The Conformational Change in Elongation Factor Tu Involves Separation of Its Domains

October 27th, 2017 by Jonathan Lai, Zhaleh Ghaemi and Zaida Luthey-Schulten

TOC Graphic

Biochemistry
DOI: 10.1021/acs.biochem.7b00591

Correction to Probing the Binding Mechanism of Mnk Inhibitors by Docking and Molecular Dynamics Simulations

October 27th, 2017 by Srinivasaraghavan Kannan, Anders Poulsen, Hai Yan Yang, Melvyn Ho, Shi Hua Ang, Tan Sum Wai Eldwin, Duraiswamy Athisayamani Jeyaraj, Lohitha Rao Chennamaneni, Boping Liu, Jeffrey Hill, Chandra S. Verma and Kassoum Nacro

Biochemistry
DOI: 10.1021/acs.biochem.7b00996

Structural and Functional Effects of Cytochrome b5 Interactions with Human Cytochrome P450 Enzymes [Enzymology]

October 27th, 2017 by Aaron G. Bart, Emily E. Scott

The small heme-containing protein cytochrome b5 can facilitate, inhibit, or have no effect on cytochrome P450 catalysis, often in a P450-dependent and substrate-dependent manner that is not well understood. Herein solution NMR was used to identify b5 residues interacting with different human drug-metabolizing P450 enzymes. NMR results revealed that P450 enzymes bound to either b5 α4-5 (CYP2A6 and CYP2E1) or this region and α2-3 (CYP2D6 and CYP3A4) and suggested variation in the affinity for b5. Mutations of key b5 residues suggest that not only are different b5 surfaces responsible for binding different P450 enzymes, but that these different complexes are relevant to the observed effects on P450 catalysis.

The G126V Mutation in the Mouse Prion Protein Hinders Nucleation-Dependent Fibril Formation by Slowing Initial Fibril Growth and by Increasing the Critical Concentration

October 26th, 2017 by Ambadi Thody Sabareesan and Jayant B. Udgaonkar

TOC Graphic

Biochemistry
DOI: 10.1021/acs.biochem.7b00894

Next-Generation Photodynamic Therapy: New Probes for Cancer Imaging and Treatment

October 26th, 2017 by Andrew A. Beharry

TOC Graphic

Biochemistry
DOI: 10.1021/acs.biochem.7b01037

Sequence-Based Prediction of Cysteine Reactivity Using Machine Learning

October 26th, 2017 by Haobo Wang, Xuemin Chen, Can Li, Yuan Liu, Fan Yang and Chu Wang

TOC Graphic

Biochemistry
DOI: 10.1021/acs.biochem.7b00897

CRISPRi and CRISPRa Screens in Mammalian Cells for Precision Biology and Medicine

October 24th, 2017 by Martin Kampmann

TOC Graphic

ACS Chemical Biology
DOI: 10.1021/acschembio.7b00657

Bacterial Branched-Chain Amino Acid Biosynthesis: Structures, Mechanisms, and Drugability

October 23rd, 2017 by Tathyana M. Amorim Franco and John S. Blanchard

TOC Graphic

Biochemistry
DOI: 10.1021/acs.biochem.7b00849

Electrophilic probes for deciphering substrate recognition by O-GlcNAc transferase

October 23rd, 2017 by Chia-Wei Hu

Electrophilic probes for deciphering substrate recognition by O-GlcNAc transferase

Electrophilic probes for deciphering substrate recognition by O-GlcNAc transferase, Published online: 23 October 2017; doi:10.1038/nchembio.2494

NatureArticleSnippet(type=short-summary, markup=

N-Acetylglucosamine derivatives equipped with electrophilic groups and handles for subsequent chemical tagging are useful probes of substrate recognition by O-GlcNAc transferases and enable the capture of transient protein substrates of these enzymes.

, isJats=true)
  • Posted in Nat Chem Biol, Publications
  • Comments Off on Electrophilic probes for deciphering substrate recognition by O-GlcNAc transferase

Rational design of proteins that exchange on functional timescales

October 23rd, 2017 by James A Davey

Rational design of proteins that exchange on functional timescales

Rational design of proteins that exchange on functional timescales, Published online: 23 October 2017; doi:10.1038/nchembio.2503

NatureArticleSnippet(type=short-summary, markup=

The development of a computational protein design method, meta-multistate design, enables the design and validation of protein variants termed DANCERs that spontaneously exchange between predicted conformational states on the millisecond timescale.

, isJats=true)