Proteostasis: TSA1 to the rescue

July 19th, 2016 by Grant Miura

Nature Chemical Biology 12, 575 (2016). doi:10.1038/nchembio.2138

Author: Grant Miura

Glycomics: Highlights for carbohydrates

July 19th, 2016 by Terry L. Sheppard

Nature Chemical Biology 12, 575 (2016). doi:10.1038/nchembio.2136

Author: Terry L. Sheppard

Antibiotics: I want a new drug

July 19th, 2016 by Joshua M. Finkelstein

Nature Chemical Biology 12, 575 (2016). doi:10.1038/nchembio.2137

Author: Joshua M. Finkelstein

Chaperones: Speedy motion for function

July 19th, 2016 by Hagen Hofmann

Nature Chemical Biology 12, 576 (2016). doi:10.1038/nchembio.2130

Author: Hagen Hofmann

Hsp90 is an energy-consuming molecular chaperone that activates oncogenic proteins in a complicated multi-step reaction. Photoinduced electron transfer (PET) quenching experiments with a fluorescent reporter have now identified molecular transitions at multiple timescales in the chaperone cycle of Hsp90.

Corrigendum: A PHGDH inhibitor reveals coordination of serine synthesis and one-carbon unit fate

July 19th, 2016 by Michael E Pacold

Nature Chemical Biology 12, 656 (2016). doi:10.1038/nchembio0816-656

Author: Michael E Pacold, Kyle R Brimacombe, Sze Ham Chan, Jason M Rohde, Caroline A Lewis, Lotteke J Y M Swier, Richard Possemato, Walter W Chen, Lucas B Sullivan, Brian P Fiske, Steve Cho, Elizaveta Freinkman, Kıvanç Birsoy, Monther-Remaileh Abu, Yoav D Shaul, Chieh Min Liu, Minerva Zhou, Min Jung Koh, Haeyoon Chung, Shawn M Davidson, Alba Luengo, Amy Q Wang, Xin Xu, Adam Yasgar, Li Liu, Ganesha Rai, Kenneth D Westover, Matthew G Vander Heiden, Min Shen, Nathanael S Gray, Matthew B Boxer & David M Sabatini

  • Posted in Nat Chem Biol, Publications
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An oral load of [13C3]glycerol and blood NMR analysis detect fatty acid esterification, pentose phosphate pathway and glycerol metabolism through the tricarboxylic acid cycle in human liver [Lipids]

July 18th, 2016 by Jin, E. S., Sherry, A. D., Malloy, C. R.

Drugs and other interventions for high-impact hepatic diseases often target biochemical pathways such as gluconeogenesis, lipogenesis or the metabolic response to oxidative stress. However, traditional liver function tests do not provide quantitative data about these pathways. In this study, we developed a simple method to evaluate these processes by NMR analysis of plasma metabolites. Healthy subjects ingested [U-13C3]glycerol and blood was dawn at multiple times. Each subject completed three visits under differing nutritional states. High resolution 13C NMR spectra of plasma triacylglycerols and glucose provided new insights into a number of hepatic processes including fatty acid esterification, the pentose phosphate pathway, and gluconeogenesis through the tricarboxylic acid cycle. Fasting stimulated pentose phosphate pathway activity and metabolism of [U-13C3]glycerol in the tricarboxylic acid cycle prior to gluconeogenesis or glyceroneogenesis. Fatty acid esterification was transient in the fasted state, but continuous under fed conditions. We conclude that a simple NMR analysis of blood metabolites provides an important biomarker of pentose phosphate pathway activity, triacylglycerol synthesis and flux through anaplerotic pathways in mitochondria of human liver.
  • Posted in Journal of Biological Chemistry, Publications
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Small-molecule antagonists of germination of the parasitic plant Striga hermonthica

July 18th, 2016 by Duncan Holbrook-Smith

Nature Chemical Biology 12, 724 (2016). doi:10.1038/nchembio.2129

Authors: Duncan Holbrook-Smith, Shigeo Toh, Yuichiro Tsuchiya & Peter McCourt

  • Posted in Nat Chem Biol, Publications
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Targeted inhibition of oncogenic miR-21 maturation with designed RNA-binding proteins

July 18th, 2016 by Yu Chen

Nature Chemical Biology 12, 717 (2016). doi:10.1038/nchembio.2128

Authors: Yu Chen, Fan Yang, Lorena Zubovic, Tom Pavelitz, Wen Yang, Katherine Godin, Matthew Walker, Suxin Zheng, Paolo Macchi & Gabriele Varani

  • Posted in Nat Chem Biol, Publications
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Structural analysis of Notch-regulating Rumi reveals basis for pathogenic mutations

July 18th, 2016 by Hongjun Yu

Nature Chemical Biology 12, 735 (2016). doi:10.1038/nchembio.2135

Authors: Hongjun Yu, Hideyuki Takeuchi, Megumi Takeuchi, Qun Liu, Joshua Kantharia, Robert S Haltiwanger & Huilin Li

  • Posted in Nat Chem Biol, Publications
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Structure and function of the bacterial decapping enzyme NudC

July 18th, 2016 by Katharina Höfer

Nature Chemical Biology 12, 730 (2016). doi:10.1038/nchembio.2132

Authors: Katharina Höfer, Sisi Li, Florian Abele, Jens Frindert, Jasmin Schlotthauer, Julia Grawenhoff, Jiamu Du, Dinshaw J Patel & Andres Jäschke