IFITM3 directly engages and shuttles incoming virus particles to lysosomes

January 14th, 2019 by Jennifer S. Spence

IFITM3 directly engages and shuttles incoming virus particles to lysosomes

IFITM3 directly engages and shuttles incoming virus particles to lysosomes, Published online: 14 January 2019; doi:10.1038/s41589-018-0213-2

Live-cell imaging and virus trafficking studies show that the host innate immune receptor IFITM3 localizes with endocytic vesicles that fuse with incoming viruses to ultimately enhance their traffic to lysosomes.
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Selective and reversible modification of kinase cysteines with chlorofluoroacetamides

January 14th, 2019 by Naoya Shindo

Selective and reversible modification of kinase cysteines with chlorofluoroacetamides

Selective and reversible modification of kinase cysteines with chlorofluoroacetamides, Published online: 14 January 2019; doi:10.1038/s41589-018-0204-3

Discovery and exploitation of inherent reaction features of chlorofluoroacetamide (CFA) as a warhead such as low off-target activity and reversible reactivity with cysteine enable specific covalent inhibition of targeted kinases.
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Complete reconstitution of the diverse pathways of gentamicin B biosynthesis

January 14th, 2019 by Yeon Hee Ban

Complete reconstitution of the diverse pathways of gentamicin B biosynthesis

Complete reconstitution of the diverse pathways of gentamicin B biosynthesis, Published online: 14 January 2019; doi:10.1038/s41589-018-0203-4

The reconstitution of gentamicin B biosynthesis reveals the existence of multiple new intermediates and branching pathways and enables the identification of factors that contribute to the low levels of the natural product in the native producer.
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Fast bioelectrical switches

January 14th, 2019 by Michaela TerAvest

Fast bioelectrical switches

Fast bioelectrical switches, Published online: 14 January 2019; doi:10.1038/s41589-018-0212-3

Faster-than-transcription control of cellular activities is an important but challenging engineering target. Using split ferredoxins and induced dimerization or conformational changes, newly developed metalloprotein switches provide a fast method to control electron flux.

A chemical–genetic screen identifies ABHD12 as an oxidized-phosphatidylserine lipase

January 14th, 2019 by Dhanashree S. Kelkar

A chemical–genetic screen identifies ABHD12 as an oxidized-phosphatidylserine lipase

A chemical–genetic screen identifies ABHD12 as an oxidized-phosphatidylserine lipase, Published online: 14 January 2019; doi:10.1038/s41589-018-0195-0

Screening with a small-molecule reactive-oxygen-species generator identifies the serine hydrolase enzyme ABHD12 as a lipase for the proapoptotic oxidized phoshatidylserine (ox-PS) lipids, which trigger production of proinflammatory cytokines.
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Discovery of a ZIP7 inhibitor from a Notch pathway screen

January 14th, 2019 by Erin Nolin

Discovery of a ZIP7 inhibitor from a Notch pathway screen

Discovery of a ZIP7 inhibitor from a Notch pathway screen, Published online: 14 January 2019; doi:10.1038/s41589-018-0200-7

A cell-based phenotypic screen identifying inhibitors of Notch signaling led to the discovery of NVS-ZP7-4, which blocks the activity of the zinc transporter SLC39a7 (ZIP7) and induces cell death through an ER stress mechanism.

Protein circuits reprogram cells

January 7th, 2019 by Yiqian Wu

Protein circuits reprogram cells

Protein circuits reprogram cells, Published online: 07 January 2019; doi:10.1038/s41589-018-0210-5

Two protein circuit systems, split-protease-cleavable orthogonal coiled-coil logic (SPOC logic) and circuits of hacked orthogonal modular proteases (CHOMP), have been developed to permit rapid and logic function-based control of mammalian cellular signaling.

A genetics-free method for high-throughput discovery of cryptic microbial metabolites

January 7th, 2019 by Fei Xu

A genetics-free method for high-throughput discovery of cryptic microbial metabolites

A genetics-free method for high-throughput discovery of cryptic microbial metabolites, Published online: 07 January 2019; doi:10.1038/s41589-018-0193-2

A combination of elicitor screening to induce expression of silent biosynthetic gene clusters with imaging mass spectrometry to visualize the resulting metabolome enables the discovery of nine cryptic natural products.
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<i>Legionella</i> effector SetA as a general O-glucosyltransferase for eukaryotic proteins

January 7th, 2019 by Ling Gao

Legionella effector SetA as a general O-glucosyltransferase for eukaryotic proteins

<i>Legionella</i> effector SetA as a general O-glucosyltransferase for eukaryotic proteins, Published online: 07 January 2019; doi:10.1038/s41589-018-0189-y

A chemoenzymatic tagging approach was developed and identified eukaryotic host proteins that are O-glycosylated by SetA from Legionella. The SetA-consensus motif was applied to recombinant proteins yielding a site-specific O-glucosylation method.
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Targeted delivery of nitric oxide via a ‘bump-and-hole’-based enzyme–prodrug pair

December 31st, 2018 by Jingli Hou

Targeted delivery of nitric oxide via a ‘bump-and-hole’-based enzyme–prodrug pair

Targeted delivery of nitric oxide via a ‘bump-and-hole’-based enzyme–prodrug pair, Published online: 31 December 2018; doi:10.1038/s41589-018-0190-5

A NO delivery system that depends on the hydrolysis of an alkyl-galactose-conjugated NO prodrug by an engineered galactosidase developed using a ‘bump-and-hole’ strategy enabled targeted delivery of NO to specific tissues.
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