Measurement of In Vivo Protein Binding Affinities in a Signaling Network with Mass Spectrometry

April 5th, 2017 by Mumun Gencoglu, Alexander Schmidt and Attila Becskei

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.6b00282

Phosphorylated glycosphingolipids essential for cholesterol mobilization in Caenorhabditis elegans

April 3rd, 2017 by Sebastian Boland

Nature Chemical Biology 13, 647 (2017). doi:10.1038/nchembio.2347

Authors: Sebastian Boland, Ulrike Schmidt, Vyacheslav Zagoriy, Julio L Sampaio, Raphael F Fritsche, Regina Czerwonka, Tilo Lübken, Jakob Reimann, Sider Penkov, Hans-Joachim Knölker & Teymuras V Kurzchalia

  • Posted in Nat Chem Biol, Publications
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AtaT blocks translation initiation by N-acetylation of the initiator tRNAfMet

April 3rd, 2017 by Dukas Jurėnas

Nature Chemical Biology 13, 640 (2017). doi:10.1038/nchembio.2346

Authors: Dukas Jurėnas, Sneha Chatterjee, Albert Konijnenberg, Frank Sobott, Louis Droogmans, Abel Garcia-Pino & Laurence Van Melderen

  • Posted in Nat Chem Biol, Publications
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A Glucose-Sensing Toggle Switch for Autonomous, High Productivity Genetic Control

March 30th, 2017 by William Bothfeld, Grace Kapov and Keith E. J. Tyo

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.6b00257

The Role of SufS Is Restricted to Fe–S Cluster Biosynthesis in Escherichia coli

March 30th, 2017 by Martin Bühning, Angelo Valleriani and Silke Leimkühler

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Biochemistry
DOI: 10.1021/acs.biochem.7b00040

Simultaneous Measurement of Glucose-6-phosphate 3-Dehydrogenase (NtdC) Catalysis and the Nonenzymatic Reaction of Its Product: Kinetics and Isotope Effects on the First Step in Kanosamine Biosynthesis

March 29th, 2017 by Natasha D. Vetter and David R. J. Palmer

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Biochemistry
DOI: 10.1021/acs.biochem.7b00079

Insights into activity and inhibition from the crystal structure of human O-GlcNAcase

March 27th, 2017 by Nathaniel L Elsen

Nature Chemical Biology 13, 613 (2017). doi:10.1038/nchembio.2357

Authors: Nathaniel L Elsen, Sangita B Patel, Rachael E Ford, Dawn L Hall, Fred Hess, Hari Kandula, Maria Kornienko, John Reid, Harold Selnick, Jennifer M Shipman, Sujata Sharma, Kevin J Lumb, Stephen M Soisson & Daniel J Klein

O-GlcNAc hydrolase (OGA) catalyzes removal of βα-linked N-acetyl-D-glucosamine from serine and threonine residues. We report crystal structures of Homo sapiens OGA catalytic domain in apo and inhibited states, revealing a flexible dimer that displays three unique conformations and is characterized by subdomain α-helix swapping. These results identify new structural features of the substrate-binding groove adjacent to the catalytic site and open new opportunities for structural, mechanistic and drug discovery activities.

  • Posted in Nat Chem Biol, Publications
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Diabetes reversal by inhibition of the low-molecular-weight tyrosine phosphatase

March 27th, 2017 by Stephanie M Stanford

Nature Chemical Biology 13, 624 (2017). doi:10.1038/nchembio.2344

Authors: Stephanie M Stanford, Alexander E Aleshin, Vida Zhang, Robert J Ardecky, Michael P Hedrick, Jiwen Zou, Santhi R Ganji, Matthew R Bliss, Fusayo Yamamoto, Andrey A Bobkov, Janna Kiselar, Yingge Liu, Gregory W Cadwell, Shilpi Khare, Jinghua Yu, Antonio Barquilla, Thomas D Y Chung, Tomas Mustelin, Simon Schenk, Laurie A Bankston, Robert C Liddington, Anthony B Pinkerton & Nunzio Bottini

  • Posted in Nat Chem Biol, Publications
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Chemical screening identifies ATM as a target for alleviating senescence

March 27th, 2017 by Hyun Tae Kang

Nature Chemical Biology 13, 616 (2017). doi:10.1038/nchembio.2342

Authors: Hyun Tae Kang, Joon Tae Park, Kobong Choi, Yongsub Kim, Hyo Jei Claudia Choi, Chul Won Jung, Young-Sam Lee & Sang Chul Park

  • Posted in Nat Chem Biol, Publications
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Near-infrared optogenetic pair for protein regulation and spectral multiplexing

March 27th, 2017 by Taras A Redchuk

Nature Chemical Biology 13, 633 (2017). doi:10.1038/nchembio.2343

Authors: Taras A Redchuk, Evgeniya S Omelina, Konstantin G Chernov & Vladislav V Verkhusha

  • Posted in Nat Chem Biol, Publications
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