Ultrasensitive optical imaging with lanthanide lumiphores

November 6th, 2017 by Ukrae Cho

Ultrasensitive optical imaging with lanthanide lumiphores

Ultrasensitive optical imaging with lanthanide lumiphores, Published online: 06 November 2017; doi:10.1038/nchembio.2513

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Ultrasensitive imaging of lanthanide chelates is achieved by integrating transreflected illumination, luminescence resonance energy transfer, and time-resolved microscopy.

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Purinyl-cobamide is a native prosthetic group of reductive dehalogenases

November 6th, 2017 by Jun Yan

Purinyl-cobamide is a native prosthetic group of reductive dehalogenases

Purinyl-cobamide is a native prosthetic group of reductive dehalogenases, Published online: 06 November 2017; doi:10.1038/nchembio.2512

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In organohalide-respiring bacteria, reductive dehalogenases cleave carbon–halogen bonds using cobamide prosthetic groups. Desulfitobacterium hafniense uses an unprecedented cobamide variant, which includes unsubstituted purine, for this function.

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  • Posted in Nat Chem Biol, Publications
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Biosynthesis of an Opine Metallophore by Pseudomonas aeruginosa

November 3rd, 2017 by Jeffrey S. McFarlane and Audrey L. Lamb

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Biochemistry
DOI: 10.1021/acs.biochem.7b00804

Large Scale Mutational and Kinetic Analysis of a Self-Hydrolyzing Deoxyribozyme

November 3rd, 2017 by V. Dhamodharan, Shungo Kobori and Yohei Yokobayashi

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ACS Chemical Biology
DOI: 10.1021/acschembio.7b00621

Synthetic beta cells for fusion-mediated dynamic insulin secretion

October 30th, 2017 by Zhaowei Chen

Synthetic beta cells for fusion-mediated dynamic insulin secretion

Synthetic beta cells for fusion-mediated dynamic insulin secretion, Published online: 30 October 2017; doi:10.1038/nchembio.2511

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Synthetic beta cells were fabricated through 'vesicles-in-vesicle' liposomal superstructures equipped with glucose-sensing and membrane-fusion machinery, thus enabling sensing of graded glucose levels and secretion of insulin via fusion processes.

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PatB1 is an <i>O</i>-acetyltransferase that decorates secondary cell wall polysaccharides

October 30th, 2017 by David Sychantha

PatB1 is an O-acetyltransferase that decorates secondary cell wall polysaccharides

PatB1 is an <i>O</i>-acetyltransferase that decorates secondary cell wall polysaccharides, Published online: 30 October 2017; doi:10.1038/nchembio.2509

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A chemically synthesized analog of Bacillus cereus secondary cell wall polysaccharide (SCWP) facilitates the identification of PatB1 as a SCWP O-acetyltransferase, and the structure of PatB1 provides insights into its catalytic mechanism.

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Small molecule promotes β-catenin citrullination and inhibits Wnt signaling in cancer

October 30th, 2017 by Yi Qu

Small molecule promotes β-catenin citrullination and inhibits Wnt signaling in cancer

Small molecule promotes β-catenin citrullination and inhibits Wnt signaling in cancer, Published online: 30 October 2017; doi:10.1038/nchembio.2510

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The small molecule nitazoxanide (NTZ) was identified as a Wnt inhibitor by promoting protein citrullination of β-catenin through increased cytosolic calcium and PAD2 protein stabilization. β-catenin citrullination results in proteasomal degradation.

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The Conformational Change in Elongation Factor Tu Involves Separation of Its Domains

October 27th, 2017 by Jonathan Lai, Zhaleh Ghaemi and Zaida Luthey-Schulten

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Biochemistry
DOI: 10.1021/acs.biochem.7b00591

Correction to Probing the Binding Mechanism of Mnk Inhibitors by Docking and Molecular Dynamics Simulations

October 27th, 2017 by Srinivasaraghavan Kannan, Anders Poulsen, Hai Yan Yang, Melvyn Ho, Shi Hua Ang, Tan Sum Wai Eldwin, Duraiswamy Athisayamani Jeyaraj, Lohitha Rao Chennamaneni, Boping Liu, Jeffrey Hill, Chandra S. Verma and Kassoum Nacro

Biochemistry
DOI: 10.1021/acs.biochem.7b00996

Structural and Functional Effects of Cytochrome b5 Interactions with Human Cytochrome P450 Enzymes [Enzymology]

October 27th, 2017 by Aaron G. Bart, Emily E. Scott

The small heme-containing protein cytochrome b5 can facilitate, inhibit, or have no effect on cytochrome P450 catalysis, often in a P450-dependent and substrate-dependent manner that is not well understood. Herein solution NMR was used to identify b5 residues interacting with different human drug-metabolizing P450 enzymes. NMR results revealed that P450 enzymes bound to either b5 α4-5 (CYP2A6 and CYP2E1) or this region and α2-3 (CYP2D6 and CYP3A4) and suggested variation in the affinity for b5. Mutations of key b5 residues suggest that not only are different b5 surfaces responsible for binding different P450 enzymes, but that these different complexes are relevant to the observed effects on P450 catalysis.