Site-specific incorporation of phosphotyrosine using an expanded genetic code
June 12th, 2017 by Christian Hoppmann
Nature Chemical Biology 13, 842 (2017). doi:10.1038/nchembio.2406
Authors: Christian Hoppmann, Allison Wong, Bing Yang, Shuwei Li, Tony Hunter, Kevan M Shokat & Lei Wang
Access to phosphoproteins with stoichiometric and site-specific phosphorylation status is key to understanding the role of protein phosphorylation. Here we report an efficient method to generate pure, active phosphotyrosine-containing proteins by genetically encoding a stable phosphotyrosine analog that is convertible to native phosphotyrosine. We demonstrate its general compatibility with proteins of various sizes, phosphotyrosine sites and functions, and reveal a possible role of tyrosine phosphorylation in negative regulation of ubiquitination.
Activity-based probes for functional interrogation of retaining β-glucuronidases
June 5th, 2017 by Liang Wu
Nature Chemical Biology 13, 867 (2017). doi:10.1038/nchembio.2395
Authors: Liang Wu, Jianbing Jiang, Yi Jin, Wouter W Kallemeijn, Chi-Lin Kuo, Marta Artola, Wei Dai, Cas van Elk, Marco van Eijk, Gijsbert A van der Marel, Jeroen D C Codée, Bogdan I Florea, Johannes M F G Aerts, Herman S Overkleeft & Gideon J Davies
Parallel evolution of non-homologous isofunctional enzymes in methionine biosynthesis
June 5th, 2017 by Karine Bastard
Nature Chemical Biology 13, 858 (2017). doi:10.1038/nchembio.2397
Authors: Karine Bastard, Alain Perret, Aline Mariage, Thomas Bessonnet, Agnès Pinet-Turpault, Jean-Louis Petit, Ekaterina Darii, Pascal Bazire, Carine Vergne-Vaxelaire, Clémence Brewee, Adrien Debard, Virginie Pellouin, Marielle Besnard-Gonnet, François Artiguenave, Claudine Médigue, David Vallenet, Antoine Danchin, Anne Zaparucha, Jean Weissenbach, Marcel Salanoubat & Véronique de Berardinis
Autocatalytic backbone N-methylation in a family of ribosomal peptide natural products
June 5th, 2017 by Niels S van der Velden
Nature Chemical Biology 13, 833 (2017). doi:10.1038/nchembio.2393
Authors: Niels S van der Velden, Noemi Kälin, Maximilian J Helf, Jörn Piel, Michael F Freeman & Markus Künzler
Peptide backbone N-methylation, as seen in cyclosporin A, has been considered to be exclusive to nonribosomal peptides. We have identified the first post-translationally modified peptide or protein harboring internal α-N-methylations through discovery of the genetic locus for the omphalotins, cyclic N-methylated peptides produced by the fungus Omphalotus olearius. We show that iterative autocatalytic activity of an N-methyltransferase fused to its peptide substrate is the signature of a new family of ribosomally encoded metabolites.
Blocking an N-terminal acetylation–dependent protein interaction inhibits an E3 ligase
June 5th, 2017 by Daniel C Scott
Nature Chemical Biology 13, 850 (2017). doi:10.1038/nchembio.2386
Authors: Daniel C Scott, Jared T Hammill, Jaeki Min, David Y Rhee, Michele Connelly, Vladislav O Sviderskiy, Deepak Bhasin, Yizhe Chen, Su-Sien Ong, Sergio C Chai, Asli N Goktug, Guochang Huang, Julie K Monda, Jonathan Low, Ho Shin Kim, Joao A Paulo, Joe R Cannon, Anang A Shelat, Taosheng Chen, Ian R Kelsall, Arno F Alpi, Vishwajeeth Pagala, Xusheng Wang, Junmin Peng, Bhuvanesh Singh, J Wade Harper, Brenda A Schulman & R Kip Guy
Functional selectivity of GPCR-directed drug action through location bias
May 29th, 2017 by Roshanak Irannejad
Nature Chemical Biology 13, 799 (2017). doi:10.1038/nchembio.2389
Authors: Roshanak Irannejad, Veronica Pessino, Delphine Mika, Bo Huang, Philip B Wedegaertner, Marco Conti & Mark von Zastrow
Targeting S-adenosylmethionine biosynthesis with a novel allosteric inhibitor of Mat2A
May 29th, 2017 by Casey L Quinlan
Nature Chemical Biology 13, 785 (2017). doi:10.1038/nchembio.2384
Authors: Casey L Quinlan, Stephen E Kaiser, Ben Bolaños, Dawn Nowlin, Rita Grantner, Shannon Karlicek-Bryant, Jun Li Feng, Stephen Jenkinson, Kevin Freeman-Cook, Stephen G Dann, Xiaoli Wang, Peter A Wells, Valeria R Fantin, Al E Stewart & Stephan K Grant
Structural basis for high-affinity fluorophore binding and activation by RNA Mango
May 29th, 2017 by Robert J Trachman
Nature Chemical Biology 13, 807 (2017). doi:10.1038/nchembio.2392
Authors: Robert J Trachman, Natalia A Demeshkina, Matthew W L Lau, Shanker Shyam S Panchapakesan, Sunny C Y Jeng, Peter J Unrau & Adrian R Ferré-D'Amaré
Lipid II overproduction allows direct assay of transpeptidase inhibition by β-lactams
May 29th, 2017 by Yuan Qiao
Nature Chemical Biology 13, 793 (2017). doi:10.1038/nchembio.2388
Authors: Yuan Qiao, Veerasak Srisuknimit, Frederick Rubino, Kaitlin Schaefer, Natividad Ruiz, Suzanne Walker & Daniel Kahne
Structural and functional characterization of the hydrogenase-maturation HydF protein
May 29th, 2017 by Giorgio Caserta
Nature Chemical Biology 13, 779 (2017). doi:10.1038/nchembio.2385
Authors: Giorgio Caserta, Ludovic Pecqueur, Agnieszka Adamska-Venkatesh, Cecilia Papini, Souvik Roy, Vincent Artero, Mohamed Atta, Edward Reijerse, Wolfgang Lubitz & Marc Fontecave