Employing a biochemical protecting group for a sustainable indigo dyeing strategy

January 8th, 2018 by Tammy M Hsu

Employing a biochemical protecting group for a sustainable indigo dyeing strategy

Employing a biochemical protecting group for a sustainable indigo dyeing strategy, Published online: 08 January 2018; doi:10.1038/nchembio.2552

An environmentally friendly approach to indigo production is facilitated by the characterization of a plant indoxyl glucosyltransferase, which converts the unstable indoxyl precursor into indican by addition of a glucose protecting group.
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Na<sup>+</sup>-mimicking ligands stabilize the inactive state of leukotriene B<sub>4</sub> receptor BLT1

January 8th, 2018 by Tetsuya Hori

Na+-mimicking ligands stabilize the inactive state of leukotriene B4 receptor BLT1

Na<sup>+</sup>-mimicking ligands stabilize the inactive state of leukotriene B<sub>4</sub> receptor BLT1, Published online: 08 January 2018; doi:10.1038/nchembio.2547

A structure of leukotriene B4 receptor BLT1 bound with a benzamidine-containing compound, BIIL260, reveals an inverse-agonist mechanism involving ligand binding in the sodium ion-centered water cluster adjacent to the conserved orthosteric site of class A GPCRs.
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An OPR3-independent pathway uses 4,5-didehydrojasmonate for jasmonate synthesis

January 1st, 2018 by Andrea Chini

An OPR3-independent pathway uses 4,5-didehydrojasmonate for jasmonate synthesis

An OPR3-independent pathway uses 4,5-didehydrojasmonate for jasmonate synthesis, Published online: 01 January 2018; doi:10.1038/nchembio.2540

OPR3 is required to reduce the JA-Ile precursor OPDA. Analyses of JA levels in a loss-of-function opr3-3 mutant identified an OPR3-independent pathway for JA-Ile biosynthesis, based on OPDA conversion to 4,5-ddh-JA and reduction to JA by OPR2.
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Accessing chemical diversity from the uncultivated symbionts of small marine animals

January 1st, 2018 by Thomas E Smith

Accessing chemical diversity from the uncultivated symbionts of small marine animals

Accessing chemical diversity from the uncultivated symbionts of small marine animals, Published online: 01 January 2018; doi:10.1038/nchembio.2537

A combination of spectroscopy, metagenomics, and synthetic biology enables the characterization of the antiviral divamides, a class of lanthipeptide natural products in which even minor changes in structure lead to different biological activities.
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A role for 2-Cys peroxiredoxins in facilitating cytosolic protein thiol oxidation

December 18th, 2017 by Sarah Stöcker

A role for 2-Cys peroxiredoxins in facilitating cytosolic protein thiol oxidation

A role for 2-Cys peroxiredoxins in facilitating cytosolic protein thiol oxidation, Published online: 18 December 2017; doi:10.1038/nchembio.2536

Cytosolic 2-Cys peroxiredoxins can enable, rather than compete with, rapid thiol oxidation by relaying H2O2-derived oxidizing equivalents to other proteins, suggesting a broadened role for peroxiredoxins as sensors and transmitters of H2O2 signals.
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Pharmacological perturbation of CDK9 using selective CDK9 inhibition or degradation

December 18th, 2017 by Calla M Olson

Pharmacological perturbation of CDK9 using selective CDK9 inhibition or degradation

Pharmacological perturbation of CDK9 using selective CDK9 inhibition or degradation, Published online: 18 December 2017; doi:10.1038/nchembio.2538

A selective small-molecule degrader of CDK9 was generated by conjugating an imide to SNS-032, a promiscuous ATP-site-directed CDK binder. The pharmacological consequences of CDK9 degradation versus inhibition were compared.
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Expression system for structural and functional studies of human glycosylation enzymes

December 18th, 2017 by Kelley W Moremen

Expression system for structural and functional studies of human glycosylation enzymes

Expression system for structural and functional studies of human glycosylation enzymes, Published online: 18 December 2017; doi:10.1038/nchembio.2539

A modular protein expression system enables the structural and functional characterization of human glycosyltransferases, glycoside hydrolases and other carbohydrate-modifying enzymes.
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RNA modifications: Ribosomes get decorated

December 12th, 2017 by Mirella Bucci

RNA modifications: Ribosomes get decorated

RNA modifications: Ribosomes get decorated, Published online: 12 December 2017; doi:10.1038/nchembio.2543

RNA modifications: Ribosomes get decorated

Notch signaling: A sweet strategy

December 12th, 2017 by Tetsuya Okajima

Notch signaling: A sweet strategy

Notch signaling: A sweet strategy, Published online: 12 December 2017; doi:10.1038/nchembio.2532

Glycosylation of Notch receptors regulates ligand-induced Notch signaling, which is essential for normal development in animals. Fucose analogs targeting Notch glycosylation serve as ligand-specific Notch inhibitors and facilitate the understanding of how O-glycan regulates Notch–ligand interactions.

Microbiology: Trapping Rac1

December 12th, 2017 by Karin Kuehnel

Microbiology: Trapping Rac1

Microbiology: Trapping Rac1, Published online: 12 December 2017; doi:10.1038/nchembio.2541

Microbiology: Trapping Rac1