Functional mining of transporters using synthetic selections

October 3rd, 2016 by Hans J Genee

Nature Chemical Biology 12, 1015 (2016). doi:10.1038/nchembio.2189

Authors: Hans J Genee, Anne P Bali, Søren D Petersen, Solvej Siedler, Mads T Bonde, Luisa S Gronenberg, Mette Kristensen, Scott J Harrison & Morten O A Sommer

Structural basis for precursor protein–directed ribosomal peptide macrocyclization

September 26th, 2016 by Kunhua Li

Nature Chemical Biology 12, 973 (2016). doi:10.1038/nchembio.2200

Authors: Kunhua Li, Heather L Condurso, Gengnan Li, Yousong Ding & Steven D Bruner

  • Posted in Nat Chem Biol, Publications
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Roger Y. Tsien 1952–2016

September 26th, 2016 by Amy E Palmer

Nature Chemical Biology 12, 887 (2016). doi:10.1038/nchembio.2213

Authors: Amy E Palmer & Jin Zhang

Roger Tsien left us on August 24. His untimely passing has saddened and shocked the scientific community. Roger literally and figuratively brightened our world, illuminated the dark matter of biology, and forever changed our view of the interface of chemistry and biology.

Amino-group carrier-protein-mediated secondary metabolite biosynthesis in Streptomyces

September 26th, 2016 by Fumihito Hasebe

Nature Chemical Biology 12, 967 (2016). doi:10.1038/nchembio.2181

Authors: Fumihito Hasebe, Kenichi Matsuda, Taro Shiraishi, Yushi Futamura, Takeshi Nakano, Takeo Tomita, Ken Ishigami, Hikari Taka, Reiko Mineki, Tsutomu Fujimura, Hiroyuki Osada, Tomohisa Kuzuyama & Makoto Nishiyama

  • Posted in Nat Chem Biol, Publications
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Thermal profiling reveals phenylalanine hydroxylase as an off-target of panobinostat

September 26th, 2016 by Isabelle Becher

Nature Chemical Biology 12, 908 (2016). doi:10.1038/nchembio.2185

Authors: Isabelle Becher, Thilo Werner, Carola Doce, Esther A Zaal, Ina Tögel, Crystal A Khan, Anne Rueger, Marcel Muelbaier, Elsa Salzer, Celia R Berkers, Paul F Fitzpatrick, Marcus Bantscheff & Mikhail M Savitski

We describe a two-dimensional thermal proteome profiling strategy that can be combined with an orthogonal chemoproteomics approach to enable comprehensive target profiling of the marketed histone deacetylase inhibitor panobinostat. The N-hydroxycinnamide moiety is identified as critical for potent and tetrahydrobiopterin-competitive inhibition of phenylalanine hydroxylase leading to increases in phenylalanine and decreases in tyrosine levels. These findings provide a rationale for adverse clinical observations and suggest repurposing of the drug for treatment of tyrosinemia.

  • Posted in Nat Chem Biol, Publications
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Stapled peptides: How to be quick on the uptake

September 20th, 2016 by Joshua A Kritzer

Nature Chemical Biology 12, 764 (2016). doi:10.1038/nchembio.2183

Author: Joshua A Kritzer

Stapled helices are promising compounds for inhibiting intracellular protein–protein interactions, but the discovery of peptides with the key property of cellular uptake has taken place largely through trial and error. A new study defines physicochemical parameters for designing hydrocarbon-stapled helices with a greater likelihood of cellular uptake.

Phospholipids: How to flip a flippase

September 20th, 2016 by Caitlin Deane

Nature Chemical Biology 12, 763 (2016). doi:10.1038/nchembio.2199

Author: Caitlin Deane

Single-molecule biophysics: TALEs spin along, but not around

September 20th, 2016 by Sebastian Becker

Nature Chemical Biology 12, 766 (2016). doi:10.1038/nchembio.2182

Authors: Sebastian Becker & Jens Boch

TALE proteins search for target sequences along a DNA substrate in a uniquely one-dimensional and non-rotating fashion.

Host–microbe interactions: DAO does it

September 20th, 2016 by Mirella Bucci

Nature Chemical Biology 12, 763 (2016). doi:10.1038/nchembio.2196

Author: Mirella Bucci

Corrigendum: Structural biology: HDAC6 finally crystal clear

September 20th, 2016 by Yanli Liu

Nature Chemical Biology 12, 885 (2016). doi:10.1038/nchembio1016-885

Author: Yanli Liu, Li Li & Jinrong Min