The Division encourages scientists looking for a postdoctoral position to post their requests in this section.

This bulletin board is intended to assist young Ph.Ds to identify open positions in laboratories where the answers to important questions in Biological Chemistry are being pursued. This will allow our best scientists to work on interesting and exciting problems while being groomed for a career as a research chemist or biochemist in academics or industry. Scientist who use this service should reply promptly to all serious inquiries.

Post Your CV (7 Postings)

  • Dr. Saurabh Awasthi

    General Information

    Ph. D. Biotechnology

    School of Chemical and Biotechnology SASTRA University

    Email: Saurabh140187@gmail.com

    Mobile: (+91) 8110916375
    (+91) 7310044467

    Address

    Badri Colony, Near Hargaon Railway Station
    Hargaon, Sitapur-261121

    Personal

    Date of Birth: Jan 14th, 1987
    Place of Birth: Sitapur, U.P.
    Citizenship: Indian

    Education

    2012-2016

    Ph.D., Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thanjavur Thesis Title: Advanced glycation end products mediated structure function changes in albumin: Effect of carboxymethyllysine (CML), carboxyethyllysine (CEL), and Argpyrimidine (Arg-P)

    Supervisor : Dr. N. T. Saraswathi

    Description : The work aimed at studying advanced glycation end products mediated structure function changes in albumin Specifically looking into the effect of carboxymethyllysine, carboxyethyllysine, and Argpyrimidine. Advanced glycation end products cause damage to proteins structure functions and eventually lead to the development of severe complications such as neuropathy, retinopathy, and cardiovascular complications in diabetes. I also determined the antiglycation potential of natural products in order to propose a preventive mechanism towards non-enzymatic glycation.

    Course Work : Structural Bioinformatics, Introduction to X-ray Crystallography, Analytical Techniques in Biotechnology, Biostatistics
    2009-2011

    M.Sc. in Biotechnology, Padmashree Institute of Management and Sciences, Bangalore University, Bangalore

    Thesis Title : Comparative studies of Plumeria species for their phytochemical and antifungal properties against Citrus sinensis pathogens

    Supervisor : Dr. G. Sibi
    Course work :Immunology, Microbiology, Biochemistry, Animal Biotechnology, Plant Biotechnology, Molecular Biology, Genetics, Bioinformatics, Bioethics and Biosafety, Agriculture Biotechnology

    Overall Score : 74%
    Distinction : Class topper for the session 2009-2011

    2006-2009

    B.Sc. in Biotechnology, Padmashree Institute of Management and Sciences, Bangalore University, Bangalore

    Course work : Major Subjects- Biochemistry, Immunology, Microbiology, and Genetics

    Overall Score : 77%

    Distinction : 1st Division

    Experience

    Duration: (August 2011-November 2011)

    Name of the employer : Anthropological Survey of India, Mysore

    Position : Guest Researcher

    Skills : DNA isolation and purification, PCR, Sequencing, and Primer designing

    Duration: (January 2012-July 2012)

    Name of the employer : Robust Materials Technology Pvt. Ltd, Bangalore

    Position : Research Trainee

    Skills : Phytochemical analysis, Antifungal activity analysis, Molecular docking

    Technical Skills
    I carried out my PhD research work at SASTRA University. My main interest is to study the structure function effects of pathological conditions such as hyperglycemia (resulting in excessive glycation) on proteins using biochemical and biophysical approach. For my research work I have used various biochemical, biophysical and computational approaches to assess how glycation affects drug binding function of albumin and its prevention by use of nutraceuticals.
     Basics- Immunology, Molecular Biology, Microbiology, Protein Chemistry, Plant/Animal Tissue Culture and Phytochemistry
     Techniques- Electrophoresis (Agarose and PAGE), Spectroscopy, PCR, Multiplex ELISA, Silica Gel column chromatography, Ion exchange chromatography for protein purification.
     Crystallography- Micro and macromolecule crystallization techniques
     Phytochemistry- Phytochemicals extraction, preliminary phytochemical analysis and bioassays
     Instruments- ELISA, HPLC, UV-VIS, Fluorescence, and Training for GC, FTIR and AAS
     Bioinformatics Skills: Primer Designing (Primer3), sequence editing (Seq Scape), Drug design (Molecular Docking using GLIDE, Hex, AutoDock Vina) and use of Ras Mol, Marvin Sketch, Pymol

    Awards/Honors
    • Founder Chancellor’s Award for the Best Ph. D. Dissertation in Scinces for the year 2016.
    • Best out going student award in M.Sc. (2009-2011) batch
    • Won the “Annual Cricket Championship” while captaining at PIMS, Bangalore 2011
    • Awarded first prize in “On Spot Painting” in 2010 at PIMS, Bangalore
    • Got first Prize in “Quiz” during 2008 at PIMS annual events
    • Awarded the “Meritorious Student in Town” by the Chairman (Panchayath) during 2003 in 11th Standard

