Genetic code expansion in the mouse brain

August 29th, 2016 by Russell J Ernst

Nature Chemical Biology 12, 776 (2016). doi:10.1038/nchembio.2160

Authors: Russell J Ernst, Toke P Krogager, Elizabeth S Maywood, Roberto Zanchi, Václav Beránek, Thomas S Elliott, Nicholas P Barry, Michael H Hastings & Jason W Chin

Site-specific incorporation of non-natural amino acids into proteins, via genetic code expansion with pyrrolysyl tRNA synthetase (PylRS) and tRNAPylCUA pairs (and their evolved derivatives) from Methanosarcina sp., forms the basis of powerful approaches to probe and control protein function in cells and invertebrate organisms. Here we demonstrate that adeno-associated viral delivery of these pairs enables efficient genetic code expansion in primary neuronal culture, organotypic brain slices and the brains of live mice.

Fluorogenic probes reveal a role of GLUT4 N-glycosylation in intracellular trafficking

August 22nd, 2016 by Shinya Hirayama

Nature Chemical Biology 12, 853 (2016). doi:10.1038/nchembio.2156

Authors: Shinya Hirayama, Yuichiro Hori, Zsolt Benedek, Tadashi Suzuki & Kazuya Kikuchi

  • Posted in Nat Chem Biol, Publications
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Divergent biosynthesis yields a cytotoxic aminomalonate-containing precolibactin

August 22nd, 2016 by Zhong-Rui Li

Nature Chemical Biology 12, 773 (2016). doi:10.1038/nchembio.2157

Authors: Zhong-Rui Li, Jie Li, Jin-Ping Gu, Jennifer Y H Lai, Brendan M Duggan, Wei-Peng Zhang, Zhi-Long Li, Yong-Xin Li, Rong-Biao Tong, Ying Xu, Dong-Hai Lin, Bradley S Moore & Pei-Yuan Qian

Colibactin is an as-yet-uncharacterized genotoxic secondary metabolite produced by human gut bacteria. Here we report the biosynthetic discovery of two new precolibactin molecules from Escherichia coli, including precolibactin-886, which uniquely incorporates the highly sought genotoxicity-associated aminomalonate building block into its unprecedented macrocyclic structure. This work provides new insights into the biosynthetic logic and mode of action of this colorectal-cancer-linked microbial chemical.

  • Posted in Nat Chem Biol, Publications
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Discovery of LRE1 as a specific and allosteric inhibitor of soluble adenylyl cyclase

August 22nd, 2016 by Lavoisier Ramos-Espiritu

Nature Chemical Biology 12, 838 (2016). doi:10.1038/nchembio.2151

Authors: Lavoisier Ramos-Espiritu, Silke Kleinboelting, Felipe A Navarrete, Antonio Alvau, Pablo E Visconti, Federica Valsecchi, Anatoly Starkov, Giovanni Manfredi, Hannes Buck, Carolina Adura, Jonathan H Zippin, Joop van den Heuvel, J Fraser Glickman, Clemens Steegborn, Lonny R Levin & Jochen Buck

  • Posted in Nat Chem Biol, Publications
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Biophysical determinants for cellular uptake of hydrocarbon-stapled peptide helices

August 22nd, 2016 by Gregory H Bird

Nature Chemical Biology 12, 845 (2016). doi:10.1038/nchembio.2153

Authors: Gregory H Bird, Emanuele Mazzola, Kwadwo Opoku-Nsiah, Margaret A Lammert, Marina Godes, Donna S Neuberg & Loren D Walensky

  • Posted in Nat Chem Biol, Publications
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Oxidation increases the strength of the methionine-aromatic interaction

August 22nd, 2016 by Andrew K Lewis

Nature Chemical Biology 12, 860 (2016). doi:10.1038/nchembio.2159

Authors: Andrew K Lewis, Katie M Dunleavy, Tiffany L Senkow, Cheng Her, Benjamin T Horn, Mark A Jersett, Ryan Mahling, Megan R McCarthy, Gabriella T Perell, Christopher C Valley, Christine B Karim, Jiali Gao, William C K Pomerantz, David D Thomas, Alessandro Cembran, Anne Hinderliter & Jonathan N Sachs

  • Posted in Nat Chem Biol, Publications
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Insights into newly discovered marks and readers of epigenetic information

August 18th, 2016 by Forest H Andrews

Nature Chemical Biology 12, 662 (2016). doi:10.1038/nchembio.2149

Authors: Forest H Andrews, Brian D Strahl & Tatiana G Kutateladze

  • Posted in Nat Chem Biol, Publications
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Innate immunity: A sweet switch

August 18th, 2016 by Terry L. Sheppard

Nature Chemical Biology 12, 657 (2016). doi:10.1038/nchembio.2162

Author: Terry L. Sheppard

Target validation: Switching domains

August 18th, 2016 by Alessio Ciulli

Nature Chemical Biology 12, 659 (2016). doi:10.1038/nchembio.2154

Author: Alessio Ciulli

Chemical probes and drugs often bind to functional domains on disease-relevant proteins. A study suggests a chemical genetic approach to establish on-target effects by swapping the targeted domain, affording resistance to pharmacological inhibition while retaining functionality.

Chemical synthesis: Linking up lysines

August 18th, 2016 by Caitlin Deane

Nature Chemical Biology 12, 657 (2016). doi:10.1038/nchembio.2163

Author: Caitlin Deane