Site-specific incorporation of phosphotyrosine using an expanded genetic code

June 12th, 2017 by Christian Hoppmann

Nature Chemical Biology 13, 842 (2017). doi:10.1038/nchembio.2406

Authors: Christian Hoppmann, Allison Wong, Bing Yang, Shuwei Li, Tony Hunter, Kevan M Shokat & Lei Wang

Access to phosphoproteins with stoichiometric and site-specific phosphorylation status is key to understanding the role of protein phosphorylation. Here we report an efficient method to generate pure, active phosphotyrosine-containing proteins by genetically encoding a stable phosphotyrosine analog that is convertible to native phosphotyrosine. We demonstrate its general compatibility with proteins of various sizes, phosphotyrosine sites and functions, and reveal a possible role of tyrosine phosphorylation in negative regulation of ubiquitination.

  • Posted in Nat Chem Biol, Publications
  • Comments Off on Site-specific incorporation of phosphotyrosine using an expanded genetic code

Oxidation of phosphorothioate DNA modifications leads to lethal genomic instability

June 12th, 2017 by Stefanie Kellner

Nature Chemical Biology 13, 888 (2017). doi:10.1038/nchembio.2407

Authors: Stefanie Kellner, Michael S DeMott, Ching Pin Cheng, Brandon S Russell, Bo Cao, Delin You & Peter C Dedon

  • Posted in Nat Chem Biol, Publications
  • Comments Off on Oxidation of phosphorothioate DNA modifications leads to lethal genomic instability

Genetically encoding phosphotyrosine and its nonhydrolyzable analog in bacteria

June 12th, 2017 by Xiaozhou Luo

Nature Chemical Biology 13, 845 (2017). doi:10.1038/nchembio.2405

Authors: Xiaozhou Luo, Guangsen Fu, Rongsheng E Wang, Xueyong Zhu, Claudio Zambaldo, Renhe Liu, Tao Liu, Xiaoxuan Lyu, Jintang Du, Weimin Xuan, Anzhi Yao, Sean A Reed, Mingchao Kang, Yuhan Zhang, Hui Guo, Chunhui Huang, Peng-Yu Yang, Ian A Wilson, Peter G Schultz & Feng Wang

  • Posted in Nat Chem Biol, Publications
  • Comments Off on Genetically encoding phosphotyrosine and its nonhydrolyzable analog in bacteria

A mutant O-GlcNAcase enriches Drosophila developmental regulators

June 12th, 2017 by Nithya Selvan

Nature Chemical Biology 13, 882 (2017). doi:10.1038/nchembio.2404

Authors: Nithya Selvan, Ritchie Williamson, Daniel Mariappa, David G Campbell, Robert Gourlay, Andrew T Ferenbach, Tonia Aristotelous, Iva Hopkins-Navratilova, Matthias Trost & Daan M F van Aalten

Blocking an N-terminal acetylation–dependent protein interaction inhibits an E3 ligase

June 5th, 2017 by Daniel C Scott

Nature Chemical Biology 13, 850 (2017). doi:10.1038/nchembio.2386

Authors: Daniel C Scott, Jared T Hammill, Jaeki Min, David Y Rhee, Michele Connelly, Vladislav O Sviderskiy, Deepak Bhasin, Yizhe Chen, Su-Sien Ong, Sergio C Chai, Asli N Goktug, Guochang Huang, Julie K Monda, Jonathan Low, Ho Shin Kim, Joao A Paulo, Joe R Cannon, Anang A Shelat, Taosheng Chen, Ian R Kelsall, Arno F Alpi, Vishwajeeth Pagala, Xusheng Wang, Junmin Peng, Bhuvanesh Singh, J Wade Harper, Brenda A Schulman & R Kip Guy

  • Posted in Nat Chem Biol, Publications
  • Comments Off on Blocking an N-terminal acetylation–dependent protein interaction inhibits an E3 ligase

Activity-based probes for functional interrogation of retaining β-glucuronidases

June 5th, 2017 by Liang Wu

Nature Chemical Biology 13, 867 (2017). doi:10.1038/nchembio.2395

Authors: Liang Wu, Jianbing Jiang, Yi Jin, Wouter W Kallemeijn, Chi-Lin Kuo, Marta Artola, Wei Dai, Cas van Elk, Marco van Eijk, Gijsbert A van der Marel, Jeroen D C Codée, Bogdan I Florea, Johannes M F G Aerts, Herman S Overkleeft & Gideon J Davies

  • Posted in Nat Chem Biol, Publications
  • Comments Off on Activity-based probes for functional interrogation of retaining β-glucuronidases

Parallel evolution of non-homologous isofunctional enzymes in methionine biosynthesis

June 5th, 2017 by Karine Bastard

Nature Chemical Biology 13, 858 (2017). doi:10.1038/nchembio.2397

Authors: Karine Bastard, Alain Perret, Aline Mariage, Thomas Bessonnet, Agnès Pinet-Turpault, Jean-Louis Petit, Ekaterina Darii, Pascal Bazire, Carine Vergne-Vaxelaire, Clémence Brewee, Adrien Debard, Virginie Pellouin, Marielle Besnard-Gonnet, François Artiguenave, Claudine Médigue, David Vallenet, Antoine Danchin, Anne Zaparucha, Jean Weissenbach, Marcel Salanoubat & Véronique de Berardinis

  • Posted in Nat Chem Biol, Publications
  • Comments Off on Parallel evolution of non-homologous isofunctional enzymes in methionine biosynthesis

Autocatalytic backbone N-methylation in a family of ribosomal peptide natural products

June 5th, 2017 by Niels S van der Velden

Nature Chemical Biology 13, 833 (2017). doi:10.1038/nchembio.2393

Authors: Niels S van der Velden, Noemi Kälin, Maximilian J Helf, Jörn Piel, Michael F Freeman & Markus Künzler

Peptide backbone N-methylation, as seen in cyclosporin A, has been considered to be exclusive to nonribosomal peptides. We have identified the first post-translationally modified peptide or protein harboring internal α-N-methylations through discovery of the genetic locus for the omphalotins, cyclic N-methylated peptides produced by the fungus Omphalotus olearius. We show that iterative autocatalytic activity of an N-methyltransferase fused to its peptide substrate is the signature of a new family of ribosomally encoded metabolites.

  • Posted in Nat Chem Biol, Publications
  • Comments Off on Autocatalytic backbone N-methylation in a family of ribosomal peptide natural products

Structural and functional characterization of the hydrogenase-maturation HydF protein

May 29th, 2017 by Giorgio Caserta

Nature Chemical Biology 13, 779 (2017). doi:10.1038/nchembio.2385

Authors: Giorgio Caserta, Ludovic Pecqueur, Agnieszka Adamska-Venkatesh, Cecilia Papini, Souvik Roy, Vincent Artero, Mohamed Atta, Edward Reijerse, Wolfgang Lubitz & Marc Fontecave

  • Posted in Nat Chem Biol, Publications
  • Comments Off on Structural and functional characterization of the hydrogenase-maturation HydF protein

Functional selectivity of GPCR-directed drug action through location bias

May 29th, 2017 by Roshanak Irannejad

Nature Chemical Biology 13, 799 (2017). doi:10.1038/nchembio.2389

Authors: Roshanak Irannejad, Veronica Pessino, Delphine Mika, Bo Huang, Philip B Wedegaertner, Marco Conti & Mark von Zastrow

  • Posted in Nat Chem Biol, Publications
  • Comments Off on Functional selectivity of GPCR-directed drug action through location bias