A fluorescent probe for cysteine depalmitoylation reveals dynamic APT signaling

December 19th, 2016 by Rahul S Kathayat

Nature Chemical Biology 13, 150 (2017). doi:10.1038/nchembio.2262

Authors: Rahul S Kathayat, Pablo D Elvira & Bryan C Dickinson

Hundreds of human proteins are modified by reversible palmitoylation of cysteine residues (S-palmitoylation), but the regulation of depalmitoylation is poorly understood. Here, we develop 'depalmitoylation probes' (DPPs), small-molecule fluorophores, to monitor the endogenous activity levels of 'erasers' of S-palmitoylation, acylprotein thioesterases (APTs). Live-cell analysis with DPPs reveals rapid growth-factor-mediated inhibition of the depalmitoylation activity of APTs, exposing a novel regulatory mechanism of dynamic lipid signaling.

  • Posted in Nat Chem Biol, Publications
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Inhibitors of Mycobacterium tuberculosis DosRST signaling and persistence

December 19th, 2016 by Huiqing Zheng

Nature Chemical Biology 13, 218 (2017). doi:10.1038/nchembio.2259

Authors: Huiqing Zheng, Christopher J Colvin, Benjamin K Johnson, Paul D Kirchhoff, Michael Wilson, Katriana Jorgensen-Muga, Scott D Larsen & Robert B Abramovitch

  • Posted in Nat Chem Biol, Publications
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Orphan receptor ligand discovery by pickpocketing pharmacological neighbors

December 19th, 2016 by Tony Ngo

Nature Chemical Biology 13, 235 (2017). doi:10.1038/nchembio.2266

Authors: Tony Ngo, Andrey V Ilatovskiy, Alastair G Stewart, James L J Coleman, Fiona M McRobb, R Peter Riek, Robert M Graham, Ruben Abagyan, Irina Kufareva & Nicola J Smith

  • Posted in Nat Chem Biol, Publications
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Molecular insights into the enzyme promiscuity of an aromatic prenyltransferase

December 19th, 2016 by Ridao Chen

Nature Chemical Biology 13, 226 (2017). doi:10.1038/nchembio.2263

Authors: Ridao Chen, Bingquan Gao, Xiao Liu, Feiying Ruan, Yong Zhang, Jizhong Lou, Keping Feng, Carsten Wunsch, Shu-Ming Li, Jungui Dai & Fei Sun

  • Posted in Nat Chem Biol, Publications
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RAS signaling: Divide and conquer

December 16th, 2016 by Matthew Holderfield

Nature Chemical Biology 13, 7 (2017). doi:10.1038/nchembio.2264

Authors: Matthew Holderfield & Deborah K Morrison

The role of RAS dimerization in tumor biology is an emerging area of preclinical cancer research. Characterization of a RAS monobody indicates that dimer formation is required for RAS signaling, revealing the dimer interface as a potential drug target.

Protein folding: Minimizing frustration

December 16th, 2016 by Mirella Bucci

Nature Chemical Biology 13, 1 (2017). doi:10.1038/nchembio.2277

Author: Mirella Bucci

Biocatalysis: Custom carbon cycling

December 16th, 2016 by Caitlin Deane

Nature Chemical Biology 13, 1 (2017). doi:10.1038/nchembio.2275

Author: Caitlin Deane

Neurodegeneration: Untangling tau

December 16th, 2016 by Alison Farrell

Nature Chemical Biology 13, 1 (2017). doi:10.1038/nchembio.2276

Author: Alison Farrell

RNA structure: Widening the probe

December 16th, 2016 by Grant Miura

Nature Chemical Biology 13, 1 (2017). doi:10.1038/nchembio.2274

Author: Grant Miura

Natural products: Mapping an amazing thicket

December 16th, 2016 by Mark E Horsman

Nature Chemical Biology 13, 6 (2017). doi:10.1038/nchembio.2265

Authors: Mark E Horsman & Christopher N Boddy

The Global Natural Products Social Molecular Networking (GNPS) resource and the DEREPLICATOR algorithm provide new tools for analyzing mass spectral data and enabling natural products discovery.