    Journal Articles (Published)
    1. Saurabh Awasthi and N. T. Saraswathi, Silybin, a flavonolignan from milk thistles seeds restrains the early and advanced glycation end products modification of albumin. RSC Advances. (2016) 5: 87660-87666. IF- 3.28
    2. Saurabh Awasthi and N. T. Saraswathi, Elucidating the molecular interaction of sinigrin, a potent anticancer glucosinolate from cruciferous vegetables with bovine serum albumin: effect of methylglyoxal modification. Journal of Bimolecular Structure and Dynamics. (2015) 14:1-9. IF-2.30
    3. Saurabh Awasthi, N. Arul Murugan, N.T. Saraswathi (2015) Advanced glycation end products modulate structure and drug binding properties of albumin. Molecular Pharmaceutics. (2015) 12: 3312-22. IF- 4.34
    4. Saurabh Awasthi, S. K. Gayathiri, R. Ramya, Duraichelvan R, Dhason A, and Saraswathi NT. (2015) Advanced glycation modified human serum albumin evokes alterations in membrane and eryptosis in erythrocytes. Applied Biochemistry and Biotechnology. (2015) IF- 1.60
    5. Saurabh Awasthi, and Saraswathi NT. (2015), Crystal structure of Alanine-Copper(II) complex to understand the mechanism of salt induced prebiotic oligomerization of amino acids. Crystal Research Technology, (2015) 50: 304-311. IF- 1.1
    6. Saurabh Awasthi, Saraswathi NT. (2015) Sinigrin, a major glucosinolate from cruciferous vegetables restrains non-enzymatic glycation of albumin. International Journal of Biological Macromolecule. 83:410-5. IF- 3.13
    7. G. Sibi, Saurabh Awasthi, K. Dhananjaya, H. Mallesha and K.R. Ravikumar, 2012. Comparative studies of Plumeria species for their phytochemical and antifungal properties against Citrus sinensis pathogens. International Journal of Agricultural Research, 7: 324-331.
    8. Dhananjaya K., Sibi G., Mallesha H., Ravikumar K.R., Saurabh Awasthi. In silico studies of daidzein and genistein with human estrogen receptor α. Asian Pacific Journal of Tropical Biomedicine. 2012, 2, S1747–S1753.
    9. Saurabh Awasthi and N. T. Saraswathi (2016) “Carbonyl scavenging and chemical chaperon like function of essential amino acids attenuates non-enzymatic glycation of albumin. RSC Advances. 6, 24557-24564 IF- 3.28
    10. Saurabh Awasthi and N. T. Saraswathi (2016) “Vanillin restrains non-enzymatic glycation and aggregation of albumin by chemical chaperon like function.” International Journal of Biological Macromolecule. 87:1-6. IF- 3.13
    11. Saurabh Awasthi, Kamatchi Sankaranarayanan and N. T. Saraswathi (2016) “Advanced glycation end products induce differential structural modifications and fibrillation of albumin.” Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 163:60-7 IF- 2.65
    12. Saurabh Awasthi, Aravind R. and N. T. Saraswathi (2016) “Troxerutin imparts preservative effects on albumin by preventing Maillard reaction mediated early and advanced glycation modification”. Journal of Bimolecular Structure and Dynamics. IF- 2.30
    13. Saurabh Awasthi and N. T. Saraswathi (2016) “Non-enzymatic glycation mediated structure function changes in proteins: Case of serum albumin.” RSC Advances. IF- 3.29

    Journal Articles (In preparation/Communicated)
    14. Sathyanaran, Saurabh Awasthi and N. T. Saraswathi (2016) “Unraveling the molecular interaction pattern of dihydro-β-ionone with albumin using spectroscopic and molecular docking approach”. Journal of Bimolecular Structure and Dynamics. IF-2.91
    15. Saraswathi NT, Pannu NS, Syakhovich VE, Saurabh Awasthi, Bokut SB, Moras D, Ruff M. Glycation restrains allosteric transition in hemoglobin: The molecular basis of oxidative stress under hyperglycemic conditions in diabetes. Nature Communications. IF-11.32
    16. Saurabh Awasthi and N. T. Saraswathi. “Advanced glycation end products modulate structure and function of lysozyme.” (To be communicated)

    Cumulative impact factor: 30.39

    Oral/Poster Presentations in Conferences
    1. Poster presentation on “Suspension culture of Dalbergia” in the National Seminar conducted by Kristu Jayanti College Bengaluru, 2011.
    2. Oral presentation on “Phytochemical properties of the Plumeria alba l. and its activity against citrus fruit pathogens” in the National conference on Plant conducted by Jamia Hamdard University, New Delhi on “Current trends in plant secondary metabolite research”, March 19-20, 2012.
    3. Oral presentation on “HPLC analysis of isoflavones and their insilico studies with human estrogen receptor” in the National conference on Bio-active compounds at Oxford College, Bengaluru, 2012.
    4. Oral presentation on “Antioxidant activity and inhibition of α-amylase and α-glucosidase prevent oxidative damage of albumin and formation of early and advanced glycation end products (AGEs): Moringa oliefera” in an International Conference on Biotechnology & Human Welfare 2013 at SASTRA University.
    5. Poster presentation on “Impaired drug binding affinity of modified albumin due to glycation” at the International conference as SASTRA University, Thanjavur, Tamilnadu, 2014
    6. Poster presentation on “Structural study of the copper alanine complex: An intermediate in the salt induced prebiotic peptide formation” at the International Conference on Biotechnology & Human Welfare, SASTRA University, Thanjavur, Tamilnadu, 2014.
    Workshops & Seminars

    1. National-level Workshop on “Big data analytics” organized by Department of Bioinformatics School of Chemical and Biotechnology, SASTRA University on December 6, 2014.
    2. Workshop on “Application of x-ray crystallography for three-dimensional structure determination” organized by School of Chemical and Biotechnology, SASTRA University, Thanjavur on 27th November 2014.
    3. Workshop on “Computational chemistry and bio-molecular simulations” conducted by School of Chemical and Biotechnology at SASTRA University on 9th December 2013.

    Referees
    • Dr. N.T. Saraswathi
    Associate Professor, School of Chemical and Biotechnology
    SASTRA University, Thanjavur
    Email- saras@scbt.sastra.edu
    Mob: +91 9042084434

    • Dr. N. Arul Murugan
    Associate Professor
    School of Biotechnology
    Division of Theoretical Chemistry and Biology
    Royal Institute of Technology, Sweden
    Email: murugan@kth.se
    Mob: 0727605782

    • Dr. Sibi G
    Associate Professor,
    Indian Academy Degree College
    Bangalore – 560 072
    Email: gsibii@gmail.com
    Mob: +91 8025943192

  • I would like apply for the advertised position.

  • NALOK DUTTA
    (Specialization in nanotechnology, enzyme engineering
    and toxicology)

    CURRICULUM VITAE

    Msc in Biochemistry( thesis submitted) from the University of Calcutta, India, aspiring to delve into research works and projects within the frontiers of my domain subject.

