De Novo Biosynthesis of Glutarate via α-Keto Acid Carbon Chain Extension and Decarboxylation Pathway in Escherichia coli

June 23rd, 2017 by Jian Wang, Yifei Wu, Xinxiao Sun, Qipeng Yuan and Yajun Yan

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.7b00136
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Targeting the N terminus for site-selective protein modification

June 20th, 2017 by Christian B Rosen

Nature Chemical Biology 13, 697 (2017). doi:10.1038/nchembio.2416

Authors: Christian B Rosen & Matthew B Francis

Metal homeostasis: Pumping iron

June 20th, 2017 by Grant Miura

Nature Chemical Biology 13, 693 (2017). doi:10.1038/nchembio.2423

Author: Grant Miura

Antimicrobials: Targeting fungal chromatin

June 20th, 2017 by Stéphane Larochelle

Nature Chemical Biology 13, 693 (2017). doi:10.1038/nchembio.2424

Author: Stéphane Larochelle

Circadian regulation: Switching periods

June 20th, 2017 by Grant Miura

Nature Chemical Biology 13, 693 (2017). doi:10.1038/nchembio.2426

Author: Grant Miura

Synthetic biology: Playing favorites

June 20th, 2017 by Ross Cloney

Nature Chemical Biology 13, 693 (2017). doi:10.1038/nchembio.2425

Author: Ross Cloney

Peptidoglycan: Another brick in the wall

June 20th, 2017 by Anthony J Clarke

Nature Chemical Biology 13, 695 (2017). doi:10.1038/nchembio.2419

Author: Anthony J Clarke

Lipid II embodies the bricks used to build the essential bacterial cell wall component peptidoglycan. A facile new procedure for preparation of species-specific Lipid II in high yields can now be used to unlock the door to antibiotic discovery.

Cpf1 proteins excise CRISPR RNAs from mRNA transcripts in mammalian cells

June 19th, 2017 by Guocai Zhong

Nature Chemical Biology 13, 839 (2017). doi:10.1038/nchembio.2410

Authors: Guocai Zhong, Haimin Wang, Yujun Li, Mai H Tran & Michael Farzan

Cpf1 is a CRISPR effector protein that has greater specificity than Streptococcus pyogenes Cas9 (SpCas9) in genome-editing applications. Here we show that Lachnospiraceae bacterium (Lb) and Acidaminococus sp. (As) Cpf1 orthologs have RNase activities that can excise multiple CRISPR RNAs (crRNAs) from a single RNA polymerase II–driven RNA transcript expressed in mammalian cells. This property simplifies modification of multiple genomic targets and can be used to increase the efficiency of Cpf1-mediated editing.

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A heme-dependent enzyme forms the nitrogen–nitrogen bond in piperazate

June 19th, 2017 by Yi-Ling Du

Nature Chemical Biology 13, 836 (2017). doi:10.1038/nchembio.2411

Authors: Yi-Ling Du, Hai-Yan He, Melanie A Higgins & Katherine S Ryan

Molecules containing a nitrogen–nitrogen (N–N) linkage have a variety of structures and biological activities; however, no enzyme has yet been demonstrated to catalyze N–N bond formation in an organic molecule. Here we report that the heme-dependent enzyme KtzT from Kutzneria sp. 744 catalyzes N–N bond formation in the biosynthesis of piperazate, a building block for nonribosomal peptides.

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A scaffold protein that chaperones a cysteine-sulfenic acid in H2O2 signaling

June 19th, 2017 by Antoine Bersweiler

Nature Chemical Biology 13, 909 (2017). doi:10.1038/nchembio.2412

Authors: Antoine Bersweiler, Benoît D'Autréaux, Hortense Mazon, Alexandre Kriznik, Gemma Belli, Agnès Delaunay-Moisan, Michel B Toledano & Sophie Rahuel-Clermont

  • Posted in Nat Chem Biol, Publications
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