Metal homeostasis: Pumping iron

June 20th, 2017 by Grant Miura

Nature Chemical Biology 13, 693 (2017). doi:10.1038/nchembio.2423

Author: Grant Miura

Antimicrobials: Targeting fungal chromatin

June 20th, 2017 by Stéphane Larochelle

Nature Chemical Biology 13, 693 (2017). doi:10.1038/nchembio.2424

Author: Stéphane Larochelle

Circadian regulation: Switching periods

June 20th, 2017 by Grant Miura

Nature Chemical Biology 13, 693 (2017). doi:10.1038/nchembio.2426

Author: Grant Miura

Synthetic biology: Playing favorites

June 20th, 2017 by Ross Cloney

Nature Chemical Biology 13, 693 (2017). doi:10.1038/nchembio.2425

Author: Ross Cloney

Peptidoglycan: Another brick in the wall

June 20th, 2017 by Anthony J Clarke

Nature Chemical Biology 13, 695 (2017). doi:10.1038/nchembio.2419

Author: Anthony J Clarke

Lipid II embodies the bricks used to build the essential bacterial cell wall component peptidoglycan. A facile new procedure for preparation of species-specific Lipid II in high yields can now be used to unlock the door to antibiotic discovery.

A heme-dependent enzyme forms the nitrogen–nitrogen bond in piperazate

June 19th, 2017 by Yi-Ling Du

Nature Chemical Biology 13, 836 (2017). doi:10.1038/nchembio.2411

Authors: Yi-Ling Du, Hai-Yan He, Melanie A Higgins & Katherine S Ryan

Molecules containing a nitrogen–nitrogen (N–N) linkage have a variety of structures and biological activities; however, no enzyme has yet been demonstrated to catalyze N–N bond formation in an organic molecule. Here we report that the heme-dependent enzyme KtzT from Kutzneria sp. 744 catalyzes N–N bond formation in the biosynthesis of piperazate, a building block for nonribosomal peptides.

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A scaffold protein that chaperones a cysteine-sulfenic acid in H2O2 signaling

June 19th, 2017 by Antoine Bersweiler

Nature Chemical Biology 13, 909 (2017). doi:10.1038/nchembio.2412

Authors: Antoine Bersweiler, Benoît D'Autréaux, Hortense Mazon, Alexandre Kriznik, Gemma Belli, Agnès Delaunay-Moisan, Michel B Toledano & Sophie Rahuel-Clermont

  • Posted in Nat Chem Biol, Publications
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A water-soluble DsbB variant that catalyzes disulfide-bond formation in vivo

June 19th, 2017 by Dario Mizrachi

Nature Chemical Biology 13, 1022 (2017). doi:10.1038/nchembio.2409

Authors: Dario Mizrachi, Michael-Paul Robinson, Guoping Ren, Na Ke, Mehmet Berkmen & Matthew P DeLisa

  • Posted in Nat Chem Biol, Publications
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Nucleation and growth of a bacterial functional amyloid at single-fiber resolution

June 19th, 2017 by Mike Sleutel

Nature Chemical Biology 13, 902 (2017). doi:10.1038/nchembio.2413

Authors: Mike Sleutel, Imke Van den Broeck, Nani Van Gerven, Cécile Feuillie, Wim Jonckheere, Claire Valotteau, Yves F Dufrêne & Han Remaut

  • Posted in Nat Chem Biol, Publications
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Cpf1 proteins excise CRISPR RNAs from mRNA transcripts in mammalian cells

June 19th, 2017 by Guocai Zhong

Nature Chemical Biology 13, 839 (2017). doi:10.1038/nchembio.2410

Authors: Guocai Zhong, Haimin Wang, Yujun Li, Mai H Tran & Michael Farzan

Cpf1 is a CRISPR effector protein that has greater specificity than Streptococcus pyogenes Cas9 (SpCas9) in genome-editing applications. Here we show that Lachnospiraceae bacterium (Lb) and Acidaminococus sp. (As) Cpf1 orthologs have RNase activities that can excise multiple CRISPR RNAs (crRNAs) from a single RNA polymerase II–driven RNA transcript expressed in mammalian cells. This property simplifies modification of multiple genomic targets and can be used to increase the efficiency of Cpf1-mediated editing.

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