Site-specific incorporation of phosphotyrosine using an expanded genetic code

June 12th, 2017 by Christian Hoppmann

Nature Chemical Biology 13, 842 (2017). doi:10.1038/nchembio.2406

Authors: Christian Hoppmann, Allison Wong, Bing Yang, Shuwei Li, Tony Hunter, Kevan M Shokat & Lei Wang

Access to phosphoproteins with stoichiometric and site-specific phosphorylation status is key to understanding the role of protein phosphorylation. Here we report an efficient method to generate pure, active phosphotyrosine-containing proteins by genetically encoding a stable phosphotyrosine analog that is convertible to native phosphotyrosine. We demonstrate its general compatibility with proteins of various sizes, phosphotyrosine sites and functions, and reveal a possible role of tyrosine phosphorylation in negative regulation of ubiquitination.

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Blocking an N-terminal acetylation–dependent protein interaction inhibits an E3 ligase

June 5th, 2017 by Daniel C Scott

Nature Chemical Biology 13, 850 (2017). doi:10.1038/nchembio.2386

Authors: Daniel C Scott, Jared T Hammill, Jaeki Min, David Y Rhee, Michele Connelly, Vladislav O Sviderskiy, Deepak Bhasin, Yizhe Chen, Su-Sien Ong, Sergio C Chai, Asli N Goktug, Guochang Huang, Julie K Monda, Jonathan Low, Ho Shin Kim, Joao A Paulo, Joe R Cannon, Anang A Shelat, Taosheng Chen, Ian R Kelsall, Arno F Alpi, Vishwajeeth Pagala, Xusheng Wang, Junmin Peng, Bhuvanesh Singh, J Wade Harper, Brenda A Schulman & R Kip Guy

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Activity-based probes for functional interrogation of retaining β-glucuronidases

June 5th, 2017 by Liang Wu

Nature Chemical Biology 13, 867 (2017). doi:10.1038/nchembio.2395

Authors: Liang Wu, Jianbing Jiang, Yi Jin, Wouter W Kallemeijn, Chi-Lin Kuo, Marta Artola, Wei Dai, Cas van Elk, Marco van Eijk, Gijsbert A van der Marel, Jeroen D C Codée, Bogdan I Florea, Johannes M F G Aerts, Herman S Overkleeft & Gideon J Davies

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Parallel evolution of non-homologous isofunctional enzymes in methionine biosynthesis

June 5th, 2017 by Karine Bastard

Nature Chemical Biology 13, 858 (2017). doi:10.1038/nchembio.2397

Authors: Karine Bastard, Alain Perret, Aline Mariage, Thomas Bessonnet, Agnès Pinet-Turpault, Jean-Louis Petit, Ekaterina Darii, Pascal Bazire, Carine Vergne-Vaxelaire, Clémence Brewee, Adrien Debard, Virginie Pellouin, Marielle Besnard-Gonnet, François Artiguenave, Claudine Médigue, David Vallenet, Antoine Danchin, Anne Zaparucha, Jean Weissenbach, Marcel Salanoubat & Véronique de Berardinis

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Autocatalytic backbone N-methylation in a family of ribosomal peptide natural products

June 5th, 2017 by Niels S van der Velden

Nature Chemical Biology 13, 833 (2017). doi:10.1038/nchembio.2393

Authors: Niels S van der Velden, Noemi Kälin, Maximilian J Helf, Jörn Piel, Michael F Freeman & Markus Künzler

Peptide backbone N-methylation, as seen in cyclosporin A, has been considered to be exclusive to nonribosomal peptides. We have identified the first post-translationally modified peptide or protein harboring internal α-N-methylations through discovery of the genetic locus for the omphalotins, cyclic N-methylated peptides produced by the fungus Omphalotus olearius. We show that iterative autocatalytic activity of an N-methyltransferase fused to its peptide substrate is the signature of a new family of ribosomally encoded metabolites.

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Functional selectivity of GPCR-directed drug action through location bias

May 29th, 2017 by Roshanak Irannejad

Nature Chemical Biology 13, 799 (2017). doi:10.1038/nchembio.2389

Authors: Roshanak Irannejad, Veronica Pessino, Delphine Mika, Bo Huang, Philip B Wedegaertner, Marco Conti & Mark von Zastrow

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Targeting S-adenosylmethionine biosynthesis with a novel allosteric inhibitor of Mat2A

May 29th, 2017 by Casey L Quinlan

Nature Chemical Biology 13, 785 (2017). doi:10.1038/nchembio.2384

Authors: Casey L Quinlan, Stephen E Kaiser, Ben Bolaños, Dawn Nowlin, Rita Grantner, Shannon Karlicek-Bryant, Jun Li Feng, Stephen Jenkinson, Kevin Freeman-Cook, Stephen G Dann, Xiaoli Wang, Peter A Wells, Valeria R Fantin, Al E Stewart & Stephan K Grant

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Structural basis for high-affinity fluorophore binding and activation by RNA Mango

May 29th, 2017 by Robert J Trachman

Nature Chemical Biology 13, 807 (2017). doi:10.1038/nchembio.2392

Authors: Robert J Trachman, Natalia A Demeshkina, Matthew W L Lau, Shanker Shyam S Panchapakesan, Sunny C Y Jeng, Peter J Unrau & Adrian R Ferré-D'Amaré

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Lipid II overproduction allows direct assay of transpeptidase inhibition by β-lactams

May 29th, 2017 by Yuan Qiao

Nature Chemical Biology 13, 793 (2017). doi:10.1038/nchembio.2388

Authors: Yuan Qiao, Veerasak Srisuknimit, Frederick Rubino, Kaitlin Schaefer, Natividad Ruiz, Suzanne Walker & Daniel Kahne

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Structural and functional characterization of the hydrogenase-maturation HydF protein

May 29th, 2017 by Giorgio Caserta

Nature Chemical Biology 13, 779 (2017). doi:10.1038/nchembio.2385

Authors: Giorgio Caserta, Ludovic Pecqueur, Agnieszka Adamska-Venkatesh, Cecilia Papini, Souvik Roy, Vincent Artero, Mohamed Atta, Edward Reijerse, Wolfgang Lubitz & Marc Fontecave

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