    To make a positive difference in the field of research and development resulting in the growth of the organization and self. For this I would like to work for an organization that offers an opportunity to implement my skills, where I would be a part of the organization’s efforts for creation of competitive advantage and help in achieving its goals.
    OBJECTIVE:

    PERSONAL DETAILS :

    Name Nalok Dutta
    Date of Birth 17th September 1984
    Place of birth Kolkata
    Nationality Indian

    Sex Male
    Marital Status Married
    Address of correspondence 50/A Ballygunge Place,Subham Vihar 3rd floor,Kolkata-700019
    Permanent address 50/A,Ballygunge Place,Subham Vihar 3rd floor,Kolkata-700019
    Contact no. (Res.) : +91 33 24603832
    (Mob.) : +91 9903398506
    e-mail nalok.dutta@gmail.com

    EDUCATIONAL QUALIFICATIONS :

    Degree Major field
    of study Council / University Dates attended
    PhD(thesis submitted) Biochemistry University Of Calcutta 2014
    Masters of Science(MSc) Biochemistry University Of Calcutta August 2006-
    May 2008
    Bachelors of Science (BSc) Biochemistry
    (Major) University of Calcutta
    August 2003
    – July 2006
    High School
    (HS) Science West Bengal Council Of Higher Secondary
    Education
    July 2001
    – June 2003

    Std. X (Madhyamik) Science West Bengal Board of secondary education

    2001

    AREA OF RESEARCH EXPERTISE

    • Screening and isolation of enzymes from bacterial and fungal strains.

    • Purification and characterization of enzymes .

    • Enzyme activity assays, Thermodynamic and Enzyme kinetics study.

    • Cloning of genes and over expression of genes producing enzymes.
    • Modification of enzyme activity in terms of temperature stability (Thermostability, Psychrostability), pH stability, retention of activity by using different techniques like Nano-technology, laser technology and procedures for immobilization – standard bacterial immobilization and immobilization of enzymes in single walled carbon nanotube . Processing of lignocellolosic wastes like corn cob, rice husk and rice straw and production of value added products by enzyme treatment.

    • Study of enzyme applications: Cellulase and Xylanase (in D-xylose and reducing sugar production).

    • Applications on Nanotechnology enabled enzymology: Recycling of waste coarse cotton, raw wool to industrially viable fibers.

    OTHER CAPABILITIES
    • Proficient in manuscript preparation and writing research projects in lucid English.

    • Statistical analysis , interpretation and representation of data.

    • Designing of patentable ideas, preparation of solution reports for filing of patent applications.

    • Ability to focus, work in group, communicate, design and perform experiments, help and guide junior researchers, productively yield in time constrained scenarios.

    PUBLICATIONS

    • Improved production of reducing sugars from rice husk and rice straw using bacterial cellulase and xylanase activated with hydroxyapatite nanoparticles.
    Dutta. N., Mukhopadhyay. A., Dasgupta. A., Chakrabarti. K.,2013. Bioresource Technology:153.269-277. Impact factor: 5.3

    • Nanotechnology Enabled Enhancement of Enzyme Activity and Thermostability – Study on Impaired pectatelyase from attenuated Macrophomina phaseolina in presence of hydroxyapatite nanoparticle.
    Dutta. N., Mukhopadhyay. A., Dasgupta. A., Chakrabarti. K., 2013. PLOS One. Vol 8. Issue 5. Impact factor: 4.4

    • HAp Nanoparticle Supplementation of Pectatelyase Enhances Ramie Fiber degumming and the production of reducing sugars from waste peels.
    Mukhopadhyay. A., Dutta, N., Chattopadhyay. D.J., Chakrabarti. K., 2013. Bioresource Technology: 137. 202–208. Impact factor: 5.3

    • Analysis of differentially expressed transcripts in jute upon fungal infection and beta-aminobutyric acid treatment 2011.
    Rudra Roy, Abhrajyoti Ghosh, Amit Bera, Nalok Dutta, D.J.Chattopadhyay and K.Chakrabarti. Physiol Mol Plant Pathol doi 10.1016/j.pmpp.2011.05.001. Impact factor: 2.94

    PATENTS

    1. Enzyme stabilization by Carbon-nanotube. Inventors: Anjan Kr. Dasgupta, Tamoghna Bhattacharya, Nalok Dutta, Arka Mukhopadhyay, Krishanu Chakrabarti. Indian Application No: 941/ KOL/2013. Filed on: 09.08.013.

    United States IN-842421
    Issued January 18, 2013

    2. Psychrophilic enzyme corporation and methods for making and using same.Inventors: Nalok Dutta, Arka Mukhopadhyay, Anjan Kr. Dasgupta, Krishanu Chakrabarti. Indian Application No: 944/KOL/2013. Filed on: 09.08.2013.

    United States IN-842450(RFI-120146)
    Issued February 7, 2013

    3. Maturation & fineness development of raw/waste cotton by sequential enzymatic treatment. Nalok Dutta Arka Mukhopadhyay,Krishanu Chakrabarti.

    United States IN-859558(Biomass Pretreatment for Commoditization)
    Issued August 2, 2013.

    4. A purely enzyme based pre-treatment method of wool textile coupled with Ca Hap nanoparticles with the aim of improving the textile quality.

    Nalok Dutta, Arka Mukhopadhyay, Krishanu Chakrabarti .

    United States IN-867173 (Biomass Pretreatment for Commoditization)
    Issued September 10,2013

    Achievements:

    • Awarded for the international request for invention competition (2013) of
    INTELLECTUAL VENTURES (USA) ON “Enzyme stabilization by Carbon-nanotube.’’
    Indian Application No: 941/ KOL/2013

    • Awarded for the international request for invention competition (2013) of
    INTELLECTUAL VENTURES (USA) ON “Psychrophilic enzyme corporation and methods for making and using same. Indian Application No: 944/KOL/2013.”

