Activity-based probes for functional interrogation of retaining β-glucuronidases

June 5th, 2017 by Liang Wu

Nature Chemical Biology 13, 867 (2017). doi:10.1038/nchembio.2395

Authors: Liang Wu, Jianbing Jiang, Yi Jin, Wouter W Kallemeijn, Chi-Lin Kuo, Marta Artola, Wei Dai, Cas van Elk, Marco van Eijk, Gijsbert A van der Marel, Jeroen D C Codée, Bogdan I Florea, Johannes M F G Aerts, Herman S Overkleeft & Gideon J Davies

  • Posted in Nat Chem Biol, Publications
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Parallel evolution of non-homologous isofunctional enzymes in methionine biosynthesis

June 5th, 2017 by Karine Bastard

Nature Chemical Biology 13, 858 (2017). doi:10.1038/nchembio.2397

Authors: Karine Bastard, Alain Perret, Aline Mariage, Thomas Bessonnet, Agnès Pinet-Turpault, Jean-Louis Petit, Ekaterina Darii, Pascal Bazire, Carine Vergne-Vaxelaire, Clémence Brewee, Adrien Debard, Virginie Pellouin, Marielle Besnard-Gonnet, François Artiguenave, Claudine Médigue, David Vallenet, Antoine Danchin, Anne Zaparucha, Jean Weissenbach, Marcel Salanoubat & Véronique de Berardinis

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Autocatalytic backbone N-methylation in a family of ribosomal peptide natural products

June 5th, 2017 by Niels S van der Velden

Nature Chemical Biology 13, 833 (2017). doi:10.1038/nchembio.2393

Authors: Niels S van der Velden, Noemi Kälin, Maximilian J Helf, Jörn Piel, Michael F Freeman & Markus Künzler

Peptide backbone N-methylation, as seen in cyclosporin A, has been considered to be exclusive to nonribosomal peptides. We have identified the first post-translationally modified peptide or protein harboring internal α-N-methylations through discovery of the genetic locus for the omphalotins, cyclic N-methylated peptides produced by the fungus Omphalotus olearius. We show that iterative autocatalytic activity of an N-methyltransferase fused to its peptide substrate is the signature of a new family of ribosomally encoded metabolites.

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Targeting S-adenosylmethionine biosynthesis with a novel allosteric inhibitor of Mat2A

May 29th, 2017 by Casey L Quinlan

Nature Chemical Biology 13, 785 (2017). doi:10.1038/nchembio.2384

Authors: Casey L Quinlan, Stephen E Kaiser, Ben Bolaños, Dawn Nowlin, Rita Grantner, Shannon Karlicek-Bryant, Jun Li Feng, Stephen Jenkinson, Kevin Freeman-Cook, Stephen G Dann, Xiaoli Wang, Peter A Wells, Valeria R Fantin, Al E Stewart & Stephan K Grant

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Structural basis for high-affinity fluorophore binding and activation by RNA Mango

May 29th, 2017 by Robert J Trachman

Nature Chemical Biology 13, 807 (2017). doi:10.1038/nchembio.2392

Authors: Robert J Trachman, Natalia A Demeshkina, Matthew W L Lau, Shanker Shyam S Panchapakesan, Sunny C Y Jeng, Peter J Unrau & Adrian R Ferré-D'Amaré

  • Posted in Nat Chem Biol, Publications
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Lipid II overproduction allows direct assay of transpeptidase inhibition by β-lactams

May 29th, 2017 by Yuan Qiao

Nature Chemical Biology 13, 793 (2017). doi:10.1038/nchembio.2388

Authors: Yuan Qiao, Veerasak Srisuknimit, Frederick Rubino, Kaitlin Schaefer, Natividad Ruiz, Suzanne Walker & Daniel Kahne

  • Posted in Nat Chem Biol, Publications
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Structural and functional characterization of the hydrogenase-maturation HydF protein

May 29th, 2017 by Giorgio Caserta

Nature Chemical Biology 13, 779 (2017). doi:10.1038/nchembio.2385

Authors: Giorgio Caserta, Ludovic Pecqueur, Agnieszka Adamska-Venkatesh, Cecilia Papini, Souvik Roy, Vincent Artero, Mohamed Atta, Edward Reijerse, Wolfgang Lubitz & Marc Fontecave

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Functional selectivity of GPCR-directed drug action through location bias

May 29th, 2017 by Roshanak Irannejad

Nature Chemical Biology 13, 799 (2017). doi:10.1038/nchembio.2389

Authors: Roshanak Irannejad, Veronica Pessino, Delphine Mika, Bo Huang, Philip B Wedegaertner, Marco Conti & Mark von Zastrow

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Engineering RGB color vision into Escherichia coli

May 22nd, 2017 by Jesus Fernandez-Rodriguez

Nature Chemical Biology 13, 706 (2017). doi:10.1038/nchembio.2390

Authors: Jesus Fernandez-Rodriguez, Felix Moser, Miryoung Song & Christopher A Voigt

Optogenetic tools use colored light to rapidly control gene expression in space and time. We designed a genetically encoded system that gives Escherichia coli the ability to distinguish between red, green, and blue (RGB) light and respond by changing gene expression. We use this system to produce 'color photographs' on bacterial culture plates by controlling pigment production and to redirect metabolic flux by expressing CRISPRi guide RNAs.

Engineering protein stability with atomic precision in a monomeric miniprotein

May 22nd, 2017 by Emily G Baker

Nature Chemical Biology 13, 764 (2017). doi:10.1038/nchembio.2380

Authors: Emily G Baker, Christopher Williams, Kieran L Hudson, Gail J Bartlett, Jack W Heal, Kathryn L Porter Goff, Richard B Sessions, Matthew P Crump & Derek N Woolfson

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