Synthetic microbial consortia enable rapid assembly of pure translation machinery

November 13th, 2017 by Fernando Villarreal

Synthetic microbial consortia enable rapid assembly of pure translation machinery

Synthetic microbial consortia enable rapid assembly of pure translation machinery, Published online: 13 November 2017; doi:10.1038/nchembio.2514

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Strains of Escherichia coli, each expressing a subset of the 34 translation machinery proteins, are grown in synthetic microbial consortia to enable the efficient isolation of the full machinery from a single culturing, lysis, and purification procedure.

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Nonimmune cells equipped with T-cell-receptor-like signaling for cancer cell ablation

November 13th, 2017 by Ryosuke Kojima

Nonimmune cells equipped with T-cell-receptor-like signaling for cancer cell ablation

Nonimmune cells equipped with T-cell-receptor-like signaling for cancer cell ablation, Published online: 13 November 2017; doi:10.1038/nchembio.2498

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Engineering of nonimmune cells with a cell-contact sensor and antigen recognition domains enables cell-contact-dependent sensor cell signaling and effector molecule production directed to attack target cells, providing an alternative strategy to chimeric antigen receptor T-cell (CAR-T) technology.

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Top-down characterization of endogenous protein complexes with native proteomics

November 13th, 2017 by Owen S Skinner

Top-down characterization of endogenous protein complexes with native proteomics

Top-down characterization of endogenous protein complexes with native proteomics, Published online: 13 November 2017; doi:10.1038/nchembio.2515

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A multistage tandem mass spectrometry approach enables the application of native proteomics to characterize intact endogenous protein complexes in discovery mode, including covalent modifications as well as noncovalently bound cofactors and ligands.

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Metabolomics-based discovery of a metabolite that enhances oligodendrocyte maturation

November 13th, 2017 by Brittney A Beyer

Metabolomics-based discovery of a metabolite that enhances oligodendrocyte maturation

Metabolomics-based discovery of a metabolite that enhances oligodendrocyte maturation, Published online: 13 November 2017; doi:10.1038/nchembio.2517

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Mass-spectrometry-based metabolomics analysis of oligodendrocyte differentiation led to the identification of an endogenous metabolite, taurine, that enhanced the process of drug-induced OPC differentiation.

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Ultrasensitive optical imaging with lanthanide lumiphores

November 6th, 2017 by Ukrae Cho

Ultrasensitive optical imaging with lanthanide lumiphores

Ultrasensitive optical imaging with lanthanide lumiphores, Published online: 06 November 2017; doi:10.1038/nchembio.2513

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Ultrasensitive imaging of lanthanide chelates is achieved by integrating transreflected illumination, luminescence resonance energy transfer, and time-resolved microscopy.

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Purinyl-cobamide is a native prosthetic group of reductive dehalogenases

November 6th, 2017 by Jun Yan

Purinyl-cobamide is a native prosthetic group of reductive dehalogenases

Purinyl-cobamide is a native prosthetic group of reductive dehalogenases, Published online: 06 November 2017; doi:10.1038/nchembio.2512

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In organohalide-respiring bacteria, reductive dehalogenases cleave carbon–halogen bonds using cobamide prosthetic groups. Desulfitobacterium hafniense uses an unprecedented cobamide variant, which includes unsubstituted purine, for this function.

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Synthetic beta cells for fusion-mediated dynamic insulin secretion

October 30th, 2017 by Zhaowei Chen

Synthetic beta cells for fusion-mediated dynamic insulin secretion

Synthetic beta cells for fusion-mediated dynamic insulin secretion, Published online: 30 October 2017; doi:10.1038/nchembio.2511

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Synthetic beta cells were fabricated through 'vesicles-in-vesicle' liposomal superstructures equipped with glucose-sensing and membrane-fusion machinery, thus enabling sensing of graded glucose levels and secretion of insulin via fusion processes.

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PatB1 is an <i>O</i>-acetyltransferase that decorates secondary cell wall polysaccharides

October 30th, 2017 by David Sychantha

PatB1 is an O-acetyltransferase that decorates secondary cell wall polysaccharides

PatB1 is an <i>O</i>-acetyltransferase that decorates secondary cell wall polysaccharides, Published online: 30 October 2017; doi:10.1038/nchembio.2509

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A chemically synthesized analog of Bacillus cereus secondary cell wall polysaccharide (SCWP) facilitates the identification of PatB1 as a SCWP O-acetyltransferase, and the structure of PatB1 provides insights into its catalytic mechanism.

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Small molecule promotes β-catenin citrullination and inhibits Wnt signaling in cancer

October 30th, 2017 by Yi Qu

Small molecule promotes β-catenin citrullination and inhibits Wnt signaling in cancer

Small molecule promotes β-catenin citrullination and inhibits Wnt signaling in cancer, Published online: 30 October 2017; doi:10.1038/nchembio.2510

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The small molecule nitazoxanide (NTZ) was identified as a Wnt inhibitor by promoting protein citrullination of β-catenin through increased cytosolic calcium and PAD2 protein stabilization. β-catenin citrullination results in proteasomal degradation.

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Electrophilic probes for deciphering substrate recognition by O-GlcNAc transferase

October 23rd, 2017 by Chia-Wei Hu

Electrophilic probes for deciphering substrate recognition by O-GlcNAc transferase

Electrophilic probes for deciphering substrate recognition by O-GlcNAc transferase, Published online: 23 October 2017; doi:10.1038/nchembio.2494

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N-Acetylglucosamine derivatives equipped with electrophilic groups and handles for subsequent chemical tagging are useful probes of substrate recognition by O-GlcNAc transferases and enable the capture of transient protein substrates of these enzymes.

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