Enabling Graded and Large-Scale Multiplex of Desired Genes Using a Dual-Mode dCas9 Activator in Saccharomyces cerevisiae

Posted on Jul 27th, 2017 - By Matthew Deaner, Julio Mejia and Hal S. Alper

Unique Thermal Stability of Unnatural Hydrophobic Ds Bases in Double-Stranded DNAs

Posted on Jul 26th, 2017 - By Michiko Kimoto and Ichiro Hirao

Developing a Genetically Encoded, Cross-Species Biosensor for Detecting Ammonium and Regulating Biosynthesis of Cyanophycin

Posted on Jul 13th, 2017 - By Yi Xiao, Wen Jiang and Fuzhong Zhang

CAGE: Chromatin Analogous Gene Expression

Posted on Jun 30th, 2017 - By Reza M. Zadegan and William L. Hughes

De Novo Biosynthesis of Glutarate via α-Keto Acid Carbon Chain Extension and Decarboxylation Pathway in Escherichia coli

Autonomous Cell Migration to CSF1 Sources via a Synthetic Protein-Based System

Posted on May 10th, 2017 - By Anam Qudrat and Kevin Truong

Deep Sequencing Analysis of Aptazyme Variants Based on a Pistol Ribozyme

Measurement of In Vivo Protein Binding Affinities in a Signaling Network with Mass Spectrometry

A Glucose-Sensing Toggle Switch for Autonomous, High Productivity Genetic Control

A Secretion-Amplification Role for Salmonella enterica Translocon Protein SipD

A Modular Toolkit for Generating Pichia pastoris Secretion Libraries

Posted on Mar 15th, 2017 - By Ulrike Obst, Timothy K. Lu and Volker Sieber

CipA and CipB as Scaffolds To Organize Proteins into Crystalline Inclusions

Posted on Feb 10th, 2017 - By Yang Wang, Ralf Heermann and Kirsten Jung

Engineered Photoactivatable Genetic Switches Based on the Bacterium Phage T7 RNA Polymerase

Posted on Nov 15th, 2016 - By Tiyun Han, Quan Chen and Haiyan Liu

Cyanobacterial Surface Display System Mediates Engineered Interspecies and Abiotic Binding

Posted on Nov 9th, 2016 - By Derek T. Fedeson and Daniel C. Ducat

Reproducibility of High-Throughput Plate-Reader Experiments in Synthetic Biology

Posted on Nov 9th, 2016 - By Michael Chavez, Jonathan Ho and Cheemeng Tan

Fourteen Ways to Reroute Cooperative Communication in the Lactose Repressor: Engineering Regulatory Proteins with Alternate Repressive Functions

Benchmarking of TALE- and CRISPR/dCas9-Based Transcriptional Regulators in Mammalian Cells for the Construction of Synthetic Genetic Circuits

Posted on Jun 28th, 2016 - By Tina Lebar and Roman Jerala

Transcriptomics-Guided Design of Synthetic Promoters for a Mammalian System

Posted on Jun 16th, 2016 - By Joseph K. Cheng and Hal S. Alper

Supervised Learning in Adaptive DNA Strand Displacement Networks

Posted on May 11th, 2016 - By Matthew R. Lakin and Darko Stefanovic

Repurposing Synechocystis PCC6803 UirS–UirR as a UV-Violet/Green Photoreversible Transcriptional Regulatory Tool in E. coli

Posted on May 11th, 2016 - By Prabha Ramakrishnan and Jeffrey J. Tabor

Rewiring Multidomain Protein Switches: Transforming a Fluorescent Zn2+ Sensor into a Light-Responsive Zn2+ Binding Protein

Posted on Apr 12th, 2016 - By Stijn J.A. Aper and Maarten Merkx

Efficient Analysis of Systems Biology Markup Language Models of Cellular Populations Using Arrays

