Nuclear RNR-α antagonizes cell proliferation by directly inhibiting ZRANB3

August 27th, 2018 by Yuan Fu

Nuclear RNR-α antagonizes cell proliferation by directly inhibiting ZRANB3

Nuclear RNR-α antagonizes cell proliferation by directly inhibiting ZRANB3, Published online: 27 August 2018; doi:10.1038/s41589-018-0113-5

The large subunit of ribonucleotide reductase RNR-α downregulates DNA replication in the nucleus by directly disrupting PCNA and ZRANB3 interactions. RNR-α nuclear entry is regulated by an interplay between IRBIT and importin-α1.
  • Posted in Nat Chem Biol, Publications
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Acetate-dependent tRNA acetylation required for decoding fidelity in protein synthesis

August 27th, 2018 by Takaaki Taniguchi

Acetate-dependent tRNA acetylation required for decoding fidelity in protein synthesis

Acetate-dependent tRNA acetylation required for decoding fidelity in protein synthesis, Published online: 27 August 2018; doi:10.1038/s41589-018-0119-z

A comparative genomic approach identified a novel acetate-dependent tRNA-modifying enzyme that catalyzes RNA acetylation with a mechanism similar to tRNA aminoacylation. This modification maintains decoding fidelity in protein synthesis.
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<i>O</i>-GlcNAc modification of eIF4GI acts as a translational switch in heat shock response

August 20th, 2018 by Xingqian Zhang

O-GlcNAc modification of eIF4GI acts as a translational switch in heat shock response

<i>O</i>-GlcNAc modification of eIF4GI acts as a translational switch in heat shock response, Published online: 20 August 2018; doi:10.1038/s41589-018-0120-6

O-GlcNAcylation of translation initiation factor component eIF4GI blocks interactions to poly(A)-binding protein Pab1 to induce disassembly of stress granules, releasing Hsp70-induced mRNAs and leading to translation of protective proteins
  • Posted in Nat Chem Biol, Publications
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Continuous directed evolution of proteins with improved soluble expression

August 20th, 2018 by Tina Wang

Continuous directed evolution of proteins with improved soluble expression

Continuous directed evolution of proteins with improved soluble expression, Published online: 20 August 2018; doi:10.1038/s41589-018-0121-5

Through use of a split-intein pIII, soluble expression phage-assisted continuous evolution (SE-PACE) enables two simultaneous positive selections to rapidly evolve proteins with improved expression while maintaining their desired activities.
  • Posted in Nat Chem Biol, Publications
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Mimicking cross-α amyloids

August 17th, 2018 by Meytal Landau

Mimicking cross-α amyloids

Mimicking cross-α amyloids, Published online: 17 August 2018; doi:10.1038/s41589-018-0118-0

The design of spiraling cross-α amyloid-like structures reveals fascinating supermolecular fibrils of diverse compactness and stability. The small sequence variations governing cross-α self-assembly properties concur with amyloids being basic building blocks of life and natural targets for microbial structural mimicry.

Allosteric mechanisms underlie GPCR signaling to SH3-domain proteins through arrestin

August 17th, 2018 by Fan Yang

Allosteric mechanisms underlie GPCR signaling to SH3-domain proteins through arrestin

Allosteric mechanisms underlie GPCR signaling to SH3-domain proteins through arrestin, Published online: 17 August 2018; doi:10.1038/s41589-018-0115-3

NMR and in vitro reconstitution indicate that GPCRs signal through SH3-containing proteins downstream of β-arrestin 1 proline regions and that arrestin allosterically activates downstream kinases by disrupting their autoinhibitory conformation.
  • Posted in Nat Chem Biol, Publications
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An optical sensor to monitor dynamics of extracellular glycine

August 17th, 2018 by Dmitri A. Rusakov

An optical sensor to monitor dynamics of extracellular glycine

An optical sensor to monitor dynamics of extracellular glycine, Published online: 17 August 2018; doi:10.1038/s41589-018-0123-3

A computational design approach was used to develop a genetically encoded FRET-based optical sensor that is aimed at monitoring extracellular glycine levels in brain tissue with the sensitivity and resolution to discern differences in dendritic spine and shaft environment and concentration dynamics upon afferent stimulation.

Catch and identify your prey

August 17th, 2018 by Karin Kuehnel

Catch and identify your prey

Catch and identify your prey, Published online: 17 August 2018; doi:10.1038/s41589-018-0125-1

Catch and identify your prey

A ribonucleotide trap

August 17th, 2018 by Yiyun Song

A ribonucleotide trap

A ribonucleotide trap, Published online: 17 August 2018; doi:10.1038/s41589-018-0127-z

A ribonucleotide trap

<i>ykkC</i> riboswitches employ an add-on helix to adjust specificity for polyanionic ligands

August 17th, 2018 by Alla Peselis

ykkC riboswitches employ an add-on helix to adjust specificity for polyanionic ligands

<i>ykkC</i> riboswitches employ an add-on helix to adjust specificity for polyanionic ligands, Published online: 17 August 2018; doi:10.1038/s41589-018-0114-4

Structural analysis of PRPP and ppGpp riboswitches reveals that they employ a helical element to create a tunnel for the ligand, whose specificity is determined by the conserved nucleotides forming the tunnel and long-distance contacts.
  • Posted in Nat Chem Biol, Publications
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