Electron handoff

May 16th, 2018 by Caitlin Deane

Electron handoff

Electron handoff, Published online: 16 May 2018; doi:10.1038/s41589-018-0073-9

Electron handoff

Membrane association of monotopic phosphoglycosyl transferase underpins function

May 16th, 2018 by Leah C. Ray

Membrane association of monotopic phosphoglycosyl transferase underpins function

Membrane association of monotopic phosphoglycosyl transferase underpins function, Published online: 16 May 2018; doi:10.1038/s41589-018-0054-z

The structure of a monotopic polyprenol phosphate phosphoglycosyl transferase, PglC, reveals how it interacts with the bacterial membrane and coordinates a reaction between membrane-embedded and soluble substrates during glycoconjugate assembly.
  • Posted in Nat Chem Biol, Publications
  • Comments Off on Membrane association of monotopic phosphoglycosyl transferase underpins function

tRNA tracking for direct measurements of protein synthesis kinetics in live cells

May 16th, 2018 by Ivan L. Volkov

tRNA tracking for direct measurements of protein synthesis kinetics in live cells

tRNA tracking for direct measurements of protein synthesis kinetics in live cells, Published online: 16 May 2018; doi:10.1038/s41589-018-0063-y

Combination of single-molecule tracking experiments and machine-learning approaches to monitor diffusional state transitions between ribosome-bound and free tRNAs allows codon resolution measurements of translation kinetics.
  • Posted in Nat Chem Biol, Publications
  • Comments Off on tRNA tracking for direct measurements of protein synthesis kinetics in live cells

Next-generation biocontainment systems for engineered organisms

May 16th, 2018 by Jeong Wook Lee

Next-generation biocontainment systems for engineered organisms

Next-generation biocontainment systems for engineered organisms, Published online: 16 May 2018; doi:10.1038/s41589-018-0056-x

Synthetic biology offers innovative approaches for engineering biological systems, but also supports the development of biocontainment strategies that ensure the safe application of genetically modified organisms.

Identification of a <i>S. aureus</i> virulence factor by activity-based protein profiling (ABPP)

May 16th, 2018 by Christian S. Lentz

Identification of a S. aureus virulence factor by activity-based protein profiling (ABPP)

Identification of a <i>S. aureus</i> virulence factor by activity-based protein profiling (ABPP), Published online: 16 May 2018; doi:10.1038/s41589-018-0060-1

ABP profiling identifies uncharacterized S. aureus serine hydrolases, including the surface-localized FphB, which processes lipid ester substrates and is required for infection in vivo. An FphB inhibitor reduces in vivo bacterial load.
  • Posted in Nat Chem Biol, Publications
  • Comments Off on Identification of a <i>S. aureus</i> virulence factor by activity-based protein profiling (ABPP)

Functional phosphorylation

May 16th, 2018 by Mirella Bucci

Functional phosphorylation

Functional phosphorylation, Published online: 16 May 2018; doi:10.1038/s41589-018-0075-7

Functional phosphorylation

Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases

May 7th, 2018 by Weston Kightlinger

Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases

Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases, Published online: 07 May 2018; doi:10.1038/s41589-018-0051-2

The GlycoSCORES method, which involves cell-free protein expression and substrate-site profiling of glycosyltransferase enzymes by SAMDI–MS, enables the identification of glycosylation tags for glycoengineering efforts.
  • Posted in Nat Chem Biol, Publications
  • Comments Off on Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases

Publisher Correction: A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cells

May 4th, 2018 by Aaron H. Nile

Publisher Correction: A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cells

Publisher Correction: A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cells, Published online: 04 May 2018; doi:10.1038/s41589-018-0069-5

Publisher Correction: A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cells
  • Posted in Nat Chem Biol, Publications
  • Comments Off on Publisher Correction: A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cells

Publisher Correction: Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET

May 4th, 2018 by Daria M. Shcherbakova

Publisher Correction: Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET

Publisher Correction: Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET, Published online: 04 May 2018; doi:10.1038/s41589-018-0070-z

Publisher Correction: Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET
  • Posted in Nat Chem Biol, Publications
  • Comments Off on Publisher Correction: Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET

Shared strategies for β-lactam catabolism in the soil microbiome

April 30th, 2018 by Terence S. Crofts

Shared strategies for β-lactam catabolism in the soil microbiome

Shared strategies for β-lactam catabolism in the soil microbiome, Published online: 30 April 2018; doi:10.1038/s41589-018-0052-1

A β-lactamase, a novel type of amidase, and the phenylacetic acid catabolon comprise a catabolic pathway, revealed by genomic and transcriptomic analysis, that enables multiple soil bacteria to use β-lactam antibiotics as a carbon source.