Functional and structural characterization of a heparanase

November 2nd, 2015 by Lisa Bohlmann

Nature Chemical Biology 11, 955 (2015). doi:10.1038/nchembio.1956

Authors: Lisa Bohlmann, Gregory D Tredwell, Xing Yu, Chih-Wei Chang, Thomas Haselhorst, Moritz Winger, Jeffrey C Dyason, Robin J Thomson, Joe Tiralongo, Ifor R Beacham, Helen Blanchard & Mark von Itzstein

We report the structural and functional characterization of a novel heparanase (BpHep) from the invasive pathogenic bacterium Burkholderia pseudomallei (Bp), showing ∼24% sequence identity with human heparanase (hHep). Site-directed mutagenesis studies confirmed the active site resi-dues essential for activity, and we found that BpHep has specificity for heparan sulfate. Finally, we describe the first heparanase X-ray crystal structure, which provides new insight into both substrate recognition and inhibitor design.

A selective chemical probe for exploring the role of CDK8 and CDK19 in human disease

October 26th, 2015 by Trevor Dale

Nature Chemical Biology 11, 973 (2015). doi:10.1038/nchembio.1952

Authors: Trevor Dale, Paul A Clarke, Christina Esdar, Dennis Waalboer, Olajumoke Adeniji-Popoola, Maria-Jesus Ortiz-Ruiz, Aurélie Mallinger, Rahul S Samant, Paul Czodrowski, Djordje Musil, Daniel Schwarz, Klaus Schneider, Mark Stubbs, Ken Ewan, Elizabeth Fraser, Robert TePoele, Will Court, Gary Box, Melanie Valenti, Alexis de Haven Brandon, Sharon Gowan, Felix Rohdich, Florence Raynaud, Richard Schneider, Oliver Poeschke, Andree Blaukat, Paul Workman, Kai Schiemann, Suzanne A Eccles, Dirk Wienke & Julian Blagg

  • Posted in Nat Chem Biol, Publications
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Crystal structure of group II intron domain 1 reveals a template for RNA assembly

October 26th, 2015 by Chen Zhao

Nature Chemical Biology 11, 967 (2015). doi:10.1038/nchembio.1949

Authors: Chen Zhao, Kanagalaghatta R Rajashankar, Marco Marcia & Anna Marie Pyle

  • Posted in Nat Chem Biol, Publications
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Plant toxicology: Defusing the explosive

October 20th, 2015 by Grant Miura

Nature Chemical Biology 11, 829 (2015). doi:10.1038/nchembio.1947

Author: Grant Miura

Voices of chemical biology

October 20th, 2015 by Nature Chemical Biology - Issue - nature.com science feeds

Nature Chemical Biology 11, 826 (2015). doi:10.1038/nchembio.1951

We asked a collection of chemical biologists: “What advice would you give to a junior scientist interested in pursuing a career in chemical biology?”

Metabolomics: Budgeting on a diet

October 20th, 2015 by Grant Miura

Nature Chemical Biology 11, 828 (2015). doi:10.1038/nchembio.1944

Author: Grant Miura

RNA profiling: A better miR trap

October 20th, 2015 by Terry L Sheppard

Nature Chemical Biology 11, 828 (2015). doi:10.1038/nchembio.1942

Author: Terry L Sheppard

Microbiology: Membranes get the gold

October 20th, 2015 by Mirella Bucci

Nature Chemical Biology 11, 829 (2015). doi:10.1038/nchembio.1946

Author: Mirella Bucci

Antibiotics: Synergistic MRSA combinations

October 20th, 2015 by Karen Bush

Nature Chemical Biology 11, 832 (2015). doi:10.1038/nchembio.1935

Author: Karen Bush

Meropenem/piperacillin/tazobactam is a triple β-lactam combination that kills MRSA in vitro and in a mouse model through a novel synergistic mechanism of action. Similar activity for other carbapenem/β-lactam combinations suggests that MRSA infections might be treatable with combinations of established β-lactams currently classified as ineffective against MRSA.

Corrigendum: The promise and peril of chemical probes

October 20th, 2015 by Cheryl H Arrowsmith

Nature Chemical Biology 11, 887 (2015). doi:10.1038/nchembio1115-887c

Author: Cheryl H Arrowsmith, James E Audia, Christopher Austin, Jonathan Baell, Jonathan Bennett, Julian Blagg, Chas Bountra, Paul E Brennan, Peter J Brown, Mark E Bunnage, Carolyn Buser-Doepner, Robert M Campbell, Adrian J Carter, Philip Cohen, Robert A Copeland, Ben Cravatt, Jayme L Dahlin, Dashyant Dhanak, Aled M Edwards, Mathias Frederiksen, Stephen V Frye, Nathanael Gray, Charles E Grimshaw, David Hepworth, Trevor Howe, Kilian V M Huber, Jian Jin, Stefan Knapp, Joanne D Kotz, Ryan G Kruger, Derek Lowe, Mary M Mader, Brian Marsden, Anke Mueller-Fahrnow, Susanne Müller, Ronan C O'Hagan, John P Overington, Dafydd R Owen, Saul H Rosenberg, Ruth Ross, Bryan Roth, Matthieu Schapira, Stuart L Schreiber, Brian Shoichet, Michael Sundström, Giulio Superti-Furga, Jack Taunton, Leticia Toledo-Sherman, Chris Walpole, Michael A Walters, Timothy M Willson, Paul Workman, Robert N Young & William J Zuercher