TAGing for destruction

March 26th, 2018 by Aisha Yesbolatova

TAGing for destruction

TAGing for destruction, Published online: 26 March 2018; doi:10.1038/s41589-018-0024-5

Proteins brought to an E3 ubiquitin ligase are polyubiquitylated for rapid degradation via the proteasome. A new study developed a tag-based degradation system using heterobifunctional degradation inducers called dTAGs, which rapidly depleted FKBP12F36V-fused proteins in cultured cells and in mice.

[ASAP] Structures of the Catalytic Domain of Bacterial Primase DnaG in Complexes with DNA Provide Insight into Key Priming Events

March 22nd, 2018 by Caixia Hou, Tapan Biswas, Oleg V. Tsodikov

TOC Graphic

Biochemistry
DOI: 10.1021/acs.biochem.8b00036

[ASAP] A Complementary Chemical and Genomic Screening Approach for Druggable Targets in the Nrf2 Pathway and Small Molecule Inhibitors to Overcome Cancer Cell Drug Resistance

March 21st, 2018 by James H. Matthews, Xiao Liang, Valerie J. Paul, Hendrik Luesch

TOC Graphic

ACS Chemical Biology
DOI: 10.1021/acschembio.7b01025
  • Posted in ACS Chemical Biology, Publications
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Whole-organism phenotypic screening for anti-infectives promoting host health

March 19th, 2018 by Anne E. Clatworthy

Whole-organism phenotypic screening for anti-infectives promoting host health

Whole-organism phenotypic screening for anti-infectives promoting host health, Published online: 19 March 2018; doi:10.1038/s41589-018-0018-3

Targeting the host during antibiotic discovery efforts is a viable strategy, and the approach has benefited from phenotypic screening of model organisms such as worms, zebrafish, and mice.
  • Posted in Nat Chem Biol, Publications
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Structural basis for backbone N-methylation by an interrupted adenylation domain

March 19th, 2018 by Shogo Mori

Structural basis for backbone N-methylation by an interrupted adenylation domain

Structural basis for backbone N-methylation by an interrupted adenylation domain, Published online: 19 March 2018; doi:10.1038/s41589-018-0014-7

The crystal structure of a methyltransferase domain embedded within an interrupted adenylation domain provides insight into how a nonribosomal peptide synthetase N-methylates amino acid precursors for their incorporation into the peptide product.
  • Posted in Nat Chem Biol, Publications
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Mix and match

March 19th, 2018 by Karin Kuehnel

Mix and match

Mix and match, Published online: 19 March 2018; doi:10.1038/s41589-018-0028-1

Mix and match

Discovery of enzymes for toluene synthesis from anoxic microbial communities

March 19th, 2018 by Harry R. Beller

Discovery of enzymes for toluene synthesis from anoxic microbial communities

Discovery of enzymes for toluene synthesis from anoxic microbial communities, Published online: 19 March 2018; doi:10.1038/s41589-018-0017-4

The source of biological toluene production in diverse anoxic microbial communities is a glycyl radical enzyme that catalyzes phenylacetate decarboxylation (PhdB), and its cognate activating radical S-adenosylmethionine enzyme (PhdA).
  • Posted in Nat Chem Biol, Publications
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A ‘cis’ted localization

March 19th, 2018 by Mirella Bucci

A ‘cis’ted localization

A ‘cis’ted localization, Published online: 19 March 2018; doi:10.1038/s41589-018-0029-0

A ‘cis’ted localization

RaPID hookup

March 19th, 2018 by Grant Miura

RaPID hookup

RaPID hookup, Published online: 19 March 2018; doi:10.1038/s41589-018-0030-7

RaPID hookup

An optically controlled probe identifies lipid-gating fenestrations within the TRPC3 channel

March 19th, 2018 by Michaela Lichtenegger

An optically controlled probe identifies lipid-gating fenestrations within the TRPC3 channel

An optically controlled probe identifies lipid-gating fenestrations within the TRPC3 channel, Published online: 19 March 2018; doi:10.1038/s41589-018-0015-6

A photoswitchable diacylglycerol enabled a screen that found critical TRPC3 lipid-sensing residues and identified a lateral fenestration in the pore domain that allows lipids to protrude into the permeation pathway to control channel gating.
  • Posted in Nat Chem Biol, Publications
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