Chemical proteomics reveals a γH2AX-53BP1 interaction in the DNA damage response

September 7th, 2015 by Ralph E Kleiner

Nature Chemical Biology 11, 807 (2015). doi:10.1038/nchembio.1908

Authors: Ralph E Kleiner, Priyanka Verma, Kelly R Molloy, Brian T Chait & Tarun M Kapoor

  • Posted in Nat Chem Biol, Publications
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Extracellular 4′-phosphopantetheine is a source for intracellular coenzyme A synthesis

August 31st, 2015 by Balaji Srinivasan

Nature Chemical Biology 11, 784 (2015). doi:10.1038/nchembio.1906

Authors: Balaji Srinivasan, Madina Baratashvili, Marianne van der Zwaag, Bart Kanon, Cristina Colombelli, Roald A Lambrechts, Onno Schaap, Ellen A Nollen, Ajda Podgoršek, Gregor Kosec, Hrvoje Petković, Susan Hayflick, Valeria Tiranti, Dirk-Jan Reijngoud, Nicola A Grzeschik & Ody C M Sibon

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Monobody-mediated alteration of enzyme specificity

August 31st, 2015 by Shun-ichi Tanaka

Nature Chemical Biology 11, 762 (2015). doi:10.1038/nchembio.1896

Authors: Shun-ichi Tanaka, Tetsuya Takahashi, Akiko Koide, Satoru Ishihara, Satoshi Koikeda & Shohei Koide

Current methods for engineering enzymes modify enzymes themselves and require a detailed mechanistic understanding or a high-throughput assay. Here, we describe a new approach where catalytic properties are modulated with synthetic binding proteins, termed monobodies, directed to an unmodified enzyme. Using the example of a β-galactosidase from Bacillus circulans, we efficiently identified monobodies that restricted its substrates for its transgalactosylation reaction and selectively enhanced the production of small oligosaccharide prebiotics.

Structural insights into xenobiotic and inhibitor binding to human aldehyde oxidase

August 31st, 2015 by Catarina Coelho

Nature Chemical Biology 11, 779 (2015). doi:10.1038/nchembio.1895

Authors: Catarina Coelho, Alessandro Foti, Tobias Hartmann, Teresa Santos-Silva, Silke Leimkühler & Maria João Romão

  • Posted in Nat Chem Biol, Publications
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N-terminal domains mediate [2Fe-2S] cluster transfer from glutaredoxin-3 to anamorsin

August 24th, 2015 by Lucia Banci

Nature Chemical Biology 11, 772 (2015). doi:10.1038/nchembio.1892

Authors: Lucia Banci, Simone Ciofi-Baffoni, Karolina Gajda, Riccardo Muzzioli, Riccardo Peruzzini & Julia Winkelmann

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Human calprotectin is an iron-sequestering host-defense protein

August 24th, 2015 by Toshiki G Nakashige

Nature Chemical Biology 11, 765 (2015). doi:10.1038/nchembio.1891

Authors: Toshiki G Nakashige, Bo Zhang, Carsten Krebs & Elizabeth M Nolan

Cancer metabolism: Two sides of a coin

August 18th, 2015 by Grant Miura

Nature Chemical Biology 11, 632 (2015). doi:10.1038/nchembio.1899

Author: Grant Miura

Corrigendum: Sterol metabolism controls TH17 differentiation by generating endogenous RORγ agonists

August 18th, 2015 by Xiao Hu

Nature Chemical Biology 11, 741 (2015). doi:10.1038/nchembio0915-741b

Author: Xiao Hu, Yahong Wang, Ling-Yang Hao, Xikui Liu, Chuck A Lesch, Brian M Sanchez, Jay M Wendling, Rodney W Morgan, Tom D Aicher, Laura L Carter, Peter L Toogood & Gary D Glick

  • Posted in Nat Chem Biol, Publications
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Reinvigorating natural product combinatorial biosynthesis with synthetic biology

August 18th, 2015 by Eunji Kim

Nature Chemical Biology 11, 649 (2015). doi:10.1038/nchembio.1893

Authors: Eunji Kim, Bradley S Moore & Yeo Joon Yoon

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Corrigendum: Local and macroscopic electrostatic interactions in single α-helices

August 18th, 2015 by Emily G Baker

Nature Chemical Biology 11, 741 (2015). doi:10.1038/nchembio0915-741e

Author: Emily G Baker, Gail J Bartlett, Matthew P Crump, Richard B Sessions, Noah Linden, Charl F J Faul & Derek N Woolfson

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