An aminoacylation ribozyme evolved from a natural tRNA-sensing T-box riboswitch

March 23rd, 2020 by Satoshi Ishida

Nature Chemical Biology, Published online: 23 March 2020; doi:10.1038/s41589-020-0500-6

The authors develop a ribozyme, Tx2.1, that is capable of aminoacylating tRNA with specificity for the anticodon from directed evolution of a T-box riboswitch. Tx2.1 could be used to charge non-natural amino acids in an in vitro translation system.
  • Posted in Nat Chem Biol, Publications
  • Comments Off on An aminoacylation ribozyme evolved from a natural tRNA-sensing T-box riboswitch

The origin of genomic <i>N</i><sup>6</sup>-methyl-deoxyadenosine in mammalian cells

March 23rd, 2020 by Michael U. Musheev

Nature Chemical Biology, Published online: 23 March 2020; doi:10.1038/s41589-020-0504-2

A metabolic labeling method reveals that genomic N6-methyl-deoxyadenosine in mammalian cell lines originates not from direct methylation in DNA, but from a misincorporation of the metabolite of ribo-N6-methyladenosine.
  • Posted in Nat Chem Biol, Publications
  • Comments Off on The origin of genomic <i>N</i><sup>6</sup>-methyl-deoxyadenosine in mammalian cells

A tell-tale absence

March 20th, 2020 by Caitlin Deane

Nature Chemical Biology, Published online: 20 March 2020; doi:10.1038/s41589-020-0513-1

A tell-tale absence

Tick tock

March 20th, 2020 by Yiyun Song

Nature Chemical Biology, Published online: 20 March 2020; doi:10.1038/s41589-020-0515-z

Tick tock

Unifying principles of bifunctional, proximity-inducing small molecules

March 20th, 2020 by Christopher J. Gerry

Nature Chemical Biology, Published online: 20 March 2020; doi:10.1038/s41589-020-0469-1

This Perspective describes the chemical and biophysical principles common to all bifunctional, proximity-inducing small molecules. It also discusses the underappreciated diversity of their chemical structures and biological mechanisms.
  • Posted in Nat Chem Biol, Publications
  • Comments Off on Unifying principles of bifunctional, proximity-inducing small molecules

Locked on target

March 20th, 2020 by Caitlin Deane

Nature Chemical Biology, Published online: 20 March 2020; doi:10.1038/s41589-020-0512-2

Locked on target

A gut reaction

March 20th, 2020 by Mirella Bucci

Nature Chemical Biology, Published online: 20 March 2020; doi:10.1038/s41589-020-0514-0

A gut reaction

<span class=”small-caps”>d</span>-Cycloserine destruction by alanine racemase and the limit of irreversible inhibition

March 16th, 2020 by Cesira de Chiara

Nature Chemical Biology, Published online: 16 March 2020; doi:10.1038/s41589-020-0498-9

d-Cycloserine inactivates alanine racemase by forming an adduct with the pyridoxal 5′-phosphate cofactor, but structural and spectroscopic analyses reveal that reactivation occurs on adduct hydrolysis and product rearrangement to a stable oxime.
  • Posted in Nat Chem Biol, Publications
  • Comments Off on <span class=”small-caps”>d</span>-Cycloserine destruction by alanine racemase and the limit of irreversible inhibition

Atomic structure of potato virus X, the prototype of the <i>Alphaflexiviridae</i> family

March 16th, 2020 by Alessandro Grinzato

Nature Chemical Biology, Published online: 16 March 2020; doi:10.1038/s41589-020-0502-4

A cryo-EM structure of potato virus X shows that virions are formed by repeats of segments of coat protein protomers arranged in a left-handed helical structure and reveals details of the RNA–capsid interactions, which shed light on virus assembly.
  • Posted in Nat Chem Biol, Publications
  • Comments Off on Atomic structure of potato virus X, the prototype of the <i>Alphaflexiviridae</i> family

A new path to tyrosine sulfation

March 16th, 2020 by Chang C. Liu

Nature Chemical Biology, Published online: 16 March 2020; doi:10.1038/s41589-020-0482-4

Tyrosine sulfation is a common post-translational modification known to play critical roles in multiple bioprocesses. A cleverly engineered mammalian expanded genetic code now enables the direct co-translational incorporation of tyrosine sulfates into proteins to study their function in cellular contexts.