolbox for Modulating the Expression of Heterologous Genes in the Cyanobacterium Synechocystis sp. PCC 6803

Cell-Free and In Vivo Characterization of Lux, Las, and Rpa Quorum Activation Systems in E. coli

Posted on Nov 28th, 2017 - By Andrew D. Halleran and Richard M. Murray

Yield Improvement of the Anti-MRSA Antibiotics WAP-8294A by CRISPR/dCas9 Combined with Refactoring Self-Protection Genes in Lysobacter enzymogenes OH11

Controlling Bdellovibrio bacteriovorus Gene Expression and Predation Using Synthetic Riboswitches

Posted on Aug 18th, 2017 - By Mohammed Dwidar and Yohei Yokobayashi

Enabling Graded and Large-Scale Multiplex of Desired Genes Using a Dual-Mode dCas9 Activator in Saccharomyces cerevisiae

Posted on Jul 27th, 2017 - By Matthew Deaner, Julio Mejia and Hal S. Alper

Unique Thermal Stability of Unnatural Hydrophobic Ds Bases in Double-Stranded DNAs

Posted on Jul 26th, 2017 - By Michiko Kimoto and Ichiro Hirao

Developing a Genetically Encoded, Cross-Species Biosensor for Detecting Ammonium and Regulating Biosynthesis of Cyanophycin

Posted on Jul 13th, 2017 - By Yi Xiao, Wen Jiang and Fuzhong Zhang

CAGE: Chromatin Analogous Gene Expression

Posted on Jun 30th, 2017 - By Reza M. Zadegan and William L. Hughes

De Novo Biosynthesis of Glutarate via α-Keto Acid Carbon Chain Extension and Decarboxylation Pathway in Escherichia coli

Autonomous Cell Migration to CSF1 Sources via a Synthetic Protein-Based System

Posted on May 10th, 2017 - By Anam Qudrat and Kevin Truong

Deep Sequencing Analysis of Aptazyme Variants Based on a Pistol Ribozyme

Measurement of In Vivo Protein Binding Affinities in a Signaling Network with Mass Spectrometry

A Glucose-Sensing Toggle Switch for Autonomous, High Productivity Genetic Control

A Secretion-Amplification Role for Salmonella enterica Translocon Protein SipD

A Modular Toolkit for Generating Pichia pastoris Secretion Libraries

Posted on Mar 15th, 2017 - By Ulrike Obst, Timothy K. Lu and Volker Sieber

CipA and CipB as Scaffolds To Organize Proteins into Crystalline Inclusions

Posted on Feb 10th, 2017 - By Yang Wang, Ralf Heermann and Kirsten Jung

Engineered Photoactivatable Genetic Switches Based on the Bacterium Phage T7 RNA Polymerase

Posted on Nov 15th, 2016 - By Tiyun Han, Quan Chen and Haiyan Liu

Cyanobacterial Surface Display System Mediates Engineered Interspecies and Abiotic Binding

Posted on Nov 9th, 2016 - By Derek T. Fedeson and Daniel C. Ducat

Reproducibility of High-Throughput Plate-Reader Experiments in Synthetic Biology

Posted on Nov 9th, 2016 - By Michael Chavez, Jonathan Ho and Cheemeng Tan

Fourteen Ways to Reroute Cooperative Communication in the Lactose Repressor: Engineering Regulatory Proteins with Alternate Repressive Functions

Benchmarking of TALE- and CRISPR/dCas9-Based Transcriptional Regulators in Mammalian Cells for the Construction of Synthetic Genetic Circuits

Posted on Jun 28th, 2016 - By Tina Lebar and Roman Jerala

Transcriptomics-Guided Design of Synthetic Promoters for a Mammalian System

Posted on Jun 16th, 2016 - By Joseph K. Cheng and Hal S. Alper

Supervised Learning in Adaptive DNA Strand Displacement Networks

Posted on May 11th, 2016 - By Matthew R. Lakin and Darko Stefanovic

Repurposing Synechocystis PCC6803 UirS–UirR as a UV-Violet/Green Photoreversible Transcriptional Regulatory Tool in E. coli

Posted on May 11th, 2016 - By Prabha Ramakrishnan and Jeffrey J. Tabor

Rewiring Multidomain Protein Switches: Transforming a Fluorescent Zn2+ Sensor into a Light-Responsive Zn2+ Binding Protein

Posted on Apr 12th, 2016 - By Stijn J.A. Aper and Maarten Merkx

Efficient Analysis of Systems Biology Markup Language Models of Cellular Populations Using Arrays

Posted on Mar 8th, 2016 - By Leandro Watanabe and Chris J. Myers

Survival of Phenotypic Information during Cellular Growth Transitions

Posted on Mar 7th, 2016 - By J. Christian J. Ray

Slowing Translation between Protein Domains by Increasing Affinity between mRNAs and the Ribosomal Anti-Shine–Dalgarno Sequence Improves Solubility

Posted on Dec 16th, 2015 - By pthomas2

A Toolbox of Diverse Promoters Related to Methanol Utilization: Functionally Verified Parts for Heterologous Pathway Expression in Pichia pastoris

Posted on Dec 11th, 2015 - By pthomas2

Two Distinct Cyclodipeptide Synthases from a Marine Actinomycete Catalyze Biosynthesis of the Same Diketopiperazine Natural Product

Posted on Dec 9th, 2015 - By pthomas2

In-Yeast Engineering of a Bacterial Genome Using CRISPR/Cas9

Posted on Dec 7th, 2015 - By pthomas2

In Vivo Real-Time Control of Gene Expression: A Comparative Analysis of Feedback Control Strategies in Yeast

Posted on Dec 4th, 2015 - By pthomas2

Memory and Combinatorial Logic Based on DNA Inversions: Dynamics and Evolutionary Stability

Posted on Nov 24th, 2015 - By pthomas2

Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia coli

Posted on Nov 20th, 2015 - By pthomas2

Controlling Central Carbon Metabolism for Improved Pathway Yields in Saccharomyces cerevisiae

Posted on Nov 18th, 2015 - By pthomas2

Development of an Unnatural Amino Acid Incorporation System in the Actinobacterial Natural Product Producer Streptomyces venezuelae ATCC 15439

Posted on Nov 18th, 2015 - By pthomas2

Marching along to an Offbeat Drum: Entrainment of Synthetic Gene Oscillators by a Noisy Stimulus

Posted on Nov 12th, 2015 - By pthomas2

Metabolic Engineering toward Sustainable Production of Nylon-6

Posted on Nov 11th, 2015 - By pthomas2

Engineering a Polyketide Synthase for In Vitro Production of Adipic Acid

Posted on Nov 10th, 2015 - By pthomas2

Electrochemical Measurement of the β-Galactosidase Reporter from Live Cells: A Comparison to the Miller Assay

Posted on Nov 6th, 2015 - By pthomas2

Genome-Wide Tuning of Protein Expression Levels to Rapidly Engineer Microbial Traits

Posted on Nov 5th, 2015 - By pthomas2