Bacterial Membranes: Tear down this wall

April 13th, 2017 by Caitlin Deane

Nature Chemical Biology 13, 451 (2017). doi:10.1038/nchembio.2366

Author: Caitlin Deane

Optogenetics: Lighting up kinases

April 13th, 2017 by Grant Miura

Nature Chemical Biology 13, 451 (2017). doi:10.1038/nchembio.2365

Author: Grant Miura

Mechanistic insights into energy conservation by flavin-based electron bifurcation

April 10th, 2017 by Carolyn E Lubner

Nature Chemical Biology 13, 655 (2017). doi:10.1038/nchembio.2348

Authors: Carolyn E Lubner, David P Jennings, David W Mulder, Gerrit J Schut, Oleg A Zadvornyy, John P Hoben, Monika Tokmina-Lukaszewska, Luke Berry, Diep M Nguyen, Gina L Lipscomb, Brian Bothner, Anne K Jones, Anne-Frances Miller, Paul W King, Michael W W Adams & John W Peters

  • Posted in Nat Chem Biol, Publications
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CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters

April 10th, 2017 by Mingzi M Zhang

Nature Chemical Biology 13, 607 (2017). doi:10.1038/nchembio.2341

Authors: Mingzi M Zhang, Fong Tian Wong, Yajie Wang, Shangwen Luo, Yee Hwee Lim, Elena Heng, Wan Lin Yeo, Ryan E Cobb, Behnam Enghiad, Ee Lui Ang & Huimin Zhao

Here we report an efficient CRISPR–Cas9 knock-in strategy to activate silent biosynthetic gene clusters (BGCs) in streptomycetes. We applied this one-step strategy to activate multiple BGCs of different classes in five Streptomyces species and triggered the production of unique metabolites, including a novel pentangular type II polyketide in Streptomyces viridochromogenes. This potentially scalable strategy complements existing activation approaches and facilitates discovery efforts to uncover new compounds with interesting bioactivities.

  • Posted in Nat Chem Biol, Publications
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Measurement of In Vivo Protein Binding Affinities in a Signaling Network with Mass Spectrometry

April 5th, 2017 by Mumun Gencoglu, Alexander Schmidt and Attila Becskei

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.6b00282

AtaT blocks translation initiation by N-acetylation of the initiator tRNAfMet

April 3rd, 2017 by Dukas Jurėnas

Nature Chemical Biology 13, 640 (2017). doi:10.1038/nchembio.2346

Authors: Dukas Jurėnas, Sneha Chatterjee, Albert Konijnenberg, Frank Sobott, Louis Droogmans, Abel Garcia-Pino & Laurence Van Melderen

  • Posted in Nat Chem Biol, Publications
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Phosphorylated glycosphingolipids essential for cholesterol mobilization in Caenorhabditis elegans

April 3rd, 2017 by Sebastian Boland

Nature Chemical Biology 13, 647 (2017). doi:10.1038/nchembio.2347

Authors: Sebastian Boland, Ulrike Schmidt, Vyacheslav Zagoriy, Julio L Sampaio, Raphael F Fritsche, Regina Czerwonka, Tilo Lübken, Jakob Reimann, Sider Penkov, Hans-Joachim Knölker & Teymuras V Kurzchalia

  • Posted in Nat Chem Biol, Publications
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A Glucose-Sensing Toggle Switch for Autonomous, High Productivity Genetic Control

March 30th, 2017 by William Bothfeld, Grace Kapov and Keith E. J. Tyo

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.6b00257

The Role of SufS Is Restricted to Fe–S Cluster Biosynthesis in Escherichia coli

March 30th, 2017 by Martin Bühning, Angelo Valleriani and Silke Leimkühler

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Biochemistry
DOI: 10.1021/acs.biochem.7b00040

Simultaneous Measurement of Glucose-6-phosphate 3-Dehydrogenase (NtdC) Catalysis and the Nonenzymatic Reaction of Its Product: Kinetics and Isotope Effects on the First Step in Kanosamine Biosynthesis

March 29th, 2017 by Natasha D. Vetter and David R. J. Palmer

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Biochemistry
DOI: 10.1021/acs.biochem.7b00079