Acetylation of Aurora B by TIP60 ensures accurate chromosomal segregation

February 1st, 2016 by Fei Mo

Nature Chemical Biology 12, 226 (2016). doi:10.1038/nchembio.2017

Authors: Fei Mo, Xiaoxuan Zhuang, Xing Liu, Phil Y Yao, Bo Qin, Zeqi Su, Jianye Zang, Zhiyong Wang, Jiancun Zhang, Zhen Dou, Changlin Tian, Maikun Teng, Liwen Niu, Donald L Hill, Guowei Fang, Xia Ding, Chuanhai Fu & Xuebiao Yao

  • Posted in Nat Chem Biol, Publications
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Discovery of tumor-specific irreversible inhibitors of stearoyl CoA desaturase

February 1st, 2016 by Panayotis C Theodoropoulos

Nature Chemical Biology 12, 218 (2016). doi:10.1038/nchembio.2016

Authors: Panayotis C Theodoropoulos, Stephen S Gonzales, Sarah E Winterton, Carlos Rodriguez-Navas, John S McKnight, Lorraine K Morlock, Jordan M Hanson, Bethany Cross, Amy E Owen, Yingli Duan, Jose R Moreno, Andrew Lemoff, Hamid Mirzaei, Bruce A Posner, Noelle S Williams, Joseph M Ready & Deepak Nijhawan

  • Posted in Nat Chem Biol, Publications
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Assembly and clustering of natural antibiotics guides target identification

February 1st, 2016 by Chad W Johnston

Nature Chemical Biology 12, 233 (2016). doi:10.1038/nchembio.2018

Authors: Chad W Johnston, Michael A Skinnider, Chris A Dejong, Philip N Rees, Gregory M Chen, Chelsea G Walker, Shawn French, Eric D Brown, János Bérdy, Dennis Y Liu & Nathan A Magarvey

  • Posted in Nat Chem Biol, Publications
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A pyridoxal phosphate–dependent enzyme that oxidizes an unactivated carbon-carbon bond

January 25th, 2016 by Yi-Ling Du

Nature Chemical Biology 12, 194 (2016). doi:10.1038/nchembio.2009

Authors: Yi-Ling Du, Rahul Singh, Lona M Alkhalaf, Eugene Kuatsjah, Hai-Yan He, Lindsay D Eltis & Katherine S Ryan

  • Posted in Nat Chem Biol, Publications
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A cellular chemical probe targeting the chromodomains of Polycomb repressive complex 1

January 25th, 2016 by Jacob I Stuckey

Nature Chemical Biology 12, 180 (2016). doi:10.1038/nchembio.2007

Authors: Jacob I Stuckey, Bradley M Dickson, Nancy Cheng, Yanli Liu, Jacqueline L Norris, Stephanie H Cholensky, Wolfram Tempel, Su Qin, Katherine G Huber, Cari Sagum, Karynne Black, Fengling Li, Xi-Ping Huang, Bryan L Roth, Brandi M Baughman, Guillermo Senisterra, Samantha G Pattenden, Masoud Vedadi, Peter J Brown, Mark T Bedford, Jinrong Min, Cheryl H Arrowsmith, Lindsey I James & Stephen V Frye

  • Posted in Nat Chem Biol, Publications
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A two-state activation mechanism controls the histone methyltransferase Suv39h1

January 25th, 2016 by Manuel M Müller

Nature Chemical Biology 12, 188 (2016). doi:10.1038/nchembio.2008

Authors: Manuel M Müller, Beat Fierz, Lenka Bittova, Glen Liszczak & Tom W Muir

  • Posted in Nat Chem Biol, Publications
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Nutrient-Regulated Phosphorylation of ATG13 Inhibits Starvation-Induced Autophagy [Membrane Biology]

January 22nd, 2016 by Puente, C., Hendrickson, R. C., Jiang, X.

Autophagy is a conserved catabolic process that utilizes a defined series of membrane trafficking events to generate a de novo double-membrane vesicle termed the autophagosome, which matures by fusing to the lysosome. Subsequently, the lysosome facilitates the degradation and recycling of the cytoplasmic cargo. In yeast, the upstream signals that regulate the induction of starvation-induced autophagy are clearly defined. The nutrient-sensing kinase Tor inhibits the activation of autophagy by regulating the formation of the Atg1-Atg13-Atg17 complex, through hyperphosphorylation of Atg13. However, in mammals, the ortholog complex ULK1-ATG13-FIP200 is constitutively formed. As such, the molecular mechanism by which mTOR regulates mammalian autophagy is unknown. Here we report the identification and characterization of novel nutrient-regulated phosphorylation sites on ATG13: Ser224 and Ser258. mTOR directly phosphorylates ATG13 on Ser258 while Ser224 is a putative AMPK phosphorylation site. In ATG13 knockout cells reconstituted with an unphosphorylatable mutant of ATG13, ULK1 kinase activity is more potent, and amino acid starvation induced more rapid ULK1 translocation and autophagy. Therefore, ATG13 phosphorylation plays a crucial role in autophagy regulation.

Real-Time Observation of Backtracking by Bacterial RNA Polymerase

January 21st, 2016 by Agnieszka Lass-Napiorkowska and Tomasz Heyduk

TOC Graphic

Biochemistry
DOI: 10.1021/acs.biochem.5b01184

Neurobiology: Tracking the scent

January 19th, 2016 by Catherine Goodman

Nature Chemical Biology 12, 53 (2016). doi:10.1038/nchembio.2011

Author: Catherine Goodman

Metalloenzymes: Getting the gold

January 19th, 2016 by Catherine Goodman

Nature Chemical Biology 12, 53 (2016). doi:10.1038/nchembio.2014

Author: Catherine Goodman