A chemical-inducible CRISPR–Cas9 system for rapid control of genome editing

September 12th, 2016 by Kaiwen Ivy Liu

Nature Chemical Biology 12, 980 (2016). doi:10.1038/nchembio.2179

Authors: Kaiwen Ivy Liu, Muhammad Nadzim Bin Ramli, Cheok Wei Ariel Woo, Yuanming Wang, Tianyun Zhao, Xiujun Zhang, Guo Rong Daniel Yim, Bao Yi Chong, Ali Gowher, Mervyn Zi Hao Chua, Jonathan Jung, Jia Hui Jane Lee & Meng How Tan

  • Posted in Nat Chem Biol, Publications
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Lactate metabolism is associated with mammalian mitochondria

September 12th, 2016 by Ying-Jr Chen

Nature Chemical Biology 12, 937 (2016). doi:10.1038/nchembio.2172

Authors: Ying-Jr Chen, Nathaniel G Mahieu, Xiaojing Huang, Manmilan Singh, Peter A Crawford, Stephen L Johnson, Richard W Gross, Jacob Schaefer & Gary J Patti

YidC assists the stepwise and stochastic folding of membrane proteins

September 5th, 2016 by Tetiana Serdiuk

Nature Chemical Biology 12, 911 (2016). doi:10.1038/nchembio.2169

Authors: Tetiana Serdiuk, Dhandayuthapani Balasubramaniam, Junichi Sugihara, Stefania A Mari, H Ronald Kaback & Daniel J Müller

  • Posted in Nat Chem Biol, Publications
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Simultaneous analysis of enzyme structure and activity by kinetic capillary electrophoresis–MS

September 5th, 2016 by Gleb G Mironov

Nature Chemical Biology 12, 918 (2016). doi:10.1038/nchembio.2170

Authors: Gleb G Mironov, Christopher M Clouthier, Abdullah Akbar, Jeffrey W Keillor & Maxim V Berezovski

  • Posted in Nat Chem Biol, Publications
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Inhibition of Mcl-1 through covalent modification of a noncatalytic lysine side chain

September 5th, 2016 by Gizem Akçay

Nature Chemical Biology 12, 931 (2016). doi:10.1038/nchembio.2174

Authors: Gizem Akçay, Matthew A Belmonte, Brian Aquila, Claudio Chuaqui, Alexander W Hird, Michelle L Lamb, Philip B Rawlins, Nancy Su, Sharon Tentarelli, Neil P Grimster & Qibin Su

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Small-molecule WNK inhibition regulates cardiovascular and renal function

September 5th, 2016 by Ken Yamada

Nature Chemical Biology 12, 896 (2016). doi:10.1038/nchembio.2168

Authors: Ken Yamada, Hyi-Man Park, Dean F Rigel, Keith DiPetrillo, Erin J Whalen, Anthony Anisowicz, Michael Beil, James Berstler, Cara Emily Brocklehurst, Debra A Burdick, Shari L Caplan, Michael P Capparelli, Guanjing Chen, Wei Chen, Bethany Dale, Lin Deng, Fumin Fu, Norio Hamamatsu, Kouki Harasaki, Tracey Herr, Peter Hoffmann, Qi-Ying Hu, Waan-Jeng Huang, Neeraja Idamakanti, Hidetomo Imase, Yuki Iwaki, Monish Jain, Jey Jeyaseelan, Mitsunori Kato, Virendar K Kaushik, Darcy Kohls, Vidya Kunjathoor, Daniel LaSala, Jongchan Lee, Jing Liu, Yang Luo, Fupeng Ma, Ruowei Mo, Sarah Mowbray, Muneto Mogi, Flavio Ossola, Pramod Pandey, Sejal J Patel, Swetha Raghavan, Bahaa Salem, Yuka H Shanado, Gary M Trakshel, Gordon Turner, Hiromichi Wakai, Chunhua Wang, Stephen Weldon, Jennifer B Wielicki, Xiaoling Xie, Lingfei Xu, Yukiko I Yagi, Kayo Yasoshima, Jianning Yin, David Yowe, Ji-Hu Zhang, Gang Zheng & Lauren Monovich

The With-No-Lysine (K) (WNK) kinases play a critical role in blood pressure regulation and body fluid and electrolyte homeostasis. Herein, we introduce the first orally bioavailable pan-WNK-kinase inhibitor, WNK463, that exploits unique structural features of the WNK kinases for both affinity and kinase selectivity. In rodent models of hypertension, WNK463 affects blood pressure and body fluid and electro-lyte homeostasis, consistent with WNK-kinase-associated physiology and pathophysiology.

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Overcoming resistance to HER2 inhibitors through state-specific kinase binding

September 5th, 2016 by Chris J Novotny

Nature Chemical Biology 12, 923 (2016). doi:10.1038/nchembio.2171

Authors: Chris J Novotny, Sirkku Pollari, Jin H Park, Mark A Lemmon, Weijun Shen & Kevan M Shokat

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Genetic code expansion in the mouse brain

August 29th, 2016 by Russell J Ernst

Nature Chemical Biology 12, 776 (2016). doi:10.1038/nchembio.2160

Authors: Russell J Ernst, Toke P Krogager, Elizabeth S Maywood, Roberto Zanchi, Václav Beránek, Thomas S Elliott, Nicholas P Barry, Michael H Hastings & Jason W Chin

Site-specific incorporation of non-natural amino acids into proteins, via genetic code expansion with pyrrolysyl tRNA synthetase (PylRS) and tRNAPylCUA pairs (and their evolved derivatives) from Methanosarcina sp., forms the basis of powerful approaches to probe and control protein function in cells and invertebrate organisms. Here we demonstrate that adeno-associated viral delivery of these pairs enables efficient genetic code expansion in primary neuronal culture, organotypic brain slices and the brains of live mice.

Dual action antifungal small molecule modulates multidrug efflux and TOR signaling

August 29th, 2016 by Tanvi Shekhar-Guturja

Nature Chemical Biology 12, 867 (2016). doi:10.1038/nchembio.2165

Authors: Tanvi Shekhar-Guturja, G M Kamal B Gunaherath, E M Kithsiri Wijeratne, Jean-Philippe Lambert, Anna F Averette, Soo Chan Lee, Taeyup Kim, Yong-Sun Bahn, Farida Tripodi, Ron Ammar, Katja Döhl, Karolina Niewola-Staszkowska, Lutz Schmitt, Robbie J Loewith, Frederick P Roth, Dominique Sanglard, David Andes, Corey Nislow, Paola Coccetti, Anne-Claude Gingras, Joseph Heitman, A A Leslie Gunatilaka & Leah E Cowen

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Covalent targeting of remote cysteine residues to develop CDK12 and CDK13 inhibitors

August 29th, 2016 by Tinghu Zhang

Nature Chemical Biology 12, 876 (2016). doi:10.1038/nchembio.2166

Authors: Tinghu Zhang, Nicholas Kwiatkowski, Calla M Olson, Sarah E Dixon-Clarke, Brian J Abraham, Ann K Greifenberg, Scott B Ficarro, Jonathan M Elkins, Yanke Liang, Nancy M Hannett, Theresa Manz, Mingfeng Hao, Bartlomiej Bartkowiak, Arno L Greenleaf, Jarrod A Marto, Matthias Geyer, Alex N Bullock, Richard A Young & Nathanael S Gray

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