Architecture of the membrane-bound cytochrome <i>c</i> heme lyase CcmF
May 6th, 2021 by Anton Brausemann
Nature Chemical Biology, Published online: 06 May 2021; doi:10.1038/s41589-021-00793-8
The structure of the heme lyase CcmF, an integral membrane protein, reveals a cavity opening toward the extracellular side to receive heme groups from the chaperone CcmE and a surface groove for guiding the substrate protein during heme attachment.Genetically encoded multivalent liquid glycan array displayed on M13 bacteriophage
May 6th, 2021 by Mirat Sojitra
Nature Chemical Biology, Published online: 06 May 2021; doi:10.1038/s41589-021-00788-5
Liquid glycan arrays (LiGAs), presented on M13 bacteriophage surface proteins through bioorthogonal chemistry, link surface glycans to genetic barcodes in phage DNA, enabling lectin–glycan interaction profiling by DNA sequencing.Circularly permuted LOV2 as a modular photoswitch for optogenetic engineering
May 6th, 2021 by Lian He
Nature Chemical Biology, Published online: 06 May 2021; doi:10.1038/s41589-021-00792-9
A set of LOV2 circular permutants (cpLOV2) have been engineered to expand the existing optogenetic toolbox. cpLOV2 enables the design of both ON- and OFF-switches to control cell signaling, gene expression, cell fate and cancer immunotherapy.A molecular trap inside microtubules probes luminal access by soluble proteins
May 3rd, 2021 by Yuta Nihongaki
Nature Chemical Biology, Published online: 03 May 2021; doi:10.1038/s41589-021-00791-w
A chemically inducible technique for trapping cytosolic proteins in microtubules (MTs) in cells reveals that soluble proteins can enter the MT lumen by diffusion, while proteins forming a complex with tubulins can be incorporated at MT plus ends.A stop sign for RAS trafficking
April 29th, 2021 by Tian Qiu
Nature Chemical Biology, Published online: 29 April 2021; doi:10.1038/s41589-021-00789-4
N-Ras lipidation cycling is known to regulate its trafficking and function. A new S-depalmitoylation inhibitor induces N-Ras membrane mislocalization, providing insights into N-Ras function and laying the foundation for a new cancer therapeutic strategy.ABHD17 regulation of plasma membrane palmitoylation and N-Ras-dependent cancer growth
April 29th, 2021 by Jarrett R. Remsberg
Nature Chemical Biology, Published online: 29 April 2021; doi:10.1038/s41589-021-00785-8
ABD957 is a potent and selective inhibitor of the ABHD17 family of depalmitoylases that disrupts N-Ras signaling in human acute myeloid leukemia cells and can synergize with MEK inhibition.Engineering AraC to make it responsive to light instead of arabinose
April 26th, 2021 by Edoardo Romano
Nature Chemical Biology, Published online: 26 April 2021; doi:10.1038/s41589-021-00787-6
Engineering of a blue light-inducible AraC dimerization system in E. coli permits light-dependent, rather than arabinose-based, regulation of gene expression from standard PBAD promoters, enabling temporal and spatial control of bacterial systems.Focal assembly
April 26th, 2021 by Yiyun Song
Nature Chemical Biology, Published online: 26 April 2021; doi:10.1038/s41589-021-00797-4
Focal assemblyGuiding light
April 26th, 2021 by Grant Miura
Nature Chemical Biology, Published online: 26 April 2021; doi:10.1038/s41589-021-00794-7
Guiding lightDesigning stable DNAzymes
April 26th, 2021 by Ross Cloney
Nature Chemical Biology, Published online: 26 April 2021; doi:10.1038/s41589-021-00796-5
Designing stable DNAzymes