Posted on Mar 8th, 2016 - By Leandro Watanabe and Chris J. Myers

Survival of Phenotypic Information during Cellular Growth Transitions

Posted on Mar 7th, 2016 - By J. Christian J. Ray

Slowing Translation between Protein Domains by Increasing Affinity between mRNAs and the Ribosomal Anti-Shine–Dalgarno Sequence Improves Solubility

Posted on Dec 16th, 2015 - By pthomas2

A Toolbox of Diverse Promoters Related to Methanol Utilization: Functionally Verified Parts for Heterologous Pathway Expression in Pichia pastoris

Posted on Dec 11th, 2015 - By pthomas2

Two Distinct Cyclodipeptide Synthases from a Marine Actinomycete Catalyze Biosynthesis of the Same Diketopiperazine Natural Product

Posted on Dec 9th, 2015 - By pthomas2

In-Yeast Engineering of a Bacterial Genome Using CRISPR/Cas9

Posted on Dec 7th, 2015 - By pthomas2

In Vivo Real-Time Control of Gene Expression: A Comparative Analysis of Feedback Control Strategies in Yeast

Posted on Dec 4th, 2015 - By pthomas2

Memory and Combinatorial Logic Based on DNA Inversions: Dynamics and Evolutionary Stability

Posted on Nov 24th, 2015 - By pthomas2

Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia coli

Posted on Nov 20th, 2015 - By pthomas2

Controlling Central Carbon Metabolism for Improved Pathway Yields in Saccharomyces cerevisiae

Posted on Nov 18th, 2015 - By pthomas2

Development of an Unnatural Amino Acid Incorporation System in the Actinobacterial Natural Product Producer Streptomyces venezuelae ATCC 15439

Posted on Nov 18th, 2015 - By pthomas2

Marching along to an Offbeat Drum: Entrainment of Synthetic Gene Oscillators by a Noisy Stimulus

Posted on Nov 12th, 2015 - By pthomas2

Metabolic Engineering toward Sustainable Production of Nylon-6

Posted on Nov 11th, 2015 - By pthomas2

Engineering a Polyketide Synthase for In Vitro Production of Adipic Acid

Posted on Nov 10th, 2015 - By pthomas2

Electrochemical Measurement of the β-Galactosidase Reporter from Live Cells: A Comparison to the Miller Assay

Posted on Nov 6th, 2015 - By pthomas2

Genome-Wide Tuning of Protein Expression Levels to Rapidly Engineer Microbial Traits

Posted on Nov 5th, 2015 - By pthomas2

CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology

Posted on Nov 4th, 2015 - By pthomas2

Optical Control of Peroxisomal Trafficking

Posted on Nov 2nd, 2015 - By pthomas2

Correlating in Vitro and in Vivo Activities of Light-Inducible Dimers: A Cellular Optogenetics Guide

Posted on Oct 30th, 2015 - By pthomas2

Targeted Large-Scale Deletion of Bacterial Genomes Using CRISPR-Nickases

Posted on Oct 25th, 2015 - By Kylie Standage-Beier, Qi Zhang and Xiao Wang

De Novo Biosynthesis of β-Valienamine in Engineered Streptomyces hygroscopicus 5008

Posted on Oct 13th, 2015 - By pthomas2

Engineering Translational Activators with CRISPR-Cas System

Posted on Oct 8th, 2015 - By pthomas2

Automatic Control of Gene Expression in Mammalian Cells

Posted on Oct 6th, 2015 - By pthomas2

Orthogonal Modular Gene Repression in Escherichia coli Using Engineered CRISPR/Cas9

Posted on Sep 30th, 2015 - By pthomas2

Comprehensive Sequence-Flux Mapping of a Levoglucosan Utilization Pathway in E. coli

Posted on Sep 22nd, 2015 - By pthomas2

A Microfluidic Platform for Long-Term Monitoring of Algae in a Dynamic Environment

Posted on Sep 14th, 2015 - By pthomas2

Regulatable and Modulable Background Expression Control in Prokaryotic Synthetic Circuits by Auxiliary Repressor Binding Sites

