Trp-ing upon new repressors

February 26th, 2018 by Andreas K. Brödel

Trp-ing upon new repressors

Trp-ing upon new repressors, Published online: 26 February 2018; doi:10.1038/s41589-018-0012-9

Bioengineers have used directed evolution to generate a new family of synthetic transcription factors based on the tryptophan repressor. The evolved repressor family enables researchers to build new gene circuits for biomedical applications.

Directed evolution of a synthetic phylogeny of programmable Trp repressors

February 26th, 2018 by Jared W. Ellefson

Directed evolution of a synthetic phylogeny of programmable Trp repressors

Directed evolution of a synthetic phylogeny of programmable Trp repressors, Published online: 26 February 2018; doi:10.1038/s41589-018-0006-7

Directed evolution of Trp repressor (TrpR) variants that are responsive to halogenated tryptophan analogs and recognize new operator sites serve as useful components for constructing complex gene expression networks.
  • Posted in Nat Chem Biol, Publications
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Resistance to nonribosomal peptide antibiotics mediated by <span class=”small-caps”>d</span>-stereospecific peptidases

February 26th, 2018 by Yong-Xin Li

Resistance to nonribosomal peptide antibiotics mediated by d-stereospecific peptidases

Resistance to nonribosomal peptide antibiotics mediated by <span class="small-caps">d</span>-stereospecific peptidases, Published online: 26 February 2018; doi:10.1038/s41589-018-0009-4

Networking-associated genome mining on bacterial genomes followed by chemical and enzymatic analysis identified a mechanism of resistance toward nonribosomal peptide antibiotics based on hydrolytic cleavage by d-stereospecific peptidases.
  • Posted in Nat Chem Biol, Publications
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A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters

February 26th, 2018 by Kiryl D. Piatkevich

A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters

A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters, Published online: 26 February 2018; doi:10.1038/s41589-018-0004-9

Directed evolution of opsins via robotic high-content screening finds a fluorescent reporter of voltage that is simultaneously optimized for brightness, localization and voltage sensitivity and is applicable in three model systems.
  • Posted in Nat Chem Biol, Publications
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Sequential Protein Expression and Capsid Assembly in Cell: Toward the Study of Multiprotein Viral Capsids Using Solid-State Nuclear Magnetic Resonance Techniques

February 23rd, 2018 by Sébastien Alphonse, Boris Itin, Reza Khayat and Ranajeet Ghose

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Biochemistry
DOI: 10.1021/acs.biochem.8b00003

Protein Plasticity and Peptide Editing in the MHC I Antigen Processing Pathway

February 22nd, 2018 by Tim Elliott and Andy van Hateren

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Biochemistry
DOI: 10.1021/acs.biochem.7b01307

Orthogonal Information Encoding in Living Cells with High Error-Tolerance, Safety, and Fidelity

February 21st, 2018 by Lifu Song and An-Ping Zeng

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.7b00382

More Than a Light Switch: Engineering Unconventional Fluorescent Configurations for Biological Sensing

February 20th, 2018 by William J. Peveler and W. Russ Algar

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ACS Chemical Biology
DOI: 10.1021/acschembio.7b01022
  • Posted in ACS Chemical Biology, Publications
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Oxidative demethylation of algal carbohydrates by cytochrome P450 monooxygenases

February 19th, 2018 by Lukas Reisky

Oxidative demethylation of algal carbohydrates by cytochrome P450 monooxygenases

Oxidative demethylation of algal carbohydrates by cytochrome P450 monooxygenases, Published online: 19 February 2018; doi:10.1038/s41589-018-0005-8

The discovery of cytochrome P450 monooxygenases that catalyze oxidative demethylation of 6-O-methyl-d-galactose reveals a new activity of cytochrome P450 enzymes and their role in polysaccharide biomass degradation in marine bacteria.
  • Posted in Nat Chem Biol, Publications
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Altering form for function

February 14th, 2018 by Nature Chemical Biology - Issue - nature.com science feeds

Altering form for function

Altering form for function, Published online: 14 February 2018; doi:10.1038/nchembio.2582

Elucidating the mechanisms by which biomolecules are chemically modified and how these alterations regulate biological pathways represents a leading frontier in chemical biology.