Deep Sequencing Analysis of Aptazyme Variants Based on a Pistol Ribozyme

April 14th, 2017 by Shungo Kobori, Kei Takahashi and Yohei Yokobayashi

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.7b00057

Optogenetics: Lighting up kinases

April 13th, 2017 by Grant Miura

Nature Chemical Biology 13, 451 (2017). doi:10.1038/nchembio.2365

Author: Grant Miura

Bacterial Membranes: Tear down this wall

April 13th, 2017 by Caitlin Deane

Nature Chemical Biology 13, 451 (2017). doi:10.1038/nchembio.2366

Author: Caitlin Deane

Microbiology: A new language for small talk

April 13th, 2017 by Yi-Ming Shi

Nature Chemical Biology 13, 453 (2017). doi:10.1038/nchembio.2362

Authors: Yi-Ming Shi & Helge B Bode

A new signal–receptor pair involved in regulating biofilm formation and virulence was detected in Vibrio cholerae. Both the signal and the transcription factor belong to common classes of natural products and receptor proteins, suggesting widespread importance of related systems in nature.

Intrinsic Disorder: Fuzzy fast feedback

April 13th, 2017 by Mirella Bucci

Nature Chemical Biology 13, 451 (2017). doi:10.1038/nchembio.2367

Author: Mirella Bucci

Targeted protein degradation: You can glue it too!

April 13th, 2017 by Michal J Walczak

Nature Chemical Biology 13, 452 (2017). doi:10.1038/nchembio.2355

Authors: Michal J Walczak, Georg Petzold & Nicolas H Thomä

Proteolysis-targeting chimera (PROTACs) are synthetic molecules that recruit neo-substrate proteins to a ubiquitin ligase for ubiquitination and subsequent degradation. Structural insight into the VHL–MZ1–BRD4 complex reveals how the rationally designed MZ1–PROTAC molecule mediates degradation of an unnatural protein substrate.

Anti-Bacterials: Out-SMARting drug resistance

April 13th, 2017 by Alison Farrell

Nature Chemical Biology 13, 451 (2017). doi:10.1038/nchembio.2368

Author: Alison Farrell

The chemical basis for electrical signaling

April 13th, 2017 by William A Catterall

Nature Chemical Biology 13, 455 (2017). doi:10.1038/nchembio.2353

Authors: William A Catterall, Goragot Wisedchaisri & Ning Zheng

Mechanistic insights into energy conservation by flavin-based electron bifurcation

April 10th, 2017 by Carolyn E Lubner

Nature Chemical Biology 13, 655 (2017). doi:10.1038/nchembio.2348

Authors: Carolyn E Lubner, David P Jennings, David W Mulder, Gerrit J Schut, Oleg A Zadvornyy, John P Hoben, Monika Tokmina-Lukaszewska, Luke Berry, Diep M Nguyen, Gina L Lipscomb, Brian Bothner, Anne K Jones, Anne-Frances Miller, Paul W King, Michael W W Adams & John W Peters

  • Posted in Nat Chem Biol, Publications
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CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters

April 10th, 2017 by Mingzi M Zhang

Nature Chemical Biology 13, 607 (2017). doi:10.1038/nchembio.2341

Authors: Mingzi M Zhang, Fong Tian Wong, Yajie Wang, Shangwen Luo, Yee Hwee Lim, Elena Heng, Wan Lin Yeo, Ryan E Cobb, Behnam Enghiad, Ee Lui Ang & Huimin Zhao

Here we report an efficient CRISPR–Cas9 knock-in strategy to activate silent biosynthetic gene clusters (BGCs) in streptomycetes. We applied this one-step strategy to activate multiple BGCs of different classes in five Streptomyces species and triggered the production of unique metabolites, including a novel pentangular type II polyketide in Streptomyces viridochromogenes. This potentially scalable strategy complements existing activation approaches and facilitates discovery efforts to uncover new compounds with interesting bioactivities.

  • Posted in Nat Chem Biol, Publications
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