Copper regulates rest-activity cycles through the locus coeruleus-norepinephrine system

June 4th, 2018 by Tong Xiao

Copper regulates rest-activity cycles through the locus coeruleus-norepinephrine system

Copper regulates rest-activity cycles through the locus coeruleus-norepinephrine system, Published online: 04 June 2018; doi:10.1038/s41589-018-0062-z

Copper contributes to regulating zebrafish rest–activity cycles through the locus coeruleus system by modulating the biosynthesis of norepinephrine; brain copper deficiency leads to lower levels of both synaptic norepinephrine and daytime activity.
  • Posted in Nat Chem Biol, Publications
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A pathogenesis-related 10 protein catalyzes the final step in thebaine biosynthesis

May 28th, 2018 by Xue Chen

A pathogenesis-related 10 protein catalyzes the final step in thebaine biosynthesis

A pathogenesis-related 10 protein catalyzes the final step in thebaine biosynthesis, Published online: 28 May 2018; doi:10.1038/s41589-018-0059-7

Although the conversion of (7S)-salutaridinol 7-O-acetate to thebaine can occur spontaneously, the identification of a thebaine synthase enzyme that catalyzes the reaction indicates how nature avoids the formation of labile hydroxylated byproducts.
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Genome-wide mapping reveals that deoxyuridine is enriched in the human centromeric DNA

May 21st, 2018 by Xiaoting Shu

Genome-wide mapping reveals that deoxyuridine is enriched in the human centromeric DNA

Genome-wide mapping reveals that deoxyuridine is enriched in the human centromeric DNA, Published online: 21 May 2018; doi:10.1038/s41589-018-0065-9

A genome-wide uracil profiling technology (termed “dU-seq”), based on selective labeling and biotin pull-down, reveals that uracil is enriched at the centromere of the human genome.
  • Posted in Nat Chem Biol, Publications
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Computational redesign of enzymes for regio- and enantioselective hydroamination

May 21st, 2018 by Ruifeng Li

Computational redesign of enzymes for regio- and enantioselective hydroamination

Computational redesign of enzymes for regio- and enantioselective hydroamination, Published online: 21 May 2018; doi:10.1038/s41589-018-0053-0

Computational protein design, without subsequent directed evolution, rapidly provides a set of aspartase variants capable of biocatalytic asymmetric addition of ammonia to substituted acrylates, producing various enantiopure β-amino acids.
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Identification of a <i>S. aureus</i> virulence factor by activity-based protein profiling (ABPP)

May 16th, 2018 by Christian S. Lentz

Identification of a S. aureus virulence factor by activity-based protein profiling (ABPP)

Identification of a <i>S. aureus</i> virulence factor by activity-based protein profiling (ABPP), Published online: 16 May 2018; doi:10.1038/s41589-018-0060-1

ABP profiling identifies uncharacterized S. aureus serine hydrolases, including the surface-localized FphB, which processes lipid ester substrates and is required for infection in vivo. An FphB inhibitor reduces in vivo bacterial load.
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Functional phosphorylation

May 16th, 2018 by Mirella Bucci

Functional phosphorylation

Functional phosphorylation, Published online: 16 May 2018; doi:10.1038/s41589-018-0075-7

Functional phosphorylation

Tracking tRNA packages

May 16th, 2018 by Achillefs N. Kapanidis

Tracking tRNA packages

Tracking tRNA packages, Published online: 16 May 2018; doi:10.1038/s41589-018-0066-8

Fast, single-molecule tracking microscopy monitors transitions between mobile and ribosome-bound fluorescent tRNAs to achieve nucleotide-resolution measurements of translation rates in living cells.

Buffering transition

May 16th, 2018 by Yiyun Song

Buffering transition

Buffering transition, Published online: 16 May 2018; doi:10.1038/s41589-018-0076-6

Buffering transition

Electron handoff

May 16th, 2018 by Caitlin Deane

Electron handoff

Electron handoff, Published online: 16 May 2018; doi:10.1038/s41589-018-0073-9

Electron handoff

Membrane association of monotopic phosphoglycosyl transferase underpins function

May 16th, 2018 by Leah C. Ray

Membrane association of monotopic phosphoglycosyl transferase underpins function

Membrane association of monotopic phosphoglycosyl transferase underpins function, Published online: 16 May 2018; doi:10.1038/s41589-018-0054-z

The structure of a monotopic polyprenol phosphate phosphoglycosyl transferase, PglC, reveals how it interacts with the bacterial membrane and coordinates a reaction between membrane-embedded and soluble substrates during glycoconjugate assembly.
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