The Taf14 YEATS domain is a reader of histone crotonylation

April 18th, 2016 by Forest H Andrews

Nature Chemical Biology 12, 396 (2016). doi:10.1038/nchembio.2065

Authors: Forest H Andrews, Stephen A Shinsky, Erin K Shanle, Joseph B Bridgers, Anneliese Gest, Ian K Tsun, Krzysztof Krajewski, Xiaobing Shi, Brian D Strahl & Tatiana G Kutateladze

The discovery of new histone modifications is unfolding at startling rates; however, the identification of effectors capable of interpreting these modifications has lagged behind. Here we report the YEATS domain as an effective reader of histone lysine crotonylation, an epigenetic signature associated with active transcription. We show that the Taf14 YEATS domain engages crotonyllysine via a unique π–π–π-stacking mechanism and that other YEATS domains have crotonyllysine-binding activity.

Real-time monitoring of basal H2O2 levels with peroxiredoxin-based probes

April 18th, 2016 by Bruce Morgan

Nature Chemical Biology 12, 437 (2016). doi:10.1038/nchembio.2067

Authors: Bruce Morgan, Koen Van Laer, Theresa N E Owusu, Daria Ezeriņa, Daniel Pastor-Flores, Prince Saforo Amponsah, Anja Tursch & Tobias P Dick

  • Posted in Nat Chem Biol, Publications
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A synthetic biochemistry module for production of bio-based chemicals from glucose

April 11th, 2016 by Paul H Opgenorth

Nature Chemical Biology 12, 393 (2016). doi:10.1038/nchembio.2062

Authors: Paul H Opgenorth, Tyler P Korman & James U Bowie

Synthetic biochemistry, the cell-free production of biologically based chemicals, is a potentially high-yield, flexible alternative to in vivo metabolic engineering. To limit costs, cell-free systems must be designed to operate continuously with minimal addition of feedstock chemicals. We describe a robust, efficient synthetic glucose breakdown pathway and implement it for the production of bioplastic. The system's performance suggests that synthetic biochemistry has the potential to become a viable industrial alternative.

  • Posted in Nat Chem Biol, Publications
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Optimized second-generation CRY2–CIB dimerizers and photoactivatable Cre recombinase

April 11th, 2016 by Amir Taslimi

Nature Chemical Biology 12, 425 (2016). doi:10.1038/nchembio.2063

Authors: Amir Taslimi, Brian Zoltowski, Jose G Miranda, Gopal P Pathak, Robert M Hughes & Chandra L Tucker

  • Posted in Nat Chem Biol, Publications
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Optogenetic oligomerization of Rab GTPases regulates intracellular membrane trafficking

April 11th, 2016 by Mai Khanh Nguyen

Nature Chemical Biology 12, 431 (2016). doi:10.1038/nchembio.2064

Authors: Mai Khanh Nguyen, Cha Yeon Kim, Jin Man Kim, Byung Ouk Park, Sangkyu Lee, Hyerim Park & Won Do Heo

  • Posted in Nat Chem Biol, Publications
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Plant-like biosynthesis of isoquinoline alkaloids in Aspergillus fumigatus

April 11th, 2016 by Joshua A Baccile

Nature Chemical Biology 12, 419 (2016). doi:10.1038/nchembio.2061

Authors: Joshua A Baccile, Joseph E Spraker, Henry H Le, Eileen Brandenburger, Christian Gomez, Jin Woo Bok, Juliane Macheleidt, Axel A Brakhage, Dirk Hoffmeister, Nancy P Keller & Frank C Schroeder

  • Posted in Nat Chem Biol, Publications
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Decoding Polo-like kinase 1 signaling along the kinetochore–centromere axis

April 4th, 2016 by Robert F Lera

Nature Chemical Biology 12, 411 (2016). doi:10.1038/nchembio.2060

Authors: Robert F Lera, Gregory K Potts, Aussie Suzuki, James M Johnson, Edward D Salmon, Joshua J Coon & Mark E Burkard

  • Posted in Nat Chem Biol, Publications
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Raft-based interactions of gangliosides with a GPI-anchored receptor

April 4th, 2016 by Naoko Komura

Nature Chemical Biology 12, 402 (2016). doi:10.1038/nchembio.2059

Authors: Naoko Komura, Kenichi G N Suzuki, Hiromune Ando, Miku Konishi, Machi Koikeda, Akihiro Imamura, Rahul Chadda, Takahiro K Fujiwara, Hisae Tsuboi, Ren Sheng, Wonhwa Cho, Koichi Furukawa, Keiko Furukawa, Yoshio Yamauchi, Hideharu Ishida, Akihiro Kusumi & Makoto Kiso

Essential biphasic role for JAK3 catalytic activity in IL-2 receptor signaling

March 28th, 2016 by Geoffrey A Smith

Nature Chemical Biology 12, 373 (2016). doi:10.1038/nchembio.2056

Authors: Geoffrey A Smith, Kenji Uchida, Arthur Weiss & Jack Taunton

  • Posted in Nat Chem Biol, Publications
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AIG1 and ADTRP are atypical integral membrane hydrolases that degrade bioactive FAHFAs

March 28th, 2016 by William H Parsons

Nature Chemical Biology 12, 367 (2016). doi:10.1038/nchembio.2051

Authors: William H Parsons, Matthew J Kolar, Siddhesh S Kamat, Armand B Cognetta III, Jonathan J Hulce, Enrique Saez, Barbara B Kahn, Alan Saghatelian & Benjamin F Cravatt

  • Posted in Nat Chem Biol, Publications
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