Elucidation of gibberellin biosynthesis in bacteria reveals convergent evolution

November 14th, 2016 by Ryan S Nett

Nature Chemical Biology 13, 69 (2017). doi:10.1038/nchembio.2232

Authors: Ryan S Nett, Mariana Montanares, Ariana Marcassa, Xuan Lu, Raimund Nagel, Trevor C Charles, Peter Hedden, Maria Cecilia Rojas & Reuben J Peters

  • Posted in Nat Chem Biol, Publications
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Ferroptosis: Oxidized PEs trigger death

November 14th, 2016 by Katharina D'Herde

Nature Chemical Biology 13, 4 (2017). doi:10.1038/nchembio.2261

Authors: Katharina D'Herde & Dmitri V Krysko

Ferroptosis is characterized by accumulation of lipid peroxidation products and lethal ROS, but the source and identity of lipid death signals that cause toxicity are poorly defined. New studies reveal that ACSL4 controls sensitivity to ferroptosis and that oxidized phosphatidylethanolamines are critical for ferroptosis execution.

ACSL4 dictates ferroptosis sensitivity by shaping cellular lipid composition

November 14th, 2016 by Sebastian Doll

Nature Chemical Biology 13, 91 (2017). doi:10.1038/nchembio.2239

Authors: Sebastian Doll, Bettina Proneth, Yulia Y Tyurina, Elena Panzilius, Sho Kobayashi, Irina Ingold, Martin Irmler, Johannes Beckers, Michaela Aichler, Axel Walch, Holger Prokisch, Dietrich Trümbach, Gaowei Mao, Feng Qu, Hulya Bayir, Joachim Füllekrug, Christina H Scheel, Wolfgang Wurst, Joel A Schick, Valerian E Kagan, José Pedro Friedmann Angeli & Marcus Conrad

  • Posted in Nat Chem Biol, Publications
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Tunable thermal bioswitches for in vivo control of microbial therapeutics

November 14th, 2016 by Dan I Piraner

Nature Chemical Biology 13, 75 (2017). doi:10.1038/nchembio.2233

Authors: Dan I Piraner, Mohamad H Abedi, Brittany A Moser, Audrey Lee-Gosselin & Mikhail G Shapiro

  • Posted in Nat Chem Biol, Publications
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A Conserved Tripeptide Sequence at the C-terminus of the Poxvirus DNA Processivity Factor D4 is Essential for Protein Integrity and Function [Microbiology]

November 11th, 2016 by Nuth, M., Guan, H., Ricciardi, R. P.

Vaccinia virus (VACV) is a poxvirus member, and the VACV D4 protein serves both as a uracil-DNA glycosylase (UDG) and as an essential component required for processive DNA synthesis. The VACV A20 protein has no known catalytic function itself, but associates with D4 to form the D4-A20 heterodimer that functions as the poxvirus DNA processivity factor. The heterodimer enables the DNA polymerase to efficiently synthesize extended strands of DNA. Upon characterizing the interaction between D4 and A20, we observed that the C-terminus of D4 is susceptible to perturbation. Further analysis demonstrated that a conserved hexapeptide stretch at the extreme C-terminus of D4 is essential for maintaining protein integrity, as assessed by its requirement for the production of soluble recombinant protein that is functional in processive DNA synthesis. From the known crystal structures of D4, the C-terminal hexapeptide is shown to make intramolecular contact with residues spanning the protein's inner core. Our mutational analysis revealed that a tripeptide motif (215-GFI-217) within the hexapeptide comprises apparent residues necessary for the contact. Prediction of protein disorder identified the hexapeptide and several regions upstream of Gly-215 that comprise residues of the interface surfaces of the D4-A20 heterodimer. Our study suggests that 215-GFI-217 anchors these potentially dynamic upstream regions of the protein in order to maintain protein integrity. Unlike UDGs from diverse sources, where the C-termini are disordered and do not form comparable intramolecular contacts, this feature may be unique to orthopoxviruses.
  • Posted in Journal of Biological Chemistry, Publications
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Cyanobacterial Surface Display System Mediates Engineered Interspecies and Abiotic Binding

November 9th, 2016 by Derek T. Fedeson and Daniel C. Ducat

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.6b00254

Reproducibility of High-Throughput Plate-Reader Experiments in Synthetic Biology

November 9th, 2016 by Michael Chavez, Jonathan Ho and Cheemeng Tan

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.6b00198

Temporal Analysis of PP2A Phosphatase Activity During Insulin Stimulation Using a Direct Activity Probe

November 7th, 2016 by Jon R. Beck, Tiffany Truong and Cliff I. Stains

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ACS Chemical Biology
DOI: 10.1021/acschembio.6b00697
  • Posted in ACS Chemical Biology, Publications
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Host Cell Interactions Are a Significant Barrier to the Clinical Utility of Peptide Antibiotics

November 7th, 2016 by Charles G. Starr, Jing He and William C. Wimley

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ACS Chemical Biology
DOI: 10.1021/acschembio.6b00843

Inhibition of RAS function through targeting an allosteric regulatory site

November 7th, 2016 by Russell Spencer-Smith

Nature Chemical Biology 13, 62 (2017). doi:10.1038/nchembio.2231

Authors: Russell Spencer-Smith, Akiko Koide, Yong Zhou, Raphael R Eguchi, Fern Sha, Priyanka Gajwani, Dianicha Santana, Ankit Gupta, Miranda Jacobs, Erika Herrero-Garcia, Jacqueline Cobbert, Hugo Lavoie, Matthew Smith, Thanashan Rajakulendran, Evan Dowdell, Mustafa Nazir Okur, Irina Dementieva, Frank Sicheri, Marc Therrien, John F Hancock, Mitsuhiko Ikura, Shohei Koide & John P O'Bryan

  • Posted in Nat Chem Biol, Publications
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