A Molecular Perspective of Mammalian Autophagosome Biogenesis [Membrane Biology]

January 25th, 2018 by Thomas J. Mercer, Andrea Gubas, Sharon A. Tooze

Autophagy is a highly conserved process, essential for the maintenance of cellular homeostasis. Autophagy occurs at a basal level in all cells, but can be upregulated during stress, starvation, or infection. Misregulation of autophagy has been linked to various disorders, including cancer, neurodegeneration and immune diseases. Here, we discuss the essential proteins acting in the formation of an autophagosome, with a focus on the ULK and VPS34 kinase complexes, PI3P effector proteins and the transmembrane autophagy-related protein ATG9. The function and regulation of these and other autophagy-related proteins, acting during formation will be addressed, in particular, during amino acid starvation.

Analyzing and Tuning Ribozyme Activity by Deep Sequencing To Modulate Gene Expression Level in Mammalian Cells

January 24th, 2018 by Shungo Kobori and Yohei Yokobayashi

TOC Graphic

ACS Synthetic Biology
DOI: 10.1021/acssynbio.7b00367
  • Posted in ACS Synthetic Biology, Publications
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A whole-animal platform to advance a clinical kinase inhibitor into new disease space

January 22nd, 2018 by Masahiro Sonoshita

A whole-animal platform to advance a clinical kinase inhibitor into new disease space

A whole-animal platform to advance a clinical kinase inhibitor into new disease space, Published online: 22 January 2018; doi:10.1038/nchembio.2556

Combining Drosophila genetics with chemistry and computation led to the development of novel kinase inhibitors based on the RAF-targeting drug sorafenib that reveal the RET oncogene as an enhancer of drug action and improve the therapeutic window in medullary thyroid carcinoma models.
  • Posted in Nat Chem Biol, Publications
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Chemical hijacking of auxin signaling with an engineered auxin–TIR1 pair

January 22nd, 2018 by Naoyuki Uchida

Chemical hijacking of auxin signaling with an engineered auxin–TIR1 pair

Chemical hijacking of auxin signaling with an engineered auxin–TIR1 pair, Published online: 22 January 2018; doi:10.1038/nchembio.2555

A synthetic, orthogonal pair of the plant hormone auxin and its receptor TIR1 was engineered to hijack auxin signaling without interfering with the endogenous system. The synthetic system conclusively demonstrates the role for TIR1 in auxin-induced acid growth.
  • Posted in Nat Chem Biol, Publications
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MinGenome: An In Silico Top-Down Approach for the Synthesis of Minimized Genomes

January 20th, 2018 by Lin Wang and Costas D. Maranas

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.7b00296

Toward an orthogonal central dogma

January 16th, 2018 by Chang C Liu

Toward an orthogonal central dogma

Toward an orthogonal central dogma, Published online: 16 January 2018; doi:10.1038/nchembio.2554

The central dogma processes of DNA replication, transcription, and translation are responsible for the maintenance and expression of every gene in an organism. An orthogonal central dogma may insulate genetic programs from host regulation and allow expansion of the roles of these processes within the cell.

Cell biology: Eaten up from the inside

January 16th, 2018 by Karin Kuehnel

Cell biology: Eaten up from the inside

Cell biology: Eaten up from the inside, Published online: 16 January 2018; doi:10.1038/nchembio.2563

Cell biology: Eaten up from the inside

Plant hormones: Metabolic end run to jasmonate

January 16th, 2018 by Gregg A Howe

Plant hormones: Metabolic end run to jasmonate

Plant hormones: Metabolic end run to jasmonate, Published online: 16 January 2018; doi:10.1038/nchembio.2553

The lipid-derived hormone jasmonate promotes durable resistance of plants to a myriad of herbivores and pathogens. New evidence reveals an alternative pathway for the terminal steps of jasmonate biosynthesis and further advances our understanding of bioactive oxylipins in the plant kingdom.

RNA Splicing: Making the cut

January 16th, 2018 by Stéphane Larochelle

RNA Splicing: Making the cut

RNA Splicing: Making the cut, Published online: 16 January 2018; doi:10.1038/nchembio.2564

RNA Splicing: Making the cut

Drug development: Allosteric inhibitors hit USP7 hard

January 16th, 2018 by Wei Zhang

Drug development: Allosteric inhibitors hit USP7 hard

Drug development: Allosteric inhibitors hit USP7 hard, Published online: 16 January 2018; doi:10.1038/nchembio.2557

A potent and specific small-molecule inhibitor of a long-sought-after anticancer drug target, USP7, that acts allosterically to inhibit MDM2-stabilizing activity foretells of more allosteric inhibitors for deubiquitinases and E3 ligases.