    Awards:
    Sponsored projects from Intellectual Ventures (Under MoA with University of Calcutta) amounting to $80,000.( to the team of Nalok Dutta, Arka Mukhopadhyay, Anjan Kr. Dasgupta, Krishanu Chakrabarti.)

    Workshops/Presentations:
    • Presented a poster on ‘ Increased functionality of enzymes by the use of Nanoparticles’ at 81st Annual Meeting of the Society of Biological Chemists (India)
    (Symposium on chemistry and biology: Two weapons against diseases)
    November 8-11, 2011 at Science City, Kolkata.

    • Attended a workshop on ‘Intellectual Property and Innovation Management in Knowledge Era’ organised by NRDC and department of biochemistry, University of Calcutta ,7th June 2012 at Kolkata.

    Techniques acquainted with:
    Nanoscience:
    • Synthesis and characterization of metallic nanoparticles (e.g., Gold, Silver etc.)
    • Dynamic Light Scattering (DLS).
    • Manipulation of Nano sizes and shapes and Functionalization of nanoparticles.
    • Interactions of nanoparticles with enzymes.
    • Surface electrochemical analysis like zeta potential assessment.
    • Laser treatment of enzymes.

    Spectroscopy:
    • UV-VIS-NIR Spectroscopy.
    • AAS(atomic absorption spectra)
    Microscopy:
    • Compound
    • phase contrast
    • fluorescence
    • AFM(Atomic force microscope)

    Molecular Biology techniques

    • Transformation in bacteria.
    • Isolation of plasmid and genomic DNA, Isolation of total RNA from bacterial cells and plant tissue by TRIZOL method
    • PCR
    • General molecular biological techniques including cloning of DNA fragments.
    • Expression of foreign genes in E. coli.

    Protein work
    • Purification of protein by chromatography
    • SDS-PAGE
    • Western blot analysis.
    • Purification by Gel filtration.
    • Thermodynamic characterization of enzymes with respect to pH, temperature and time.
    • Study of enzyme kinetics(Km Vmax, Ea, Ed, half life etc)
    • CD analysis of protein structure and further analysis of protein structure by DICHROWEB software.

    Immunology
    • Immunoelectrophoresis.
    • Agglutination.
    • ELISA.

    Microbiology

    • Isolation of pure culture
    • Microbiological examination of water
    • Bacterial growth kinetics and effect of pH & temp
    • Maintenance of xenic and axenic strains of Entamoeba histolytica (HM1-IMSS, NIH), Entamoeba dispar, Giardia lamblia, (PD1 and PD2), Leishmania donovani, and Crithridia spp.

    Bio-informatics
    • local and global alignments
    • perl programming.

    2009-2014: Isolation,purification,characterization and applications of industrially important enzymes obtained from fungal and bacterial species and their interaction with nanoparticles (Dissertation for Ph.D)

    My research work during PhD study dealt with isolation, purification, characterization and applications of industrially important enzymes obtained from fungal and bacterial species and their interaction with nanoparticles.
    Purified pectate lyase from an attenuated (APL) and a wild type (WT) strain of Macrophomina phaseolina were supplemented with hydroxyapatite nanoparticles and the changes in their biophysical parameters were monitored. The purified enzyme from the attenuated strain of

    Macrophomina phaseolina showed feeble activity at 50 ºC and pH 5.6. However, on addition of 10.5 µg/ml of hydroxyapatite nanoparticles (NP), APL activity increased 27.7 fold with a 51 fold increase in half-life at a temperature of 90 ºC as compared to untreated APL. The chaperon like activity of NP was evident from entropy–enthalpy compensation profile of APL. The upper critical temperature for such compensation was elevated from 50 ºC to 90 ºC in presence of NP. This dual role of NP in enhancing activity and conferring thermostability to a functionally impaired enzyme is being reported for the first time.

    Purified bacterial cellulase and xylanase were activated in the presence of calcium hydroxyapatite nanoparticles (NP) with concomitant thermostability. The activity and half-life of NP-cellulase was enhanced by 2.5 and 36 folds respectively at a temperature of (80°C).Similarly, The activity and half-life of NP-xylanase was enhanced by 2.1 and 38 folds respectively at a temperature of (80°C). NP-xyl retained 99 % of its activity at the end of two hours (incubation at 80 °C) the corresponding values for this system at the end of 4 hours and 6 hours of incubation were 93 % and 84 % respectively. For the NP-cel system 100 % retention of activity was observed at end of 2 hours of incubation at 80 °C with 95 % retention in activity at the end of 4 hours and 89 % activity retention after 6 hours of incubation. Thus a loss of only 11 % activity was seen at the end of 6 hours. On the other hand for the xylanase (-NP) and cellulase (-NP) there was a drop in activity of 45 % and 30 % respectively under the conditions as above .About 35 % increment in the production of D-xylose and reducing sugars from RH and RS was obtained at 80°C by the sequential treatment of xylanase and cellulase enzymes in presence of NP compared to the untreated enzyme sets. Our findings suggested that,if the Rice Husk and the Rice Straw samples were pre-treated with xylanase prior to treatment with cellulase, the percentage increase of reducing sugar per 100 gms of substrate ( starting material) was enhanced by about 29 % and 41% respectively. These findings can be utilized for the extraction of
    reducing sugars from cellulose and xylan containing waste material. The purely enzymatic

    extraction procedure can be substituted for the harsh and bio-adverse chemical methods.

