Facile target validation in an animal model with intracellularly expressed monobodies

July 16th, 2018 by Ankit Gupta

Facile target validation in an animal model with intracellularly expressed monobodies

Facile target validation in an animal model with intracellularly expressed monobodies, Published online: 16 July 2018; doi:10.1038/s41589-018-0099-z

The development of a selective and potent monobody to WDR5, a component of the mixed linear leukemia methyltransferase complex, as genetically encoded inhibitor enables suppression of leukemogenesis and confers survival in a mouse leukemia model.
  • Posted in Nat Chem Biol, Publications
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Acetylation blocks DNA damage–induced chromatin ADP-ribosylation

July 16th, 2018 by Glen Liszczak

Acetylation blocks DNA damage–induced chromatin ADP-ribosylation

Acetylation blocks DNA damage–induced chromatin ADP-ribosylation, Published online: 16 July 2018; doi:10.1038/s41589-018-0097-1

Histone H3 serine 10 is found to be the major chromatin acceptor residue for DNA damage–induced ADP-ribosylation and is blocked by specific acetylation sites on PARP1 and/or H3.

PAINTing translation

July 9th, 2018 by Yuichiro Mishima

PAINTing translation

PAINTing translation, Published online: 09 July 2018; doi:10.1038/s41589-018-0102-8

Establishment of the germ cell lineage requires post-transcriptional regulation of mRNAs, yet the underlying molecular mechanisms are not fully understood in vertebrates. A small-molecule inhibitor of germ cell formation reveals a noncanonical translation system used in zebrafish embryos.

Noncanonical translation via deadenylated 3′ UTRs maintains primordial germ cells

July 9th, 2018 by Youngnam N. Jin

Noncanonical translation via deadenylated 3′ UTRs maintains primordial germ cells

Noncanonical translation via deadenylated 3′ UTRs maintains primordial germ cells, Published online: 09 July 2018; doi:10.1038/s41589-018-0098-0

Primordazine inhibits poly(A)-tail-independent noncanonical translation (PAINT) in early zebrafish embryos and in mammalian cells under select conditions, an effect mediated by deadenylated 3ʹ UTRs that results in ablation of primordial germ cells.
  • Posted in Nat Chem Biol, Publications
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Peptide exchange on MHC-I by TAPBPR is driven by a negative allostery release cycle

July 9th, 2018 by Andrew C. McShan

Peptide exchange on MHC-I by TAPBPR is driven by a negative allostery release cycle

Peptide exchange on MHC-I by TAPBPR is driven by a negative allostery release cycle, Published online: 09 July 2018; doi:10.1038/s41589-018-0096-2

Use of NMR to monitor MHC-I dynamics upon binding to the MHC-I chaperone TAPBPR explains the selection of optimal peptide sequences and release of the chaperone from the ternary peptide-MHC-I–TAPBPR complex.
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Publisher Correction: The Jumonji-C oxygenase JMJD7 catalyzes (3<i>S</i>)-lysyl hydroxylation of TRAFAC GTPases

June 27th, 2018 by Suzana Markolovic

Publisher Correction: The Jumonji-C oxygenase JMJD7 catalyzes (3S)-lysyl hydroxylation of TRAFAC GTPases

Publisher Correction: The Jumonji-C oxygenase JMJD7 catalyzes (3<i>S</i>)-lysyl hydroxylation of TRAFAC GTPases, Published online: 27 June 2018; doi:10.1038/s41589-018-0104-6

Publisher Correction: The Jumonji-C oxygenase JMJD7 catalyzes (3S)-lysyl hydroxylation of TRAFAC GTPases
  • Posted in Nat Chem Biol, Publications
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Designing microbial consortia with defined social interactions

June 25th, 2018 by Wentao Kong

Designing microbial consortia with defined social interactions

Designing microbial consortia with defined social interactions, Published online: 25 June 2018; doi:10.1038/s41589-018-0091-7

Synthetic microbial consortia were engineered as experimental models of bacterial interactions within ecosystems, and mathematical models of their behavior were used to design more complex microbial systems with additional interactions.

Sarpagan bridge enzyme has substrate-controlled cyclization and aromatization modes

June 25th, 2018 by Thu-Thuy T. Dang

Sarpagan bridge enzyme has substrate-controlled cyclization and aromatization modes

Sarpagan bridge enzyme has substrate-controlled cyclization and aromatization modes, Published online: 25 June 2018; doi:10.1038/s41589-018-0078-4

Three homologous cytochrome P450s from monoterpene indole alkaloid-producing plants enable the identification of sarpagan bridge enzyme, which catalyzes either cyclization or aromatization to yield sarpagan or β-carboline alkaloids, respectively.
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Metabolic engineering of a carbapenem antibiotic synthesis pathway in <i>Escherichia coli</i>

June 25th, 2018 by Helena Shomar

Metabolic engineering of a carbapenem antibiotic synthesis pathway in Escherichia coli

Metabolic engineering of a carbapenem antibiotic synthesis pathway in <i>Escherichia coli</i>, Published online: 25 June 2018; doi:10.1038/s41589-018-0084-6

Efficient production of a simple carbapenem antibiotic in Escherichia coli is achieved by a combination of feedback-resistant enzymes for increased precursor biosynthesis and inhibition of fatty acid synthesis for tolerance toward the toxic product.
  • Posted in Nat Chem Biol, Publications
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Cleavage of a carbon–fluorine bond by an engineered cysteine dioxygenase

June 25th, 2018 by Jiasong Li

Cleavage of a carbon–fluorine bond by an engineered cysteine dioxygenase

Cleavage of a carbon–fluorine bond by an engineered cysteine dioxygenase, Published online: 25 June 2018; doi:10.1038/s41589-018-0085-5

Engineered variants of cysteine dioxygenase containing a halogen-substituted tyrosine analog provide insights into the process of Cys–Tyr cross-link formation and indicate that the enzyme can catalyze oxidative cleavage of a carbon–fluorine bond.
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