Posted on Sep 14th, 2015 - By Davide Merulla and Jan Roelof van der Meer

TALENs-Assisted Multiplex Editing for Accelerated Genome Evolution To Improve Yeast Phenotypes

Posted on Sep 11th, 2015 - By pthomas2

Flexible and Versatile Strategy for the Construction of Large Biochemical Pathways

Posted on Sep 9th, 2015 - By Yongbo Yuan, Erik Andersen and Huimin Zhao

Engineering a Lysine-ON Riboswitch for Metabolic Control of Lysine Production in Corynebacterium glutamicum

Posted on Sep 1st, 2015 - By Li-Bang Zhou and An-Ping Zeng

Engineering Synthetic cis-Regulatory Elements for Simultaneous Recognition of Three Transcriptional Factors in Bacteria

Posted on Aug 31st, 2015 - By pthomas2

Accurate DNA Assembly and Genome Engineering with Optimized Uracil Excision Cloning

Posted on Aug 25th, 2015 - By pthomas2

Evolutionary Design of Choline-Inducible and -Repressible T7-Based Induction Systems

Posted on Aug 25th, 2015 - By pthomas2

Engineering Transcriptional Regulator Effector Specificity Using Computational Design and In Vitro Rapid Prototyping: Developing a Vanillin Sensor

Posted on Aug 19th, 2015 - By pthomas2

Repurposing a Prokaryotic Toxin-Antitoxin System for the Selective Killing of Oncogenically Stressed Human Cells

Posted on Aug 10th, 2015 - By pthomas2

Refactoring the Six-Gene Photosystem II Core in the Chloroplast of the Green Algae Chlamydomonas reinhardtii

Posted on Aug 7th, 2015 - By pthomas2

Synthetic Quorum Sensing and Cell–Cell Communication in Gram-Positive Bacillus megaterium

Posted on Aug 6th, 2015 - By Nicholas Marchand and Cynthia H. Collins

Tunable Riboregulator Switches for Post-transcriptional Control of Gene Expression

Posted on Jul 27th, 2015 - By pthomas2

Probabilistic Analysis of Localized DNA Hybridization Circuits

Posted on Jul 27th, 2015 - By pthomas2

Pterin-Dependent Mono-oxidation for the Microbial Synthesis of a Modified Monoterpene Indole Alkaloid

Posted on Jul 27th, 2015 - By A. M. Ehrenworth, S. Sarria and P. Peralta-Yahya

Engineering of a Synthetic Metabolic Pathway for the Assimilation of (d)-Xylose into Value-Added Chemicals

Posted on Jul 23rd, 2015 - By pthomas2

Tn7-Based Device for Calibrated Heterologous Gene Expression in Pseudomonas putida

Posted on Jul 20th, 2015 - By pthomas2

Development of a Synthetic Malonyl-CoA Sensor in Saccharomyces cerevisiae for Intracellular Metabolite Monitoring and Genetic Screening

Posted on Jul 15th, 2015 - By Sijin Li, Tong Si, Meng Wang and Huimin Zhao

A Barcoding Strategy Enabling Higher-Throughput Library Screening by Microscopy

Posted on Jul 15th, 2015 - By pthomas2

Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide

Posted on Jul 13th, 2015 - By pthomas2

Cell–Cell Communication in Yeast Using Auxin Biosynthesis and Auxin Responsive CRISPR Transcription Factors

Posted on Jul 6th, 2015 - By pthomas2

Genome Calligrapher: A Web Tool for Refactoring Bacterial Genome Sequences for de Novo DNA Synthesis

Generating Effective Models and Parameters for RNA Genetic Circuits

Polymerase Chain Reaction on a Viral Nanoparticle

Posted on Jul 1st, 2015 - By pthomas2

Predicting the Genetic Stability of Engineered DNA Sequences with the EFM Calculator

Posted on Jul 1st, 2015 - By pthomas2

A Versatile Microfluidic Device for Automating Synthetic Biology

Posted on Jun 15th, 2015 - By pthomas2

Synthetic Auxotrophs with Ligand-Dependent Essential Genes for a BL21(DE3) Biosafety Strain