    RESEARCH EXPERIENCE(During MSc)

    • Research Assistant at the ‘National Institute Of Cholera And Enteric Diseases ’, Under (Indian Council Of Medical Research) Kolkata, India as a part of my summer project (3 rd June 2007 – 3 rd August 2007).
    PROJECT TITLE –“Studies on excretory secretory proteins of entamoeba histolytica”.
    PROJECT SUPERVISOR- Dr. Sandipan Ganguly, Scientist, Molecular parasitology Division
    Work involved laboratory based experimentation and theoretical analysis. It dealt with primarily,
    1) Maintenance of axenic cultures of Entamoeba histolytica and detection of most virulent strain (by different biochemical and biological parameters) for future studies.
    2) To select a medium in which the trophozoites could survive for a longer period of time without further growth.
    3) Preparation of excretory-secretory antigen and kinetics of release of excretory-secretory antigen: To see how early and upto how long the antigen could be released into the medium without cell mortality.
    4) Biochemical characterization of the excretory-secretory antigen: Protein and carbohydrate contents, physico-chemical nature, SDS PAGE protein profile etc.
    5) Role of excretory-secretory antigens in pathogenesis: Proteolytic activity and class of protease, identification of proteolytic polypeptides by zymography, effect of ESP on different substrates important for human amoebiasis and cytotoxic effects on tissue culture and study the signals elicited in target cell death

    • (SHORT TERM PROJECT OF 10 WEEKS DURATION AND SUBMISSION OF PROJECT REPORT DURING THE 4TH SEMESTER)

    PROJECT TITLE – “Synthesis and Characterization of stable gold nanoconjugates with biologically compatible polymers – An approach to nanocarriers”
    PROJECT SUPERVISOR : Prof. Anjan Dasgupta, Head of the Department, Department Of Biochemistry, University of Calcutta, Kolkata, India
    ABSTRACT-

    Drug low solubility and stability in physiological environment constitutes a main hurdle in attaining the appropriate bioavailability. Several polymer-based nanotechnologies are being intended in order to optimize the technological (e.g., solubility, stability, bioavailability, etc.) aspects of drugs. Among them, polymeric nanoparticles appear as one of the most attractive and promising. Gold nanoparticles find application in drug and gene delivery. Formation of biocompatible, stable gold nanoconjugates with biologically compatible polymers has been reported. Modified polyethyleneglycol (PEG) is one such polymer that has been extensively used for this purpose. In this study we have synthesized gold nanoparticles conjugated with a PEG and polyvinylpyrrolidone (PVP) in aqueous solution both by coating and seeding of gold nanoparticles with these polymers to elucidate the properties of the PVP- gold nanoconjugates and compare them with those of more popular PEG- gold nanoconjugates. Characterization of these nanoconjugates have involved the measuring of particle size by dynamic light scattering, measurement of zeta potential of the solutions containing polymer conjugated GNPs and mobility of particles by electrophoretic light scattering and agarose gel electrophoresis. The effect of variation in ionic strength of the medium on the stability of polymer seeded GNPs has also been analyzed. We have found that the stability of polymer seeded GNPs in suspension are greater than their coated counterparts. Variation in absorption spectra, and much largely size and zeta potential of the synthesized GNPs are dependent on the nature and concentrations of the polymer used for coating or seeding. PVP as a whole shows better potential for forming both stable coated and seeded GNPs compared to PEG. However the biocompatibility of PVP conjugated GNPs and its efficacy to serve as a better nanocarrier is a question to be answered only through further investigations. The present study has therefore provided the preliminary platform for further exploration of the potential of PVP conjugated GNPs to emerge as efficient nanocarriers in near future.

    • Most acceptable biocompatible nanoconjugate: ( Surface functionalization of gold nanoparticles)
    PROJECT SUPERVISOR :Prof. Anjan Dasgupta, Head of the Department,Department Of Biochemistry, University of Calcutta, Kolkata, India

    ABSTRACT:
    Colloidal functionalised nanoparticles have attracted tremendous attention over the last decade, owing to its distinguished roles in fundamental and technical applications.
    Here we hypothesized to coat gold nanoparticles with different biomolecules to recognize the binding pattern of those molecules, to gold nanoparticles and thus paving the way to coat nanoparticles with more complex polymer in future.
    We used amino acids, namely lysine, cysteine, arginine etc to bind the gold nanoparticles and alter the surface chemistry. The stability of nanoparticles, when coated with these biomolecules were analysed via various means and the properties of nanoparticles after coating were observed using several biochemical tests.
    It was found that in most of the cases, the nanoparticles were successfully coated, and they remained stable in that condition. The stability was analysed by calculating zeta potentials and the size was measured by Dynamic Light Scattering (DLS). Spectrophotometric data also strengthened the point and finally electrophoresis of those coated particles showed that the nanoparticles when covered with amino acids separated in the gel according to the respective amino acids.

    References:

    1. Prof. Krishanu Chakraborty (Principal Investigator)
    Department of Biochemistry,
    University of Calcutta
    35, Ballygunge Circular Road,
    Kolkata-700019, West Bengal, India.
    Cell: +91 9831535059
    e-mail: krishchak@hotmail.com
    http://biochem.caluniv.in/index.htm

    2. Prof. Anjan Kr. Dasgupta
    Department of Biochemistry,
    University of Calcutta
    35, Ballygunge Circular Road,
    Kolkata-700019, West Bengal, India.
    Fax: +91 33 2461 4849
    Cell: +91 9748758663
    e-mail: adgcal@gmail.com
    http://biochem.caluniv.in/index.htm

    3. Dr. Debasish Bhattacharyya
    Senior Principal Scientist
    Structural Biology & Bio-Informatics Division
    CSIR-IICB
    4, Raja S.C. Mullick Road,
    Kolkata-700 032, West Bengal, India
    debasish@iicb.res.in

    4. Prof. D. J. Chattopadhyay
    Pro-Vice Chancellor (Academic)
    Department of Biochemistry,
    University of Calcutta
    35, Ballygunge Circular Road,
    Kolkata-700019, West Bengal, India.
    Fax: +91 33 2461 4849
    Cell: +91 9831083791
    e-mail: dhrubajyotic@gmail.com
    http://biochem.caluniv.in/index.htm

    “I hereby declare that the information furnished above is true to the best of my knowledge and belief”.

