Tbx16 regulates hox gene activation in mesodermal progenitor cells

July 4th, 2016 by Alexander Y Payumo

Nature Chemical Biology 12, 694 (2016). doi:10.1038/nchembio.2124

Authors: Alexander Y Payumo, Lindsey E McQuade, Whitney J Walker, Sayumi Yamazoe & James K Chen

Identification of Cyanobacteriochromes Detecting Far-Red Light

July 2nd, 2016 by Nathan C. Rockwell, Shelley S. Martin and J. Clark Lagarias

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Biochemistry
DOI: 10.1021/acs.biochem.6b00299

Benchmarking of TALE- and CRISPR/dCas9-Based Transcriptional Regulators in Mammalian Cells for the Construction of Synthetic Genetic Circuits

June 28th, 2016 by Tina Lebar and Roman Jerala

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.5b00259
  • Posted in ACS Synthetic Biology, Publications
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Structural basis for halogenation by iron- and 2-oxo-glutarate-dependent enzyme WelO5

June 27th, 2016 by Andrew J Mitchell

Nature Chemical Biology 12, 636 (2016). doi:10.1038/nchembio.2112

Authors: Andrew J Mitchell, Qin Zhu, Ailiena O Maggiolo, Nikhil R Ananth, Matthew L Hillwig, Xinyu Liu & Amie K Boal

  • Posted in Nat Chem Biol, Publications
  • Comments Off on Structural basis for halogenation by iron- and 2-oxo-glutarate-dependent enzyme WelO5

Precursors of tRNAs are stabilized by methylguanosine cap structures

June 27th, 2016 by Takayuki Ohira

Nature Chemical Biology 12, 648 (2016). doi:10.1038/nchembio.2117

Authors: Takayuki Ohira & Tsutomu Suzuki

Loss of protein association causes cardiolipin degradation in Barth syndrome

June 27th, 2016 by Yang Xu

Nature Chemical Biology 12, 641 (2016). doi:10.1038/nchembio.2113

Authors: Yang Xu, Colin K L Phoon, Bob Berno, Kenneth D'Souza, Esthelle Hoedt, Guoan Zhang, Thomas A Neubert, Richard M Epand, Mindong Ren & Michael Schlame

  • Posted in Nat Chem Biol, Publications
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Preincubation of t-SNAREs with Complexin I Increases Content-Mixing Efficiency

June 24th, 2016 by Jaewook Kim, Yicheng Zhu and Yeon-Kyun Shin

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Biochemistry
DOI: 10.1021/acs.biochem.6b00114

NEDD4L Catalyzes Ubiquitination of PIK3CA and Regulates PI3K-AKT Signaling [Signal Transduction]

June 23rd, 2016 by Wang, Z., Dang, T., Liu, T., Chen, S., Li, L., Huang, S., Fang, M.

Oncogenic PIK3CA (p110α), the catalytic subunit of class IA PI3K, plays a major role in PI3K related cancer progression. The mechanisms underlying the dynamic regulation of PIK3CA protein levels remain unknown. Here we demonstrated that PIK3CA is regulated by polyubiquitination. We identified NEDD4L as the E3 ligase that catalyzes PIK3CA polyubiquitination, leading to its proteasome-dependent degradation. NEDD4L ubiquitinates both the free and regulatory subunit-bound PIK3CA, but does not ubiquitinate the regulatory subunit of PI3K. Overexpression of NEDD4L accelerates the turnover rate of PIK3CA whereas suppression of NEDD4L results in not only the accumulation of PIK3CA but also a paradoxical decrease of AKT activation. Thus, we propose that NEDD4L negatively regulates PIK3CA protein levels via ubiquitination and is required for the maintenance of PI3K-AKT signaling pathway.

Cooperation of local motions in the Hsp90 molecular chaperone ATPase mechanism

June 20th, 2016 by Andrea Schulze

Nature Chemical Biology 12, 628 (2016). doi:10.1038/nchembio.2111

Authors: Andrea Schulze, Gerti Beliu, Dominic A Helmerich, Jonathan Schubert, Laurence H Pearl, Chrisostomos Prodromou & Hannes Neuweiler

  • Posted in Nat Chem Biol, Publications
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A family of metal-dependent phosphatases implicated in metabolite damage-control

June 20th, 2016 by Lili Huang

Nature Chemical Biology 12, 621 (2016). doi:10.1038/nchembio.2108

Authors: Lili Huang, Anna Khusnutdinova, Boguslaw Nocek, Greg Brown, Xiaohui Xu, Hong Cui, Pierre Petit, Robert Flick, Rémi Zallot, Kelly Balmant, Michael J Ziemak, John Shanklin, Valérie de Crécy-Lagard, Oliver Fiehn, Jesse F Gregory, Andrzej Joachimiak, Alexei Savchenko, Alexander F Yakunin & Andrew D Hanson

  • Posted in Nat Chem Biol, Publications
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