Bisphosphoglycerate mutase controls serine pathway flux via 3-phosphoglycerate

August 7th, 2017 by Rob C Oslund

Nature Chemical Biology 13, 1081 (2017). doi:10.1038/nchembio.2453

Authors: Rob C Oslund, Xiaoyang Su, Michael Haugbro, Jung-Min Kee, Mark Esposito, Yael David, Boyuan Wang, Eva Ge, David H Perlman, Yibin Kang, Tom W Muir & Joshua D Rabinowitz

  • Posted in Nat Chem Biol, Publications
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A genetically encoded tool for manipulation of NADP+/NADPH in living cells

August 7th, 2017 by Valentin Cracan

Nature Chemical Biology 13, 1088 (2017). doi:10.1038/nchembio.2454

Authors: Valentin Cracan, Denis V Titov, Hongying Shen, Zenon Grabarek & Vamsi K Mootha

  • Posted in Nat Chem Biol, Publications
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Molecular basis for the unusual ring reconstruction in fungal meroterpenoid biogenesis

July 31st, 2017 by Takahiro Mori

Nature Chemical Biology 13, 1066 (2017). doi:10.1038/nchembio.2443

Authors: Takahiro Mori, Taiki Iwabuchi, Shotaro Hoshino, Hang Wang, Yudai Matsuda & Ikuro Abe

  • Posted in Nat Chem Biol, Publications
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Modulating the DNA polymerase β reaction equilibrium to dissect the reverse reaction

July 31st, 2017 by David D Shock

Nature Chemical Biology 13, 1074 (2017). doi:10.1038/nchembio.2450

Authors: David D Shock, Bret D Freudenthal, William A Beard & Samuel H Wilson

  • Posted in Nat Chem Biol, Publications
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Enabling Graded and Large-Scale Multiplex of Desired Genes Using a Dual-Mode dCas9 Activator in Saccharomyces cerevisiae

July 27th, 2017 by Matthew Deaner, Julio Mejia and Hal S. Alper

TOC Graphic

ACS Synthetic Biology
DOI: 10.1021/acssynbio.7b00163
  • Posted in ACS Synthetic Biology, Publications
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Palmitoylation is a prerequisite for dimerization-dependent raftophilicity of rhodopsin [Membrane Biology]

July 26th, 2017 by Keiji Seno, Fumio Hayashi

The visual photopigment rhodopsin (Rh) is a prototypical G protein-coupled receptor (GPCR) responsible for initiation of the phototransduction cascade in rod photoreceptors. Similar to other GPCRs, Rh can form dimers or even higher oligomers, and tends to have a supramolecular organization that is likely important in the dim light response. Rh also exhibits high affinity for lipid rafts (raftophilicity) upon light-dependent binding with cognate G protein transducin (Gt), suggesting the presence of lipid raft-like domains in the retinal disk membrane and their importance in phototransduction. However, the relationship between Rh oligomerization and lipid rafts in the disk membrane remains to be explored. Given previous findings that Gt binds to dimeric Rh and Rh is post-translationally modified with two highly raftophilic palmitoyl moieties, we hypothesized that Rh becomes raftophilic upon dimerization. Here, we tested this hypothesis biochemically. First, we found that Rh*-Gt complexes in the detergent-resistant membrane (DRM) are partially resistant to cholesterol depletion by methyl-β-cyclodextrin (MCD), and the stoichiometry of Rh to Gt in this MCD-resistant complex is 2:1. We then found that IgG-crosslinking renders Rh highly raftophilic, supporting the premise that Rh becomes raftophilic upon dimerization. Depalmitoylation of Rh with the reduction of thioester linkages by dithiothreitol blocked the translocation of IgG-crosslinked Rh to the DRM, highlighting the importance of the two palmitoyl moieties in the dimerization-dependent raftophilicity of Rh. These results indicate that palmitoylated GPCRs, such as Rh, may acquire raftophilicity upon G protein-stabilized dimerization and thereby organize receptor-cluster rafts by recruiting raftophilic lipids.

Unique Thermal Stability of Unnatural Hydrophobic Ds Bases in Double-Stranded DNAs

July 26th, 2017 by Michiko Kimoto and Ichiro Hirao

TOC Graphic

ACS Synthetic Biology
DOI: 10.1021/acssynbio.7b00165

Copper import in Escherichia coli by the yersiniabactin metallophore system

July 24th, 2017 by Eun-Ik Koh

Nature Chemical Biology 13, 1016 (2017). doi:10.1038/nchembio.2441

Authors: Eun-Ik Koh, Anne E Robinson, Nilantha Bandara, Buck E Rogers & Jeffrey P Henderson

  • Posted in Nat Chem Biol, Publications
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FRET monitoring of a nonribosomal peptide synthetase

July 24th, 2017 by Jonas Alfermann

Nature Chemical Biology 13, 1009 (2017). doi:10.1038/nchembio.2435

Authors: Jonas Alfermann, Xun Sun, Florian Mayerthaler, Thomas E Morrell, Eva Dehling, Gerrit Volkmann, Tamiki Komatsuzaki, Haw Yang & Henning D Mootz

Toxicity and repair of DNA adducts produced by the natural product yatakemycin

July 24th, 2017 by Elwood A Mullins

Nature Chemical Biology 13, 1002 (2017). doi:10.1038/nchembio.2439

Authors: Elwood A Mullins, Rongxin Shi & Brandt F Eichman

  • Posted in Nat Chem Biol, Publications
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