The Rrp4–exosome complex recruits and channels substrate RNA by a unique mechanism

March 13th, 2017 by Milos A Cvetkovic

Nature Chemical Biology 13, 522 (2017). doi:10.1038/nchembio.2328

Authors: Milos A Cvetkovic, Jan Philip Wurm, Maxime J Audin, Stefan Schütz & Remco Sprangers

  • Posted in Nat Chem Biol, Publications
  • Comments Off on The Rrp4–exosome complex recruits and channels substrate RNA by a unique mechanism

In silico design of novel probes for the atypical opioid receptor MRGPRX2

March 13th, 2017 by Katherine Lansu

Nature Chemical Biology 13, 529 (2017). doi:10.1038/nchembio.2334

Authors: Katherine Lansu, Joel Karpiak, Jing Liu, Xi-Ping Huang, John D McCorvy, Wesley K Kroeze, Tao Che, Hiroshi Nagase, Frank I Carroll, Jian Jin, Brian K Shoichet & Bryan L Roth

  • Posted in Nat Chem Biol, Publications
  • Comments Off on In silico design of novel probes for the atypical opioid receptor MRGPRX2

Decoding cyclase-dependent assembly of hapalindole and fischerindole alkaloids

March 13th, 2017 by Shasha Li

Nature Chemical Biology 13, 467 (2017). doi:10.1038/nchembio.2327

Authors: Shasha Li, Andrew N Lowell, Sean A Newmister, Fengan Yu, Robert M Williams & David H Sherman

The formation of C–C bonds in an enantioselective fashion to create complex polycyclic scaffolds in the hapalindole- and fischerindole- type alkaloids from Stigonematales cyanobacteria represents a compelling and urgent challenge in adapting microbial biosynthesis as a catalytic platform in drug development. Here we determine the biochemical basis for tri- and tetracyclic core formation in these secondary metabolites, involving a new class of cyclases that catalyze a complex cyclization cascade.

  • Posted in Nat Chem Biol, Publications
  • Comments Off on Decoding cyclase-dependent assembly of hapalindole and fischerindole alkaloids

Structural basis of PROTAC cooperative recognition for selective protein degradation

March 13th, 2017 by Morgan S Gadd

Nature Chemical Biology 13, 514 (2017). doi:10.1038/nchembio.2329

Authors: Morgan S Gadd, Andrea Testa, Xavier Lucas, Kwok-Ho Chan, Wenzhang Chen, Douglas J Lamont, Michael Zengerle & Alessio Ciulli

  • Posted in Nat Chem Biol, Publications
  • Comments Off on Structural basis of PROTAC cooperative recognition for selective protein degradation

L-2-Hydroxyglutarate production arises from noncanonical enzyme function at acidic pH

March 6th, 2017 by Andrew M Intlekofer

Nature Chemical Biology 13, 494 (2017). doi:10.1038/nchembio.2307

Authors: Andrew M Intlekofer, Bo Wang, Hui Liu, Hardik Shah, Carlos Carmona-Fontaine, Ariën S Rustenburg, Salah Salah, M R Gunner, John D Chodera, Justin R Cross & Craig B Thompson

  • Posted in Nat Chem Biol, Publications
  • Comments Off on L-2-Hydroxyglutarate production arises from noncanonical enzyme function at acidic pH

The structure of a nucleolytic ribozyme that employs a catalytic metal ion

March 6th, 2017 by Yijin Liu

Nature Chemical Biology 13, 508 (2017). doi:10.1038/nchembio.2333

Authors: Yijin Liu, Timothy J Wilson & David M J Lilley

  • Posted in Nat Chem Biol, Publications
  • Comments Off on The structure of a nucleolytic ribozyme that employs a catalytic metal ion

Total RNA-seq to identify pharmacological effects on specific stages of mRNA synthesis

March 6th, 2017 by Sarah A Boswell

Nature Chemical Biology 13, 501 (2017). doi:10.1038/nchembio.2317

Authors: Sarah A Boswell, Andrew Snavely, Heather M Landry, L Stirling Churchman, Jesse M Gray & Michael Springer

  • Posted in Nat Chem Biol, Publications
  • Comments Off on Total RNA-seq to identify pharmacological effects on specific stages of mRNA synthesis

Capzimin is a potent and specific inhibitor of proteasome isopeptidase Rpn11

February 28th, 2017 by Jing Li

Nature Chemical Biology 13, 486 (2017). doi:10.1038/nchembio.2326

Authors: Jing Li, Tanya Yakushi, Francesco Parlati, Andrew L Mackinnon, Christian Perez, Yuyong Ma, Kyle P Carter, Sharon Colayco, Gavin Magnuson, Brock Brown, Kevin Nguyen, Stefan Vasile, Eigo Suyama, Layton H Smith, Eduard Sergienko, Anthony B Pinkerton, Thomas D Y Chung, Amy E Palmer, Ian Pass, Sonja Hess, Seth M Cohen & Raymond J Deshaies

  • Posted in Nat Chem Biol, Publications
  • Comments Off on Capzimin is a potent and specific inhibitor of proteasome isopeptidase Rpn11

A fully automated flow-based approach for accelerated peptide synthesis

February 28th, 2017 by Alexander J Mijalis

Nature Chemical Biology 13, 464 (2017). doi:10.1038/nchembio.2318

Authors: Alexander J Mijalis, Dale A Thomas, Mark D Simon, Andrea Adamo, Ryan Beaumont, Klavs F Jensen & Bradley L Pentelute

Here we report a fully automated, flow-based approach to solid-phase polypeptide synthesis, with amide bond formation in 7 seconds and total synthesis times of 40 seconds per amino acid residue. Crude peptide purities and isolated yields were comparable to those for standard-batch solid-phase peptide synthesis. At full capacity, this approach can yield tens of thousands of individual 30-mer peptides per year.

  • Posted in Nat Chem Biol, Publications
  • Comments Off on A fully automated flow-based approach for accelerated peptide synthesis

A new genome-mining tool redefines the lasso peptide biosynthetic landscape

February 28th, 2017 by Jonathan I Tietz

Nature Chemical Biology 13, 470 (2017). doi:10.1038/nchembio.2319

Authors: Jonathan I Tietz, Christopher J Schwalen, Parth S Patel, Tucker Maxson, Patricia M Blair, Hua-Chia Tai, Uzma I Zakai & Douglas A Mitchell

  • Posted in Nat Chem Biol, Publications
  • Comments Off on A new genome-mining tool redefines the lasso peptide biosynthetic landscape