Enabling Graded and Large-Scale Multiplex of Desired Genes Using a Dual-Mode dCas9 Activator in Saccharomyces cerevisiae

July 27th, 2017 by Matthew Deaner, Julio Mejia and Hal S. Alper

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.7b00163
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Palmitoylation is a prerequisite for dimerization-dependent raftophilicity of rhodopsin [Membrane Biology]

July 26th, 2017 by Keiji Seno, Fumio Hayashi

The visual photopigment rhodopsin (Rh) is a prototypical G protein-coupled receptor (GPCR) responsible for initiation of the phototransduction cascade in rod photoreceptors. Similar to other GPCRs, Rh can form dimers or even higher oligomers, and tends to have a supramolecular organization that is likely important in the dim light response. Rh also exhibits high affinity for lipid rafts (raftophilicity) upon light-dependent binding with cognate G protein transducin (Gt), suggesting the presence of lipid raft-like domains in the retinal disk membrane and their importance in phototransduction. However, the relationship between Rh oligomerization and lipid rafts in the disk membrane remains to be explored. Given previous findings that Gt binds to dimeric Rh and Rh is post-translationally modified with two highly raftophilic palmitoyl moieties, we hypothesized that Rh becomes raftophilic upon dimerization. Here, we tested this hypothesis biochemically. First, we found that Rh*-Gt complexes in the detergent-resistant membrane (DRM) are partially resistant to cholesterol depletion by methyl-β-cyclodextrin (MCD), and the stoichiometry of Rh to Gt in this MCD-resistant complex is 2:1. We then found that IgG-crosslinking renders Rh highly raftophilic, supporting the premise that Rh becomes raftophilic upon dimerization. Depalmitoylation of Rh with the reduction of thioester linkages by dithiothreitol blocked the translocation of IgG-crosslinked Rh to the DRM, highlighting the importance of the two palmitoyl moieties in the dimerization-dependent raftophilicity of Rh. These results indicate that palmitoylated GPCRs, such as Rh, may acquire raftophilicity upon G protein-stabilized dimerization and thereby organize receptor-cluster rafts by recruiting raftophilic lipids.

Unique Thermal Stability of Unnatural Hydrophobic Ds Bases in Double-Stranded DNAs

July 26th, 2017 by Michiko Kimoto and Ichiro Hirao

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.7b00165

Copper import in Escherichia coli by the yersiniabactin metallophore system

July 24th, 2017 by Eun-Ik Koh

Nature Chemical Biology 13, 1016 (2017). doi:10.1038/nchembio.2441

Authors: Eun-Ik Koh, Anne E Robinson, Nilantha Bandara, Buck E Rogers & Jeffrey P Henderson

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FRET monitoring of a nonribosomal peptide synthetase

July 24th, 2017 by Jonas Alfermann

Nature Chemical Biology 13, 1009 (2017). doi:10.1038/nchembio.2435

Authors: Jonas Alfermann, Xun Sun, Florian Mayerthaler, Thomas E Morrell, Eva Dehling, Gerrit Volkmann, Tamiki Komatsuzaki, Haw Yang & Henning D Mootz

Toxicity and repair of DNA adducts produced by the natural product yatakemycin

July 24th, 2017 by Elwood A Mullins

Nature Chemical Biology 13, 1002 (2017). doi:10.1038/nchembio.2439

Authors: Elwood A Mullins, Rongxin Shi & Brandt F Eichman

  • Posted in Nat Chem Biol, Publications
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Peptidomimetic inhibitors of APC–Asef interaction block colorectal cancer migration

July 24th, 2017 by Haiming Jiang

Nature Chemical Biology 13, 994 (2017). doi:10.1038/nchembio.2442

Authors: Haiming Jiang, Rong Deng, Xiuyan Yang, Jialin Shang, Shaoyong Lu, Yanlong Zhao, Kun Song, Xinyi Liu, Qiufen Zhang, Yu Chen, Y Eugene Chinn, Geng Wu, Jian Li, Guoqiang Chen, Jianxiu Yu & Jian Zhang

  • Posted in Nat Chem Biol, Publications
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Functional annotation of chemical libraries across diverse biological processes

July 24th, 2017 by Jeff S Piotrowski

Nature Chemical Biology 13, 982 (2017). doi:10.1038/nchembio.2436

Authors: Jeff S Piotrowski, Sheena C Li, Raamesh Deshpande, Scott W Simpkins, Justin Nelson, Yoko Yashiroda, Jacqueline M Barber, Hamid Safizadeh, Erin Wilson, Hiroki Okada, Abraham A Gebre, Karen Kubo, Nikko P Torres, Marissa A LeBlanc, Kerry Andrusiak, Reika Okamoto, Mami Yoshimura, Eva DeRango-Adem, Jolanda van Leeuwen, Katsuhiko Shirahige, Anastasia Baryshnikova, Grant W Brown, Hiroyuki Hirano, Michael Costanzo, Brenda Andrews, Yoshikazu Ohya, Hiroyuki Osada, Minoru Yoshida, Chad L Myers & Charles Boone

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Streptomyces virginiae PPDC Is a New Type of Phenylpyruvate Decarboxylase Composed of Two Subunits

July 18th, 2017 by Xiaoshu Xu, Chao Wang, Jun Chen and Sheng Yang

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ACS Chemical Biology
DOI: 10.1021/acschembio.7b00307
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Biocatalysis: Achieving aminase activity

July 18th, 2017 by Caitlin Deane

Nature Chemical Biology 13, 817 (2017). doi:10.1038/nchembio.2447

Author: Caitlin Deane