Posted on Jun 15th, 2015 - By Gabriel Lopez and J. Christopher Anderson

Engineering the Controlled Assembly of Filamentous Injectisomes in E. coli K-12 for Protein Translocation into Mammalian Cells

Posted on Jun 12th, 2015 - By pthomas2

The Dual Characteristics of Light-Induced Cryptochrome 2, Homo-oligomerization and Heterodimerization, for Optogenetic Manipulation in Mammalian Cells

Posted on Jun 8th, 2015 - By pthomas2

Enzymatic Menthol Production: One-Pot Approach Using Engineered Escherichia coli

Posted on Jun 5th, 2015 - By pthomas2

Genome Engineering in Cyanobacteria: Where We Are and Where We Need To Go

Posted on Jun 5th, 2015 - By pthomas2

Probing Yeast Polarity with Acute, Reversible, Optogenetic Inhibition of Protein Function

Posted on Jun 2nd, 2015 - By Anna Payne-Tobin Jost and Orion D. Weiner

Partitioning Variability of a Compartmentalized In Vitro Transcriptional Thresholding Circuit

Posted on May 22nd, 2015 - By Korbinian Kapsner and Friedrich C. Simmel

In Vitro Selection for Small-Molecule-Triggered Strand Displacement and Riboswitch Activity

Posted on May 22nd, 2015 - By pthomas2

GPCR-Based Chemical Biosensors for Medium-Chain Fatty Acids

Posted on May 20th, 2015 - By pthomas2

Genome Engineering of the 2,3-Butanediol Biosynthetic Pathway for Tight Regulation in Cyanobacteria

Posted on May 19th, 2015 - By Nicole E. Nozzi and Shota Atsumi

Enhanced Protein Production in Escherichia coli by Optimization of Cloning Scars at the Vector–Coding Sequence Junction

Posted on May 15th, 2015 - By pthomas2

Systematic Identification of a Panel of Strong Constitutive Promoters from Streptomyces albus

Posted on May 7th, 2015 - By pthomas2

Low-Cost, High-Throughput Sequencing of DNA Assemblies Using a Highly Multiplexed Nextera Process

Posted on May 6th, 2015 - By pthomas2

A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly

Posted on May 1st, 2015 - By

ANT: Software for Generating and Evaluating Degenerate Codons for Natural and Expanded Genetic Codes

Posted on Apr 30th, 2015 - By Martin K. M. Engqvist and Jens Nielsen

Influence of Electrostatics on Small Molecule Flux through a Protein Nanoreactor

Posted on Apr 28th, 2015 - By

Fast and Accurate Circuit Design Automation through Hierarchical Model Switching

Posted on Apr 28th, 2015 - By Linh Huynh and Ilias Tagkopoulos

A Hybrid of Metabolic Flux Analysis and Bayesian Factor Modeling for Multiomic Temporal Pathway Activation

Posted on Apr 27th, 2015 - By

Bacterial Recombineering: Genome Engineering via Phage-Based Homologous Recombination

Posted on Apr 27th, 2015 - By

Engineering Escherichia coli into a Protein Delivery System for Mammalian Cells

CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR Arrays for dCas9-Mediated Multiplex Transcriptional Repression in E. coli

BASIC: A New Biopart Assembly Standard for Idempotent Cloning Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology

Rationally Designed MicroRNA-Based Genetic Classifiers Target Specific Neurons in the Brain

Generating Systems Biology Markup Language Models from the Synthetic Biology Open Language

CRISPR-Cas9 Based Engineering of Actinomycetal Genomes

Regulation of Bacterial Gene Expression by Protease-Alleviated Spatial Sequestration (PASS)

Red Light-Regulated Reversible Nuclear Localization of Proteins in Mammalian Cells and Zebrafish

CasEMBLR: Cas9-Facilitated Multiloci Genomic Integration of in Vivo Assembled DNA Parts in Saccharomyces cerevisiae