ACS DIVISION OF BIOLOGICAL CHEMISTRY

November 6th, 2017 by Jun Yan

Purinyl-cobamide is a native prosthetic group of reductive dehalogenases

Purinyl-cobamide is a native prosthetic group of reductive dehalogenases, Published online: 06 November 2017; doi:10.1038/nchembio.2512

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In organohalide-respiring bacteria, reductive dehalogenases cleave carbon–halogen bonds using cobamide prosthetic groups. Desulfitobacterium hafniense uses an unprecedented cobamide variant, which includes unsubstituted purine, for this function.

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November 6th, 2017 by Ukrae Cho

Ultrasensitive optical imaging with lanthanide lumiphores

Ultrasensitive optical imaging with lanthanide lumiphores, Published online: 06 November 2017; doi:10.1038/nchembio.2513

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Ultrasensitive imaging of lanthanide chelates is achieved by integrating transreflected illumination, luminescence resonance energy transfer, and time-resolved microscopy.

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November 3rd, 2017 by Jeffrey S. McFarlane and Audrey L. Lamb

November 3rd, 2017 by V. Dhamodharan, Shungo Kobori and Yohei Yokobayashi

October 30th, 2017 by Yi Qu

Small molecule promotes β-catenin citrullination and inhibits Wnt signaling in cancer

Small molecule promotes β-catenin citrullination and inhibits Wnt signaling in cancer, Published online: 30 October 2017; doi:10.1038/nchembio.2510

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The small molecule nitazoxanide (NTZ) was identified as a Wnt inhibitor by promoting protein citrullination of β-catenin through increased cytosolic calcium and PAD2 protein stabilization. β-catenin citrullination results in proteasomal degradation.

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October 30th, 2017 by David Sychantha

PatB1 is an O-acetyltransferase that decorates secondary cell wall polysaccharides

PatB1 is an <i>O</i>-acetyltransferase that decorates secondary cell wall polysaccharides, Published online: 30 October 2017; doi:10.1038/nchembio.2509

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A chemically synthesized analog of Bacillus cereus secondary cell wall polysaccharide (SCWP) facilitates the identification of PatB1 as a SCWP O-acetyltransferase, and the structure of PatB1 provides insights into its catalytic mechanism.

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October 30th, 2017 by Zhaowei Chen

Synthetic beta cells for fusion-mediated dynamic insulin secretion

Synthetic beta cells for fusion-mediated dynamic insulin secretion, Published online: 30 October 2017; doi:10.1038/nchembio.2511

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Synthetic beta cells were fabricated through 'vesicles-in-vesicle' liposomal superstructures equipped with glucose-sensing and membrane-fusion machinery, thus enabling sensing of graded glucose levels and secretion of insulin via fusion processes.

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October 27th, 2017 by Jonathan Lai, Zhaleh Ghaemi and Zaida Luthey-Schulten

October 27th, 2017 by Srinivasaraghavan Kannan, Anders Poulsen, Hai Yan Yang, Melvyn Ho, Shi Hua Ang, Tan Sum Wai Eldwin, Duraiswamy Athisayamani Jeyaraj, Lohitha Rao Chennamaneni, Boping Liu, Jeffrey Hill, Chandra S. Verma and Kassoum Nacro

October 27th, 2017 by Aaron G. Bart, Emily E. Scott

The small heme-containing protein cytochrome b5 can facilitate, inhibit, or have no effect on cytochrome P450 catalysis, often in a P450-dependent and substrate-dependent manner that is not well understood. Herein solution NMR was used to identify b5 residues interacting with different human drug-metabolizing P450 enzymes. NMR results revealed that P450 enzymes bound to either b5 α4-5 (CYP2A6 and CYP2E1) or this region and α2-3 (CYP2D6 and CYP3A4) and suggested variation in the affinity for b5. Mutations of key b5 residues suggest that not only are different b5 surfaces responsible for binding different P450 enzymes, but that these different complexes are relevant to the observed effects on P450 catalysis.