Architecture of the membrane-bound cytochrome <i>c</i> heme lyase CcmF

May 6th, 2021 by Anton Brausemann

Nature Chemical Biology, Published online: 06 May 2021; doi:10.1038/s41589-021-00793-8

The structure of the heme lyase CcmF, an integral membrane protein, reveals a cavity opening toward the extracellular side to receive heme groups from the chaperone CcmE and a surface groove for guiding the substrate protein during heme attachment.
  • Posted in Nat Chem Biol, Publications
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Genetically encoded multivalent liquid glycan array displayed on M13 bacteriophage

May 6th, 2021 by Mirat Sojitra

Nature Chemical Biology, Published online: 06 May 2021; doi:10.1038/s41589-021-00788-5

Liquid glycan arrays (LiGAs), presented on M13 bacteriophage surface proteins through bioorthogonal chemistry, link surface glycans to genetic barcodes in phage DNA, enabling lectin–glycan interaction profiling by DNA sequencing.
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Circularly permuted LOV2 as a modular photoswitch for optogenetic engineering

May 6th, 2021 by Lian He

Nature Chemical Biology, Published online: 06 May 2021; doi:10.1038/s41589-021-00792-9

A set of LOV2 circular permutants (cpLOV2) have been engineered to expand the existing optogenetic toolbox. cpLOV2 enables the design of both ON- and OFF-switches to control cell signaling, gene expression, cell fate and cancer immunotherapy.
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A molecular trap inside microtubules probes luminal access by soluble proteins

May 3rd, 2021 by Yuta Nihongaki

Nature Chemical Biology, Published online: 03 May 2021; doi:10.1038/s41589-021-00791-w

A chemically inducible technique for trapping cytosolic proteins in microtubules (MTs) in cells reveals that soluble proteins can enter the MT lumen by diffusion, while proteins forming a complex with tubulins can be incorporated at MT plus ends.
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A stop sign for RAS trafficking

April 29th, 2021 by Tian Qiu

Nature Chemical Biology, Published online: 29 April 2021; doi:10.1038/s41589-021-00789-4

N-Ras lipidation cycling is known to regulate its trafficking and function. A new S-depalmitoylation inhibitor induces N-Ras membrane mislocalization, providing insights into N-Ras function and laying the foundation for a new cancer therapeutic strategy.

ABHD17 regulation of plasma membrane palmitoylation and N-Ras-dependent cancer growth

April 29th, 2021 by Jarrett R. Remsberg

Nature Chemical Biology, Published online: 29 April 2021; doi:10.1038/s41589-021-00785-8

ABD957 is a potent and selective inhibitor of the ABHD17 family of depalmitoylases that disrupts N-Ras signaling in human acute myeloid leukemia cells and can synergize with MEK inhibition.
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Engineering AraC to make it responsive to light instead of arabinose

April 26th, 2021 by Edoardo Romano

Nature Chemical Biology, Published online: 26 April 2021; doi:10.1038/s41589-021-00787-6

Engineering of a blue light-inducible AraC dimerization system in E. coli permits light-dependent, rather than arabinose-based, regulation of gene expression from standard PBAD promoters, enabling temporal and spatial control of bacterial systems.

Focal assembly

April 26th, 2021 by Yiyun Song

Nature Chemical Biology, Published online: 26 April 2021; doi:10.1038/s41589-021-00797-4

Focal assembly

Guiding light

April 26th, 2021 by Grant Miura

Nature Chemical Biology, Published online: 26 April 2021; doi:10.1038/s41589-021-00794-7

Guiding light

Designing stable DNAzymes

April 26th, 2021 by Ross Cloney

Nature Chemical Biology, Published online: 26 April 2021; doi:10.1038/s41589-021-00796-5

Designing stable DNAzymes