Metabolic engineering of a carbapenem antibiotic synthesis pathway in <i>Escherichia coli</i>

June 25th, 2018 by Helena Shomar

Metabolic engineering of a carbapenem antibiotic synthesis pathway in Escherichia coli

Metabolic engineering of a carbapenem antibiotic synthesis pathway in <i>Escherichia coli</i>, Published online: 25 June 2018; doi:10.1038/s41589-018-0084-6

Efficient production of a simple carbapenem antibiotic in Escherichia coli is achieved by a combination of feedback-resistant enzymes for increased precursor biosynthesis and inhibition of fatty acid synthesis for tolerance toward the toxic product.
  • Posted in Nat Chem Biol, Publications
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Cleavage of a carbon–fluorine bond by an engineered cysteine dioxygenase

June 25th, 2018 by Jiasong Li

Cleavage of a carbon–fluorine bond by an engineered cysteine dioxygenase

Cleavage of a carbon–fluorine bond by an engineered cysteine dioxygenase, Published online: 25 June 2018; doi:10.1038/s41589-018-0085-5

Engineered variants of cysteine dioxygenase containing a halogen-substituted tyrosine analog provide insights into the process of Cys–Tyr cross-link formation and indicate that the enzyme can catalyze oxidative cleavage of a carbon–fluorine bond.
  • Posted in Nat Chem Biol, Publications
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Kinome rewiring reveals AURKA limits PI3K-pathway inhibitor efficacy in breast cancer

June 25th, 2018 by Hayley J. Donnella

Kinome rewiring reveals AURKA limits PI3K-pathway inhibitor efficacy in breast cancer

Kinome rewiring reveals AURKA limits PI3K-pathway inhibitor efficacy in breast cancer, Published online: 25 June 2018; doi:10.1038/s41589-018-0081-9

Proteomic mapping of dynamic changes in kinase signaling after drug treatment identifies that AURKA inhibition is required for drug sensitivity, representing a new co-targeting opportunity with PI3K, AKT, or mTOR inhibitors in breast cancer.
  • Posted in Nat Chem Biol, Publications
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Unraveling the rewired network

June 25th, 2018 by Vinayak Palve

Unraveling the rewired network

Unraveling the rewired network, Published online: 25 June 2018; doi:10.1038/s41589-018-0083-7

Adaptive survival signaling can promote resistance to individual kinase inhibitors. A new study used a pathway-based approach to characterize shared kinome-wide rewiring mechanisms across multiple kinase inhibitors and developed rational drug-combination approaches that target cross-talk between two signaling pathways.

[ASAP] A Bifunctional Noncanonical Amino Acid: Synthesis, Expression, and Residue-Specific Proteome-wide Incorporation

June 21st, 2018 by Jan-Erik Hoffmann, Dmytro Dziuba, Frank Stein, Carsten Schultz

TOC Graphic

Biochemistry
DOI: 10.1021/acs.biochem.8b00397

[ASAP] So Close, yet Sulfur Away: Opening the Nitrogenase Cofactor Structure Creates a Binding Site

June 20th, 2018 by Kazimer L. Skubi, Patrick L. Holland

TOC Graphic

Biochemistry
DOI: 10.1021/acs.biochem.8b00529

Please hold for chaperones

June 18th, 2018 by Caitlin Deane

Please hold for chaperones

Please hold for chaperones, Published online: 18 June 2018; doi:10.1038/s41589-018-0092-6

Please hold for chaperones

Tricks for taking up residence

June 18th, 2018 by Mirella Bucci

Tricks for taking up residence

Tricks for taking up residence, Published online: 18 June 2018; doi:10.1038/s41589-018-0094-4

Tricks for taking up residence

Stabilizing synapsis

June 18th, 2018 by Yiyun Song

Stabilizing synapsis

Stabilizing synapsis, Published online: 18 June 2018; doi:10.1038/s41589-018-0095-3

Stabilizing synapsis

Single-molecule probing of the conformational homogeneity of the ABC transporter BtuCD

June 18th, 2018 by Min Yang

Single-molecule probing of the conformational homogeneity of the ABC transporter BtuCD

Single-molecule probing of the conformational homogeneity of the ABC transporter BtuCD, Published online: 18 June 2018; doi:10.1038/s41589-018-0088-2

A single-molecule approach combined with molecular dynamics simulations to examine the conformational dynamics of the Escherichia coli ABC transporter BtuCD defines a coordinated sequence of events leading to unidirectional transport.
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