The structural organization of substrate loading in iterative polyketide synthases

April 2nd, 2018 by Dominik A. Herbst

The structural organization of substrate loading in iterative polyketide synthases

The structural organization of substrate loading in iterative polyketide synthases, Published online: 02 April 2018; doi:10.1038/s41589-018-0026-3

The crystal structure and cryo-electron microscopy of the loading/condensing region of a nonreducing polyketide synthase reveals the insertion of a starter-unit acyltransferase into the condensing region and an asymmetrical post-loading state.
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Author Correction: Mitochondrial DNA repair and replication proteins revealed by targeted chemical probes

April 2nd, 2018 by Simon Wisnovsky

Author Correction: Mitochondrial DNA repair and replication proteins revealed by targeted chemical probes

Author Correction: Mitochondrial DNA repair and replication proteins revealed by targeted chemical probes, Published online: 02 April 2018; doi:10.1038/s41589-018-0040-5

Author Correction: Mitochondrial DNA repair and replication proteins revealed by targeted chemical probes
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The dTAG system for immediate and target-specific protein degradation

March 26th, 2018 by Behnam Nabet

The dTAG system for immediate and target-specific protein degradation

The dTAG system for immediate and target-specific protein degradation, Published online: 26 March 2018; doi:10.1038/s41589-018-0021-8

The dTAG system pairs potent heterobifunctional degraders and extensible tagging strategies to achieve immediate and reversible degradation of divergent proteins, facilitating biological investigation and drug target validation in cells and in mice.
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TAGing for destruction

March 26th, 2018 by Aisha Yesbolatova

TAGing for destruction

TAGing for destruction, Published online: 26 March 2018; doi:10.1038/s41589-018-0024-5

Proteins brought to an E3 ubiquitin ligase are polyubiquitylated for rapid degradation via the proteasome. A new study developed a tag-based degradation system using heterobifunctional degradation inducers called dTAGs, which rapidly depleted FKBP12F36V-fused proteins in cultured cells and in mice.

Unfolding of a ClC chloride transporter retains memory of its evolutionary history

March 26th, 2018 by Duyoung Min

Unfolding of a ClC chloride transporter retains memory of its evolutionary history

Unfolding of a ClC chloride transporter retains memory of its evolutionary history, Published online: 26 March 2018; doi:10.1038/s41589-018-0025-4

A single-molecule forced unfolding of E. coli chloride transporter ClC-ec1 shows that the N- and C-terminal halves of the protein unfold independently, with exposed polar surfaces stabilized by membrane lipid head groups and water.
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Biosynthesis of redox-active metabolites in response to iron deficiency in plants

March 26th, 2018 by Jakub Rajniak

Biosynthesis of redox-active metabolites in response to iron deficiency in plants

Biosynthesis of redox-active metabolites in response to iron deficiency in plants, Published online: 26 March 2018; doi:10.1038/s41589-018-0019-2

The biosynthesis and secretion of redox-active coumarins sideretin and fraxetin in Arabidopsis thaliana enables the plant to acquire iron under nutrient-limited conditions and provides a blueprint for the use of related compounds in other eudicots.
  • Posted in Nat Chem Biol, Publications
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A ‘cis’ted localization

March 19th, 2018 by Mirella Bucci

A ‘cis’ted localization

A ‘cis’ted localization, Published online: 19 March 2018; doi:10.1038/s41589-018-0029-0

A ‘cis’ted localization

RaPID hookup

March 19th, 2018 by Grant Miura

RaPID hookup

RaPID hookup, Published online: 19 March 2018; doi:10.1038/s41589-018-0030-7

RaPID hookup

An optically controlled probe identifies lipid-gating fenestrations within the TRPC3 channel

March 19th, 2018 by Michaela Lichtenegger

An optically controlled probe identifies lipid-gating fenestrations within the TRPC3 channel

An optically controlled probe identifies lipid-gating fenestrations within the TRPC3 channel, Published online: 19 March 2018; doi:10.1038/s41589-018-0015-6

A photoswitchable diacylglycerol enabled a screen that found critical TRPC3 lipid-sensing residues and identified a lateral fenestration in the pore domain that allows lipids to protrude into the permeation pathway to control channel gating.
  • Posted in Nat Chem Biol, Publications
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Specialized splicing

March 19th, 2018 by Caitlin Deane

Specialized splicing

Specialized splicing, Published online: 19 March 2018; doi:10.1038/s41589-018-0027-2

Specialized splicing