Designed peptides that assemble into cross-α amyloid-like structures

July 30th, 2018 by Shao-Qing Zhang

Designed peptides that assemble into cross-α amyloid-like structures

Designed peptides that assemble into cross-α amyloid-like structures, Published online: 30 July 2018; doi:10.1038/s41589-018-0105-5

Structural analysis reveals how certain designed peptides adopt unusual spiraling cross-α amyloid-like structures and also rearrange to helical polymers upon mutation of small nonpolar residues that are critical for packing and stabilization.
  • Posted in Nat Chem Biol, Publications
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Monitoring hippocampal glycine with the computationally designed optical sensor GlyFS

July 30th, 2018 by William H. Zhang

Monitoring hippocampal glycine with the computationally designed optical sensor GlyFS

Monitoring hippocampal glycine with the computationally designed optical sensor GlyFS, Published online: 30 July 2018; doi:10.1038/s41589-018-0108-2

A genetically encoded FRET-based optical sensor generated from a computational design approach can monitor hippocampal glycine levels in brain tissue to determine differences between spines and shafts and changes induced by high- and low-frequency stimulation.
  • Posted in Nat Chem Biol, Publications
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A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells

July 30th, 2018 by Esther Braselmann

A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells

A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells, Published online: 30 July 2018; doi:10.1038/s41589-018-0103-7

A new riboswitch-based RNA sensor called Riboglow binds to quenched fluorescent probes to induce fluorescence turn-on. Riboglow enables tagging and tracking of mRNA and short noncoding RNAs with different colored fluorophores in live mammalian cells.
  • Posted in Nat Chem Biol, Publications
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Gramibactin is a bacterial siderophore with a diazeniumdiolate ligand system

July 30th, 2018 by Ron Hermenau

Gramibactin is a bacterial siderophore with a diazeniumdiolate ligand system

Gramibactin is a bacterial siderophore with a diazeniumdiolate ligand system, Published online: 30 July 2018; doi:10.1038/s41589-018-0101-9

Plant-associated rhizosphere bacteria produce gramibactin, a cyclic lipodepsipeptide siderophore that tightly binds iron via an unexpected functional group, the N-nitrosohydroxylamine (diazeniumdiolate) moieties of the amino acid graminine.
  • Posted in Nat Chem Biol, Publications
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[ASAP] Robustness of a Reconstituted <italic toggle=”yes”>Escherichia coli</italic> Protein Translation System Analyzed by Computational Modeling

July 25th, 2018 by Tomoaki Matsuura, Kazufumi Hosoda, Yoshihiro Shimizu

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.8b00228
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The molecular language of membraneless organelles [Cell Biology]

July 25th, 2018 by Edward Gomes, James Shorter

Eukaryotic cells organize their intracellular components into organelles that can be membrane-bound or membraneless. A large number of membraneless organelles, including nucleoli, Cajal bodies, P-bodies, and stress granules, exist as liquid droplets within the cell and arise from the condensation of cellular material in a process termed liquid-liquid phase separation (LLPS). Beyond a mere organizational tool, concentrating cellular components into membraneless organelles tunes biochemical reactions and improves cellular fitness during stress. In this review, we provide an overview of the molecular underpinnings of the formation and regulation of these membraneless organelles. This molecular understanding explains emergent properties of these membraneless organelles and shines new light on neurodegenerative diseases, which may originate from disturbances in LLPS and membraneless organelles.

[ASAP] Investigation of Solvent Hydron Exchange in the Reaction Catalyzed by the Antibiotic Resistance Protein Cfr

July 22nd, 2018 by Matthew R. Bauerle, Tyler L. Grove, Squire J. Booker

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Biochemistry
DOI: 10.1021/acs.biochem.8b00347

[ASAP] Tight Translational Control Using Site-Specific Unnatural Amino Acid Incorporation with Positive Feedback Gene Circuits

July 19th, 2018 by Yusuke Kato

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.8b00204
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[ASAP] Kinetics and Degradation Mechanism of Adrenaline Derivative CpQ in Diluted Aqueous Solutions

July 18th, 2018 by Andrei Blasko, James Tam, Sajeevi Gunasekera, Ale Eboreime

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Biochemistry
DOI: 10.1021/acs.biochem.8b00462

[ASAP] Complete Coupled Binding–Folding Pathway of the Intrinsically Disordered Transcription Factor Protein Brinker Revealed by Molecular Dynamics Simulations and Markov State Modeling

July 18th, 2018 by Andrew P. Collins, Peter C. Anderson

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Biochemistry
DOI: 10.1021/acs.biochem.8b00441