[ASAP] Understanding and Engineering Distributed Biochemical Pathways in Microbial Communities

November 20th, 2018 by Xinyun Cao, Joshua J. Hamilton, Ophelia S. Venturelli

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Biochemistry
DOI: 10.1021/acs.biochem.8b01006

[ASAP] Hinge–Linker Elements in the AAA+ Protein Unfoldase ClpX Mediate Intersubunit Communication, Assembly, and Mechanical Activity

November 20th, 2018 by Tristan A. Bell, Tania A. Baker, Robert T. Sauer

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Biochemistry
DOI: 10.1021/acs.biochem.8b00907

Polarized displacement by transcription activator-like effectors for regulatory circuits

November 19th, 2018 by Tina Lebar

Polarized displacement by transcription activator-like effectors for regulatory circuits

Polarized displacement by transcription activator-like effectors for regulatory circuits, Published online: 19 November 2018; doi:10.1038/s41589-018-0163-8

The observation that transcription activator-like effectors (TALES) displace TALES bound at adjacent downstream DNA enables engineered regulation of gene expression, displacement of other DNA binding proteins and construction of logic-gated systems.
  • Posted in Nat Chem Biol, Publications
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[ASAP] Binding Affinity and Function of the Extremely Disordered Protein Complex Containing Human Linker Histone H1.0 and Its Chaperone ProTa

November 19th, 2018 by Hanqiao Feng, Bing-Rui Zhou, Yawen Bai

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Biochemistry
DOI: 10.1021/acs.biochem.8b01075

Devilish diatoms

November 14th, 2018 by Caitlin Deane

Devilish diatoms

Devilish diatoms, Published online: 14 November 2018; doi:10.1038/s41589-018-0175-4

Devilish diatoms

Transcriptional recorder

November 14th, 2018 by Yiyun Song

Transcriptional recorder

Transcriptional recorder, Published online: 14 November 2018; doi:10.1038/s41589-018-0174-5

Transcriptional recorder

Expanding the terpenoid kingdom

November 14th, 2018 by Yongjin J. Zhou

Expanding the terpenoid kingdom

Expanding the terpenoid kingdom, Published online: 14 November 2018; doi:10.1038/s41589-018-0167-4

Terpenoids are assembled from five-carbon (C5) units, which limits the available scaffold chemical space for the discovery of bioactive molecules. A combination of enzyme and metabolic engineering now enables biosynthesis of a plethora of non-natural C11 terpenoids.

Disrupting the CD95–PLCγ1 interaction prevents Th17-driven inflammation

November 14th, 2018 by Amanda Poissonnier

Disrupting the CD95–PLCγ1 interaction prevents Th17-driven inflammation

Disrupting the CD95–PLCγ1 interaction prevents Th17-driven inflammation, Published online: 14 November 2018; doi:10.1038/s41589-018-0162-9

The HIV protease inhibitor ritonavir targets the calcium-inducing domain (CID) of CD95 to block interactions with PLCγ1. Peptidomimetics targeting the CD95 CID prevents accumulation of inflammatory Th17 cells observed in systemic lupus erythematosus.
  • Posted in Nat Chem Biol, Publications
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Synthesis of 11-carbon terpenoids in yeast using protein and metabolic engineering

November 14th, 2018 by Codruta Ignea

Synthesis of 11-carbon terpenoids in yeast using protein and metabolic engineering

Synthesis of 11-carbon terpenoids in yeast using protein and metabolic engineering, Published online: 14 November 2018; doi:10.1038/s41589-018-0166-5

Yeast engineered to produce the six-carbon terpene building block 2-methyl-geranyl diphosphate, coupled with terpene synthases engineered to accept this noncanonical substrate, enables the biosynthesis of a collection of new C11 terpenoid scaffolds.
  • Posted in Nat Chem Biol, Publications
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Failure to convert

November 14th, 2018 by Grant Miura

Failure to convert

Failure to convert, Published online: 14 November 2018; doi:10.1038/s41589-018-0172-7

Failure to convert