Expression system for structural and functional studies of human glycosylation enzymes

December 18th, 2017 by Kelley W Moremen

Expression system for structural and functional studies of human glycosylation enzymes

Expression system for structural and functional studies of human glycosylation enzymes, Published online: 18 December 2017; doi:10.1038/nchembio.2539

A modular protein expression system enables the structural and functional characterization of human glycosyltransferases, glycoside hydrolases and other carbohydrate-modifying enzymes.
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Microbiology: Trapping Rac1

December 12th, 2017 by Karin Kuehnel

Microbiology: Trapping Rac1

Microbiology: Trapping Rac1, Published online: 12 December 2017; doi:10.1038/nchembio.2541

Microbiology: Trapping Rac1

Resistance mechanisms: Watering down a warhead

December 12th, 2017 by Caitlin Deane

Resistance mechanisms: Watering down a warhead

Resistance mechanisms: Watering down a warhead, Published online: 12 December 2017; doi:10.1038/nchembio.2542

Resistance mechanisms: Watering down a warhead

Protein engineering: Finding the best ligase

December 12th, 2017 by Christian F W Becker

Protein engineering: Finding the best ligase

Protein engineering: Finding the best ligase, Published online: 12 December 2017; doi:10.1038/nchembio.2533

Modification of folded proteins at will, within any sequence context, remains an elusive goal. A proteome-wide screening approach has now identified a set of protein ligases that enables conjugation of peptides to almost any protein N terminus, overcoming longstanding limitations in protein engineering.

Neurobiology: Defining your territory

December 12th, 2017 by Grant Miura

Neurobiology: Defining your territory

Neurobiology: Defining your territory, Published online: 12 December 2017; doi:10.1038/nchembio.2544

Neurobiology: Defining your territory

RNA modifications: Ribosomes get decorated

December 12th, 2017 by Mirella Bucci

RNA modifications: Ribosomes get decorated

RNA modifications: Ribosomes get decorated, Published online: 12 December 2017; doi:10.1038/nchembio.2543

RNA modifications: Ribosomes get decorated

Notch signaling: A sweet strategy

December 12th, 2017 by Tetsuya Okajima

Notch signaling: A sweet strategy

Notch signaling: A sweet strategy, Published online: 12 December 2017; doi:10.1038/nchembio.2532

Glycosylation of Notch receptors regulates ligand-induced Notch signaling, which is essential for normal development in animals. Fucose analogs targeting Notch glycosylation serve as ligand-specific Notch inhibitors and facilitate the understanding of how O-glycan regulates Notch–ligand interactions.

Structure-inspired design of β-arrestin-biased ligands for aminergic GPCRs

December 11th, 2017 by John D McCorvy

Structure-inspired design of β-arrestin-biased ligands for aminergic GPCRs

Structure-inspired design of β-arrestin-biased ligands for aminergic GPCRs, Published online: 11 December 2017; doi:10.1038/nchembio.2527

Development of D2 dopamine receptor ligands biased for β-arrestin recruitment based on a receptor homology model that identified conserved ligand contacts within the TM5 and EL2 regions as important for biased signaling.
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Engineered synthetic scaffolds for organizing proteins within the bacterial cytoplasm

December 11th, 2017 by Matthew J Lee

Engineered synthetic scaffolds for organizing proteins within the bacterial cytoplasm

Engineered synthetic scaffolds for organizing proteins within the bacterial cytoplasm, Published online: 11 December 2017; doi:10.1038/nchembio.2535

Two complementary coiled-coil peptides and a bacterial microcompartment shell protein are combined to construct cytoscaffolds within Escherichia coli cells. Targeting enzymes to the cytoplasmic scaffold results in colocalization and improved metabolic flux.
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Human antibody-based chemically induced dimerizers for cell therapeutic applications

December 4th, 2017 by Zachary B Hill

Human antibody-based chemically induced dimerizers for cell therapeutic applications

Human antibody-based chemically induced dimerizers for cell therapeutic applications, Published online: 04 December 2017; doi:10.1038/nchembio.2529

Selections with a phage-displayed antibody library against an existing protein–small-molecule complex enabled the generation of antibody-based chemically induced dimerizers (AbCIDs) with the properties necessary for use in regulating cell therapies.
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