Virology: MicroRNA-lipid one-upmanship

November 17th, 2015 by Patricia A Thibault

Nature Chemical Biology 11, 905 (2015). doi:10.1038/nchembio.1953

Authors: Patricia A Thibault & Joyce A Wilson

25-Hydroxycholesterol induces miR-185 to suppress lipid and cholesterol synthesis pathways and inhibit viruses such as hepatitis C virus (HCV), that use host membranes to propagate. HCV counteracts this by suppressing miR-185 expression to drive increased cellular lipid content.

Intrinsically disordered proteins: I like to move it, move it

November 17th, 2015 by Joshua M. Finkelstein

Nature Chemical Biology 11, 902 (2015). doi:10.1038/nchembio.1972

Author: Joshua M. Finkelstein

Branched-chain amino acid catabolism fuels adipocyte differentiation and lipogenesis

November 16th, 2015 by Courtney R Green

Nature Chemical Biology 12, 15 (2016). doi:10.1038/nchembio.1961

Authors: Courtney R Green, Martina Wallace, Ajit S Divakaruni, Susan A Phillips, Anne N Murphy, Theodore P Ciaraldi & Christian M Metallo

  • Posted in Nat Chem Biol, Publications
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Allosteric regulation of G protein–coupled receptor activity by phospholipids

November 16th, 2015 by Rosie Dawaliby

Nature Chemical Biology 12, 35 (2016). doi:10.1038/nchembio.1960

Authors: Rosie Dawaliby, Cataldo Trubbia, Cédric Delporte, Matthieu Masureel, Pierre Van Antwerpen, Brian K Kobilka & Cédric Govaerts

  • Posted in Nat Chem Biol, Publications
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Class D β-lactamases do exist in Gram-positive bacteria

November 9th, 2015 by Marta Toth

Nature Chemical Biology 12, 9 (2016). doi:10.1038/nchembio.1950

Authors: Marta Toth, Nuno Tiago Antunes, Nichole K Stewart, Hilary Frase, Monolekha Bhattacharya, Clyde A Smith & Sergei B Vakulenko

Structural determinants of reductive terpene cyclization in iridoid biosynthesis

November 9th, 2015 by Hajo Kries

Nature Chemical Biology 12, 6 (2016). doi:10.1038/nchembio.1955

Authors: Hajo Kries, Lorenzo Caputi, Clare E M Stevenson, Mohammed O Kamileen, Nathaniel H Sherden, Fernando Geu-Flores, David M Lawson & Sarah E O'Connor

The carbon skeleton of ecologically and pharmacologically important iridoid monoterpenes is formed in a reductive cyclization reaction unrelated to canonical terpene cyclization. Here we report the crystal structure of the recently discovered iridoid cyclase (from Catharanthus roseus) bound to a mechanism-inspired inhibitor that illuminates substrate binding and catalytic function of the enzyme. Key features that distinguish iridoid synthase from its close homolog progesterone 5β-reductase are highlighted.

  • Posted in Nat Chem Biol, Publications
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Functional and structural characterization of a heparanase

November 2nd, 2015 by Lisa Bohlmann

Nature Chemical Biology 11, 955 (2015). doi:10.1038/nchembio.1956

Authors: Lisa Bohlmann, Gregory D Tredwell, Xing Yu, Chih-Wei Chang, Thomas Haselhorst, Moritz Winger, Jeffrey C Dyason, Robin J Thomson, Joe Tiralongo, Ifor R Beacham, Helen Blanchard & Mark von Itzstein

We report the structural and functional characterization of a novel heparanase (BpHep) from the invasive pathogenic bacterium Burkholderia pseudomallei (Bp), showing ∼24% sequence identity with human heparanase (hHep). Site-directed mutagenesis studies confirmed the active site resi-dues essential for activity, and we found that BpHep has specificity for heparan sulfate. Finally, we describe the first heparanase X-ray crystal structure, which provides new insight into both substrate recognition and inhibitor design.

A selective chemical probe for exploring the role of CDK8 and CDK19 in human disease

October 26th, 2015 by Trevor Dale

Nature Chemical Biology 11, 973 (2015). doi:10.1038/nchembio.1952

Authors: Trevor Dale, Paul A Clarke, Christina Esdar, Dennis Waalboer, Olajumoke Adeniji-Popoola, Maria-Jesus Ortiz-Ruiz, Aurélie Mallinger, Rahul S Samant, Paul Czodrowski, Djordje Musil, Daniel Schwarz, Klaus Schneider, Mark Stubbs, Ken Ewan, Elizabeth Fraser, Robert TePoele, Will Court, Gary Box, Melanie Valenti, Alexis de Haven Brandon, Sharon Gowan, Felix Rohdich, Florence Raynaud, Richard Schneider, Oliver Poeschke, Andree Blaukat, Paul Workman, Kai Schiemann, Suzanne A Eccles, Dirk Wienke & Julian Blagg

  • Posted in Nat Chem Biol, Publications
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Crystal structure of group II intron domain 1 reveals a template for RNA assembly

October 26th, 2015 by Chen Zhao

Nature Chemical Biology 11, 967 (2015). doi:10.1038/nchembio.1949

Authors: Chen Zhao, Kanagalaghatta R Rajashankar, Marco Marcia & Anna Marie Pyle

  • Posted in Nat Chem Biol, Publications
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Voices of chemical biology

October 20th, 2015 by Nature Chemical Biology - Issue - nature.com science feeds

Nature Chemical Biology 11, 826 (2015). doi:10.1038/nchembio.1951

We asked a collection of chemical biologists: “What advice would you give to a junior scientist interested in pursuing a career in chemical biology?”