Understanding the Molecular Mechanisms of the CRISPR Toolbox Using Single Molecule Approaches

February 9th, 2018 by Digvijay Singh and Taekjip Ha

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ACS Chemical Biology
DOI: 10.1021/acschembio.7b00905

Development of Activity-Based Chemical Probes for Human Sirtuins

February 8th, 2018 by Elysian Graham, Stacia Rymarchyk, Marci Wood and Yana Cen

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ACS Chemical Biology
DOI: 10.1021/acschembio.7b00754

Toward a Microparticle-Based System for Pooled Assays of Small Molecules in Cellular Contexts

February 8th, 2018 by Carrie E. Yozwiak, Tal Hirschhorn and Brent R. Stockwell

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ACS Chemical Biology
DOI: 10.1021/acschembio.8b00043

Ubiquitin-conjugating enzyme E2 D1 (Ube2D1) mediates lysine-independent ubiquitination of the E3 ubiquitin ligase March-I [Protein Synthesis and Degradation]

February 1st, 2018 by Lei Lei, Joanna Bandola-Simon, Paul A. Roche

March-I is a membrane-bound E3 ubiquitin ligase belonging to the membrane-associated RING-CH (March) family. March-I ubiquitinates and down-regulates expression of major histocompatibility complex (MHC) class II and cluster of differentiation 86 (CD86) in antigen presenting cells. March-I expression is regulated both transcriptionally and post-translationally and it has been reported that the March-I is ubiquitinated and that this ubiquitination contributes to March-I turnover. However, the molecular mechanism regulating March-I ubiquitination and the importance of March-I's E3 ligase activity for March-I ubiquitination are not fully understood. Here we confirmed that although March-I is ubiquitinated, it is not ubiquitinated on a lysine residue as a lysine-less March-I variant was ubiquitinated similarly to wild-type March-I. We found that March-I E3 ligase activity is not required for its ubiquitination and does not regulate March-I protein expression, suggesting that March-I does not undergo autoubiquitination. Knocking down ubiquitin-conjugating enzyme E2 D1 (Ube2D1) impaired March-I ubiquitination, increased March-I expression, and enhanced March-I-dependent downregulation of MHC class II proteins. Taken together, our results suggest that March-I undergoes lysine-independent ubiquitination by an as yet unidentified E3 ubiquitin ligase that together with Ube2D1 regulates March-I expression.
  • Posted in Journal of Biological Chemistry, Publications
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Prediction of Hot Spots at Myeloid Cell Leukemia-1–Inhibitor Interface Using Energy Estimation and Alanine Scanning Mutagenesis

February 1st, 2018 by Parthiban Marimuthu and Kalaimathy Singaravelu

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Biochemistry
DOI: 10.1021/acs.biochem.7b01048

Partial DNA-guided Cas9 enables genome editing with reduced off-target activity

January 29th, 2018 by Hao Yin

Partial DNA-guided Cas9 enables genome editing with reduced off-target activity

Partial DNA-guided Cas9 enables genome editing with reduced off-target activity, Published online: 29 January 2018; doi:10.1038/nchembio.2559

Partial substitution of CRISPR RNAs with DNA nucleotides retains CRISPR–Cas9 genome editing activity while enhancing efficiency and specificity within cells, suggesting that DNA–RNA hybrids may be economical reagents for targeted genome editing.
  • Posted in Nat Chem Biol, Publications
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Rewiring T-cell responses to soluble factors with chimeric antigen receptors

January 29th, 2018 by ZeNan L Chang

Rewiring T-cell responses to soluble factors with chimeric antigen receptors

Rewiring T-cell responses to soluble factors with chimeric antigen receptors, Published online: 29 January 2018; doi:10.1038/nchembio.2565

Chimeric antigen receptor (CAR)-expressing T cells were engineered to recognize soluble protein ligands that, by inducing CAR dimerization, mechanically couple ligand binding and receptor signaling to produce immune effector molecules.
  • Posted in Nat Chem Biol, Publications
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Lytic xylan oxidases from wood-decay fungi unlock biomass degradation

January 29th, 2018 by Marie Couturier

Lytic xylan oxidases from wood-decay fungi unlock biomass degradation

Lytic xylan oxidases from wood-decay fungi unlock biomass degradation, Published online: 29 January 2018; doi:10.1038/nchembio.2558

A new type of fungal lytic polysaccharide monooxygenase (LPMO) catalyzes the oxidative degradation of xylan components of cellulosic biomass and offers potential in wood biorefining.
  • Posted in Nat Chem Biol, Publications
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Genetically Encoded Circuit for Remote Regulation of Cell Migration by Magnetic Fields

January 26th, 2018 by Abdullah A. Mosabbir and Kevin Truong

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.7b00415

Stringency of Synthetic Promoter Sequences in Clostridium Revealed and Circumvented by Tuning Promoter Library Mutation Rates

January 26th, 2018 by Paweł M. Mordaka and John T. Heap

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.7b00398
  • Posted in ACS Synthetic Biology, Publications
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