Synthesis of 11-carbon terpenoids in yeast using protein and metabolic engineering

November 14th, 2018 by Codruta Ignea

Synthesis of 11-carbon terpenoids in yeast using protein and metabolic engineering

Synthesis of 11-carbon terpenoids in yeast using protein and metabolic engineering, Published online: 14 November 2018; doi:10.1038/s41589-018-0166-5

Yeast engineered to produce the six-carbon terpene building block 2-methyl-geranyl diphosphate, coupled with terpene synthases engineered to accept this noncanonical substrate, enables the biosynthesis of a collection of new C11 terpenoid scaffolds.
  • Posted in Nat Chem Biol, Publications
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Failure to convert

November 14th, 2018 by Grant Miura

Failure to convert

Failure to convert, Published online: 14 November 2018; doi:10.1038/s41589-018-0172-7

Failure to convert

Identification of <i>Chaoborus</i> kairomone chemicals that induce defences in <i>Daphnia</i>

November 14th, 2018 by Linda C. Weiss

Identification of Chaoborus kairomone chemicals that induce defences in Daphnia

Identification of <i>Chaoborus</i> kairomone chemicals that induce defences in <i>Daphnia</i>, Published online: 14 November 2018; doi:10.1038/s41589-018-0164-7

The freshwater microcrustacean Daphnia pulex forms defensive neckteeth in response to a collection of chemical cues (the kairomone), now identified as certain fatty acids conjugated to glutamine, released during digestion by its predator Chaoborus.
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HIP1R targets PD-L1 to lysosomal degradation to alter T cell–mediated cytotoxicity

November 5th, 2018 by Huanbin Wang

HIP1R targets PD-L1 to lysosomal degradation to alter T cell–mediated cytotoxicity

HIP1R targets PD-L1 to lysosomal degradation to alter T cell–mediated cytotoxicity, Published online: 05 November 2018; doi:10.1038/s41589-018-0161-x

HIP1R directly interacts with PD-L1 and targets PD-L1 for lysosomal degradation. Development of a rationally designed peptide incorporating the PD-L1 binding sequence of HIP1R with a lysosomal targeting sequence promotes PD-L1 degradation.
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Identification of a cellularly active SIRT6 allosteric activator

October 29th, 2018 by Zhimin Huang

Identification of a cellularly active SIRT6 allosteric activator

Identification of a cellularly active SIRT6 allosteric activator, Published online: 29 October 2018; doi:10.1038/s41589-018-0150-0

The use of an allosteric drug-design method resulted in the identification of a first-in-class cellularly active SIRT6 activator that induces cell-cycle arrest in the G0–G1 phase, thus suppressing proliferation in human hepatocellular carcinoma cells.

The multicatalytic compartment of propionyl-CoA synthase sequesters a toxic metabolite

October 29th, 2018 by Iria Bernhardsgrütter

The multicatalytic compartment of propionyl-CoA synthase sequesters a toxic metabolite

The multicatalytic compartment of propionyl-CoA synthase sequesters a toxic metabolite, Published online: 29 October 2018; doi:10.1038/s41589-018-0153-x

Structural and biochemical analysis of propionyl-CoA synthase reveals that it forms a reaction chamber containing three active sites, which sequesters the reactive intermediate acrylyl-CoA during the conversion of 3-hydroxypropionate to propionyl-CoA.
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Structure-guided development of YEATS domain inhibitors by targeting π-π-π stacking

October 29th, 2018 by Xin Li

Structure-guided development of YEATS domain inhibitors by targeting π-π-π stacking

Structure-guided development of YEATS domain inhibitors by targeting π-π-π stacking, Published online: 29 October 2018; doi:10.1038/s41589-018-0144-y

An inhibitor of the YEATS domain was developed by targeting a unique π-π-π stacking in the YEATS–Kcr recognition. An ENL YEATS-selective inhibitor, XL-13m, helps probe the YEATS-dependent role of ENL in the leukemogenic transcription program.
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Enzyme promiscuity drives branched-chain fatty acid synthesis in adipose tissues

October 16th, 2018 by Martina Wallace

Enzyme promiscuity drives branched-chain fatty acid synthesis in adipose tissues

Enzyme promiscuity drives branched-chain fatty acid synthesis in adipose tissues, Published online: 16 October 2018; doi:10.1038/s41589-018-0132-2

mmBCFAs are endogenous fatty acids synthesized from BCAAs by brown and white adipose tissue via CrAT and FASN promiscuity. BCAA catabolism and mmBCFA lipogenesis are decreased by obesity-induced adipose hypoxia and influenced by the microbiome.
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No more ESKAPE

October 16th, 2018 by Mirella Bucci

No more ESKAPE

No more ESKAPE, Published online: 16 October 2018; doi:10.1038/s41589-018-0156-7

No more ESKAPE

Small-molecule-based regulation of RNA-delivered circuits in mammalian cells

October 16th, 2018 by Tyler E. Wagner

Small-molecule-based regulation of RNA-delivered circuits in mammalian cells

Small-molecule-based regulation of RNA-delivered circuits in mammalian cells, Published online: 16 October 2018; doi:10.1038/s41589-018-0146-9

Engineering of small-molecule-responsive RNA-binding proteins enables chemical regulation of modified mRNA or RNA replicon expression within mammalian cells for applications in synthetic circuit design and RNA-centered therapeutics.
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