Plasticity in binding confers selectivity in ligand-induced protein degradation

June 11th, 2018 by Radosław P. Nowak

Plasticity in binding confers selectivity in ligand-induced protein degradation

Plasticity in binding confers selectivity in ligand-induced protein degradation, Published online: 11 June 2018; doi:10.1038/s41589-018-0055-y

Selectivity of ligand-induced protein degradation and dimerization is conferred by plastic interprotein contacts. Computational protein–protein docking reveals the underlying interprotein contacts to inform the design of a BRD4 selective degrader.
  • Posted in Nat Chem Biol, Publications
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Functional assignment of multiple catabolic pathways for <span class=”small-caps”>d</span>-apiose

June 4th, 2018 by Michael S. Carter

Functional assignment of multiple catabolic pathways for d-apiose

Functional assignment of multiple catabolic pathways for <span class="small-caps">d</span>-apiose, Published online: 04 June 2018; doi:10.1038/s41589-018-0067-7

A bioinformatic strategy beginning with solute-binding proteins involved in sugar transport led to the functional annotation of four previously unknown catabolic pathways of the branched pentose d-apiose.
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Potent and specific Atg8-targeting autophagy inhibitory peptides from giant ankyrins

June 4th, 2018 by Jianchao Li

Potent and specific Atg8-targeting autophagy inhibitory peptides from giant ankyrins

Potent and specific Atg8-targeting autophagy inhibitory peptides from giant ankyrins, Published online: 04 June 2018; doi:10.1038/s41589-018-0082-8

Potent pan-Atg8 or GABARAP-selective inhibitory peptides derived from giant neuronal ankyrin-B and -G effectively block autophagy in cell cultures and in C. elegans spatiotemporally.
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Natural separation of the acyl-CoA ligase reaction results in a non-adenylating enzyme

June 4th, 2018 by Nan Wang

Natural separation of the acyl-CoA ligase reaction results in a non-adenylating enzyme

Natural separation of the acyl-CoA ligase reaction results in a non-adenylating enzyme, Published online: 04 June 2018; doi:10.1038/s41589-018-0061-0

Functional and structural characterization of PtmA2 reveals that it is an unusual non-adenylating acyl-CoA ligase and part of a system wherein the canonical acyl-CoA ligase reaction is separated into two half-reactions performed by distinct enzymes.
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Copper regulates rest-activity cycles through the locus coeruleus-norepinephrine system

June 4th, 2018 by Tong Xiao

Copper regulates rest-activity cycles through the locus coeruleus-norepinephrine system

Copper regulates rest-activity cycles through the locus coeruleus-norepinephrine system, Published online: 04 June 2018; doi:10.1038/s41589-018-0062-z

Copper contributes to regulating zebrafish rest–activity cycles through the locus coeruleus system by modulating the biosynthesis of norepinephrine; brain copper deficiency leads to lower levels of both synaptic norepinephrine and daytime activity.
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A pathogenesis-related 10 protein catalyzes the final step in thebaine biosynthesis

May 28th, 2018 by Xue Chen

A pathogenesis-related 10 protein catalyzes the final step in thebaine biosynthesis

A pathogenesis-related 10 protein catalyzes the final step in thebaine biosynthesis, Published online: 28 May 2018; doi:10.1038/s41589-018-0059-7

Although the conversion of (7S)-salutaridinol 7-O-acetate to thebaine can occur spontaneously, the identification of a thebaine synthase enzyme that catalyzes the reaction indicates how nature avoids the formation of labile hydroxylated byproducts.
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Genome-wide mapping reveals that deoxyuridine is enriched in the human centromeric DNA

May 21st, 2018 by Xiaoting Shu

Genome-wide mapping reveals that deoxyuridine is enriched in the human centromeric DNA

Genome-wide mapping reveals that deoxyuridine is enriched in the human centromeric DNA, Published online: 21 May 2018; doi:10.1038/s41589-018-0065-9

A genome-wide uracil profiling technology (termed “dU-seq”), based on selective labeling and biotin pull-down, reveals that uracil is enriched at the centromere of the human genome.
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Computational redesign of enzymes for regio- and enantioselective hydroamination

May 21st, 2018 by Ruifeng Li

Computational redesign of enzymes for regio- and enantioselective hydroamination

Computational redesign of enzymes for regio- and enantioselective hydroamination, Published online: 21 May 2018; doi:10.1038/s41589-018-0053-0

Computational protein design, without subsequent directed evolution, rapidly provides a set of aspartase variants capable of biocatalytic asymmetric addition of ammonia to substituted acrylates, producing various enantiopure β-amino acids.
  • Posted in Nat Chem Biol, Publications
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Tracking tRNA packages

May 16th, 2018 by Achillefs N. Kapanidis

Tracking tRNA packages

Tracking tRNA packages, Published online: 16 May 2018; doi:10.1038/s41589-018-0066-8

Fast, single-molecule tracking microscopy monitors transitions between mobile and ribosome-bound fluorescent tRNAs to achieve nucleotide-resolution measurements of translation rates in living cells.

Buffering transition

May 16th, 2018 by Yiyun Song

Buffering transition

Buffering transition, Published online: 16 May 2018; doi:10.1038/s41589-018-0076-6

Buffering transition