  • CURRICULUM VITAE

    Praveen Kumar Mehta

    Corresponding address
    C/O Sh. Inder Singh Mehta
    District and Session Judge
    D-2/1 Court Lane,
    Civil lines Delhi-110054
    Email-mehtapkbiotech@gmail.com
    Mobile- +91 8130930722

    Personal Details
    Date of Birth 15 March, 1982
    Marital status Married
    Nationality Indian
    Languages Known English, Hindi
    Hobbies Painting, Photography, Rock Climbing

    OBJECTIVE
    A full-time position (such as Postdoctoral, or Research Scientist) in Biochemical engineering, Biological chemistry, Medicinal or Enzyme Biotechnology or Biocatalysis

    Educational qualifications

    • Ph.D. (Biotechnology), Himachal Pradesh University (HPU), Shimla, India (2013).
    • M.Sc. (Biotechnology), Himachal Pradesh University, Shimla, India (2004-2006).
    • B.Sc. (G) Deen Dyal Upadhyaya college, Delhi University (DU), India (2000-2003)

    Professional Training & Research Experience:
    • Ph.D. from Department of Biotechnology, Himachal Pradesh University, Shimla, India under the guidance of Prof. Tek Chand Bhalla entitled “Isolation, characterization and application of thermostable amidase of Geobacillus sp. RL-2a” (2013).
    • Hands on training in the UGC-NRCBS summer school training programme on “PCR applications” organized by NRCBS centre of Madurai Kamraj University during 17th March-31st March 2010.
    • I underwent two month external internship on project entitled “Cloning and expression of GS from Camellia sinensis” under the direct supervision of Dr. Sudesh kumar, scientist (Biotechnology) at Institute of Himalayan Bioresource Technology, Palampur (H.P).
    • I did M.Sc. Project entitled “Isolation of xylanase producing organism and optimization of reaction conditions” under the direct supervision of Dr. Duni Chand, Dept of Biotechnology, HPU, Shimla
    • Participated in the DBT, Govt. of India sponsored Workshop-cum Training Programme on “Tools and Techniques for Nucleic Acid and Protein Sequence Analysis” organized by Bioinformatics centre, Himachal Pradesh University, Summer hill, Shimla.
    Awards and achievements
    • Qualified for National Overseas scholarship (NOS), Govt. of India (2012-2013) for Post Doctoral research in biotechnology.
    • Qualified UGC (NET) – June 2007 All India National Level Test examination essential for Junior Research Fellowship and Lecturership in Indian Universities
    • Qualified DBT-JRF – June 2007 All India National Level Test examination essential for Junior Research Fellowship (DBT)
    • Qualified UGC (NET) – June 2006 All India National Level Test examination essential for Lectureship-category in Indian Universities
    • Awarded Scholarships from Department of Biotechnology (DBT), Govt. of India (July 2004-June 2006) for M. Sc. Biotechnology programme.

    Publications:
    Mehta P.K, Bhatia S.K, Bhatia R.K and Bhalla T.C. (2014) Bench scale production of nicotinic acid using a versatile amide-hydrolysing Geobacillus subterraneus RL-2a isolated from thermal spring of Manikaran, Journal of Molecular Catalysis B: Enzymatic. 10: 58-65.
    Bhatia S.K, Mehta P.K, Bhatia R.K and Bhalla T.C. (2014) Optimization of arylacetonitrilase production from Alcaligenes sp. MTCC 10675 and its application in mandelic acid synthesis. Applied Microbiology and Biotechnology. 98: 83-94.
    Mehta P.K, Bhatia S.K, Bhatia R.K and Bhalla T.C. (2013) Purification and characterization of a novel thermo-active amidase from Geobacillus subterraneus RL-2a. Extremophile. 17: 637-48.
    Bhatia S.K, Mehta P.K, Bhatia R.K and Bhalla T.C. (2013) Purification and characterization of arylacetonitrile specific nitrilase of Alcaligenes sp. MTCC 10675. Biotechnology and Applied Biochemistry. DOI:10.1002/bab.1192
    Bhatia R.K, Bhatia S.K, Mehta P.K, Bhalla TC. (2013) Production and characterization of acyl transfer activity of amidase from Alcaligenes sp. MTCC 10674 for Synthesis of Hydroxamic Acids. Journal of Microbial and Biochemical Technology. 5:1
    Bhatia S.K, Mehta P.K, Bhatia R.K and Bhalla T.C. (2013) Purification of bienzymatic system: nitrile hydratase and amidase of Alcaligenes sp. MTCC 10674. 3 Biotech DOI 10.1007/s13205-013-0163
    Bhatia S.K, Mehta P.K, Bhatia R.K and Bhalla T.C (2012). An isobutyronitrile induced bienzymatic system of Alcaligenes sp. MTCC 10674 and its application for the synthesis of α-hydroxyisobutyric acid. Bioprocesss and Biosystem Engineering. 36: 613-25
    Bhatia R.K, Bhatia S.K, Mehta P.K and Bhalla TC (2012) Bench scale production of benzohydroxlic acid using acyl transferase activity from Alcaligenes sp. MTCC 10674. Journal of Industrial Microbiology and Biotechnology. 40: 21-7.

    Book Chapter
    Bhalla TC, Mehta P K, Bhatia SK, Thakur N and Pratush A (2008) Microorganisms for Food and Feed. In: Fundamentals of Food Biotechnology, Anne Publisher, New Delhi.
    Techniques learned/Equipments handled:
    Isolation, characterization and maintenance of bacterial cultures, identification of bacteria, screening of microbes for industrial enzymes, strain preservation (lyophilization), Protein purification and characterization, Gel permeation, ion exchange & affinity chromatography, SDS & native PAGE, Microbiological techniques, Technological combination, Various Chromatographic techniques like HPLC, GC, Lyophilizer, Bead beater, Sonicator, Microscopy, Fermenter, Transformation of E. coli, genomic DNA isolation from bacteria, plant & blood, plasmid isolation, restriction digestion & ligation of DNA, DNA amplification by PCR, Assay of various enzyme, immobilization of enzymes, Computer skill: Internet, Microsoft Word, Excel, Power Point, Publisher, Adobe Photoshop, SigmaPlot, ChemSketch, BLAST, ClustalW, ORF searchingTravelling
    Teaching Experience
    Practical demonstration of Enzyme technology, Recombinant DNA Technology experiments to M. Sc. Biotechnology students.

