Pharmacological targeting of the Wdr5-MLL interaction in C/EBPα N-terminal leukemia

July 13th, 2015 by Florian Grebien

Nature Chemical Biology 11, 571 (2015). doi:10.1038/nchembio.1859

Authors: Florian Grebien, Masoud Vedadi, Matthäus Getlik, Roberto Giambruno, Amit Grover, Roberto Avellino, Anna Skucha, Sarah Vittori, Ekaterina Kuznetsova, David Smil, Dalia Barsyte-Lovejoy, Fengling Li, Gennadiy Poda, Matthieu Schapira, Hong Wu, Aiping Dong, Guillermo Senisterra, Alexey Stukalov, Kilian V M Huber, Andreas Schönegger, Richard Marcellus, Martin Bilban, Christoph Bock, Peter J Brown, Johannes Zuber, Keiryn L Bennett, Rima Al-awar, Ruud Delwel, Claus Nerlov, Cheryl H Arrowsmith & Giulio Superti-Furga

  • Posted in Nat Chem Biol, Publications
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DNA replication: Unlocking the secrets of fork arrest

July 6th, 2015 by Jun Fan

Nature Chemical Biology 11, 550 (2015). doi:10.1038/nchembio.1860

Authors: Jun Fan & Terence R Strick

A powerful, high-throughput single-molecule approach to probe the nanoscale mechanical properties of the Tus–Ter protein–DNA complex reveals that the Tus–Ter-induced lock in unzipping at the nonpermissive face requires only DNA strand separation and involves a progressive strengthening of the Tus–Ter complex.

Strand separation establishes a sustained lock at the Tus–Ter replication fork barrier

July 6th, 2015 by Bojk A Berghuis

Nature Chemical Biology 11, 579 (2015). doi:10.1038/nchembio.1857

Authors: Bojk A Berghuis, David Dulin, Zhi-Qiang Xu, Theo van Laar, Bronwen Cross, Richard Janissen, Slobodan Jergic, Nicholas E Dixon, Martin Depken & Nynke H Dekker

  • Posted in Nat Chem Biol, Publications
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Stereochemical inversion of (S)-reticuline by a cytochrome P450 fusion in opium poppy

July 1st, 2015 by Scott C Farrow

Nature Chemical Biology 11, 728 (2015). doi:10.1038/nchembio.1879

Authors: Scott C Farrow, Jillian M Hagel, Guillaume A W Beaudoin, Darcy C Burns & Peter J Facchini

  • Posted in Nat Chem Biol, Publications
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Structural basis of enzymatic benzene ring reduction

June 29th, 2015 by Tobias Weinert

Nature Chemical Biology 11, 586 (2015). doi:10.1038/nchembio.1849

Authors: Tobias Weinert, Simona G Huwiler, Johannes W Kung, Sina Weidenweber, Petra Hellwig, Hans-Joachim Stärk, Till Biskup, Stefan Weber, Julien J H Cotelesage, Graham N George, Ulrich Ermler & Matthias Boll

5-Formylcytosine can be a stable DNA modification in mammals

June 22nd, 2015 by Martin Bachman

Nature Chemical Biology 11, 555 (2015). doi:10.1038/nchembio.1848

Authors: Martin Bachman, Santiago Uribe-Lewis, Xiaoping Yang, Heather E Burgess, Mario Iurlaro, Wolf Reik, Adele Murrell & Shankar Balasubramanian

5-Formylcytosine (5fC) is a rare base found in mammalian DNA and thought to be involved in active DNA demethylation. Here, we show that developmental dynamics of 5fC levels in mouse DNA differ from those of 5-hydroxymethylcytosine (5hmC), and using stable isotope labeling in vivo, we show that 5fC can be a stable DNA modification. These results suggest that 5fC has functional roles in DNA that go beyond being a demethylation intermediate.

Structural analysis of leader peptide binding enables leader-free cyanobactin processing

June 22nd, 2015 by Jesko Koehnke

Nature Chemical Biology 11, 558 (2015). doi:10.1038/nchembio.1841

Authors: Jesko Koehnke, Greg Mann, Andrew F Bent, Hannes Ludewig, Sally Shirran, Catherine Botting, Tomas Lebl, Wael E Houssen, Marcel Jaspars & James H Naismith

  • Posted in Nat Chem Biol, Publications
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Biogenesis of reactive sulfur species for signaling by hydrogen sulfide oxidation pathways

June 17th, 2015 by Tatiana V Mishanina

Nature Chemical Biology 11, 457 (2015). doi:10.1038/nchembio.1834

Authors: Tatiana V Mishanina, Marouane Libiad & Ruma Banerjee

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Iron-sulfur proteins hiding in plain sight

June 17th, 2015 by Tracey A Rouault

Nature Chemical Biology 11, 442 (2015). doi:10.1038/nchembio.1843

Author: Tracey A Rouault

Recent studies suggest that iron-sulfur (Fe-S) proteins may be unexpectedly abundant and functionally diverse in mammalian cells, but their identification still remains difficult. The use of informatics along with traditional spectroscopic analyses could be key to discovering new Fe-S proteins and validating their functional roles.

Interactomics: Connecting the dots

June 17th, 2015 by Joshua M. Finkelstein

Nature Chemical Biology 11, 449 (2015). doi:10.1038/nchembio.1855

Author: Joshua M. Finkelstein