Repair of a Site-Specific DNA Cleavage: Old-School Lessons for Cas9-Mediated Gene Editing

November 14th, 2017 by Danielle N. Gallagher and James E. Haber

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ACS Chemical Biology
DOI: 10.1021/acschembio.7b00760

Synthetic microbial consortia enable rapid assembly of pure translation machinery

November 13th, 2017 by Fernando Villarreal

Synthetic microbial consortia enable rapid assembly of pure translation machinery

Synthetic microbial consortia enable rapid assembly of pure translation machinery, Published online: 13 November 2017; doi:10.1038/nchembio.2514

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Strains of Escherichia coli, each expressing a subset of the 34 translation machinery proteins, are grown in synthetic microbial consortia to enable the efficient isolation of the full machinery from a single culturing, lysis, and purification procedure.

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Nonimmune cells equipped with T-cell-receptor-like signaling for cancer cell ablation

November 13th, 2017 by Ryosuke Kojima

Nonimmune cells equipped with T-cell-receptor-like signaling for cancer cell ablation

Nonimmune cells equipped with T-cell-receptor-like signaling for cancer cell ablation, Published online: 13 November 2017; doi:10.1038/nchembio.2498

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Engineering of nonimmune cells with a cell-contact sensor and antigen recognition domains enables cell-contact-dependent sensor cell signaling and effector molecule production directed to attack target cells, providing an alternative strategy to chimeric antigen receptor T-cell (CAR-T) technology.

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  • Posted in Nat Chem Biol, Publications
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Top-down characterization of endogenous protein complexes with native proteomics

November 13th, 2017 by Owen S Skinner

Top-down characterization of endogenous protein complexes with native proteomics

Top-down characterization of endogenous protein complexes with native proteomics, Published online: 13 November 2017; doi:10.1038/nchembio.2515

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A multistage tandem mass spectrometry approach enables the application of native proteomics to characterize intact endogenous protein complexes in discovery mode, including covalent modifications as well as noncovalently bound cofactors and ligands.

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Metabolomics-based discovery of a metabolite that enhances oligodendrocyte maturation

November 13th, 2017 by Brittney A Beyer

Metabolomics-based discovery of a metabolite that enhances oligodendrocyte maturation

Metabolomics-based discovery of a metabolite that enhances oligodendrocyte maturation, Published online: 13 November 2017; doi:10.1038/nchembio.2517

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Mass-spectrometry-based metabolomics analysis of oligodendrocyte differentiation led to the identification of an endogenous metabolite, taurine, that enhanced the process of drug-induced OPC differentiation.

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Ultrasensitive optical imaging with lanthanide lumiphores

November 6th, 2017 by Ukrae Cho

Ultrasensitive optical imaging with lanthanide lumiphores

Ultrasensitive optical imaging with lanthanide lumiphores, Published online: 06 November 2017; doi:10.1038/nchembio.2513

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Ultrasensitive imaging of lanthanide chelates is achieved by integrating transreflected illumination, luminescence resonance energy transfer, and time-resolved microscopy.

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Purinyl-cobamide is a native prosthetic group of reductive dehalogenases

November 6th, 2017 by Jun Yan

Purinyl-cobamide is a native prosthetic group of reductive dehalogenases

Purinyl-cobamide is a native prosthetic group of reductive dehalogenases, Published online: 06 November 2017; doi:10.1038/nchembio.2512

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In organohalide-respiring bacteria, reductive dehalogenases cleave carbon–halogen bonds using cobamide prosthetic groups. Desulfitobacterium hafniense uses an unprecedented cobamide variant, which includes unsubstituted purine, for this function.

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Biosynthesis of an Opine Metallophore by Pseudomonas aeruginosa

November 3rd, 2017 by Jeffrey S. McFarlane and Audrey L. Lamb

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Biochemistry
DOI: 10.1021/acs.biochem.7b00804

Large Scale Mutational and Kinetic Analysis of a Self-Hydrolyzing Deoxyribozyme

November 3rd, 2017 by V. Dhamodharan, Shungo Kobori and Yohei Yokobayashi

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ACS Chemical Biology
DOI: 10.1021/acschembio.7b00621

Synthetic beta cells for fusion-mediated dynamic insulin secretion

October 30th, 2017 by Zhaowei Chen

Synthetic beta cells for fusion-mediated dynamic insulin secretion

Synthetic beta cells for fusion-mediated dynamic insulin secretion, Published online: 30 October 2017; doi:10.1038/nchembio.2511

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Synthetic beta cells were fabricated through 'vesicles-in-vesicle' liposomal superstructures equipped with glucose-sensing and membrane-fusion machinery, thus enabling sensing of graded glucose levels and secretion of insulin via fusion processes.

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