Engineering RGB color vision into Escherichia coli

May 22nd, 2017 by Jesus Fernandez-Rodriguez

Nature Chemical Biology 13, 706 (2017). doi:10.1038/nchembio.2390

Authors: Jesus Fernandez-Rodriguez, Felix Moser, Miryoung Song & Christopher A Voigt

Optogenetic tools use colored light to rapidly control gene expression in space and time. We designed a genetically encoded system that gives Escherichia coli the ability to distinguish between red, green, and blue (RGB) light and respond by changing gene expression. We use this system to produce 'color photographs' on bacterial culture plates by controlling pigment production and to redirect metabolic flux by expressing CRISPRi guide RNAs.

Engineering protein stability with atomic precision in a monomeric miniprotein

May 22nd, 2017 by Emily G Baker

Nature Chemical Biology 13, 764 (2017). doi:10.1038/nchembio.2380

Authors: Emily G Baker, Christopher Williams, Kieran L Hudson, Gail J Bartlett, Jack W Heal, Kathryn L Porter Goff, Richard B Sessions, Matthew P Crump & Derek N Woolfson

  • Posted in Nat Chem Biol, Publications
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A combinatorial screen of the CLOUD uncovers a synergy targeting the androgen receptor

May 22nd, 2017 by Marco P Licciardello

Nature Chemical Biology 13, 771 (2017). doi:10.1038/nchembio.2382

Authors: Marco P Licciardello, Anna Ringler, Patrick Markt, Freya Klepsch, Charles-Hugues Lardeau, Sara Sdelci, Erika Schirghuber, André C Müller, Michael Caldera, Anja Wagner, Rebecca Herzog, Thomas Penz, Michael Schuster, Bernd Boidol, Gerhard Dürnberger, Yasin Folkvaljon, Pär Stattin, Vladimir Ivanov, Jacques Colinge, Christoph Bock, Klaus Kratochwill, Jörg Menche, Keiryn L Bennett & Stefan Kubicek

  • Posted in Nat Chem Biol, Publications
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Recognition of EGF-like domains by the Notch-modifying O-fucosyltransferase POFUT1

May 22nd, 2017 by Zhijie Li

Nature Chemical Biology 13, 757 (2017). doi:10.1038/nchembio.2381

Authors: Zhijie Li, Kristina Han, John E Pak, Malathy Satkunarajah, Dongxia Zhou & James M Rini

  • Posted in Nat Chem Biol, Publications
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Plant development: Get lit on steroids

May 17th, 2017 by Mirella Bucci

Nature Chemical Biology 13, 569 (2017). doi:10.1038/nchembio.2401

Author: Mirella Bucci

Signal Transduction: Notch catches a Jagged edge

May 17th, 2017 by Stephen C Blacklow

Nature Chemical Biology 13, 570 (2017). doi:10.1038/nchembio.2379

Author: Stephen C Blacklow

Notch signaling is an essential cell–cell communication pathway that influences numerous cell fate decisions during development. Structural and biochemical studies of a Notch–Jagged complex dramatically advance current understanding of ligand recognition, and reveal evidence of catch-bond behavior in the complex.

Erratum: A Vibrio cholerae autoinducer–receptor pair that controls biofilm formation

May 17th, 2017 by Kai Papenfort

Nature Chemical Biology 13, 691 (2017). doi:10.1038/nchembio0617-691a

Author: Kai Papenfort, Justin E Silpe, Kelsey R Schramma, Jian-Ping Cong, Mohammad R Seyedsayamdost & Bonnie L Bassler

  • Posted in Nat Chem Biol, Publications
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Optogenetics: Switching with red and blue

May 17th, 2017 by Fuun Kawano

Nature Chemical Biology 13, 573 (2017). doi:10.1038/nchembio.2387

Authors: Fuun Kawano, Fan Shi & Masayuki Yazawa

Multiple optogenetic technologies are required to control biological activity simultaneously with different colors of light. Optimizing a near-infrared-induced heterodimerization system, which can be combined with blue-light-controlled domains, enables precise spatiotemporal control of target molecules in live mammalian cells.

Small-molecule pheromones and hormones controlling nematode development

May 17th, 2017 by Rebecca A Butcher

Nature Chemical Biology 13, 577 (2017). doi:10.1038/nchembio.2356

Author: Rebecca A Butcher

  • Posted in Nat Chem Biol, Publications
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Erratum: Full antagonism of the estrogen receptor without a prototypical ligand side chain

May 17th, 2017 by Sathish Srinivasan

Nature Chemical Biology 13, 691 (2017). doi:10.1038/nchembio0617-691b

Author: Sathish Srinivasan, Jerome C Nwachukwu, Nelson E Bruno, Venkatasubramanian Dharmarajan, Devrishi Goswami, Irida Kastrati, Scott Novick, Jason Nowak, Valerie Cavett, Hai-Bing Zhou, Nittaya Boonmuen, Yuechao Zhao, Jian Min, Jonna Frasor, Benita S Katzenellenbogen, Patrick R Griffin, John A Katzenellenbogen & Kendall W Nettles

  • Posted in Nat Chem Biol, Publications
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