Polyketide and nonribosomal peptide retro-biosynthesis and global gene cluster matching

October 3rd, 2016 by Chris A Dejong

Nature Chemical Biology 12, 1007 (2016). doi:10.1038/nchembio.2188

Authors: Chris A Dejong, Gregory M Chen, Haoxin Li, Chad W Johnston, Mclean R Edwards, Philip N Rees, Michael A Skinnider, Andrew L H Webster & Nathan A Magarvey

  • Posted in Nat Chem Biol, Publications
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Enzymatic hydrolysis by transition-metal-dependent nucleophilic aromatic substitution

October 3rd, 2016 by Sibel Kalyoncu

Nature Chemical Biology 12, 1031 (2016). doi:10.1038/nchembio.2191

Authors: Sibel Kalyoncu, David P Heaner, Zohre Kurt, Casey M Bethel, Chiamaka U Ukachukwu, Srinivas Chakravarthy, Jim C Spain & Raquel L Lieberman

  • Posted in Nat Chem Biol, Publications
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Cbr1 is a Dph3 reductase required for the tRNA wobble uridine modification

October 3rd, 2016 by Zhewang Lin

Nature Chemical Biology 12, 995 (2016). doi:10.1038/nchembio.2190

Authors: Zhewang Lin, Min Dong, Yugang Zhang, Eunyoung Alisa Lee & Hening Lin

Diphthamide and the tRNA wobble uridine modifications both require diphthamide biosynthesis 3 (Dph3) protein as an electron donor for the iron-sulfur clusters in their biosynthetic enzymes. Here, using a proteomic approach, we identified Saccharomyces cerevisiae cytochrome b5 reductase (Cbr1) as a NADH-dependent reductase for Dph3. The NADH- and Cbr1-dependent reduction of Dph3 may provide a regulatory linkage between cellular metabolic state and protein translation.

  • Posted in Nat Chem Biol, Publications
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Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells

October 3rd, 2016 by Cecilia Lopez-Sambrooks

Nature Chemical Biology 12, 1023 (2016). doi:10.1038/nchembio.2194

Authors: Cecilia Lopez-Sambrooks, Shiteshu Shrimal, Carol Khodier, Daniel P Flaherty, Natalie Rinis, Jonathan C Charest, Ningguo Gao, Peng Zhao, Lance Wells, Timothy A Lewis, Mark A Lehrman, Reid Gilmore, Jennifer E Golden & Joseph N Contessa

  • Posted in Nat Chem Biol, Publications
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The AAA+ FtsH Protease Degrades an ssrA-Tagged Model Protein in the Inner Membrane of Escherichia coli

September 30th, 2016 by Sanjay B. Hari and Robert T. Sauer

TOC Graphic

Biochemistry
DOI: 10.1021/acs.biochem.6b00920

Roger Y. Tsien 1952–2016

September 26th, 2016 by Amy E Palmer

Nature Chemical Biology 12, 887 (2016). doi:10.1038/nchembio.2213

Authors: Amy E Palmer & Jin Zhang

Roger Tsien left us on August 24. His untimely passing has saddened and shocked the scientific community. Roger literally and figuratively brightened our world, illuminated the dark matter of biology, and forever changed our view of the interface of chemistry and biology.

Structural basis for precursor protein–directed ribosomal peptide macrocyclization

September 26th, 2016 by Kunhua Li

Nature Chemical Biology 12, 973 (2016). doi:10.1038/nchembio.2200

Authors: Kunhua Li, Heather L Condurso, Gengnan Li, Yousong Ding & Steven D Bruner

  • Posted in Nat Chem Biol, Publications
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Thermal profiling reveals phenylalanine hydroxylase as an off-target of panobinostat

September 26th, 2016 by Isabelle Becher

Nature Chemical Biology 12, 908 (2016). doi:10.1038/nchembio.2185

Authors: Isabelle Becher, Thilo Werner, Carola Doce, Esther A Zaal, Ina Tögel, Crystal A Khan, Anne Rueger, Marcel Muelbaier, Elsa Salzer, Celia R Berkers, Paul F Fitzpatrick, Marcus Bantscheff & Mikhail M Savitski

We describe a two-dimensional thermal proteome profiling strategy that can be combined with an orthogonal chemoproteomics approach to enable comprehensive target profiling of the marketed histone deacetylase inhibitor panobinostat. The N-hydroxycinnamide moiety is identified as critical for potent and tetrahydrobiopterin-competitive inhibition of phenylalanine hydroxylase leading to increases in phenylalanine and decreases in tyrosine levels. These findings provide a rationale for adverse clinical observations and suggest repurposing of the drug for treatment of tyrosinemia.

  • Posted in Nat Chem Biol, Publications
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Amino-group carrier-protein-mediated secondary metabolite biosynthesis in Streptomyces

September 26th, 2016 by Fumihito Hasebe

Nature Chemical Biology 12, 967 (2016). doi:10.1038/nchembio.2181

Authors: Fumihito Hasebe, Kenichi Matsuda, Taro Shiraishi, Yushi Futamura, Takeshi Nakano, Takeo Tomita, Ken Ishigami, Hikari Taka, Reiko Mineki, Tsutomu Fujimura, Hiroyuki Osada, Tomohisa Kuzuyama & Makoto Nishiyama

  • Posted in Nat Chem Biol, Publications
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Corrigendum: Structural biology: HDAC6 finally crystal clear

September 20th, 2016 by Yanli Liu

Nature Chemical Biology 12, 885 (2016). doi:10.1038/nchembio1016-885

Author: Yanli Liu, Li Li & Jinrong Min