Single-molecule analysis reveals agonist-specific dimer formation of µ-opioid receptors

June 15th, 2020 by Jan Möller

Nature Chemical Biology, Published online: 15 June 2020; doi:10.1038/s41589-020-0566-1

Single-molecule and super-resolution approaches define a monomer–dimer equilibrium of µ-opioid receptors and show that receptors form agonist-induced dimers coincident with β-arrestin2 binding to receptors.
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In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design

June 15th, 2020 by Ashty S. Karim

Nature Chemical Biology, Published online: 15 June 2020; doi:10.1038/s41589-020-0559-0

The iPROBE platform accelerates the design and optimization of engineered biosynthetic pathways using a combination of cell-free protein synthesis, in vitro pathway assembly and a scoring system to identify high-performing combinations.
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Author Correction: Higher-order epistasis shapes the fitness landscape of a xenobiotic-degrading enzyme

June 12th, 2020 by Gloria Yang

Nature Chemical Biology, Published online: 12 June 2020; doi:10.1038/s41589-020-0588-8

Author Correction: Higher-order epistasis shapes the fitness landscape of a xenobiotic-degrading enzyme
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Discovery of small-molecule enzyme activators by activity-based protein profiling

June 8th, 2020 by Bernard P. Kok

Nature Chemical Biology, Published online: 08 June 2020; doi:10.1038/s41589-020-0555-4

Activity-based protein profiling (ABPP) was used to identify and optimize bioactive, selective pharmacological enzyme activators of the serine hydrolase LYPLAL1, which improved the metabolic defects of diet-induced obese mice.
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Use of paramagnetic <sup>19</sup>F NMR to monitor domain movement in a glutamate transporter homolog

June 8th, 2020 by Yun Huang

Nature Chemical Biology, Published online: 08 June 2020; doi:10.1038/s41589-020-0561-6

A 19F-NMR-based method monitoring the conformational dynamics of the glutamate transporter GltPh identified one inward- and two outward-facing states, including one unanticipated outward-facing state that was characterized by cryo-EM.
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Comparative tRNA sequencing and RNA mass spectrometry for surveying tRNA modifications

June 8th, 2020 by Satoshi Kimura

Nature Chemical Biology, Published online: 08 June 2020; doi:10.1038/s41589-020-0558-1

A combination of tRNA sequencing and mass spectrometry was developed to profile tRNA modification enabling identification of a new modification acacp3U and the presence of cytosine-to-pseudouracil RNA editing in Vibrio cholerae.
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Rolf Huisgen (1920–2020)

June 2nd, 2020 by Dirk Trauner

Nature Chemical Biology, Published online: 02 June 2020; doi:10.1038/s41589-020-0571-4

Rolf Huisgen left us on 26 March 2020, just three months shy of his 100th birthday. His work has had an enormous influence on chemical biology, ranging from new methods for the synthesis of chemical probes to “click chemistry” and its application to in vivo bioconjugation.

Publisher Correction: Complete reconstitution of the diverse pathways of gentamicin B biosynthesis

June 2nd, 2020 by Yeon Hee Ban

Nature Chemical Biology, Published online: 02 June 2020; doi:10.1038/s41589-020-0577-y

Publisher Correction: Complete reconstitution of the diverse pathways of gentamicin B biosynthesis
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Pyridoxal-5′-phosphate-dependent alkyl transfer in nucleoside antibiotic biosynthesis

June 1st, 2020 by Zheng Cui

Nature Chemical Biology, Published online: 01 June 2020; doi:10.1038/s41589-020-0548-3

Rather than a typical S-adenosylmethionine-dependent alkyltransferase, the installation of the N-alkylamine linker in several nucleoside antibiotics is catalyzed via γ-replacement by a pyridoxal-5′-phosphate-dependent aminobutyryltransferase.
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Selective <i>N</i>-glycan editing on living cell surfaces to probe glycoconjugate function

June 1st, 2020 by Feng Tang

Nature Chemical Biology, Published online: 01 June 2020; doi:10.1038/s41589-020-0551-8

Cellular glycosylation facilitates molecular recognition of cells and biomolecules. A two-step N-glycan editing method enables selective glycoform ‘deletion’ and ‘insertion’ of new glycans, which can be used to probe their biological functions.
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