    Poster presentation
    Bhalla TC, Mehta PK, Sharma NN and Bhatia SK (2009) Production of isonicotinic acid using agar entrapped whole cells of Nocardia globerula NHB-2. In: XVII International Conference on Bioencapsulation, 24-26 Sept. Groningen, Netherlands
    Mehta PK, Bhatia SK, Bhatia RK, Kumar V and Bhalla T C (2011) Thermostable amidase from Geobacillus sp. RL-2A: isolation and screening. In: 52nd annual conference of Association of Microbiologist of India on internal conference on microbial biotechnology for sustainable development. 3-6 Nov. Chandigarh, India.
    Mehta PK, Bhatia SK, Bhatia RK, Kumar V and Bhalla TC. Optimization of production conditions for biosynthesis of nicotinic acid from nicotinamide using resting cells of thermostable Geobacillus sp. RL-2a. Poster presented in International conference on Advances in Biological Sciences (ICABS-2012) March 15-17, 2012. Department of Biotechnology and Microbiology, Kannur, Kerala, India.

    Referees
    • Prof. Tek Chand Bhalla, Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171005, email: bhallatc@rediffmail.com
    Telephone: +91-177-2831948, Telefax: +91-177-2832154
    • Prof. Duni Chand, Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171005, email: dunichand_2000@yahoo.com
    Tel./fax: +91 177 2831948
    • Dr. Arvind Kumar Bhatt, Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171005, email: bhattak08@gmail.com
    Phone/Fax: 91-177-2831948/2832153

    Declaration
    I hereby declare that all the information given above is true to the best of my knowledge.

    Delhi, India
    May 15, 2014 (Praveen Kumar Mehta)

  • Postdoctoral positions in Peptide/Protein Chemistry, Enzymology,Molecular Biology, and Glycobiology

    Postdoctoral positions are available to work on an interdisciplinary research program in bioorganic chemistry and glycobiology at University of Maryland School of Medicine. The candidates will participate in one or more of the following research projects: 1) development of chemoenzymatic methods for glycoprotein synthesis; 2) glycosylation engineering of monoclonal antibodies and other therapeutic proteins; 3) HIV glycobiology and vaccine design. We are particularly interested in candidates with strong background in peptide/protein chemistry, enzymology, oligosaccharide chemistry, molecular biology, oligosaccharide chemistry, and/or glycobiology. Knowledge in vaccine design and immunization is a plus.

    For considerations, please email your CV, list of publications, and names of three references to Dr. Lai-Xi Wang, Institute of Human Virology, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201.
    Email: LWang@som.umaryland.edu
    Website: http://www.ihv.org/research/carbohydrate.html

  • Seneviratne Samaratunga
    Department of Chemistry, University of Maine,
    Orono, Maine 04469
    Phone: (207) 581-1194 office, (573) 639-1666 cell
    Email: seneviratne_samaratunga@umit.maine.edu
    CITIZENSHIP: United States of America

    OBJECTIVE
    A full-time position (such as Postdoctoral, Assistant Professor, or Research Scientist) in Biophysical, Biological, Medicinal or Analytical Chemistry, or in Nanoscience.

    EDUCATION
    Ph.D., Biophysical Chemistry 6/2008 – 5/2013 (expected)
    Department of Chemistry, University of Maine, Orono , USA
    GPA: 3.9/4.0

    B.S. (Honors) in Chemistry (US equivalent) , University of Peradeniya, Sri Lanka (1/1989-1/1993) GPA: 3.3/4.0

    Grad Chem , Institute of Chemistry, Sri Lanka (5/1996-6/1997)
    GPA: 3.6/4.0

    Related Courses
    Quantum Chemistry, Statistical Thermodynamics, Structure and Mechanism in Biological Chemistry, Inorganic Chemistry Kinetics and Mechanism, Topics in Advanced Inorganic Chemistry, Topics in Chemistry (Spectroscopy), Mathematical Methods in Physics , Chemistry Instructional Laboratory Leadership, Computer Simulation Methods, and Introduction to Computing Using FORTRAN.

    TEACHING EXPERIENCE
    Graduate Teaching Assistant, Department of Chemistry, University of Maine – Orono
    (6/2008-present), Lab Instructor for General Chemistry.

    Tutor (Chemistry and Physics), University of Missouri – Columbia (8/2005-5/2008)
    Conducted tutorial classes and help undergraduates to prepare their laboratory classes (Chemistry 1100/1310/1320/1330, Organic Chemistry 2100/2010/2050, Radio Chemistry, Quantitative Analytical Methods, University Physics I and II).

    Assistant Lecturer , University of Peradeniya, Sri Lanka (1/1993-12/1993) Duties included conducting lectures and laboratory classes as well as grading for chemistry undergraduates.

    High School Teacher, Kandy International School, Sri Lanka
    (1/1995 – 12/1995) Taught Chemistry and Physics.

    Seneviratne Samaratunga seneviratne_samaratunga@umit.maine.edu

    INDUSTRIAL EXPERIENCE
    Deputy Manager Technical Services/Senior Chemist, Main Laboratory, Ceylon Petroleum Corporation, Sri Lanka (3/1996-12/2002)
    Supervised, planned, and managed technical and administrative functions for the laboratory.

    Technical Lab Specialist, Applied Technology Group, Columbia, Missouri, USA (6/2001-1/2002 while on leave from Ceylon Petroleum Corporation ). Duties included technical formulation studies, and volumetric measuring of complex materials and resin coatings. Much of this work involved precise use of volumetric and digital scale equipment, and good judgment in process work on formula calculation.

    Chemist , Hayleys Group, Sri Lanka (1/1994-5/1995)
    Conducted product development for food exporter, including developing formulas for new products, improving palatability of existing products and establishing specifications for finished products and raw ingredients. Coordinated and led microbiology, nutrition, analytical chemistry and pilot plant teams. Provided technical leadership for supply chain process improvements, cost reductions and quality improvements.

