tRNA tracking for direct measurements of protein synthesis kinetics in live cells

May 16th, 2018 by Ivan L. Volkov

tRNA tracking for direct measurements of protein synthesis kinetics in live cells

tRNA tracking for direct measurements of protein synthesis kinetics in live cells, Published online: 16 May 2018; doi:10.1038/s41589-018-0063-y

Combination of single-molecule tracking experiments and machine-learning approaches to monitor diffusional state transitions between ribosome-bound and free tRNAs allows codon resolution measurements of translation kinetics.
  • Posted in Nat Chem Biol, Publications
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Next-generation biocontainment systems for engineered organisms

May 16th, 2018 by Jeong Wook Lee

Next-generation biocontainment systems for engineered organisms

Next-generation biocontainment systems for engineered organisms, Published online: 16 May 2018; doi:10.1038/s41589-018-0056-x

Synthetic biology offers innovative approaches for engineering biological systems, but also supports the development of biocontainment strategies that ensure the safe application of genetically modified organisms.

Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases

May 7th, 2018 by Weston Kightlinger

Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases

Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases, Published online: 07 May 2018; doi:10.1038/s41589-018-0051-2

The GlycoSCORES method, which involves cell-free protein expression and substrate-site profiling of glycosyltransferase enzymes by SAMDI–MS, enables the identification of glycosylation tags for glycoengineering efforts.
  • Posted in Nat Chem Biol, Publications
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Publisher Correction: A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cells

May 4th, 2018 by Aaron H. Nile

Publisher Correction: A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cells

Publisher Correction: A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cells, Published online: 04 May 2018; doi:10.1038/s41589-018-0069-5

Publisher Correction: A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cells
  • Posted in Nat Chem Biol, Publications
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Publisher Correction: Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET

May 4th, 2018 by Daria M. Shcherbakova

Publisher Correction: Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET

Publisher Correction: Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET, Published online: 04 May 2018; doi:10.1038/s41589-018-0070-z

Publisher Correction: Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET
  • Posted in Nat Chem Biol, Publications
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Shared strategies for β-lactam catabolism in the soil microbiome

April 30th, 2018 by Terence S. Crofts

Shared strategies for β-lactam catabolism in the soil microbiome

Shared strategies for β-lactam catabolism in the soil microbiome, Published online: 30 April 2018; doi:10.1038/s41589-018-0052-1

A β-lactamase, a novel type of amidase, and the phenylacetic acid catabolon comprise a catabolic pathway, revealed by genomic and transcriptomic analysis, that enables multiple soil bacteria to use β-lactam antibiotics as a carbon source.

Evolution of cyclohexadienyl dehydratase from an ancestral solute-binding protein

April 23rd, 2018 by Ben E. Clifton

Evolution of cyclohexadienyl dehydratase from an ancestral solute-binding protein

Evolution of cyclohexadienyl dehydratase from an ancestral solute-binding protein, Published online: 23 April 2018; doi:10.1038/s41589-018-0043-2

Ancestral protein reconstruction, with structural and biochemical analysis, illustrates the evolution of a solute-binding protein to cyclohexadienyl dehydratase through incorporation of a catalytic residue and gradual reshaping of the binding site.
  • Posted in Nat Chem Biol, Publications
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Generalized extracellular molecule sensor platform for programming cellular behavior

April 23rd, 2018 by Leo Scheller

Generalized extracellular molecule sensor platform for programming cellular behavior

Generalized extracellular molecule sensor platform for programming cellular behavior, Published online: 23 April 2018; doi:10.1038/s41589-018-0046-z

Engineered erythropoietin receptor scaffolds equipped with extracellular sensor domains and modular intracellular domains that couple to endogenous signaling pathways enable modular reprogramming of designer membrane-bound receptors.
  • Posted in Nat Chem Biol, Publications
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Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET

April 23rd, 2018 by Daria M. Shcherbakova

Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET

Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET, Published online: 23 April 2018; doi:10.1038/s41589-018-0044-1

The monomeric near-infrared (NIR) fluorescent protein miRFP720 enables development of fully NIR Förster resonance energy transfer (FRET) biosensors compatible with CFP–YFP FRET biosensors and blue–green optogenetic tools without optical cross-talk.
  • Posted in Nat Chem Biol, Publications
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Evolution of chalcone isomerase from a noncatalytic ancestor

April 23rd, 2018 by Miriam Kaltenbach

Evolution of chalcone isomerase from a noncatalytic ancestor

Evolution of chalcone isomerase from a noncatalytic ancestor, Published online: 23 April 2018; doi:10.1038/s41589-018-0042-3

Ancestral sequence inference, directed evolution, structural analysis, NMR, and molecular dynamics simulations illuminate how enantioselective activity arises during the evolutionary trajectory of chalcone isomerase from a noncatalytic ancestor.