Recurrent RNA motifs as scaffolds for genetically encodable small-molecule biosensors

January 16th, 2017 by Ely B Porter

Nature Chemical Biology 13, 295 (2017). doi:10.1038/nchembio.2278

Authors: Ely B Porter, Jacob T Polaski, Makenna M Morck & Robert T Batey

  • Posted in Nat Chem Biol, Publications
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Chemical proteomics reveals ADP-ribosylation of small GTPases during oxidative stress

January 16th, 2017 by Nathan P Westcott

Nature Chemical Biology 13, 302 (2017). doi:10.1038/nchembio.2280

Authors: Nathan P Westcott, Joseph P Fernandez, Henrik Molina & Howard C Hang

  • Posted in Nat Chem Biol, Publications
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Lysine relay mechanism coordinates intermediate transfer in vitamin B6 biosynthesis

January 16th, 2017 by Matthew J Rodrigues

Nature Chemical Biology 13, 290 (2017). doi:10.1038/nchembio.2273

Authors: Matthew J Rodrigues, Volker Windeisen, Yang Zhang, Gabriela Guédez, Stefan Weber, Marco Strohmeier, Jeremiah W Hanes, Antoine Royant, Gwyndaf Evans, Irmgard Sinning, Steven E Ealick, Tadhg P Begley & Ivo Tews

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Structural and functional insights into asymmetric enzymatic dehydration of alkenols

January 9th, 2017 by Bettina M Nestl

Nature Chemical Biology 13, 275 (2017). doi:10.1038/nchembio.2271

Authors: Bettina M Nestl, Christopher Geinitz, Stephanie Popa, Sari Rizek, Robert J Haselbeck, Rosary Stephen, Michael A Noble, Max-Philipp Fischer, Erik C Ralph, Hoi Ting Hau, Henry Man, Muhiadin Omar, Johan P Turkenburg, Stephen van Dien, Stephanie J Culler, Gideon Grogan & Bernhard Hauer

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Biomimetic spinning of artificial spider silk from a chimeric minispidroin

January 9th, 2017 by Marlene Andersson

Nature Chemical Biology 13, 262 (2017). doi:10.1038/nchembio.2269

Authors: Marlene Andersson, Qiupin Jia, Ana Abella, Xiau-Yeen Lee, Michael Landreh, Pasi Purhonen, Hans Hebert, Maria Tenje, Carol V Robinson, Qing Meng, Gustavo R Plaza, Jan Johansson & Anna Rising

Herein we present a chimeric recombinant spider silk protein (spidroin) whose aqueous solubility equals that of native spider silk dope and a spinning device that is based solely on aqueous buffers, shear forces and lowered pH. The process recapitulates the complex molecular mechanisms that dictate native spider silk spinning and is highly efficient; spidroin from one liter of bacterial shake-flask culture is enough to spin a kilometer of the hitherto toughest as-spun artificial spider silk fiber.

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Visualizing the secondary and tertiary architectural domains of lncRNA RepA

January 9th, 2017 by Fei Liu

Nature Chemical Biology 13, 282 (2017). doi:10.1038/nchembio.2272

Authors: Fei Liu, Srinivas Somarowthu & Anna Marie Pyle

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MraY–antibiotic complex reveals details of tunicamycin mode of action

January 9th, 2017 by Jonna K Hakulinen

Nature Chemical Biology 13, 265 (2017). doi:10.1038/nchembio.2270

Authors: Jonna K Hakulinen, Jenny Hering, Gisela Brändén, Hongming Chen, Arjan Snijder, Margareta Ek & Patrik Johansson

The rapid increase of antibiotic resistance has created an urgent need to develop novel antimicrobial agents. Here we describe the crystal structure of the promising bacterial target phospho-N-acetylmuramoyl–pentapeptide translocase (MraY) in complex with the nucleoside antibiotic tunicamycin. The structure not only reveals the mode of action of several related natural-product antibiotics but also gives an indication on the binding mode of the MraY UDP–MurNAc–pentapeptide and undecaprenyl-phosphate substrates.

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β2-adrenergic receptor control of endosomal PTH receptor signaling via Gβγ

December 26th, 2016 by Frédéric G Jean-Alphonse

Nature Chemical Biology 13, 259 (2017). doi:10.1038/nchembio.2267

Authors: Frédéric G Jean-Alphonse, Vanessa L Wehbi, Jingming Chen, Masaki Noda, Juan M Taboas, Kunhong Xiao & Jean-Pierre Vilardaga

Cells express several G-protein-coupled receptors (GPCRs) at their surfaces, transmitting simultaneous extracellular hormonal and chemical signals into cells. A comprehensive understanding of mechanisms underlying the integrated signaling response induced by distinct GPCRs is thus required. Here we found that the β2-adrenergic receptor, which induces a short cAMP response, prolongs nuclear cAMP and protein kinase A (PKA) activation by promoting endosomal cAMP production in parathyroid hormone (PTH) receptor signaling through the stimulatory action of G protein Gβγ subunits on adenylate cyclase type 2.

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Orthogonal lipid sensors identify transbilayer asymmetry of plasma membrane cholesterol

December 26th, 2016 by Shu-Lin Liu

Nature Chemical Biology 13, 268 (2017). doi:10.1038/nchembio.2268

Authors: Shu-Lin Liu, Ren Sheng, Jae Hun Jung, Li Wang, Ewa Stec, Matthew J O'Connor, Seohyoen Song, Rama Kamesh Bikkavilli, Robert A Winn, Daesung Lee, Kwanghee Baek, Kazumitsu Ueda, Irena Levitan, Kwang-Pyo Kim & Wonhwa Cho

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Orphan receptor ligand discovery by pickpocketing pharmacological neighbors

December 19th, 2016 by Tony Ngo

Nature Chemical Biology 13, 235 (2017). doi:10.1038/nchembio.2266

Authors: Tony Ngo, Andrey V Ilatovskiy, Alastair G Stewart, James L J Coleman, Fiona M McRobb, R Peter Riek, Robert M Graham, Ruben Abagyan, Irina Kufareva & Nicola J Smith

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