Kinetochore regulation: Let there be light

August 14th, 2017 by Ana C Figueiredo

Nature Chemical Biology 13, 1058 (2017). doi:10.1038/nchembio.2464

Authors: Ana C Figueiredo & Helder Maiato

Kinetochores form the critical interface with spindle microtubules that accounts for chromosome movement and segregation fidelity during mitosis. Spatial and temporal control of motor protein and checkpoint signaling at kinetochores is now possible with a new set of optogenetic tools.

Colibactin assembly line enzymes use S-adenosylmethionine to build a cyclopropane ring

August 7th, 2017 by Li Zha

Nature Chemical Biology 13, 1063 (2017). doi:10.1038/nchembio.2448

Authors: Li Zha, Yindi Jiang, Matthew T Henke, Matthew R Wilson, Jennifer X Wang, Neil L Kelleher & Emily P Balskus

Despite containing an α-amino acid, the versatile cofactor S-adenosylmethionine (SAM) is not a known building block for nonribosomal peptide synthetase (NRPS) assembly lines. Here we report an unusual NRPS module from colibactin biosynthesis that uses SAM for amide bond formation and subsequent cyclopropanation. Our findings showcase a new use for SAM and reveal a novel biosynthetic route to a functional group that likely mediates colibactin's genotoxicity.

  • Posted in Nat Chem Biol, Publications
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Bisphosphoglycerate mutase controls serine pathway flux via 3-phosphoglycerate

August 7th, 2017 by Rob C Oslund

Nature Chemical Biology 13, 1081 (2017). doi:10.1038/nchembio.2453

Authors: Rob C Oslund, Xiaoyang Su, Michael Haugbro, Jung-Min Kee, Mark Esposito, Yael David, Boyuan Wang, Eva Ge, David H Perlman, Yibin Kang, Tom W Muir & Joshua D Rabinowitz

  • Posted in Nat Chem Biol, Publications
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A genetically encoded tool for manipulation of NADP+/NADPH in living cells

August 7th, 2017 by Valentin Cracan

Nature Chemical Biology 13, 1088 (2017). doi:10.1038/nchembio.2454

Authors: Valentin Cracan, Denis V Titov, Hongying Shen, Zenon Grabarek & Vamsi K Mootha

  • Posted in Nat Chem Biol, Publications
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Molecular basis for the unusual ring reconstruction in fungal meroterpenoid biogenesis

July 31st, 2017 by Takahiro Mori

Nature Chemical Biology 13, 1066 (2017). doi:10.1038/nchembio.2443

Authors: Takahiro Mori, Taiki Iwabuchi, Shotaro Hoshino, Hang Wang, Yudai Matsuda & Ikuro Abe

  • Posted in Nat Chem Biol, Publications
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Modulating the DNA polymerase β reaction equilibrium to dissect the reverse reaction

July 31st, 2017 by David D Shock

Nature Chemical Biology 13, 1074 (2017). doi:10.1038/nchembio.2450

Authors: David D Shock, Bret D Freudenthal, William A Beard & Samuel H Wilson

  • Posted in Nat Chem Biol, Publications
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Copper import in Escherichia coli by the yersiniabactin metallophore system

July 24th, 2017 by Eun-Ik Koh

Nature Chemical Biology 13, 1016 (2017). doi:10.1038/nchembio.2441

Authors: Eun-Ik Koh, Anne E Robinson, Nilantha Bandara, Buck E Rogers & Jeffrey P Henderson

  • Posted in Nat Chem Biol, Publications
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FRET monitoring of a nonribosomal peptide synthetase

July 24th, 2017 by Jonas Alfermann

Nature Chemical Biology 13, 1009 (2017). doi:10.1038/nchembio.2435

Authors: Jonas Alfermann, Xun Sun, Florian Mayerthaler, Thomas E Morrell, Eva Dehling, Gerrit Volkmann, Tamiki Komatsuzaki, Haw Yang & Henning D Mootz

Toxicity and repair of DNA adducts produced by the natural product yatakemycin

July 24th, 2017 by Elwood A Mullins

Nature Chemical Biology 13, 1002 (2017). doi:10.1038/nchembio.2439

Authors: Elwood A Mullins, Rongxin Shi & Brandt F Eichman

  • Posted in Nat Chem Biol, Publications
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Peptidomimetic inhibitors of APC–Asef interaction block colorectal cancer migration

July 24th, 2017 by Haiming Jiang

Nature Chemical Biology 13, 994 (2017). doi:10.1038/nchembio.2442

Authors: Haiming Jiang, Rong Deng, Xiuyan Yang, Jialin Shang, Shaoyong Lu, Yanlong Zhao, Kun Song, Xinyi Liu, Qiufen Zhang, Yu Chen, Y Eugene Chinn, Geng Wu, Jian Li, Guoqiang Chen, Jianxiu Yu & Jian Zhang

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