Synergistic, collaterally sensitive β-lactam combinations suppress resistance in MRSA

September 14th, 2015 by Patrick R Gonzales

Nature Chemical Biology 11, 855 (2015). doi:10.1038/nchembio.1911

Authors: Patrick R Gonzales, Mitchell W Pesesky, Renee Bouley, Anna Ballard, Brent A Biddy, Mark A Suckow, William R Wolter, Valerie A Schroeder, Carey-Ann D Burnham, Shahriar Mobashery, Mayland Chang & Gautam Dantas

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Suppressors of superoxide production from mitochondrial complex III

September 14th, 2015 by Adam L Orr

Nature Chemical Biology 11, 834 (2015). doi:10.1038/nchembio.1910

Authors: Adam L Orr, Leonardo Vargas, Carolina N Turk, Janine E Baaten, Jason T Matzen, Victoria J Dardov, Stephen J Attle, Jing Li, Douglas C Quackenbush, Renata L S Goncalves, Irina V Perevoshchikova, H Michael Petrassi, Shelly L Meeusen, Edward K Ainscow & Martin D Brand

Mitochondrial electron transport drives ATP synthesis but also generates reactive oxygen species, which are both cellular signals and damaging oxidants. Superoxide production by respiratory complex III is implicated in diverse signaling events and pathologies, but its role remains controversial. Using high-throughput screening, we identified compounds that selectively eliminate superoxide production by complex III without altering oxidative phosphorylation; they modulate retrograde signaling including cellular responses to hypoxic and oxidative stress.

A biomimetic approach for enhancing the in vivo half-life of peptides

September 7th, 2015 by Sravan C Penchala

Nature Chemical Biology 11, 793 (2015). doi:10.1038/nchembio.1907

Authors: Sravan C Penchala, Mark R Miller, Arindom Pal, Jin Dong, Nikhil R Madadi, Jinghang Xie, Hyun Joo, Jerry Tsai, Patrick Batoon, Vyacheslav Samoshin, Andreas Franz, Trever Cox, Jesse Miles, William K Chan, Miki S Park & Mamoun M Alhamadsheh

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Chemical proteomics reveals a γH2AX-53BP1 interaction in the DNA damage response

September 7th, 2015 by Ralph E Kleiner

Nature Chemical Biology 11, 807 (2015). doi:10.1038/nchembio.1908

Authors: Ralph E Kleiner, Priyanka Verma, Kelly R Molloy, Brian T Chait & Tarun M Kapoor

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Extracellular 4′-phosphopantetheine is a source for intracellular coenzyme A synthesis

August 31st, 2015 by Balaji Srinivasan

Nature Chemical Biology 11, 784 (2015). doi:10.1038/nchembio.1906

Authors: Balaji Srinivasan, Madina Baratashvili, Marianne van der Zwaag, Bart Kanon, Cristina Colombelli, Roald A Lambrechts, Onno Schaap, Ellen A Nollen, Ajda Podgoršek, Gregor Kosec, Hrvoje Petković, Susan Hayflick, Valeria Tiranti, Dirk-Jan Reijngoud, Nicola A Grzeschik & Ody C M Sibon

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Monobody-mediated alteration of enzyme specificity

August 31st, 2015 by Shun-ichi Tanaka

Nature Chemical Biology 11, 762 (2015). doi:10.1038/nchembio.1896

Authors: Shun-ichi Tanaka, Tetsuya Takahashi, Akiko Koide, Satoru Ishihara, Satoshi Koikeda & Shohei Koide

Current methods for engineering enzymes modify enzymes themselves and require a detailed mechanistic understanding or a high-throughput assay. Here, we describe a new approach where catalytic properties are modulated with synthetic binding proteins, termed monobodies, directed to an unmodified enzyme. Using the example of a β-galactosidase from Bacillus circulans, we efficiently identified monobodies that restricted its substrates for its transgalactosylation reaction and selectively enhanced the production of small oligosaccharide prebiotics.

Structural insights into xenobiotic and inhibitor binding to human aldehyde oxidase

August 31st, 2015 by Catarina Coelho

Nature Chemical Biology 11, 779 (2015). doi:10.1038/nchembio.1895

Authors: Catarina Coelho, Alessandro Foti, Tobias Hartmann, Teresa Santos-Silva, Silke Leimkühler & Maria João Romão

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N-terminal domains mediate [2Fe-2S] cluster transfer from glutaredoxin-3 to anamorsin

August 24th, 2015 by Lucia Banci

Nature Chemical Biology 11, 772 (2015). doi:10.1038/nchembio.1892

Authors: Lucia Banci, Simone Ciofi-Baffoni, Karolina Gajda, Riccardo Muzzioli, Riccardo Peruzzini & Julia Winkelmann

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Human calprotectin is an iron-sequestering host-defense protein

August 24th, 2015 by Toshiki G Nakashige

Nature Chemical Biology 11, 765 (2015). doi:10.1038/nchembio.1891

Authors: Toshiki G Nakashige, Bo Zhang, Carsten Krebs & Elizabeth M Nolan

Cancer metabolism: Two sides of a coin

August 18th, 2015 by Grant Miura

Nature Chemical Biology 11, 632 (2015). doi:10.1038/nchembio.1899

Author: Grant Miura