Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase

February 15th, 2019 by Maximilian Fottner

Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase

Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase, Published online: 15 February 2019; doi:10.1038/s41589-019-0227-4

A method combining genetic-code expansion, bioorthogonal Staudinger reduction and sortase-mediated transpeptidation enables site-specific and orthogonal modification of proteins with ubiquitin and SUMO in vitro and in living cells.
  • Posted in Nat Chem Biol, Publications
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A super-powered S-layer

February 15th, 2019 by Caitlin Deane

A super-powered S-layer

A super-powered S-layer, Published online: 15 February 2019; doi:10.1038/s41589-019-0236-3

A super-powered S-layer

Decoding without the cipher

February 15th, 2019 by Amit Kumar Singh Gautam

Decoding without the cipher

Decoding without the cipher, Published online: 15 February 2019; doi:10.1038/s41589-019-0230-9

A new method introduces ubiquitin or ubiquitin-like proteins at specific sites in any protein without the requirement of the cellular ubiquitylation machinery. This will help decipher the code by which these modifications control cellular processes.

Author Correction: Complete reconstitution of the diverse pathways of gentamicin B biosynthesis

February 6th, 2019 by Yeon Hee Ban

Author Correction: Complete reconstitution of the diverse pathways of gentamicin B biosynthesis

Author Correction: Complete reconstitution of the diverse pathways of gentamicin B biosynthesis, Published online: 06 February 2019; doi:10.1038/s41589-019-0232-7

Author Correction: Complete reconstitution of the diverse pathways of gentamicin B biosynthesis
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A class of highly selective inhibitors bind to an active state of PI3Kγ

February 4th, 2019 by Gangadhara Gangadhara

A class of highly selective inhibitors bind to an active state of PI3Kγ

A class of highly selective inhibitors bind to an active state of PI3Kγ, Published online: 04 February 2019; doi:10.1038/s41589-018-0215-0

Combining X-ray structures, surface plasmon resonance and hydrogen-deuterium exchange–mass spectrometry, a class of highly selective inhibitors was found to bind to an active state of PI3Kγ breaking a conformational ‘lock’ important for activation of PI3Kγ.
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Mechanism of regulation and neutralization of the AtaR–AtaT toxin–antitoxin system

February 4th, 2019 by Dukas Jurėnas

Mechanism of regulation and neutralization of the AtaR–AtaT toxin–antitoxin system

Mechanism of regulation and neutralization of the AtaR–AtaT toxin–antitoxin system, Published online: 04 February 2019; doi:10.1038/s41589-018-0216-z

Structural and biochemical analysis of the AtaR–AtaT toxin–antioxin system reveals that AtaR traps AtaT in a precatalytic monomeric state and forms a heterohexameric complex to neutralize AtaT and repress transcription of the operon.
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Small-molecule allosteric inhibitors of BAX

February 4th, 2019 by Thomas P. Garner

Small-molecule allosteric inhibitors of BAX

Small-molecule allosteric inhibitors of BAX, Published online: 04 February 2019; doi:10.1038/s41589-018-0223-0

Using biochemical and NMR studies, a class of small-molecule inhibitors termed BAX activation inhibitors were found to bind directly to a previously unrecognized pocket of inactive BAX and allosterically inhibit conformational changes in BAX.

A chemoproteomic portrait of the oncometabolite fumarate

February 4th, 2019 by Rhushikesh A. Kulkarni

A chemoproteomic portrait of the oncometabolite fumarate

A chemoproteomic portrait of the oncometabolite fumarate, Published online: 04 February 2019; doi:10.1038/s41589-018-0217-y

Chemoproteomic mapping of fumarate sensitive cysteines in a hereditary leiomyomatosis and renal cell carcinoma cell line revealed a critical cysteine in the protein–protein interaction interface of the SWI–SNF complex.

An allosteric MALT1 inhibitor is a molecular corrector rescuing function in an immunodeficient patient

January 28th, 2019 by Jean Quancard

An allosteric MALT1 inhibitor is a molecular corrector rescuing function in an immunodeficient patient

An allosteric MALT1 inhibitor is a molecular corrector rescuing function in an immunodeficient patient, Published online: 28 January 2019; doi:10.1038/s41589-018-0222-1

An allosteric inhibitor of the proteolytic activity of the CBM signalosome complex scaffolding protein MALT1 acts as a pharmacological chaperone to stabilize a mutant MALT1 carried by an immunodeficient patient.
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Bacterial sensors define intracellular free energies for correct enzyme metalation

January 28th, 2019 by Deenah Osman

Bacterial sensors define intracellular free energies for correct enzyme metalation

Bacterial sensors define intracellular free energies for correct enzyme metalation, Published online: 28 January 2019; doi:10.1038/s41589-018-0211-4

The sensitivities of a set of bacterial metal-sensing transcriptional regulators explain how a protein, such as the cobalt chelatase CbiK, is metalated with its cognate metal in cells rather than mis-metalated with more tightly binding metals.
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