    Phlebotomist, Boone Hospital Center, Columbia, Missouri, USA (2007-2008)
    Primarily performed phlebotomy (drawing blood) for Boone Hospital client inpatients and outpatients. Responsible for all aspects of processing laboratory specimens, including distribution within the Boone Hospital Laboratory and testing sent to outside laboratories.

    RESEARCH EXPERENCE
    Hydration and confinement effects on helix formation in a 23- residue polypeptide inside carbon nanotubes to mimic the effect of ribosome tunnel.
    Department of Chemistry, University of Maine, Orono (6/2008-present)

    Enzyme separation using bio-electroanalytical methods.
    University of Peradeniya, Sri Lanka (1/1992-1/1993)

    Research Assistant, developing a wastewater treatment method using catalysts.
    Tokyo Institute of Technology, Japan (1995-1996)

    Professional Affiliations
    Member of the American Chemical Society (5/2010-present)

    Lifetime member of the Institute of Chemistry, Sri Lanka (M.I. Chem, Chartered Chemist)

    Lifetime member of the Sri Lanka Association for the Advancement of Science (SLAAS)

    Lifetime member of the Sri Lanka Professional Organization

    Seneviratne Samaratunga seneviratne_samaratunga@umit.maine.edu

    PRESENTATIONS
    • “Solvents in green chemistry.” Presented 1/24/2012, Department of Chemistry,
    University Of Maine, Orono
    • “New membranes and catalysts for fuel cells.” Presented 2/15/2011, Department of Chemistry, University Of Maine, Orono
    • Poster presentation, “Helix Formation in Polypeptides Confined to Carbon Nanotubes.” Presented 8/24/2010 at ACS Meeting, Boston Convention Center
    • “Helix Formation in Polypeptides Confined to Carbon Nanotubes.” Presented 3/16/2010, Department of Chemistry, University Of Maine, Orono
    • Presentation on International Standards (Petroleum Industry) to the Sri Lanka Air Force.
    Presented 1/24/2001 at Ceylon Petroleum Corporation
    WORKSHOPS ATTENDED
    • Using Free-Response Questions to Probe Student Thinking – workshop for STEM graduate teaching assistants (3/14/2012)
    • Inquiry-Based Labs – workshop for STEM graduate teaching assistants (2/2/2012)
    • Simulating Membrane Channels (Potassium Channels) – online workshop conducted by University of Illinois (8/1/ 2011 to 8/4/2011). Limited to 10 participants.
    The workshop broadened my understanding of concepts and principles in the field of Computational and Theoretical Biophysics to simulate potassium channels.

    RESEARCH INTERESTS
    • Hydration and confinement effects on helix formation in polypeptide with 23 or more residues inside carbon nanotubes to mimic the effect of ribosome tunnel, and study of related diseases.
    • Experimental, theoretical and computational techniques for biophysical problems in drug design and delivery using carbon nanotubes. Special interest in the membrane proteins known as ion channels or potassium channels, essential for electrical and chemical activity in all organisms and responsible for many neurological and cardio-vascular disorders. Microscopic driving forces of protein function in cell membranes.
    • Structure characterization of biological systems and their dynamics, protein-membrane interactions, structure and stability of neat and heterogeneous clusters, solvation dynamics of ions in water clusters, solvent models, relaxation dynamics of solvents.

    COMPUTER SKILLS:
    Operating systems: UNIX, Linux, Windows
    Computer language: FORTRAN
    Computing software: Amber, Gaussian, NAMD
    Graphic software: Gnuplot, Origin, VMD
    Scientific applications: MATLAB
    Other software: EndNote, MathType

    Seneviratne Samaratunga seneviratne_samaratunga@umit.maine.edu

    PUBLICATIONS
    1) S. Samaratunga, D. Suvlu, D. Thirumalai and J.C. Rasaiah “Hydration and confinement effects on helix formation in a 23- residue polypeptide” Biophysical Journal. Manuscript in preparation.

    2) S. Samaratunga, D. Thirumalai and J.C. Rasaiah “Polypeptides in chiral carbon nanotube” Journal of Chemical Physics. Manuscript in preparation.

    REFERENCES
    1. JAYENDRAN C. RASAIAH
    Professor of Chemistry
    Work Address: University of Maine
    Voice: (207) 581-1179
    E-mail: rasaiah@maine.edu

    2. HOWARD H. PATTERSON
    Professor of Chemistry
    Work Address: University of Maine
    Voice: (207) 581-1178
    E-mail: howardp@maine.edu

    3. SCOTT COLLINS
    Professor; Cooperating Professor of Mechanical Engineering, Cooperating Professor of
    Biochemistry, Microbiology, and Molecular Biology
    Work Address: University of Maine
    Voice: (207)-581-2269
    E-mail: scott.collins@maine.edu

  • RESEARCH ASSOCIATE POSITION
    Miziorko Laboratory, University of Missouri-Kansas City

    A Ph.D.scientist position is available (biochemistry, chemistry, structural biology, or medicinal chemistry background) to investigate enzymes of mevalonate metabolism/isoprenoid biosynthesis. Recombinant forms of these enzymes from animals and human pathogens (protozoa; bacteria) are available. Additionally, tight binding inhibitors (metabolite analogs) are available. Drug-like compounds will also be evaluated to determine specificity and potency. Appropriate compounds will support collaborative structural work on enzyme-inhibitor complexes, leading to expanded structure/function studies. A preferred candidate will have experience with chemical synthesis of enzyme substrates/inhibitors, enzyme assays, structural biology, or computational approaches to study enzyme-ligand complexes. Salary is commensurate with experience ($40,000-47,000 per year). Candidates should forward an application letter, CV and contact information for professional references to:

    Dr. Henry Miziorko
    Marion-Merrell-Dow Professor
    Division Head, Molecular Biology & Biochemistry
    University of Missouri-Kansas City
    5007 Rockhill Road
    Kansas City, MO 64110

    miziorkoh@umkc.edu

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