Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases
May 7th, 2018 by Weston Kightlinger
Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases
Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases, Published online: 07 May 2018; doi:10.1038/s41589-018-0051-2
The GlycoSCORES method, which involves cell-free protein expression and substrate-site profiling of glycosyltransferase enzymes by SAMDI–MS, enables the identification of glycosylation tags for glycoengineering efforts.Publisher Correction: A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cells
May 4th, 2018 by Aaron H. Nile
Publisher Correction: A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cells
Publisher Correction: A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cells, Published online: 04 May 2018; doi:10.1038/s41589-018-0069-5
Publisher Correction: A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cellsPublisher Correction: Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET
May 4th, 2018 by Daria M. Shcherbakova
Publisher Correction: Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET
Publisher Correction: Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET, Published online: 04 May 2018; doi:10.1038/s41589-018-0070-z
Publisher Correction: Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRETShared strategies for β-lactam catabolism in the soil microbiome
April 30th, 2018 by Terence S. Crofts
Shared strategies for β-lactam catabolism in the soil microbiome
Shared strategies for β-lactam catabolism in the soil microbiome, Published online: 30 April 2018; doi:10.1038/s41589-018-0052-1
A β-lactamase, a novel type of amidase, and the phenylacetic acid catabolon comprise a catabolic pathway, revealed by genomic and transcriptomic analysis, that enables multiple soil bacteria to use β-lactam antibiotics as a carbon source.Evolution of cyclohexadienyl dehydratase from an ancestral solute-binding protein
April 23rd, 2018 by Ben E. Clifton
Evolution of cyclohexadienyl dehydratase from an ancestral solute-binding protein
Evolution of cyclohexadienyl dehydratase from an ancestral solute-binding protein, Published online: 23 April 2018; doi:10.1038/s41589-018-0043-2
Ancestral protein reconstruction, with structural and biochemical analysis, illustrates the evolution of a solute-binding protein to cyclohexadienyl dehydratase through incorporation of a catalytic residue and gradual reshaping of the binding site.Generalized extracellular molecule sensor platform for programming cellular behavior
April 23rd, 2018 by Leo Scheller
Generalized extracellular molecule sensor platform for programming cellular behavior
Generalized extracellular molecule sensor platform for programming cellular behavior, Published online: 23 April 2018; doi:10.1038/s41589-018-0046-z
Engineered erythropoietin receptor scaffolds equipped with extracellular sensor domains and modular intracellular domains that couple to endogenous signaling pathways enable modular reprogramming of designer membrane-bound receptors.Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET
April 23rd, 2018 by Daria M. Shcherbakova
Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET
Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET, Published online: 23 April 2018; doi:10.1038/s41589-018-0044-1
The monomeric near-infrared (NIR) fluorescent protein miRFP720 enables development of fully NIR Förster resonance energy transfer (FRET) biosensors compatible with CFP–YFP FRET biosensors and blue–green optogenetic tools without optical cross-talk.Evolution of chalcone isomerase from a noncatalytic ancestor
April 23rd, 2018 by Miriam Kaltenbach
Evolution of chalcone isomerase from a noncatalytic ancestor
Evolution of chalcone isomerase from a noncatalytic ancestor, Published online: 23 April 2018; doi:10.1038/s41589-018-0042-3
Ancestral sequence inference, directed evolution, structural analysis, NMR, and molecular dynamics simulations illuminate how enantioselective activity arises during the evolutionary trajectory of chalcone isomerase from a noncatalytic ancestor.Understanding the Pro/N-end rule pathway
April 16th, 2018 by David A. Dougan
Understanding the Pro/N-end rule pathway
Understanding the Pro/N-end rule pathway, Published online: 16 April 2018; doi:10.1038/s41589-018-0045-0
Regulated destruction of proteins underlies just about everything a cell does. A structural study of human Gid4, the N-recognin of the Pro/N-end rule pathway that targets proteins through their N-terminal proline, illuminates the recognition of substrates by this proteolytic system.Spotting the signal
April 16th, 2018 by Caitlin Deane
Spotting the signal
Spotting the signal, Published online: 16 April 2018; doi:10.1038/s41589-018-0047-y
Spotting the signal